• The Journal of Korean Obstetrics and Gynecology
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• v.26 no.2
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• pp.1-16
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• 2013

Objectives: This study was performed to evaluate the effect of Dokhwalgisaengtang-gami water extract(DGG) on osteoporosis. Methods: The osteoclastogenesis and gene expression were determined in RANKL-stimulated RAW 264.7 cell. And osteoblastogenesis was also determined in rat calvarial cell. Results: The results were summarized as followes. 1. DGG decreased the number of TRAP positive cell in RANKL-stimulated RAW 264.7 cell. 2. DGG inhibited TRAP activity in RANKL-stimulated RAW 264.7 cell. 3. DGG decreased the expression of NAFTc1, MITF in RANKL-stimulated RAW 264.7 cell. 4. DGG increased the expression of iNOS, COX-2, IL-6 in RANKL-stimulated RAW 264.7 cell. 5. DGG decreased the expression of cathepsin K, MMP-9, TRAP in RANKL-stimulated RAW 264.7 cell. 6. DGG increased cell proliferation of rat calvarial cell. 7. DGG increased ALP activity in rat calvarial cell 8. DGG increased bone matrix protein, collagen synthesis and nodule formation in rat calvarial cell. Conclusions: It is concluded that DGG might decrease the bone resorption resulted from decrease of osteoclast differentiation and it's related gene expression. And DGG might increase the bone formation resulted from increase of osteoblast function.

• Journal of Pharmacopuncture
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• v.3 no.2
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• pp.169-189
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• 2000

To investigate effects of vanous herbal-acupuncture, which were Daebangpungtang, Oyaksungisan, Dokhwalgisaengtang, and Binsosan on Adjuvant Arthritis in rats, the edema rate, the number of WBC, the quantity of TNF-${\alpa}$, COX -2, and IL-6, and histological test of the muscular tissue were measured in the arthritis part. After elicilating arthritis of Sprague Dawely(SD) rats by injection of Freund's complets adjuvant for 2 weeks, saline was injected for the control group, Daebangpungtang acua-acupuncture was injected for the Daebangpungtang group, Oyaksungisan acua-acupuncture was injected for the Oyaksungisan group, Dokhwalgisaengtang acua-acupuncture was injected for the Dokhwalgisaengtang group, and Binsosan acua-acupuncture was injected for the Binsosan group during 30days. Selected point was ST35 in all groups. 1. The number of WBC was $8.24{\pm}0.51$($10^3$/ml) in the normal group and $27.35{\pm}3.51$($10^3$/ml) in the control group. the Daebangpungtang group and the Oyaksungisan group decreased each $20.42{\pm}2.75$($10^3$/ml) and $19.78{\pm}4.99$($10^3$/ml) in the Exp.ll group. This fact showed that the group Exp.ll was more effective than the control group effectively. (P<0.05) 2. The volume of the paw were checked. The volume of paw was $0.62{\pm}0.11mm in the control group and $0.45{\pm}0.08$mm in the Daebangpungtang group and $0.47{\pm}0.07$mm in the Oyaksungisan group, the swelling of the paw was restricted significantly in the Daebangpungtang and Oyaksungisan group(P<0.05) 3. The bands of the TNF-${\alpa}$, COX -2 in the muscular tissue of the control group wcre certain and thick than other groups(except normal group), and the band of the IL-6 in the muscular tissue of all group(except normal group) were similar forms. 4. In histological finding, because of severe inflammatory reaction, remarkably irregular tissue and large amount of inflammatory cells were found in thc control and Dokhwalgisaengtang group. But the Oyaksungisan and Daebangpul1gtang group showed small amount of inflammatory cells, the refrained inflammatory state and even recovering state. From these results, it is showed Oyaksugisan and Daebangpungtang acua-acupuncturc refrain inflammatory reaction and muscular tissue necrosis in SD rats paw were induced by Freund's complete adjuvant

• The Journal of Korean Medicine
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• v.17 no.2 s.32
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• pp.117-132
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• 1996

The study of Jungpung(中風)'s section in Chungumyobang (千金要方, 諸風門) by Sonsamak(孫思邈) showed the following results. 1. The cause of Jungpung(中風) in Jungpung(中風)'s section in Chungumyobang (千金要方, 諸風門) was quotated from the theory of Naekyung(內經) -Pung(風) evil from the external environment invade on Hu(虛) of human body(外來風邪, 內虛邪中). 2. At Jungpung(中風)'s section in Chungumyobang (千金要方, 諸風門), he devided into the four classes of Jungpung(中風)'s symptom-Pyungo, Pungbi, Pungyi, Pungbi(偏枯, 風?, 風懿, 風痺)-, and this classification had a great influence on the next generations. 3. At Jungpung(中風)'s section in Chungumyobang (千金要方, 諸風門), he understanded that changes of Jungpung(中風)'s pathology depended on the Eum-Yang-Han-Yul-Hu-Shil(陰陽寒熱虛實) of the patients. 4. At Jungpung(中風)'s section in Chungumyobang (千金要方, 諸風門), he gathered and rearranged various prescription which accumulated the clinical experiences during the period from Naekyung(內經), Sanghan(傷寒) times, to Tang(唐)'s dynasty. There were major Jungpung(中風)'s prescription -Sosokmyungtang(小續命湯), Daesokmyungtang(大續命湯), Jukryuktang(竹瀝湯), Jihwangjun(地黃煎), Dokhwalgisaengtang(獨活寄生湯) etc. And the principle of treatment was the remedy method of removing evil through sweating(發汗祛邪法). 5. Chungumyobang(千金要方) showed the development of quality, quantity in their prescription comparing the fomer ages. And the method of classification of Jungpung(中風)'s symptom had a great influence on the next generation in clinical aspect. But the basic theory in Chungumyobang(千金要方) coudn't get out of the limits of Naekyung(內經), Gumgueyoryak(金?要略).