• 제목/요약/키워드: Divided cell

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세포융합(細胞融合) 및 Hybridoma 세포작성(細胞作成)에 의한 항자돈백리(抗仔豚白痢) Monoclone항체(抗體)의 생산(生産) (Monoclonal Antibody Production against Piglet Diarrhea Agent (Enterotoxigenic E. coli) by Cell Fusion-Hybridoma Cell Technique)

  • 김우호;안수환;윤용덕
    • 대한수의학회지
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    • 제27권2호
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    • pp.259-267
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    • 1987
  • Enterotoxigenic E. coli (ETEC) cause an acute diarrhea (white scour) in both animals and humans. The disease process initially involves the adherence and colonization of the mucosal surface of the small intestine, followed by the elaboration of a heat-labile enterotoxin (LT) and/or heat-stable enterotoxin (ST). Intestinal adherence or colonization by ETEC is generally mediated by a specific surface-associated pilus (fimbrial) antigen that endows the bacteria with the capacity to adhere to epitherial cell surface. Fourteen monoclonal antibodies (MAbs) directed against pili antigens of ETEC were obtained by cell fusion/hybridoma technique. They were characterized by indirect immunofluorescence assay (IFA), and divided into four groups: specific to K99 antigen (group 1), cross-reactive with K99 and F41 antigens (group 2), specific to K88 antigen (group 3) and specific to 987P and K88 antigens (group 4), respectively. These MAbs demonstrated the distinct pili (K) antigens on the surface of ETEC by IFA, and could be utilized as diagnostic reagent for the identification of ETEC. When eighty-seven field isolates of E. coli from piglet with diarrhea were tested by group 3 MAb, fourty-two strains (48.3%) has K88 pilus antigen suggesting that this is one of the major pilus antigen of ETEC present in fifeld.

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Nd:YAG 레이저빔에 의한 PDP 방전셀의 구조 형성 (Formation of PDP cell structure using Nd:YAG laser beam)

  • 안민영;이경철;이홍규;이천
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2000년도 춘계학술대회 논문집 디스플레이 광소자 분야
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    • pp.129-132
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    • 2000
  • The PDP(Plasma Display Panel) barrier rib material on the glass substrate was patterned for fabrication of the PDP cell using Nd:YAG laser(1064 nm) which can generate the second(532 nm) and forth(266 nm) harmonic wave by HGM(harmonic generation modules). At a scan speed of 20 ${\mu}m/s$ with the second harmonic wave(532 nm) of Nd:YAG laser, the etching threshold laser fluence of the PDP material was 6.5 $mJ/cm^2$ and a sample(thickness = 180 ${\mu}m$) on the glass substrate was removed clearly at a laser fluence of 19.5 $mJ/cm^2$. In order to increase the throughput of the fabrication we divided a single-beam into multi-beams by using a metal mask between the sample and the focusing lens. As a result, 10 lines of PDP cell were formed by one laser beam scanning at a scan speed of 200 ${\mu}m/s$ and a laser fluence of 2.86 $J/cm^2$.

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Porcine Oocytes with Meiotic Competence are Synchronized Early Stage of Germinal Vesicle (GV)

  • Park, Kwang-Wook
    • Reproductive and Developmental Biology
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    • 제34권3호
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    • pp.169-173
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    • 2010
  • Correlations between cumulus cells and germinal vesicle (GV) chromatin configuration were examined in porcine oocytes. Cumulus-oocyte complexes (COCs) were collected from 2~6 mm follicles and divided into three categories according to cumulus cell morphology. "A" group was compacted COCs with more than three cumulus cell layers. "B" group was COCs with less cumulus cell layers than "A" group. "C" group was COCs with one or less layer of cumulus cells. Cumulus cells were removed 0.1% hyaluronidase, and denuded oocytes were stained with Hoechst 33342. GV chromatin configuration was classified into GV-Con and GV-Dis. GV-Con meant that a nucleus was surrounded by condensed chromatin in a ring. GV-Dis meant that filamentous chromatin clumps were distributed in nucleus. The proportion (80.2%) of GV-Con in "A" group was significantly higher than "B" (62.0%) or "C" (44.9%). The proportion (55.1%) of GV-Dis in "C" group was significantly higher than "A" (19.8%) or "B" (38.0%). The meiotic competence of COCs was examined after 44 h culture. The proportion (90.0%) of oocytes reaching to metaphase II (M-II) in "A" group was significantly higher than "B" (76.5%) or "C" (45.5%). In conclusion, oocytes with good quality cumulus cell layers are synchronized early GV stage, and early GV stage is important for meiotic competence in pigs.

