• BMB Reports
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• v.32 no.2
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• pp.119-126
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• 1999

The SH3 domain of PLC-${\gamma}1$ has been known to induce DNA synthesis. However, little is known about the putative effector proteins that associate with the domain. In this report, we provide evidence that the SH3 domain of PLC-${\gamma}1$ associates with Shc, which has been implicated in the activation of p21Ras in response to many growth factors. The association between Shc and PLC-${\gamma}1$ is enhanced either by v-Src-induced transformation or EGF-stimulation in vivo and in vitro. Furthermore, from transient expression studies with COS-7 cells, we show that the SH3 domain of PLC-${\gamma}1$ is required for association with Shc in vivo, whereas tyrosyl phosphorylation of PLC-${\gamma}1$ is not. Taken together, we suggest that Shc might be involved in the PLC-${\gamma}1$-mediated signaling pathway.

• Bulletin of the Korean Chemical Society
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• v.34 no.4
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• pp.1089-1095
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• 2013

The tumor necrosis factor-receptor 1 (TNFR1)-associated death domain protein (TRADD) contains an N-terminal TRAF binding domain and a C-terminal death domain. TRADD is known to interact directly with TNF receptor 2 (TNFR2) and the Fas-associated death domain protein (FADD), which are signal transducers that activate NF-${\kappa}B$ and induce apoptosis, respectively. To date, there has been no structural information on the TRADD and FADD death domain (DDs) complex. In this study, the death domains of TRADD and FADD were co-expressed and purified from Escherichia coli for structural characterization. We found that human TRADD (hTRADD) interacted strongly with mouse FADD (mFADD) via their DDs and interacted weakly with human FADD (hFADD)-DD. Moreover, the structures of the TRADD-DD:FADD-DD complexes were separately modeled from predicted structures in the protein data bank (PDB). The results of this study will have important applications in human diseases such as cancer, AIDS, degenerative and autoimmune diseases, and infectious diseases.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 1997.04a
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• pp.257-264
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• 1997

Seismic responses of the Hualien large scale seismic test model on a layered soil site are estimated for two recorded earthquakes and the analysis results are then compared and evaluated with the recorded responses. The method adopted for the analysis is based on substructuring method using a lumped parameter model in both the frequency and time domain. the study results indicate that the proposed method can reasonably estimate the earthquake responses of a soil-structure interaction system for engineering purposes.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1995.04a
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• pp.81-81
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• 1995

It is well known that Src Homology 2(SH2) domain in many intracellular signal transduction proteins is very important. The domain has about 100 amino acid residues and bind phosphotyrosine-containing peptide with high affinity and specificity. Lck SH2 domain is a Src-like, lymphocyte-specific tyrosine kinase. An 11-residue phosphopeptide derived from the hamster polvoma middle-T antigen, EPQp YEEIPIYL, binds with an 1 nM dissociation constant to Lck SH2 domain. And it is known that the phosphotyrosine and isoleucine residues of the peptide are tightly bound by two well-defined pockets on Lck SH2 domain's surface. To investigate the conformational changes during complexation of SH2 domain with phosphopeptide we have performed the molecular dynamics simulation for Lck SH2 domain with peptide and peptide-free form at look in aqueous solution. More than 3000 water molecules were incorporated to solvate Lck SH2 domain and peptide. Periodic boundary condition has been applied in molecular dynamics simulation. Data analysis with the results of that simulation shows that the phosphopeptide makes primary interaction with the Lck SH2 domain at six central residues, The comparison of the complexed and uncomplexed SH2 domain structures in solution has revealed only relatively small change. But the hydrophilic and hydrophobic pockets in the protein surface show the conformational changes in spite of the small structural difference between the complex and peptide-free forms.

• Archives of Pharmacal Research
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• v.21 no.6
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• pp.707-711
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• 1998

The (Na,K)ATPase is responsible for generating the ionic gradients and membrane potentials by the exchange of intracellular $Na^+$ for $K^+$. It has been recentl y shown that (Na,K)ATPase is involved in the exocytic pathway of basic fibroblast growth factor (bFGF), although it is not known that bFGF is secreted to the outside of cell through direct interaction with (Na,K) ATPase. To understand the role for (Na,K)ATPase in the secretary pathway of bFGF, we have sought to identify the cytoplasmic domains of the alpha1 isoform of (Na,K)ATPase interacting with bFGF by yeast two-hybrid system. We have also investigated the interaction between the alpha2 isoform of (Na,K)ATPase and bFGF to find out whether the interaction is isoform-specific. We found that none of the cytoplasmic domains of (Na,K)ATPase isoforms interacted with bFGF. The result suggests that the interaction between bFGF and (Na,K)ATPase might be indirect, thus requiring other proteins which are involved in the formation of protein complexes for the interaction, although we cannot exclude the possibility that the interaction requires the element of the whole alpha subunit structure that was not present in the isolated alpha subunit cytoplasmic domains.

