• Korean Journal of Ecology and Environment
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• v.44 no.3
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• pp.292-302
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• 2011

The Lake Sihwa watershed includes industrial, urban, and rural areas simultaneously. In this study, we analyzed the characteristics of dissolved organic matter (DOM) in spatial-temporal runoff patterns at representative sites having different land use in the watershed of Lake Sihwa. The result of synchronous and 3D-EEMs(3-Dimensional Excitation Emission Matrix Spectroscopy) analysis in 4TG (industrial area), fluorescence distribution and variation clearly appeared in the Fulvic-like fluorescence (FLF) and Humic-like fluorescence (HLF) regions along with the Protein-like fluorescence (PLF) region. A characteristic that Peak A (HLF) region fluorescence intensity did not decrease and the HLF region of fluorescence intensity and spatial-temporal changes clearly appeared during rainfall in AS (urban area). The results of fluorescence analysis in MS did not show great changes in PLF and FLF while showing that fluorescence intensity changes over time in the Terrestrial-like fluorescence (THLF) region increased greatly. In conclusion, our results showed significant differences in the runoff characteristics of DOM particularly in industrial, urban and rural area, and these differences should be considered for the efficient controlling of DOM in the watershed.

• The Journal of the Korean Society for Microbiology
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• v.15 no.1
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• pp.47-54
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• 1980

Exoenzymes, protease(P) and elastase(E) produced by Pseudomonas aeruginosa are reported to have close relationship with pathogenicity of Pseudomonas aeruginosa. Productibility of exoenzymes P and E were studied and compared in environmental isolates from hospital environments and clinical isolates from various clinical specimens, also, the relationship between their enzyme production and serotype were reviewed. 1. Clinical isolates were typed into nine serotypes A, B, C, D, E, F, G, H and I. Serotype E had the highest incidence of 24%, followed by B with 16.8%, G, 15.1% and C, 9.3%. 2. Environmental isolates were, typed as serotype B, C, E, F, G, H, I, K and M. Serotype I had the highest incidence of 26.6%, followed by C, F and M each incidence of 14.3%. 3. In the typing of the above two groups, serotypes A and D were found only in the clinical isolates and serotypes K, and M were found only in the environmental isolates. Serotypes J and L were found in neither clinical isolates nor environmental isolates. 4. In the distribution of serotypes from various clinical specimens, serotype G among isolates from pus showed incidence of 20.4%, and serotypes E and B were 19.5% separately. Serotype E had incidence of 22.6% and 20.0% in urine and sputa respectively, showing a high rate compared to the other serotypes. 5. The incidence of strains producing both exoenzymes P and E was 77.8% in the preserved strains of clinical isolates and 76.2% in the environmental isolates. There were no significant difference between the two groups. 6. Serotypes A and H, which are preserved strains from clinical isolates showed productibility of both exoenzymes P and E, the other serotypes showed productibility of various combination of exoenzymes. Among the environmental isoaltes, production of both exoenzymes P and E were seen in serotypes E, F, G, H, I and K and no serotype produced only P or E. 7. In ability to produce exoenzymes of isolates from sources of various clinical specimens, strains producing both exoenzymes P and E were found most frequently in pus with incidence rate of 82.0%, followed by 80.0% in sputum and urine. 8. Almost all the fresh strains of clinical isolates were producers of both exoenzymes P and E.

• Journal of fish pathology
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• v.10 no.1
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• pp.21-29
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• 1997

We investigated on the prevalence and extermination of scuticociliatids parasitic on cultured japanese flounder, Paralichthys olivaceus in land-marine tank system of southern Korea from January to February in 1997. The gills and the skin showed the highest infection rate(60%), and the brain showed the lowest(22%). Also, fish secreted large quantity of mucus with a bleeding and ulcerated lesions on the infected sites. The number of the parasites in inflowing sea water, surface water and bottom water of farming tank ranged 0~1 individuals/$100m\ell$, 0~413 individuals/$100m\ell$ and $7\sim7.3{\times}10^4$ individuals/$100m\ell$, respectively. This parasite was died within 2 hours in 50~500 ppm, 48 hours of 10 ppm formalin or hydrogen peroxide, 1 hour in 50~500 ppm, 80 minutes of 10 ppm oligo chitosan and 10 minute in 100% but did not died until 48 hours in 10~70% fresh water.

