• 한국식품영양학회지
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• 제25권3호
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• pp.529-537
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• 2012

고구마의 새로운 이용방법으로 고구마의 기능성을 활용한 고구마 된장을 제조하였다. 고구마 페이스트와 대두분말을 1:1로 혼합하여 펠렛을 제조한 다음 황국균을 접종, 배양하여 효소활성이 높은 고구마 황국을 제조하였고, 제조한 신황미와 신자미 황국(20, 45%)에 증자대두와 식염 10%를 혼합, 60일간 발효 숙성하여 저염 고구마 된장을 제조하였다. 고구마 된장에는 감칠맛 성분인 글루탐산이 일반된장보다 많은 양 함유되어 있었고 숙성에 의해 항산화활성이 증가하였으며, DPPH 라디칼 소거활성($EC_{50}$)은 신자미 황국을 45% 혼합한 신자미 된장에서 0.9 mg으로 가장 높았다. 관능평가에서 신황미 황국을 45% 혼합한 신황미 된장의 선호도가 가장 좋았다.

• Preventive Nutrition and Food Science
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• 제14권2호
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• pp.134-141
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• 2009

The changes in components and biological activities of doenjang samples prepared with black soybeans and fermented with Bacillus subtilis SCB were investigated. The amino nitrogen (A-N) contents of samples increased with increasing black soybean content. A doenjang product made using a 1:1 ratio of soybeans-black soybeans showed a maximum level of genistein and daidzein isoflavones ($1111.6{\mu}g/g$) at 110 days of fermentation, along with decreasing contents of genistin and daidzin due to the conversion to aglycones. The black soybean-only doenjang sample showed higher protease activity, including caseinolytic and fibrinolytic enzyme activities, than the other samples, and had relatively higher polyphenol content and DPPH radical scavenging activity. Therefore, doenjang made with additions of black soybeans and fermented by B. subtilis SCB may have improved physiological properties, suggesting this to be a valuable method of preparation.

• Journal of Microbiology and Biotechnology
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• 제26권4호
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• pp.666-674
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• 2016

The bacterial strains were screened as potential starters for fermenting low-salt doenjang (a Korean traditional fermented soybean paste) using Korean doenjang based on proteolytic and antipathogenic activities under 6.5-7.5% NaCl conditions. Phylogenetic analysis based on 16S rRNA gene sequences showed that they all belonged to the genus Bacillus. Proteolytic and antipathogenic activities against Escherichia coli, Bacillus cereus, Staphylococcus aureus, Listeria monocytogenes, and Aspergillus flavus, as well as fibrinolytic, amylase, and cellulase activities of the 10 strains were quantitatively evaluated. Of these, strains D2-2, JJ-D34, and D12-5 were selected, based on their activities. The functional, phenotypic, and safety-related characteristics of these three strains were additionally investigated and strains D2-2 and D12-5, which lacked antibiotic resistance, were finally selected. Strains D2-2 and D12-5 produced poly-γ-glutamic acid and showed various enzyme activities, including α-glucosidase and β-glucosidase. Growth properties of strains D2-2 and D12-5 included wide temperature and pH ranges, growth in up to 16% NaCl, and weak anaerobic growth, suggesting that they facilitate low-salt doenjang fermentation. Strains D2-2 and D12-5 were not hemolytic, carried no toxin genes, and did not produce biogenic amines. These results suggest that strains D2-2 and D12-5 can serve as appropriate starter cultures for fermenting low-salt doenjang with high quality and safety.