신체냉각이 혈액세포성분 및 응혈기전에 미치는 영향에 관한 실험적 연구 (Hematologic and Coagulation Changes in Hypothermic Dogs)

  • 최대영
    • Journal of Chest Surgery
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    • 제2권1호
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    • pp.105-114
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    • 1969
  • This experiment was carried out to study the responses of cellular component of blood and bone marrow to cold and also the changes of coagulation during cooling. Forty-two mongrel dogs were subjected to hypothermia by ice-water surface cooling technique. Lowest body temperature ranged from 21-23 degree. Dogs were divided into 3 groups,Group I, 12 dogs: pentothal anesthesia for 3 hours, Group II, 20 dogs;hypothermic group and Group III,10 dogs;postsplenectomy hypothermic group. Results were summarized as follows: 1. Hemoglobin, hematocrit and red blood cell count significantly increased when animals were cooled, and increase was noted in similar magnitude among the animals of Group I. 2. White blood cell count extremely decreased after cooling and effect of splenectomy on white blood cell count was not apparent. No significant changes were seen among Group I. 3. Differential count of white blood cell when cooled showed relative increase of polymorphonuclear neutrophil and decrease of lymphocyte. 4. There was marked decrease of platelets when body temperature reached to 21-23degree and essentially. no changes was noted in Group I. 5. Clotting time, bleeding time, plasma prothrombin time, recalcification time, and fibrinolysis showed no significant changes when dogs were cooled. Clot retration and prothrombin consumption during hypothermia appeared to be poor. In Group II, bleeding time decreased after splenctomy and when body temperature was lowered, plasma prothrombin time, clot retraction, and prothrombin consumption decreased. Decreased bleeding time and poor clot retraction were noted in Group I. 6. It was found that megacaryocyte count decreased even though platelet count of peripheral blood markedly diminsished when animals were cooled. There was some tendency of erythroid hyperplasia noted during hypothermia.

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마우스에 있어서 에이코사펜타엔산이 면역반응(免疫反應)에 미치는 영향(影響)(II) -II. 세포성(細胞性) 면역(免疫) 및 비특이적(非特異的) 면역(免疫)- (The Effect of Eicosapentaenoic Acid on the Immune Response in Mice(II) -II. Cell-mediated immunity and Nonspecific Immunity-)

  • 안영근;김정훈;이상근;김행순
    • 약학회지
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    • 제33권1호
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    • pp.30-38
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    • 1989
  • The cellular and nonspecific immune response of EPA were investigated in mice. ICR male mice were divided into 8 groups and received intraperiteneal injection of EPA (5 mg, 10 mg, 20 mg/kg) for 4 weeks. Cyclophosphamide (5 mg/kg) was administered i.p. 2days prior to secondary immunization. Immune responses were evaluated by hypersensitivity to SRBC(DTH), rosette forming cell(RFC), natural killer(NK) cell activity and macrophage activity. The obtanined results were as follows: As compared to normal group, 1) DTH was increased by EPA 5 mg, 10 mg administration groups. 2) RFC was significantly increased by EPA 20 mg administration group. 3) NK-Cell activity was significantly increased by EPA 10 mg administration group. 4) Macrophase activity was enhanced by EPA 5 mg administration group.

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$^{60}Co$ 감마선조사가 닭의 정소에 미치는 영향에 관한 연구 (Morphological Study on the Effects of $^{60}Co$ ${\gamma}-irradiation$ on the Testis in the Chicken)

  • 이동명
    • 대한방사선기술학회지:방사선기술과학
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    • 제13권2호
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    • pp.51-51
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    • 1990
  • This study was undertaken to observe the effects of $^{60}Co\;{\gamma}-irradiation$ on the cell of spermatogenic epithelium in the testis of the chicken. 16-week-old chicken were provided as an experimental group and compared with control group. The experimental group was divided into a single irradiation (800, 1000, 1200 rads) and into three partial irradiation group (800/3, 1000/3, 1200/3 rads). The morphological changes of epithelial cell of the testis were observed by means of hematoxyline and eosin stain. Microstructure of spermatocyte and sperm was observed by means of semithin section of electron microscopic specimen. The results obstained are summerized as follows. 1. Spermatogonia and sertoli cells were found to be isolated from the basal membrane of seminiferous tubules as dose of $^{60}Co\;{\gamma}-irradiation$ was increased. 2. Spermatocytes of pachytene stage were seperated from the cytotplasmic process of sertoil cell in case of 1000 rads of $^{60}Co\;{\gamma}-irradiation$. 3. Normal arrangement of the cell of spermatogenic epithelium was found in control group and only the partial irradiation group of 800 rads. Vaculation in the seminiferous was pronounced in case of a single irradiation group of 800 rads, but the irradiation group of 1000 rads and 1200 rads were found to be damaged severely in both a single and a partial dose.

