• Title/Summary/Keyword: Divided cell

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An Ellipse Fitting based Algorithm for Separating Overlapping Cells (겹친 세포 분리를 위한 타원 근사 기반 알고리즘)

  • Cho, Mi-Gyung;Shim, Jae-Sool
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2012.05a
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    • pp.909-912
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    • 2012
  • An automated cell tracking system is automatically to analyze and track changes of cell behaviors in time-lapse cell images acquired from microscope in the cell culture. In this paper, we proposed and developed an ellipse fitting based algorithm for separating very small size overlapping cells in a cell image consisted of thousands or ten thousands cells. We were extracted contours of clusters and divided them into line segments and then produced their fitted ellipses for each line segment. By experimentations, our algorithm was separated clusters with average 91% precision for two overlapping cells and average 84% precision for three overlapping cells respectively.

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Cell-Recycled Continuous Ethanol Fermentation of Molasses (당밀의 균체순환식 Ethanol 연속발효)

  • 김익환;김병홍;민태익
    • Microbiology and Biotechnology Letters
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    • v.10 no.2
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    • pp.155-162
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    • 1982
  • A cell-recycled continuous fermentation process was studied to produce ethanol from molasses using Sacchoromyces uvarum ATCC26602 at 35 $^{\circ}C$. The fermentation system was divided into two stages in order to reduce the inhibitions of ethanol and substrate for cell growth and fermentation rate. The first reactor was aerated at the rate of 0.12 vvm whilst the second was kept anaerobic. In medium composition studies, it was revealed that inorganic nutrient supplement to the diluted molasses with 14% fermentable sugar was not needed for the fermentation, however, phosphate limitation was observed when cell propagation was contemplated. By using the cell-recycled continuous fermentation system, 14.5 hour was required to produce 8.4-9.0% (v/v) of ethanol from diluted molasses containing 14% of fermentable sugar. The ethanol productivity was 6.2g/$\ell$hr with the yield of 88.1-94.4% to the theoretical value.

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Study on the Sex-Ratio of Fast- and Slow-Developing Mouse Embryo (Mouse 초기배의 발육속도에 따른 성비에 관한 연구)

  • 이상영;양부근;김정익
    • Korean Journal of Animal Reproduction
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    • v.11 no.3
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    • pp.218-222
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    • 1987
  • This study was conducted using inbred ICR mice to investigate the sex-ratio of preimplantation mouse embryos. For the investigation of sex-ratio of mouse embryos, the karyotype of embryos collected at 70-72, 74-76, 78-80 and 82-84 hr after HCG injection was analyzed by chromosomal analysis. Eight-cell embryos were cultrued up to blastocyst stage, then divided them into three groups(fast-, intermediate- and slow-) according to the blastocoel formation. The sex-ratio was also investigated by chromosomal analysis. 1. The highest apperance of eight-cell and morula was observed at the embryos collected respectively at 66-68 hr(84.6%) and 82-84 hr(79.3%) compared to any other group. 2. The successful rate of embryos sexing at 4-, 8-cell and morula stage were 23.1% (3/13), 42.1%(138/328) and 32.6%(47/141), respectively. The respective sex ratios (female vs male) of 4-, 8-cell and morula were 66.7:33.3, 49.3:50.7 and 39.5:60.5. 3. Of the 476 eight-cell embryos cultured in vitro, 427(89.7%) embryos were developed to the blastocysts and the number of fast-, intermediate- and show-developing embryos were 139, 144 and 144, respectively. 4. Female to male ratios fo fast-, intermediate- and slow-developing group were 23.0:77.0, 55.2:44.8 and 73.8:26.2, respectively. Significantly higher (P<0.05) number of female (48/65;73.8%) was observed in the group of slow-developing embryo than that out of total number of embryos(82/188;43.6%).

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A Study on the Voice Traffic Efficiency and Buffer Management by Priority Control in ATM Multiplexer (ATM 멀티플렉서에서 우선순위 제어에 의한 음성전송효율 및 버퍼관리에 관한 연구)

  • 이동수;최창수;강준길
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.19 no.2
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    • pp.354-363
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    • 1994
  • This paper describes the method that voice traffic is served efficiently in BISDN. Voice is divided into talkspurt and silent period, and it is possible to transmit olny talksurt by the speech activity detection. This paper described the voice traffic control algorithm in the ATM network where cell discarding method is applied to the embedded ADPCM voice data. For traffic control, the cell discarding was used over low priority cells when it overflows the queue threshold. To estimate the efficiency of traffic control algorithm, the computer simuation was performed with cell loss probability, queue length and mean delay as performance parameters. The embedded ADPCM voice coding and cell disscarding resulted in improving the voice cell traffic efficiency and the dynamic control over network congestion.