• 산업식품공학
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• 제15권4호
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• pp.318-323
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• 2011

염 함량을 달리한 된장을 초고압 처리하여 미생물 저감특성을 조사함으로써 된장, 특히 저염 된장의 저장성을 높이기 위하여 초고압기술의 적용가능성을 검토하였다. 초고압처리에 의한 된장 미생물 사멸효과는 전반적으로 낮은것으로 나타났으며, 초고압처리에 의한 미생물 사멸효과는 염 함량에 반비례하는 경향을 보였다. 미생물 종류에 따른 초고압사멸효과는 곰팡이, 효모, 세균 순으로 낮아졌다. 염 함량 10% 이하의 저염 된장은 6500 기압에서 40 분간 초고압처리에 의해 곰팡이와 효모가 거의 사멸되었으며, 초고압처리는 저염 된장의 유통기간 향상에 기여할 것으로 기대되었다.

• Journal of Microbiology and Biotechnology
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• 제26권9호
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• pp.1533-1541
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• 2016

Samples of doenjang (a fermented soybean paste) were prepared with different types of salts; purified salt (PS), 3-year-aged solar salt (SS3), 1-year-aged solar salt (SS1), and bamboo salt (BS, 3^rd processing product). For starter doenjang samples, selected starters comprising two bacilli, one yeast, and one fungus were inoculated, whereas for non-starter doenjang samples, microorganisms present in rice straw were inoculated after enrichment. The doenjang samples were fermented for 13 weeks at 25℃. During the fermentation period, SS and BS doenjang samples showed higher bacilli counts as well as much lower yeast counts than PS doenjang. At 13 weeks, yeast counts of starter doenjang samples were 7.75, 5.69, 6.08, and 4.74 log CFU/g for PS, SS3, SS1, and BS doenjang, respectively. For non-starter doenjang samples, counts were 7.17, 5.05, 5.92, and 4.54 log CFU/g for PS, SS3, SS1, and BS doenjang, respectively. SS and BS promoted growth of bacilli but inhibited growth of yeasts compared with PS. Debaryomyces hansenii was the dominant yeast in PS doenjang, whereas Candida guilliermondii and Pichia sorbitophila were dominant in SS and BS doenjang. In the sensory evaluation, SS and BS doenjang scored better than PS doenjang. In conclusion, SS and BS seem better than PS for production of high-quality doenjang.

• 한국식품영양과학회지
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• 제19권2호
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• pp.156-162
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• 1990

Antimutagenic effect of doenjag (Korean fermented soy paste) on mutagenesis induced by aflatoxin B1 (AFB1) in Salmonella typhimurium strains TA98 and TA100 was studied. AFB1 revealed maximum mutagenicity at dose level of 1 $\mu$g/plate with metabolic activation system in both strains. Strong antiutagenic activity toward AFB1 was completely inhibited at the level of 50% of the doenjang extract. At the same concentration 64-66% and 39-53% of the AFB1 induced mutageneses were blocked when the methanol extracts of raw and cooked soybeans were added in the system respectively Raw soybeans showed higher ihhibition rate to the mutagenicity than cooked soybeans but the fermented soybeans(doenjang) was the most effective (p<0.05) Other soybean fermented foods such as commercial doenjang natto and miso were also exhibited some antimutagenic activities however the traditional doenjang was the most effective and then commercial doenjang. Natto and miso were less effective.

• Food Science and Biotechnology
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• 제17권1호
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• pp.208-211
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• 2008

Previously, it has been reported that Aspergillus oryzae can efficiently degrade unpleasant odor components such as butyric acid and 3-methyl butanoic acid from meju, a major ingredient in both Korean soybean paste (doenjang) and soy sauce. In this study, the optimal manufacturing conditions for the production of superior quality Korean soybean paste and soy sauce were determined. Specifically, A. oryzae AJ 100 was utilized to improve the flavor of these products. Mixtures of Korean soybean paste and A. oryzae AJ 100 culture (2 : 1), and of Korean soy sauce and A. oryzae AJ 100 culture (5 : 1), were incubated for 2 weeks at $30^{\circ}C$, and showed improved flavor. Butyric acid and 3-methyl butanoic acid were clearly degraded under these culture conditions.

