• 대한환경공학회지
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• 제34권1호
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• pp.1-6
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• 2012

상수원인 K강 하류부에서의 COD (4~10 mg/L)는 매우 높으며 암모니아성질소의 농도(겨울철 3.5 mg/L) 또한 매우 높다. 암모나아 자체는 이 농도 범위에서 인체에 독성을 주지는 않지만 우리나라 먹는물 기준인 0.5 mg/L로 맞추어져야 한다. 본 연구에서는 K강 상수원을 고도처리 하고자 기존의 일반적인 상수처리공정을 수정하여 파일롯플랜트를 제작하여 운전하였다. 암모니아를 제거하고 염소소독 부산물 일부 제거를 위하여 파괴점 염소주입 및 분말활성탄 투여 공정을 응집조 전 공정에 넣었다. 또한 모래여과 공정 다음에 입상활성탄공정을 넣어 미량 잔류유기물을 제거하고자 하였다. 파일롯플렌트는 36톤/일 규모이며 1년 동안 운전이 되었다. 본 수정된 공정을 통하여 암모니아를 제거하고 여러 유기물질(DOC, MBAS, UV-254 nm absorbance 등)들을 제거할 수 있었다. 유입 DOC 농도는 유입기간 동안 3~6 mg/L 계속 높았으며 1 mg/L로 낮추기 위해서는 GAC 필터의 2 m 높이는 낮은 것으로 판단되었다. 파괴점 염소주입에서 투입 염소농도가 잘 주입이 되었을 때 암모니아의 제거는 98%이상이었으며 낮은 유리 잔류염소 농도와 분말활성탄 투여로 트리할로메탄(THM)은 낮게 검출되었다.

• BMB Reports
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• 제30권1호
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• pp.60-65
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• 1997

Expression of human Cu.Zn-superoxide dismutase (SOD) with activity comparable to human erythrocyte enzyme was achieved in E. coli B21(DE3) by using the pET-17b expression vector containing a T7 promoter. Recombinant human SOD was found in the cytosol of disrupted bacterial cells and represented > 25% of the total bacterial proteins. The protein produced by the E. coli cells was purified using a combination of ammonium sulfate precipitation, Sephacryl S-100 gel filtration and DEAE-Sephacel ion exchange chromatography. The recombinant Cu,Zn-SOD and human erythrocyte enzyme were compared using dismutation activity, SDS-PAGE and immunoblotting analysis. The mass of the subunits was determined to be 15,809 by using a electrospray mass spectrometer. The copper specific chelator. diethyldithiocarbamate (DOC) reacted with the recombinant Cu,Zn-SOD. At $50{\mu}M$ and $100{\mu}M$ concentrations of DOC, the dismutation activity was not inhibited for one hour but gradually reduced after one hour. This result suggests that the reaction of DOC with the enzyme occurred in two distinct phases (phase I and phase II). During phase I of this reaction, one DOC reacted with the copper center, with retention of the dismutation activity while the second DOC displaced the copper, with a loss of activity in phase II.

• 한국환경보건학회지
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• 제24권1호
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• pp.38-46
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• 1998

This study was to investigate the effects of carbon loadings, temperature and expansion ratio on the waterborne organic removal by the biologically active GAC fluidized bed on a laboratory scale. The raw water to be treated comes from midstream of Han river. BACFB(Biological Activated Carbon Fluidized Bed) process was very effective to remove the biodegradable fraction of dissolved organic matter. The more carbon weighed, the more DOC removed in a range from 16.7 to 133.3 g/l. DOC and UV$_{254}$ were removed more than 40% and 20% above 20$\circ$C respectively. Between 5$\circ$C and 10$\circ$C, DOC and UV$_{254}$ were eliminated about 30% and 15% respectively. In general, even if the temperature was higher, DOC removal was a little sensitive, probably influenced by GAC's residual adsorption capacity. UV$_{254}$ reduction was little fluctuated in accordance with water temperature. The gradual increase in expansion ratio from 10% to 75% didn't greatly affect on the removal of DOC and UV$_{254}$. The expansion ratio, therefore, is not a key factor over the critical expansion ratio.

• 한국물환경학회지
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• 제22권1호
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• pp.83-90
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• 2006

Performance and operation of BAC in ozone-BAC advanced water treatment process were investigated using the pilot scale test plant built in D water purification plant. The performance was evaluated by the removal efficiencies of DOC, BDOC, ammonia nitrogen and THMs. The effect of EBCT on DOC removal was experimented for an effective operating condition, and the amount of attached biofilm was analyzed in various water temperatures and position of BAC. Two removal mechanisms, adsorption and biological decomposition by attached biofilm, were predominant to decrease the concentration of various contaminants. DOC was removed 40%, and the removal rate was decreased in winter time due to the lowered activity of attached biofilm. BDOC was effectively removed. THMs and ammonia nitrogen were mainly removed not in ozonation process but in BAC. Water temperature deeply influenced in removal efficiency of ammonia nitrogen. The amount of attached biofilm depended on water temperature and height of packed activated carbon column. Considering DOC removal efficiency and design EBCT of commercial BAC plant, the proper EBCT was 12.5 minutes.