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Wax Gourd Reduces Adipose Depots and Fat Cell Sizes without Affecting the Levels of Leptin in Rats Fed Medium Fat

  • Kang Keum-Jee
    • Nutritional Sciences
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    • v.9 no.3
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    • pp.173-178
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    • 2006
  • The purpose of this study was to investigate the effects of wax gourd on body weight, body fat, triglyceride, leptin and fat cell size in rats fed medium fat Male Sprague Dawley rats were fed with experimental diet containing total dietary fat at 20% of calorie with wax gourd (WG) for 4 weeks. Forty rats were divided into 4 groups including control, 5, 10, and 15% (w/w) WG supplement The results showed that weight gain was significantly higher in 5% WG than that in control (p<0.05), but was not different among the WG supplement groups. Total fat weight was significantly decreased in both 10 and 15% WG supplement groups than that of control. The levels of triglycerides and free fatty acids in the plasma were significantly decreased, particularly in 15% WG supplement group (p<0.05). The levels of leptin was not significantly different among the experimental groups. Fat cell size was significantly decreased in WG supplement groups compared to that of control group (p<0.05). Correlation among the parameters demonstrated that weight gain correlated positively with total fat weight, the levels of leptin and triglycerides. Taken these results together, 15% WG supplement diet substantially reduces total fat weight, triglycerides and free fatty acids levels and fat cell size in the rat fed midium fat.

Cell-Based Wavelet Compression Method for Volume Data (볼륨 데이터를 위한 셀 기반 웨이브릿 압축 기법)

  • Kim, Tae-Yeong;Sin, Yeong-Gil
    • Journal of KIISE:Computer Systems and Theory
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    • v.26 no.11
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    • pp.1285-1295
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    • 1999
  • 본 논문은 방대한 크기의 볼륨 데이타를 효율적으로 렌더링하기 위한 셀 기반 웨이브릿 압축 방법을 제시한다. 이 방법은 볼륨을 작은 크기의 셀로 나누고, 셀 단위로 웨이브릿 변환을 한 다음 복원 순서에 따른 런-길이(run-length) 인코딩을 수행하여 높은 압축율과 빠른 복원을 제공한다. 또한 최근 복원 정보를 캐쉬 자료 구조에 효율적으로 저장하여 복원 시간을 단축시키고, 에러 임계치의 정규화로 비정규화된 웨이브릿 압축보다 빠른 속도로 정규화된 압축과 같은 고화질의 이미지를 생성하였다. 본 연구의 성능을 평가하기 위하여 {{}} 해상도의 볼륨 데이타를 압축하여 쉬어-? 분해(shear-warp factorization) 알고리즘에 적용한 결과, 손상이 거의 없는 상태로 약 27:1의 압축율이 얻어졌고, 약 3초의 렌더링 시간이 걸렸다.Abstract This paper presents an efficient cell-based wavelet compression method of large volume data. Volume data is divided into individual cell of {{}} voxels, and then wavelet transform is applied to each cell. The transformed cell is run-length encoded according to the reconstruction order resulting in a fairly good compression ratio and fast reconstruction. A cache structure is used to speed up the process of reconstruction and a threshold normalization scheme is presented to produce a higher quality rendered image. We have combined our compression method with shear-warp factorization, which is an accelerated volume rendering algorithm. Experimental results show the space requirement to be about 27:1 and the rendering time to be about 3 seconds for {{}} data sets while preserving the quality of an image as like as using original data.

Chromatin Structural Rearrangement during Dedifferentiation of Protoplasts of Cucumis sativus L.

  • Ondrej, Vladan;Kitner, Miloslav;Dolezalova, Ivana;Nadvornik, Petr;Navratilova, Bozena;Lebeda, Ales
    • Molecules and Cells
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    • v.27 no.4
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    • pp.443-447
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    • 2009
  • This paper reports on the structural rearrangement of satellite DNA type I repeats and heterochromatin during the dedifferentiation and cell cycling of mesophyll protoplasts of cucumber (Cucumis sativus). These repeats were localized in the telomeric heterochromatin of cucumber chromosomes and in the chromocenters of interphase nuclei. The dramatic reduction of heterochromatin involves decondensation of subtelomeric repeats in freshly isolated protoplasts; however, there are not a great many remarkable changes in the expression profile. In spite of that, reformation of the chromocenters, occurring 48 h after protoplast isolation, is accompanied by recondensation of satellite DNA type I; however, only partial reassembly of these repeats was revealed. In this study, FISH and a flow cytometry assay show a correlation between the partial chromocenter and the repeats reassembly, and with the reentry of cultivated protoplasts into the cell cycle and first cell division. After that, divided cells displayed a higher variability in the expression profile than did leaves' mesophyll cells and protoplasts.

