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Surface ultrastructure of Parvatrema timondavidi (Digenea: Gymnophallidae) according to its developmental stages (Parvatrema timondavidi (Digenea: Gymnophallidae) 피낭유충, 유약충 및 성충의 표피 미세구조)

  • Yu, Jae-Ran;Park, Jin-Yeong;Chae, Jong-Il
    • Parasites, Hosts and Diseases
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    • v.32 no.2
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    • pp.65-74
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    • 1994
  • Surface ultrastructure of Pawctrema timonnauini developmental stages was studied using a scanning electron microscope. The metacercariae were collected from the marine clam, Topes phiLippinam and juvenile and adult worms were recovered at 1, 2, 3 and 7 days after experimental infection of mice. The metacercariae had a large oral sucker and characteristic lateral projections. Around the lip of the oral sucker many type I and type II sensory papillae were observed, and type III papillae were located symmetrically on the medial side of the lateral projection. Numerous type I papillae were grouped around the genital pore. The tegumental spines were distributed over the worm surface except the lip of the suckers and genital pore. The 1-day old worm had a well-developed ventral sucker, with 6 type II sensory papillae on its outer surface and another 6 type I papillae on the inner side. Two small type I papillae were seen on the anterior side of the ventral sucker. The genital pore was small and opened separately from the ventral sucker and 15 type I papillae were grouped around it. The 2-, 3-. and 7-day worms revealed that as they grew to be adults, the spine tips became multipointed, the genital pore formed a genital atrium, and the cytoplasmic process became well differentiated. In 2- and 3-day worms 10 type II papillae encircling the lip of the oral sucker, and additional 4 papillae at the dorsal side of 4 dorsal type II papillae were a characteristic feature. The distribution pattern of sensory papillae around the oral sucker and genital pore, and 2 type I papillae on the anterior side of the ventral sucker, was so peculiar in R timonnnuini, that they seem to be useful keys for taxonomic differentiation from other gymnophallids.

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The Role of Adipokines in the Pathogenesis of Non-alcoholic Fatty Liver Disease in Obese Children; the Relationship between Body Fat Distribution and Insulin Resistance (비만아의 비알코올성 지방간 발병에 있어 Adipokine과 체지방분포 및 인슐린 저항성과의 연관성에 대한 연구)

  • Yang, Hye-Ran;Ko, Jae-Sung;Seo, Jeong-Kee
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.10 no.2
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    • pp.185-192
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    • 2007
  • Purpose: This study was conducted to evaluate the role of adiponectin, leptin, and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) in the pathogenesis of non-alcoholic fatty liver disease (NAFLD) in obese children, and to elucidatethe relationship between these adipokines and insulin resistance. Methods: A total of 61 obese children (M : F=42 : 19, mean age 11.2${\pm}$1.3 years) admitted to our facility between March 2004 and June 2005 were included in this study. Patients were divided into three groups based on their NAFLD status obese children without fatty liver (N=23); obese children with simple steatosis (N=20); and obese children with non-alcoholic steatohepatitis (NASH) (N=18). The serum levels of adiponectin, leptin, and TNF-${\alpha}$ were measured, and insulin resistance determined by homeostasis model assessment (HOMA-IR) was calculated to estimate insulin resistance. In addition, the VSR (visceralsubcutaneous fat ratio) was estimated using abdominal computed tomography. Results: There was no difference in serum TNF-${\alpha}$ and leptin levels observed between the 3 groups (22.13${\pm}$6.37 vs. 21.35${\pm}$6.95 vs. 25.17${\pm}$9.30; p=0.342 & 20.29${\pm}$8.57 vs. 16.42${\pm}$6.85 vs. 20.10${\pm}$7.86; p=0.330). However, the serum adiponectin level was significantly lower in children with non-alcoholic steatohepatitis (NASH) than in the other two groups (6.08${\pm}$1.38 in children without steatosis vs. 5.69${\pm}$0.79 in simple steatosis vs. 4.93${\pm}$1.75 in NASH; p=0.026). In addition, the VSR was significantly increased in the NASH group (0.31${\pm}$0.08 vs. 0.32${\pm}$0.11 vs. 0.47${\pm}$0.14; p=0.001), and HOMA-IR revealed a significant difference among the three groups (4.77${\pm}$3.67 vs. 6.89${\pm}$7.05 vs. 10.42${\pm}$6.73; p=0.000). However, there was no significant correlation observed between the adiponectin levels and the HOMA-IR or the VSR (r=-0.117; p=0.450 & r=-0.106; p=0.499). Conclusion: Insulin resistance may affect the development of hepatic steatosis and steatohepatitis in children, and the results of this study suggest that, of several adipokines evaluated, adiponectin is important in the progression of steatosis to steatohepatitis in obese children.