• 한국환경과학회지
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• 제15권1호
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• pp.1-7
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• 2006

The purpose of this study is to illustrate the characteristics of soil organic matter (SOM) and partition coefficient $(K_{DOC})$. Humic substances (HS) from eight soils of varying properties were extracted by two different methods. The dissolved organic carbon (DOC) concentration was stabilized in 22hrs. The ratio of UV absorbance at 465nm and 665nm (E4/E6 ratio) for HS were similar pattern for 8 soils. The extraction with increasing pH increased dissolution of SON. The ratio of organic carbon (OC) associated with HA and FA (the HA:FA ratio) was varied widely in accordance with the soils and was highly correlated to OC $content(\%)$ of the soils. in modeling metal speciation in soils and soil solutions, assumptions that all DOC in soil solution is associated with FA and that HA:FA ratio in SOM is constant have been made. The results of this study indicate that the validity of these assumptions is questionable. By sequential pH extraction, the $K_{DOC}$ showed in a linear correlation with pH.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2018년도 학술발표회
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• pp.487-487
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• 2018

본 연구에서는 2017년 5월부터 12월까지 주단위로 낙동강 수계 5개 보 구간(칠곡보~창녕함안보)에서 본류와 유입지천의 유기물(BOD, $COD_{Mn}$, TOC) 분석 및 상관관계 조사하였으며, 강정고령보~창녕함안보의 대표지점에 대해서는 용존유기물(DOC)의 분해특성을 조사하였다. 본류의 유기물 농도는 BOD $2.7mgO_2/L$($2.3{\sim}3.1mgO_2/L$), $COD_{Mn}$ $5.6mgO_2/L$($5.0{\sim}6.2mgO_2/L$), TOC 4.7 mgC/L($4.2{\sim}5.2mgO_2/L$), 유입지천은 BOD $3.4mgO_2/L$($1.1{\sim}5.6mgO_2/L$), $COD_{Mn}$ $7.1mgO_2/L$($2.5{\sim}10.9mgO_2/L$), TOC $5.7mgO_2/L$(2.3~8.7 mgC/L)로 각각 나타났다. 유기물간 상관성 분석결과 본류의 BOD와 COD, TOC 상관계수(r)은 각각 0.97, 0.98, COD와 TOC는 0.91, 유입지천의 BOD와 COD, TOC는 각각 0.81, 076, COD와 TOC는 0.99로 나타났다. 본류 구간에서 탄소로 환산된 BOD-C/TOC 산화율은 21.5%(20.4~22.5%), COD-C/TOC 45.7%(44.3~48.0%), 유입지천의 BOD-C/TOC 산화율은 23.6%(13.1~31.0%), COD-C/TOC 48.0%(41.3~53.3%)로 각각 나타났다. 강정고령보~창녕함안보 대표지점의 DOC 분해특성은 DOC 중 R-DOC가 86.9%(74.5~94.3%), L-DOC 13.0%(5.7~25.5%)로 나타났다. L-DOC의 분해속도(k)는 0.21 /day(0.11~0.38 /day)로 계산되었으며, 보별 평균 분해속도는 합천창녕보(0.25 /day) > 강정고령보(0.22 /day) > 창녕함안보(0.21 /day) > 달성보(0.18 /day)순으로 나타났다. 유기물 농도는 본류에서는 하류로 갈수록 증가하는 경향을 보였으며, 유입지천은 지점별로 다양하게 나타났다. 유기물간 상관성은 유입지천보다 본류구간이 상관계수가 높게 나타났다. TOC에 대한 BOD와 $COD_{Mn}$산화율은 본류구간 보다 유입지천에서 큰 변동을 보였으며, 실제 유기물 총량 대비 BOD와 $COD_{Mn}$은 각각 77.0%, 52.7% 낮게 나타났다. DOC 중 난분해성 유기물이 대부분을 차지하였으며, 타 보 대비 달성보 내에 난분해성 유기물이 높은 것으로 나타났다.