• Interaction and multiscale mechanics
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• v.6 no.4
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• pp.339-356
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• 2013

This paper investigates the optimized parameters of Tuned Mass Dampers (TMDs) for vibration control of high-rise structures including Soil Structure Interaction (SSI). The Artificial Bee Colony (ABC) method is employed for optimization. The TMD Mass, damping coefficient and spring stiffness are assumed as the design variables of the controller; and the objective is set as the reduction of both the maximum displacement and acceleration of the building. The time domain analysis based on Newmark method is employed to obtain the displacement, velocity and acceleration of different stories and TMD in response to 6 types of far field earthquakes. The optimized mass, frequency and damping ratio are then formulated for different soil types; and employed for the design of TMD for the 40 and 15 story buildings and 10 different earthquakes, and well results are achieved. This study leads the researchers to the better understanding and designing of TMDs as passive controllers for the mitigation of earthquake oscillations.

• Animal cells and systems
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• v.13 no.3
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• pp.283-288
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• 2009

The ubiquitous plasma membrane $Na^+/H^+$ exchanger 1 (NHE1) is rapidly activated in response to various extracellular stimuli and maintains normal cytoplasmic pH. Yeast two-hybrid screening was used in order to identify proteins interacting with NHE1 using its cytoplasmic domain as a bait from HeLa cDNA library. One of the interacting cDNA clones was human Lactate dehydrogenase B (LDHB). In vitro translated LDHB was pulled down together with GST-NHE1.cd protein in the GST pull down assay, confirming the interaction in vitro. LDHB antibody immunoprecipitated endogenous LDHB together with NHE1 from H9c2 cells, validating cellular interaction between NHE1 and LDHB. Subsequent analysis revealed that the overexpression of LDHB increased intracellular PH, implying opening of the NHE1 transporter. Moreover, overexpression of LDHB activated caspase 3 and induced cell death, consistent with the expected phenotype of hyper-activation of NHE1. Collectively, our data indicate that LDHB modulates NHE1 activity via physical interaction.

• Interaction and multiscale mechanics
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• v.1 no.1
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• pp.157-175
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• 2008

A brief review of the research works on ground vibrations caused by trains moving in underground tunnels is first given. Then, the finite/infinite element approach for simulating the soil-tunnel interaction system with semi-infinite domain is summarized. The tunnel is assumed to be embedded in a homogeneous half-space or stratified soil medium. The train moving underground is modeled as an infinite harmonic line load. Factors considered in the parametric studies include the soil stratum depth, damping ratio and shear modulus of the soil with or without tunnel, and the thickness of the tunnel lining. As far as ground vibration is concerned, the existence of a concrete tunnel may somewhat compensate for the loss due to excavation of the tunnel. For a soil stratum resting on a bedrock, the resonance peak and frequency of the ground vibrations caused by the underground load can be rather accurately predicted by ignoring the existence of the tunnel. Other important findings drawn from the parametric studies are given in the conclusion.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2002.09a
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• pp.98-105
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• 2002

The effects of the base-isolation system and elastic soil foundation on the behavior of a liquid storage tank are studied. To evaluate the seismic response of liquid storage tank accurately, the coupled dynamic system considering base isolation and soil interaction problem is formulated in time domain. Results show that the base isolation system reduces effectively the radial displacements, base shears, overturning moments, axial resultant stresses and the hydrodynamic pressure by providing flexibility and energy dissipation capability. Base Isolation may, however, increase the relative liquid sloshing amplitude due to the effect of liquid-structure interaction and cause excessive large relative displacements between structure and foundation. In addition to base-isolator, the dynamic behavior of liquid storage tank is related to the flexibility of base foundation, so the analysis of soil-structure interaction s achieved.

• Nuclear Engineering and Technology
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• v.33 no.5
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• pp.539-546
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• 2001

Systematic procedure of developing radionuclide release scenarios was established based on FEP list and Interaction Matrix for near-surface LILW repository. FEPs were screened by experts＇review in terms of domestic situation and combined into scenarios on the basis of Interaction Matrix analysis. Under the assumption of design scenario, The system domain was divided into three sections: Near-field, Far-field and Biosphere. Sub-scenarios for each section were developed, and then scenarios for entire system were built up with sub-scenarios of each section. Finally, sixteen design scenarios for near-surface repository were evaluated A reference scenario and other noteworthy scenarios were selected through experts＇scenario screening.