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Biogeochemical Studies on Tidal Flats in the Kyunggi Bay: Introduction (경기만 부근 갯벌의 생지화학적 연구: 서문)

  • Cho, B.C.;Choi, J.K.;Lee, T.S.;An, S.;Hyun, J.H.
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.10 no.1
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    • pp.1-7
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    • 2005
  • Tidal flats have been regarded to carry out transformation and removal of land-derived organic matter, and this purifying capability of organic matter by tidal flats is one of very important reasons for their conservation. However, integral biogeochemical studies on production and decomposition of organic matter by benthic microbes in tidal flats have been absent in Korea, although the information is indispensable to quantification of the purifying capability. Our major goals in this multidisciplinary research were to understand major biogeochemical processes and rates mediated by diverse groups of microbes dominating material cycles in the tidal flats, and to assess the contribution of benthic microbes to removal of organic matter and nutrients in the tidal flats. Our study sites were Ganghwa and Incheon north-port tidal flats that had been regarded as naturally well reserved and organically polluted, respectively. Our research group measured over 3 years primary production, biomass and community structure of primary producers, abundance and production of bacteria, enzyme activities, distribution of protozoa and protozoan grazing rates, rates of denitrification and sulfate reduction, early sediment diagenesis, primary production and respiration based on oxygen microelectrode. We analyzed major features of each biogeochemical process and their interactions. The results are compiled in the following articles in this special issue: An (2005), Hwang and Cho (2005), Mok et at. (2005), Na and Lee (2005), Yang et at. (2005), and Yoo and Choi (2005).

A New Method For Measuring Acupoint Pigmentation After Cupping Using Cross Polarization (교차편광 촬영술(Cross Polarization Photographic Technique)를 이용한 부항요법의 배수혈 피부 색소 침착 변화 측정 평가)

  • Kim, Soo-Byeong;Jung, Byungjo;Shin, Tae-Min;Lee, Yong-Heum
    • Korean Journal of Acupuncture
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    • v.30 no.4
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    • pp.252-263
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    • 2013
  • Objectives : Skin color deformation by cupping has been widely used as a diagnostic parameter in Traditional Korean Medicine(TKM). Skin color deformation such as ecchymoses and purpura is induced by local vacuum in a suction cup. Since existing studies have relied on a visual diagnostic method, there is a need to use the quantitative measurement method. Methods : We conducted an analysis of cross-polarization photographic images to assess the changes in skin color deformation. The skin color variation was analyzed using $L^*a^*b^*$ space and the skin erythema index(E.I.). The meridian theory in TKM indicates that the condition of primary internal organs is closely related to the skin color deformation at special acupoints. Before conducting these studies, it is necessary to evaluate whether or not skin color deformation is influenced by muscle condition. Hence, we applied cupping at BL13, BL15, BL18, BL20 and BL23 at Bladder Meridian(BL) and measured blood lactate at every acupoint. Results : We confirmed the high system measurement accuracy, and observed the diverse skin color deformations. Moreover, we confirmed that the $L^*$, $a^*$ and E.I. had not changed after 40 minutes(p>0.05). The distribution of blood lactate levels at each part was observed differently. Blood lactate level and skin color deformation at each part was independent of each other. Conclusions : The negative pressure produced by the suction cup induces a reduction in the volumetric fraction of melanosomes and subsequent reduction in epidermal thickness. The relationship between variations of tissue and skin properties and skin color deformation degree must be investigated prior to considering the relationship between internal organ dysfunction and skin color deformation.