• Journal of fish pathology
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• v.20 no.3
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• pp.229-235
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• 2007

Cell number of Vibrio spp. was examined for the surface and bottom sea water harvested from the 9 stations in the Jeonam Archipelago Marine Ranching Ground in 2006～2007. Species population composition and dominant species were analyzed as well. The largest number of Vibrio spp. was detected in summer (June, 2007) among the 4 seasons with the surface and bottom sea water samples of 5.4～93.2 cfu㎖-1 and 1.0～53.1 cfu㎖-1. respectively. In winter (January, 2007), Vibrio spp. was not detected at all except that one station from which 0.2 cfu㎖-1 of Vibrio spp. was counted in the surface sea water sample. Cell numbers were 0～11.9 cfu㎖-1 and 0～8.3 cfu㎖-1, respectively, in the surface and bottom sea water samples retrieved in spring (April, 2007), and the corresponding sea water samples in autumn(November, 2006) were contaminated with 0.1～21.4 cfu㎖-1 and 0～2.9 cfu㎖-1, respectively. Species population composition of Vibrio spp. was found to be V. parahaemolyticus, V. alginolyticus and V. cholerae non-01 among which V. parahaemolyticus and V. alginolyticus were dominant in all seasons. V. alginolyticus was detected from all stations even in the winter season indicating that this species seems to be less temperature dependent than the other Vibrio spp..

• Fire Science and Engineering
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• v.28 no.4
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• pp.35-43
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• 2014

The prediction performance of 9 model sets, which combine 3 turbulent models and 3 combustion models, was investigated numerically for turbulent partially-premixed jet flame. The standard ${\kappa}-{\varepsilon}$ (SKE), Realizable ${\kappa}-{\varepsilon}$ (RKE) and Reynolds stress model (RSM) were used as a turbulence model, and the eddy dissipation concept (EDC), steady laminar flamelet (SLF) and unsteady laminar flamelet model (ULF) were also adopted as a combustion model. The prediction performance of those 9 model sets was evaluated quantitatively and qualitatively for Sandia D flame of which flame structure was measured precisely. The flame length was predicted as, from longest to shortest, RSM > SKE > RKE, and the RKE predicted the flame length of the jet flame much shorter than experiment. The flame temperature was over predicted by the combination of RSM + SLF or RSM + ULF while the flame length obtained by RSM + SLF and RSM + ULF was well agreed with the experiment. The combination of SKE + SLF and SKE + ULF predicts well the flame length as well as the temperature distribution. The SKE turbulence model was most superior to the other turbulent models, and SKE + ULF showed the best prediction performance for the structure of turbulent partially-premixed jet flame.

• Korean Journal of Food Science and Technology
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• v.25 no.2
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• pp.165-170
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• 1993

The average concentration of Pb in 53 samples of canned orange juice currently sold in Korean market was 0.225 mg/kg, and that of Sn, 40.7 mg/kg. There was no appreciable difference in Pb concentration according to elapsed time after manufacturing, whereas Sn concentration increased 0.66 mg/kg per month. During the storage at room temperature or in refrigerator after opening, the Pb concentration increased slowly, reaching 1.7 to 1.8 times of original concentration, whereas Sn concentration increased by 20% per day, resulting in 3 times of original concentration after 7 days. There were no serious changes in Pb and Sn concentration in storage at room temperature or refrigerator for 3 days, when juice samples were opened and transferred to glass container. It is needed that detailed inspection by undertaken to monitor the contents of heavy metals in canned orange juice, since 18% of samples within recommended distribution period exceeded the legal standard for Pb, and recommended that more attention be paid in handling canned orange juice after opening, in order to avoid the hazard from heavy metals.

• Korean Journal of Food Science and Technology
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• v.6 no.2
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• pp.75-78
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• 1974

An experiment has been carried out in order to analyze the main components of Korean Cattles' milk, and fractionate the milk protein by DEAE-cellulose column. The results obtained were summarized as follow. 1) The average values of specific gravity, pH and acidity of Korean Cattles milk which were negative in alcohol test were 1,036, 6.4 and 0.21, respectively. 2) The average values of total solids, solids-not-fat, protein, lactose and ash contents of Korean Cattles milk were 11.61%, 9.53%, 2.08%, 3.99%, 4.76% and 0.86%, respectively. 3) Distribution of casein, whey protein, N.P.N., protein precipitated in 12% TCA, lactoglobulin and lactalbumin contents of the milk were 3.07%, 1.13%, 0.10%, 4.06%, 0.34% and 0.66%, respectively. 4) Acid casein obtained from Korean Cattles milk was fractionated into four fractions on DEAE-cellulose column with 0.005M tris-citrate buffer containing 6M urea, pH 8.6, and the ratio of the fraction I, II, III and IV was 3.24%, 52.67%, 26.22% and 17.87%, respectively. 5) Whey protein obtained from Korean Cattles milk was also fractionated into four fractions on DEAE-cellulose column with 0.04M phosphate buffer, pH 5.8, and the ratio of the fraction I, II, III and IV was 41.74%, 10.17%, 1.50% and 46.59%, respectively.