• 환경위생공학
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• 제13권1호
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• pp.166-173
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• 1998

This study was to investigate the effects of preozonation on the waterborne organic removal by the biologically active GAC fluidized bed on a laboratory scale. The raw water to be treated comes from midstream of Han river. As the ozone dose was increased in the range of $1~3{;\}mgO_{3}/mgDOC$, it produced BDOC from 1.82 to 2.65 times. And BDOC formation can be expressed as [BDOC] = 0.74 + 0.21[DOC] ($R^{2}{\;}={\;}0.8399$). The effects of combination of ozonation followed by BACFB (Biological Activated Carbon Fluidized Bed) process were analyzed. At the ozone doze of $1~3{\;}mgO_{3}/mgDOC$, the large fraction of BDOC was removed by BACFB, but parameter revealed only around 10% reduction.

• 대한환경공학회지
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• 제22권3호
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• pp.453-461
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• 2000

본 연구에서는 XAD-8 수지 추출방법을 이용하여 소양호에 존재하는 수중 부식질의 월별 농도분포를 조사하였고, 추출과정을 통하여 확보한 수중 부식질에 대하여 pH와 탄산이온농도에 따른 오존처리특성을 흡광도와 DOC를 이용하여 검토하였으며, 오존처리한 수중 부식질의 생분해성 및 구조적 변화를 살펴보았다. 1997년 5월에서 10월에 조사된 소양정수장 유입수의 DOC 농도분포는 1~3 mg/L이었고, 수중 부식질의 농도는 0.2~0.8 mg/L로 DOC 농도의 약 20~30%이었다. 수중 부식질 중에서 휴믹산은 약 15~25%, 풀빅산은 약 75~85%로 분포하였다. 오존처리과정의 적정 pH 영역은 오존처리에 따른 부식질의 흡광도 감소율 및 DOC 제거율을 기준으로 판단할 때 pH 7~9 범위이었다. 오존처리과정에서 탄산이온을 첨가하여 라디칼 반응을 억제할 경우 시료의 흡광도 감소율은 증가한 반면 DOC의 제거율은 감소하였다. 오존처리한 시료의 생분해성은 약 50% 이상 증가하였다. $^{13}C-NMR$ 분석결과 오존처리 후 부식질내 방향족화합물의 함량은 49%에서 17%로 감소된 반면, 지방족화합물은 34%에서 51%, 카르복실화합물의 함량은 17%에서 32%로 증가하였다.

• 한국물환경학회지
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• 제23권1호
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• pp.72-80
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• 2007

The seasonal variation of organic matter and the oxidation efficiency of BOD and COD were investigated in several reservoirs and rivers located in the Han River system from March 2005 to April 2006. The concentrations of TOC in the Dong River, the Myeongjicheon stream and the Han-River varied with season. Mean concentration of TOC in the lotic environment ranged from 1.9 to 6.8 mgC/L. Oxidation efficiencies calculated from carbon were approximately 12.6 to 27.1% for BOD and 27.2 to 75.0% for COD, respectively. DOC to TOC ratio in the rivers ranged from 64.3 to 79.0%. Epilimnetic TOC concentrations in two clear and deep lakes (Lake Soyang and Paro) ranged from 2.0 to 2.8 mgC/L. TOC concentrations in the lower three dams (Lake Chunchon, Uiam, and Chungpyung) were relatively high that ranged from 3.4 to 3.7 mgC/L.Oxidation efficiencies in five lakes ranged from 10.6 to 17.2% for BOD and 32.6 to 49.5% for COD, respectively. The range of DOC to TOC in Lake Soyang and Paro was approximately 69. 0 to 73.4%. The ratios of lower three dams ranged from 48.2 to 62.3%. The differences among the lakes seemed to be asociated with limnological and hydrological characteristics of the lakes.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권3호
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• pp.215-222
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• 2006

The Lowry method was used in this study to measure protein in Haemophilus influenzae type b (Hib) conjugate vaccines (polyribosylibitol phosphate-tetanus toxoid; PRP-TT) using deoxycholic acid (DOC) to induce protein precipitation. Trichloroacetic acid (TCA) did not induce precipitation adequately from the Hib conjugate bulk and the freeze-dried Hib conjugate product. Its yield was approximately 50%. The matrix structure of Hib conjugate inhibits precipitation by TCA. Although the Lowry method can be carried out without precipitation in Hib conjugate bulk when no residual impurities (adipic acid dihydrazide [ADH], 1-ethyl-3-(3-dimethylamino-propyl) carbodiimide-HCI [EDAC], phenol and cyanogens bromide [CNBr], etc.) are present, it cannot be used for Hib conjugate products that contain sucrose 8.5%, because 8.5% concentration of sucrose enhanced the protein concentration. DOC- and HCl-induced precipitation is an alternative method for evaluating the protein content of the Hib conjugate bulk and the Hib conjugate product. The precipitation was optimal with a final concentrate of 0.1% for DOC at $4^{\circ}C$ and pH 2. This Lowry method, using DOC/HCI precipitation to induce protein precipitation, was confirmed a consistent, reproducible, and valid test for proteins in Hib conjugate bulk and its freeze-dried product.