Development of an Emergence Model for Overwintering Eggs of Metcalfa pruinosa (Hemiptera: Flatidae) (미국선녀벌레(Metcalfa pruinosa) (Hemiptera: Flatidae) 월동난 부화 예측 모델 개발)

  • Lee, Wonhoon;Park, Chang-Gyu;Seo, Bo Yoon;Lee, Sang-Ku
    • Korean journal of applied entomology
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    • v.55 no.1
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    • pp.35-43
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    • 2016
  • The temperature-dependent development of Metcalfa pruinosa overwintering eggs was investigated at ten constant temperatures (12.5, 15, 17.5, 20, 22.5, 25, 27.5, 30, 32.5, and $35{\pm}1^{\circ}C$, Relative Humidity 20~30%). All individuals collected before April 13, 2012 failed to develop into first instar larvae. In contrast, some individuals that were collected on April 11, 2013 successfully developed when reared under $20{\sim}32.5^{\circ}C$ temperature regimes. The developmental duration was shortest at $30^{\circ}C$ (13.3 days) and longest at $15^{\circ}C$ (49.6 days) in the fourth collected colony (April 26 2013). Developmental duration decreased with increasing temperature up to $30^{\circ}C$ and development was retarded at high-temperature regimes ($32.5^{\circ}C$). The lower developmental threshold was $10.1^{\circ}C$ and the thermal constant required to complete egg overwintering was 252DD. The Lactin 2 model provided the best statistical description of the relationship between temperature and the developmental rate of M. pruinosa overwintering eggs ($r^2=0.99$). The distribution of the developmental completion of overwintering eggs was well described by the 2-parameter Weibull function ($r^2=0.92$) based on the standardized development duration. However, the estimated cumulative 50% spring emergence dates of overwintering eggs were best predicted by poikilotherm rate model combined with the 2-parameter Weibull model (average difference of 1.7days between observed and estimated dates).

Evaluation for Soil Moisture Stabilization and Plant Growth Response in Horizontal Biofiltration System Depending on Wind Speed and Initial Soil Moisture (풍속과 초기 토양수분에 따른 평면형 바이오필터 내 토양수분 안정화 및 식물 생육반응 평가)

  • Choi, Bom;Chun, Man Young;Lee, Chang Hee
    • Korean Journal of Plant Resources
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    • v.27 no.5
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    • pp.546-555
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    • 2014
  • The final aim of this study is to develop a biofiltration system integrated with plant vegetation for improving indoor air quality effectively depending on indoor space and characteristics. However, to approach this final goal, several requirements such as constant pressure drops (PDs) and soil moisture contents (SMCs), which influence the capacity design for a proper ventilation rate of biofiltration system, should be satisfied. Thus, this fundamental experiment was carried out to adjust a proper wind speed and to ensure a stabilization of initial SMCs within biofilter for uniform distribution of SMCs and PDs, and for normal plant growth, especially avoiding root stress by wind. Therefore, we designed horizontal biofliter models and manufactured them, and then calculated the ventilation rate, air residence time, and air-liquid ration based on the biofilter depending on three levels of wind speed (1, 2, and $3cm{\cdot}s^{-1}$). The relative humidity (RH) and PD of the humidified air coming out through the soil within the biofilter, and SMC of the soil and plant growth parameters of lettuce and duffy fern grown within biofilter were measured depending on the three levels of wind speed. As a result of wind speed test, $3{\cdot}sec^{-1}$ was suitable to keep up a proper RH, SMC, and plant growth. Thus, the next experiment was set up to be two levels of initial SMCs (low and high initial SMC, 18.5 and 28.7%) within each biofilter operated and a non-biofiltered control (initial SMC, 29.7%) on the same wind speed ($3cm{\cdot}sec^{-1}$), and measured on the RH and PD of the air coming out through the soil within the biofilter, and SMC of the soil and plant growth parameters of Humata tyermani grown within biofilter. This result was similar to the first results on RHs, SMCs, and PDs keeping up with constant levels, and three SMCs did not show any significant difference on plant growth parameters. However, two biofiltered SMCs enhanced dry weights of the plants slightly than non-biofiltered SMC. Thus, the stability of this biofiler system keeping up major physical factors (SMC and PD) deserved to be adopted for designing an advanced integrated biofilter model in the near future.