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Clinical Study on Mannan Peptide Combined with TP Regimen in Treating Patients with Non-small Cell Lung Cancer

  • Yan, Huai-An;Shen, Kang;Huang, Xin-En
    • Asian Pacific Journal of Cancer Prevention
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    • 제14권8호
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    • pp.4801-4804
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    • 2013
  • Purpose: To investigate short-term response rate, quality of life and toxicities of mannan peptide combined with TP regimen in treating patients with non-small cell lung cancer (NSCLC). Patients and Methods: Forty one patients with NSCLC were divided into an experimental group treated with TP regimen combined with mannan peptide (21 patients) and a control group treated with TP alone (20 patients). Results: Response rates were 61.9% (13/21) for the experimental and 60% (12/20) for the control group (p>0.05). Regarding toxicity, white blood cell decreased more frequently in the control group (65%, 13/20) than in the experimental group (33.3%, 7/21) (p<0.05); nausea and vomiting also occurred more frequently in the control group (55%, 11/20 vs 23.8%, 5/21) (p<0.05). In terms of quality of life, this index was improved by 57.1% (12/21) and 25% (5/20) in experimental and control groups, respectively (p<0.05). Conclusions: Response rate of TP after combined with mannan peptide is mildly increased, while this combination alleviates bone marrow suppression as well as nausea and vomiting of TP, and improves quality of life when treating patients with NSCLC. However, this conclusion should be confirmed by randomized clinical trails.

FTA 체결에 대비한 임산물 경쟁력 수준과 수출전략품목 분석 (A Study on the Competitive Position of Korean Forest Products and Strategic Exportable Goods)

  • 장우환;권용덕
    • 한국산림과학회지
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    • 제94권1호통권158호
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    • pp.50-57
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    • 2005
  • 이 연구는 일본 중국 아세안과의 FTA체결에 대비하여 우리나라 임산물 시장에서 경쟁력 수준을 파악하고 이를 통해 수출전략품목을 도출하는데 목정이 있다. 이르 위해 1999~2003년간 수입점유율과 수출경쟁력을 이용한 경쟁위치모형을 도출하여 국가간, 품목간 경쟁력 수준을 9가지로 유형화하고 수출전략 품목을 도출하였다. 분석결과, 우리나라의 수출전략 품목은 단기임산물의 경우 송이, 밤, 감, 대추 등이며, 임산물의 경우 섬유판, 마루판, 합판 목재 등이 도출되었다.

계면활성제 유도 건성피부에 대한 Palmarosa, Neroli & Jasmin blending Oil의 유효성 비교 연구 (The Study of Efficiency by Palmarosa, Neroli & Jasmin Essential Oil on Dry Skin Induced by Surfactant)

  • 정현미;최정숙
    • 한국의류산업학회지
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    • 제9권5호
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    • pp.569-572
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    • 2007
  • The effectiveness of Palmarosa, Neroli and Jasmin blending oil on dry skin of rat induced by kitchen detergent are investigated. The experimental groups were divided the control group, group treated with surfactant, group treated with Palmarosa and Neroli, and group with Palmarosa, Neroli and Jasmin. Observation of epidermis and the alteration of mast cell were performed with photomicroscope. According to the epidermis morphological changes analysis, the A3 group treated with Palmarosa, Neroli and Jasmin blending oil was appeared the most similar with the control group, and the A2 group applied with Palmarosa and Neroli blending oil was sequently displayed similar characteristics. The collagen layer's breakaway resulting from Palmarosa essential oil, the collagen layer's restoration resulting from Neroli essential oil, the collagen layer's retention hyperkeratosis resulting from Jasmin essential oil were observed in the structure of the epidermal layer. In photomicrosope observation of mast cell to examine the inflammatory reactions, the increase in size and number of mast cell were showed in A1 group treated with surfactant compared to the control group. The number of mast cells definitely decreased in groups which were treated with Palmarosa, Neroli and Jasmin blending oil.

3GPP LTE 시스템정보(MIB) 추출에 대한 연구 (Design of a 3GPP LTE system Information(MIB) detection simulator)

  • 곽경훈;오혁준
    • 한국정보통신학회:학술대회논문집
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    • 한국정보통신학회 2015년도 추계학술대회
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    • pp.827-829
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    • 2015
  • 본 논문은 LTE(Long Term Evolution)에서 단말이 LTE 망(network)과 통신을 이루기 위해 셀 시스템정보를 획득하는 과정을 연구하였다. 본 논문은 LTE의 셀 시스템정보 두 가지 중 하나인 MIB(Master Information Block)를 추출하는 과정을 다룬다. 3GPP LTE 표준 스펙 시리즈 36.200과 36.300을 연구하여 MATLAB 프로그램으로 MIB를 추출하는 시뮬레이션을 구현하였다. 시뮬레이션 과정은 세 부분으로 나누어져 PSS(Primary Synchronization Signal)와 SSS(Secondary Synchronization Signal)검출, 마지막으로 MIB를 추출하는 부분으로 이루어졌다. PSS와 SSS를 검출하는 과정을 셀 탐색 이라하며, 이 과정은 셀 시스템정보를 추출하기 위해 반드시 이루어져야만 하는 과정이다. 시뮬레이션 프로그램은 실제 Air에서 캡처한 신호를 기반으로 검증하였다.

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