• 한국식품영양과학회지
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• 제28권2호
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• pp.458-463
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• 1999

Methanol extract and its fraction of traditional fermented soybean paste(doenjang) from Soonchang area were studied for growth suppression on the various tumor cells and suppression components, by using HPLC and GC were analysed. Hexane fraction of methanol extract was indicated 79%, 76%, 67%, 66%, 78% of growth suppression on L1210, P338D1, HepG2, WiDr and SNU 1 tumor cells, respectively. Ethylacetate fraction of methanol extract also showed 81%, 75%, 75%, 76% and 82% of growth suppression on the same tumor cells, respectively. Peak 8 obtained from HPLC of ethylacetate fraction indicated 81%, 77%, 77%, 75% and 79% of growth suppression on the same tumor cells and identified as a genistein, by comparing with standard one by HPLC analysis. Hexane fraction of methanol extract contained oleic acid, linoleic acid and palmitic acid.

• 한국식품과학회지
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• 제36권2호
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• pp.202-210
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• 2004

된장과 순된장에 멸치, 마늘, 고춧가루, 파를 부재료로 첨가하여 찌개로 가열 조리할 때 첨가재료에 의하여 생성된 향기성분 및 그 향기성분 생성에 영향을 미치는 인자를 알아보고자 하였다. 그리고 관능검사를 통하여 된장찌개의 향기성분의 특성에 관여하는 주요성분을 알아보았다. 된장의 향기성분에는 aldehyde류와 ester류의 향기성분이 많았으나 순된장의 향기성분에서는 된장의 것보다 sulfur compound류와 pyrazine류 향기성분의 수와 그 함량이 더 많았다. 순된장의 향기성분 수는 된장의 것보다 많았으나 멸치, 마늘, 고춧가루, 파를 부재료로 첨가하여 가열한 순된장찌개에서는 된장찌개보다 오히려 더 적은 수의 향기성분이 생성되었다 순된장찌개에서 검출된 다양한 pyrazine류는 관능검사의 결과 순된장찌개의 구수한 향에 기여하지 않았다. 된장찌개와 순된장찌개에서 상대적으로 그 함량이 많으며 전체적인 품질의 선호도 및 향기성분 선호도에 관여하는 향기성분은 aldehyde류, alcohol류, ketone류였다.

• 한국식품영양과학회지
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• 제33권5호
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• pp.875-879
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• 2004

생된장에 다시마, 미역 및 멸치를 건조분말상태로 1% 첨가하여 90일간 숙성시킨 후 제품의 품질특성 및 관능특성에 대하여 조사하였다. 일반성분은 수분은 전반적으로 48.7∼50.8%로 큰 차이가 없었고, 숙성 후 저장중에는 해조분말 첨가된장의 수분 유지력이 우수한 것으로 나타났다. 조단백질은 멸치 첨가시 상대적으로 높은 함량을 보였으며, 해조류첨가 시 또는 다시 마와 멸치분말 혼합 첨가시에 탄수화물 함량이 증가됨을 알 수 있었다. 각 된장의 지방 함량은 멸치분말 첨가 된장이 상대적으로 높게 나타났고, 회분은 15% 정도로 거의 차이가 없었다. 각 된장의 pH, 총산도는 미미한 차이를 보였으며 환원당은 다시 마와 멸치분말 혼합 첨가 된장에서 데조구 6.0%에 비하여 9.4%로 매우 높게 나타났다. 아미노산성 질소는 멸치 첨가 된장이 618.7 mg%로 대조구의 531.1 mg%보다 높게 나타났다. 색도와 관능 평가의 경우 밝기 즉 L값으로 인해 기호성이 다르게 나타났는데, 관능적으로 더 밝은 색을 띠는 다시마와 멸치분말 첨가 된장이 대조구나 미역분말 첨가 된장보다 관능적으로 높은 점수를 얻었으며, 전체적인 기호도는 다시마와 멸치분말 첨가 된장의 기호도가 상대적으로 높은 것으로 평가되었다.