A Nested Cauldron Structure in the Tertiary Miocene Eoil Basin, Southeastern Korea (한반도 동남부 제3기 마이오세 어일분지내 둥지형 화산함몰구조)

  • Son, Moon;Kim, In-Soo;Ock, Soo-Seok
    • The Journal of the Petrological Society of Korea
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    • v.10 no.2
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    • pp.121-131
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    • 2001
  • The combination of geological, structural and satellite image studies is used to make an examination of the Miocene eruptive type in the Eoil Basin, SE Korea. The basin subsided by the NW-SE extension due to NNW dextral shearing during the East Sea opening. Based on geological structures as well as lithofacies and ages of the basin-fills, it is divided into the NE subbasin and the SW subbasin which were abundantly filled with basaltic volcanics and marine sediments without volcanic materials, respectively: Syndeposional synclines and anticlines are characteristically developed in the NE subbasin, which amplitudes decrease away from the adjacent normal faults to make them into a homoclinal structure. The thicker lavas as well as the younger agglomerates and lacustrine sediments, which show circular distributions, are distributed around the axial zones of major synclines. The satellite image shows four remarkable circular structures within the NE subbasin. They are located adjacent to and along the normal faults, and they are laid almost exactly on the axial zones of the synclines as well as on the distribution area of the agglomerates and lacustrine sediments. These facts indicate that the basaltic lava effusion were conducted by the normal faults like a kind of fissure-eruption and its activity was more predominant at the sites in where the synclines are developed. More active effusion of lava became a reason for deeper subsidence to make differential subsidence and syndepositional folding adjacent to and along the normal faults. Hence, we suggest that a nested cauldron structure was formed in the NE subbasin of the Eoil Basin, and that the volcanism made the subbasin to be a lava pond and controlled the process of filling and sedimentation in the subbasin.

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Evaluation of Hydrogeologic Seal Capacity of Mudstone in the Yeongil Group, Pohang Basin, Korea: Focusing on Mercury Intrusion Capillary Pressure Analysis (포항분지 영일층군 이암층의 수리지질학적 차폐능 평가: 수은 모세관 압입 시험의 결과 분석을 중심으로)

  • Kim, Seon-Ok;Wang, Sookyun;Lee, Minhee
    • Economic and Environmental Geology
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    • v.53 no.1
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    • pp.23-32
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    • 2020
  • Geological CO2 sequestration is a global warming response technology to limit atmospheric emissions by injecting CO2 captured on a large scale into deep geological formations. The presented results concern mineralogical and hydrogeological investigations (FE-SEM, XRD, XRF, and MICP) of mudstone samples from drilling cores of the Pohang basin, which is the research area for the first demonstration-scale CO2 storage project in Korea. They aim to identify the mineral properties of the mudstone constituting the caprock and to quantitatively evaluate the hydrogeologic sealing capacity that directly affects the stability and reliability of geological CO2 storage. Mineralogical analysis showed that the mudstone samples are mainly composed of quartz, K-feldspar, plagioclase and a small amount of pyrite, calcite, clay minerals, etc. Mercury intrusion capillary pressure analysis also showed that the samples generally had uniform particle configurations and pore distribution and there was no distinct correlation between the estimated porosity and air permeability. The allowable CO2 column heights based on the estimated pore-entry pressures and breakthrough pressures were found to be significantly higher than the thickness of the targeting CO2 injection layer. These results showed that the mudstone layers in the Yeongil group, Pohang basin, Korea have sufficient sealing capacity to suppress the leakage of CO2 injected during the demonstration-scale CO2 storage project. It should be noticed, however, that the applicability of results and analyses in this study is limited by the lack of available samples. For rigorous assessment of the sealing efficiency for geological CO2 storage operations, significant efforts on collection and multi-aspect evaluation for core samples over entire caprock formations should be accompanied.