• Journal of Pharmacopuncture
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• v.19 no.4
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• pp.319-328
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• 2016

Objectives: Advancements in nanotechnology have led to nanoparticle (NP) use in various fields of medicine. Although the potential of NPs is promising, the lack of documented evidence on the toxicological effects of NPs is concerning. A few studies have documented that homeopathy uses NPs. Unfortunately, very few sound scientific studies have explored the toxic effects of homeopathic drugs. Citing this lack of high-quality scientific evidence, regulatory agencies have been reluctant to endorse homeopathic treatment as an alternative or adjunct treatment. This study aimed to enhance our insight into the impact of commercially-available homeopathic drugs, to study the presence of NPs in those drugs and any deleterious effects they might have, and to determine the distribution pattern of NPs in zebrafish embryos (Danio rerio). Methods: Homeopathic dilutions were studied using high-resolution transmission electron microscopy with selected area electron diffraction (SAED). For the toxicity assessment on Zebrafish, embryos were exposed to a test solution from 4 - 6 hours post-fertilization, and embryos/larvae were assessed up to 5 days post-fertilization (dpf ) for viability and morphology. Toxicity was recorded in terms of mortality, hatching delay, phenotypic defects and metal accumulation. Around 5 dpf was found to be the optimum developmental stage for evaluation. Results: The present study aimed to conclusively prove the presence of NPs in all high dilutions of homeopathic drugs. Embryonic zebrafish were exposed to three homeopathic drugs with two potencies (30CH, 200CH) during early embryogenesis. The resulting morphological and cellular responses were observed. Exposure to these potencies produced no visibly significant malformations, pericardial edema, and mortality and no necrotic and apoptotic cellular death. Conclusion: Our findings clearly demonstrate that no toxic effects were observed for these three homeopathic drugs at the potencies and exposure times used in this study. The embryonic zebrafish model is recommended as a well-established method for rapidly assessing the toxicity of homeopathic drugs.

• 한국해양학회지
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• v.25 no.2
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• pp.106-116
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• 1990

The dynamics and seasonal succession of dinoflagellate community, and their correlations with environmental parameters were investigated during the period from April, 1986 to March, 1987 at 6 selected stations in Masan Bay, Korea. The dinoflagellate standing crops varied extensively with months and stations: ranging from 44 to 2,789,900 cella/ l at surface layer and from 52 to 165,714 cells/ l at bottom layer, respectively. The distribution of standing crops by size class of dinoflagellate species showed that a group of 20-um size class was predominant throughout the present survey, since the most of dominant species have belonged to the size category. Among dominant species, Gyrodinium fissum was most dominant throughout the year in all sampling stations, particulary in June. Scripsiella trochoidea was predominant in May, Protoperidinium bipes and Prorocentrum minimum from June to September, Prorocentrum triestinum, P micans and Ceratium fusus from September to December, and Heterocapsa triquetra from December to April. The stepwise multiple regression analysis between dinoflagellate and environmental parameters revealed that salinity, nitrate-N, pH, and transparency were correlated with the variations of standing crops of dinoflagellate.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.4 no.4
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• pp.289-297
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• 1999

Concentrations of total dissolved free amino acids (DFAAs) in the northeast Pacific Ocean $9^{\circ}54'-10^{\circ}27'N$, $131^{\circ}43'-131^{\circ}53'W$) ranged from 15.9 to 1778.2 17M, and the average was 407.2 nM. Concentrations of DFAAs in surface mixed layers, ranged from 60.1 to 1411.9 nM, and the average was 535.2 nM. Seasonal thermoclines with maxima were formed between the depth of 50 to 150 m. DFAAs in this layer were varied in concentrations from 91.7 to 1778.2 nM, and the average was 588.5 nM. DFAAs below the seasonal thermoclines fluctuated from 15.9 to 384.2 nM, and the average was 175.1 nM. Consequently, in average relatively abundant DFAAs were observed in the subsurface layer than the deeper layer. DFAA vertical profiles and compositions of station A showed similar to station Band C. Glycine, alanine, glutamic acid, serine and valine were predominant accounting for more than 60% of total amino acids. Isoleucine, tyrosine, methionine and phenylalanine comprised only few percents of total DFAAs in the study area. In mole % of amino acid, according to characteristics of functional group of amino acid, aliphatic neutral accounted for 59% and aliphatic hydroxy 16%, acidic 12%, respectively. Although differences in DFAA concentrations with depth were observed, the amino acid composition and mole % of deeper layers in all stations were similar to those of subsurface layers. The results indicate that individual DtAAs remains invariably in water columns relative to the compositions and distributions of DFAAs in the study area, which may be the result of close coupling between microbial activity and their water solubility.