Effects of Swainsonine on the Cell-mediated Immune Responses of Lipopolysaccharide (리포포리사카라이드의 세포성 면역반응에 미치는 스와인소닌의 영향)

  • Chae, Byeong-Suk;Ahn, Young-Keun;Kim, Joung-Hoon
    • YAKHAK HOEJI
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    • v.42 no.1
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    • pp.75-81
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    • 1998
  • Effects of swainsonine (SW: 8${\alpha}$, ${\beta}$-indolizidine-1alpha, 2${\alpha}$, 8${\beta}$-triol from Locoweed) on the cellular and nonspecific immune responses of lipopolysaccharide (LPS) wer e studied in ICR mice. Mice were divided into 4 groups (10mice/group), and LPS was given to each mouse 1 hr after i.p. injection with 3.7mg/kg of SW by i.p. injection twice a week for 14 days at a dose of 2mg/kg. Immune responses of the delayed-type hypersensitivity response (DTH) to sheep red blood cells (s-RBC), phagocytic activity and natural killer (NK) cell activity were evaluated. LPS treatment didn`t affect NK cell activity, phagocytic activity, DTH to s-RBC compared with those in controls, and phagocytic activity of sareoma 180 tumor bearing mice. However, circulating leukocytes were significantly decreased. Combinaton of LPS and SW increased circulating leukocytes significantly compared vath that in LPS alone, and DTH to s-RBC, NK cell activity and phagocytic activities of normal and sarcoma tumor bearing mice were not affected. These findings indicate that SW didn`t affected the cellular immune responses suppressed by LPS but significantly increased circulating leukocytes.

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Subjective QoS Performance of MPEG-2 under Cell Loss and Cell Error Conditions (셀손실과 셀오류에 따른 MPEG-2의 주관적 QoS 성능)

  • Han, Jong-Seok;Kim, Yung-Kwon
    • Journal of IKEEE
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    • v.3 no.1 s.4
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    • pp.93-100
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    • 1999
  • When providing MPEG-2 video service through the ATM network, the degradation of QoS caused by cell losses and cell errors occuring from ATM netwok was assessed by the end-users subjectively and then the relation between CLR, CER and subjective QoS of MPEG-2 video was analyzed. Cell losses and cell errors occuring from the ATM network were generated according to the normal and exponential distribution by NEM module. Video sources were divided into sports and drama by moving feature and were tested. The results of our experiments show that the excellent quality of subjective QoS grade 5 was achieved for a cell loss rate of $2{\times}10^{-7}$ and for a cell error rate of $2{\times}10^{-7}$ and the good quality of subjective QoS grade 4 was achieved for a cell loss rate of $2{\times}10^{-6}$ and for a cell error rate of $2{\times}10^{-6}$ When providing MPEC-2 video service through the ATM network, the results of our experiments will be used as valuable information to determine the network performance objectives for satisfying the QoS required by the end-users and to design the network.

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THE DENTAL FACTORS IN ORAL SQUAMOUS CELL CARCINOMA (구강편평상피세포암에서의 치성요인)

  • Nam, Woong;Cha, In-Ho
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.27 no.6
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    • pp.519-525
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    • 2001
  • The oral cavity has frequent contacts with many carcinogenic compounds and its soft tissue is continuously stimulated by numerous dental factors. We have examined the detailed dental factors and its correlation with oral squamous cell carcinoma, and denture-wearing effects to analyze the effect of the dental factors on the genesis of oral squamous cell carcinoma. We have studied clinical contributing factors and the dental factors in the genesis of oral squamous cell carcinoma when the effects of smoking and drinking are controlled. The study cases are 100 patients(75 males and 25 females) who were diagnosed histo-pathologically as squamous cell carcinoma at the Yonsei Medical Center. The control group was 154 patients who have no systemic malignant tumors. The effects of 6 dental factors were analyzed in this study. They were divided into the smoking group, the non smoking group, the drinking group, and the non-smoking group. The effects of dental factors were analyzed in each group. In this study, we have drawn some conclusions on the relationship between the dental factors and oral squamous cell carcinoma using $x^2$-test. 1. The repaired teeth have statistical significance on the genesis of squamous cell carcinoma. This is probably due to the combining effects of past poor oral hygiene and continuous stimulation due to poor prosthesis. 2. There is statistical significance of the lost teeth in the smoking group, and the repaired teeth and the degree of alveolar bone resorption had statistical significance in the non-smoking group. 3. Smoking and drinking by-itself have no statistical significance in the genesis of oral squamous cell carcinoma. However, in combination, they have statistical significance. In this study, dental factors had a synergistic effect with smoking and drinking. Together with avoidance of smoking and drinking, appropriate restoration and oral hygiene control are most important factors in the preventive aspects of the oral squamous cell carcinoma.

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