Seagrass (Zostera marina L., Zosteraceae) Bed in the Brackish Lake Hwajinpo, Korea (화진포 기수호에 해산식물 거머리말 (Zostera marina L., Zosteraceae))

  • Lee, Sang-Yong;Kwon, Chun-Joong;Heo, Sung;Choi, Chung-Il
    • Korean Journal of Ecology and Environment
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    • v.33 no.4 s.92
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    • pp.336-341
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    • 2000
  • This study was conducted to clarify the habitat characteristics and distribution of seagrass. Zostera marina L. (Zosteraceae) in the brackish Hwajinpo Lake, Korea in June 1998 and July 2000. Z. marina beds were distributed along the sea-side cost of the lower lake mouth at 0.8 to 1.5m in death, and the seagrass bed area was about 3,200 m$^{2}$. Salinity, water temperature and pH were in the range of 8.0${\sim}$23.0$%_{o}$, 22.0${\sim}$23.7$^{\circ}C$ and 8.34${\sim}$8.62, respectively. Nutrient concentrations were generally now (TN: 24.34 ${\mu}$M, NH$_{4}$-N: 2.57 ${\mu}$M, NO$_{3}$-N: 0.56 ${\mu}$M, NO$_{2}$-N: 0.27 ${\mu}$M, TP: 2.08 ${\mu}$M, PO$_{4}$-P: 0.34 ${\mu}$M). Suspended particulate matters (SPM) concentration averaged 62.8 mg/l and particulate organic matter (POM) averaged 21.3 mg/l. Organic content of SPM averaged 33.9%. The beds substratum was composed of well-sorted, fine sand and its mean brain size was 3.13${\Phi}$. The Z. marina vegetation was almost submerged, and the morphological characteristics can be classified as steno-leaf phenotype by the shoot length, leaf width, and number of leaf vein. Shoot length and leaf width were 70.0${\sim}$126.5 cm and 5${\sim}$7 mm, respectively. Shoot densities ranged from 264 to 296/m$^{2}$, and the plants biomass was estimated at 332.6 to 373.0 g dw/m$^{2}$. Therefore, the habitats of Z. marina in Korea were recognized in a brackish lake, and morphological characteristics appeared to be variable.

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Assessment of the Potential Consumers' Preference for the V2G System (V2G 시스템에 대한 잠재적 소비자의 선호 평가)

  • Lim, Seul-Ye;Kim, Hee-Hoon;Yoo, Seung-Hoon
    • Journal of Energy Engineering
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    • v.25 no.4
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    • pp.93-102
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    • 2016
  • Vehicle-to-Grid (V2G) system, bi-direction power trading technology, enables drivers possessing electric vehicle to sell the spare electricity charged in the vehicle to power distribution company. The drivers gain profit by charging electricity in the day time of high electricity rate. In this regard, the government is preparing the policies of building and supporting V2G infrastructure and demanding the potential consumers' preference for the V2G system. This paper attempts to analyze the consumers' preference using the data from obtained a survey of randomly selected 1,000 individuals. To this end, choice experiment, an economic technique, is employed here. The attributes considered in the study are residual amount of electricity, electricity trading hours, required plug-in time, and price measured as an amount additional to current gasoline vehicle price. The multinomial logit model, which requires the assumption of 'independence of irrelevant alternatives', is applied but the assumption could not be satisfied in our data. Thus, we finally utilized nested logit model which does not require the assumption. All the parameter estimates in the utility function are statistically significant at the 10% level. The estimation results show that the marginal willingness to pay (MWTP) for one hour increase in electricity trading hours is estimated to be KRW 1,601,057. On the other hand, a one percent reduction in residual amount of electricity and one hour reduction in required plug-in time in V2G system are computed to be KRW -91,911 and -470,619, respectively. The findings can provide policy makers with useful information for decision-making about introducing and managing V2G system.