• BMB Reports
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• 제50권10호
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• pp.522-527
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• 2017

A large number of transcriptional activation domains (TADs) are intrinsically unstructured, meaning they are devoid of a three-dimensional structure. The fact that these TADs are transcriptionally active without forming a 3-D structure raises the question of what features in these domains enable them to function. One of two TADs in human glucocorticoid receptor (hGR) is located at its N-terminus and is responsible for ~70% of the transcriptional activity of hGR. This 58-residue intrinsically-disordered TAD, named tau1c in an earlier study, was shown to form three helices under trifluoroethanol, which might be important for its activity. We carried out heteronuclear multi-dimensional NMR experiments on hGR tau1c in a more physiological aqueous buffer solution and found that it forms three helices that are ~30% pre-populated. Since pre-populated helices in several TADs were shown to be key elements for transcriptional activity, the three pre-formed helices in hGR tau1c delineated in this study should be critical determinants of the transcriptional activity of hGR. The presence of pre-structured helices in hGR tau1c strongly suggests that the existence of pre-structured motifs in target-unbound TADs is a very broad phenomenon.

• Macromolecular Research
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• 제17권2호
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• pp.104-109
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• 2009

Solid-state facilitated, olefin transport membranes were prepared by complexation of poly(styrene-b-iso-prene-b-styrene) (SIS) block copolymer and silver salt. Facilitated olefin transport was not observed up to a silver mole fraction of 0.14, representing a threshold concentration, above which transport increased almost linearly with increasing silver salt concentration. This was because firstly the silver ions were selectively coordinated with the C=C bonds of PI blocks up to a silver mole fraction of 0.20, and secondly the coordinative interaction of the silver ions with the aliphatic C=C bond was stronger than that with the aromatic C=C bond, as confirmed by FT-Raman spectroscopy. Small angle X-ray scattering (SAXS) analysis showed that the cylindrical morphology of the neat SIS block copolymer was changed to a disordered structure at low silver concentrations ($0.01{\sim}0.02$). However, at intermediate silver concentrations ($0.15{\sim}0.20$), disordered-ordered structural changes occurred and finally returned to a disordered structure again at higher silver concentrations (>0.33). These results demonstrated that the facilitated olefin transport of SIS/silver salt complex membrancs was significantly affected by their coordinative interactions and nano-structural morphology.

• Macromolecular Research
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• 제14권2호
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• pp.132-139
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• 2006

The dispersion of organoclay in ethylene propylene diene terpolymer (EPDM) matrix was correlated with the rheological and swelling properties of nanocomposites. X-ray diffraction pattern (XRD) and transmission electron microscopic (TEM) analysis exhibited the disordered-intercalated structure of EPDM/organoclay nanocomposite. The extent of the disordered phase increased with increasing organoclay content up to a limiting value of 3 wt% after which equilibrium tended towards intercalation. The dispersion effect of organoclay in EPDM matrix was clarified by the physicochemical properties like rheological response and swelling thermodynamics in toluene. The increase in viscoelastic properties of EPDM nanocomposite with increasing organoclay content up to 3 wt%, followed by a subsequent decrease up to 4 wt%, was correlated in terms of the disordered and ordered states of the dispersed nano-clay sheets. Swelling measurements revealed that the change in entropy of the swelling increased with the increase in disorder level but decreased with the increase in intercalation level of organoclay in the disordered-intercalated nanocomposite. The increase in solvent uptake was comparable with the free volume in EPDM matrix upon inclusion of silicate particles, whereas the inhibition in solvent uptake for higher organoclay loading was described by bridging flocculation.

• 대한기계학회논문집A
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• 제20권3호
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• pp.910-932
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• 1996

A problem of determining the effective conductivity of a useful model of sphere-matrix type, disordered three-phase composite media is considered. Specifically, a three-phase media in which two-phase composite spheres, consisting of spheres of conductivity $k_2$((phase 2) and concentric shells of conductivity $k_3$(phase 3), are randomly distributed in a matrix of conductivity $k_1$( (phase 1) is considered. As for the structure models configuring three-phase composite media, three different structure models of PCS, PS-1 and PS-2 models are defined, which are analogous to well-established PCS, PS structure models of two-phase composite media. Futhermore, a generalized PS-PCS structure model is proposed to incorporate thesee three different models in one. Effective condectivity $k^{\ast}$of multiphaes composite media is greatly influenced by the phase connectivity of each disspersed phase material, as well as phase conductivities and phase volume fractions. Phase connectivity of three-phase PCS, PS-1, PS-2 composite media is quantified by the impentrability parameter $\lambda$. Mathematically rigorous first-order cluster bounds on $k^{\ast}$ are derived for these models of three-phase composite media, and as computation examples, first-order cluster bounds on $k^{\ast}$ for three-phase composites consisting of largely different phase conductivities are computed and compared as function of concnectivity parpmeter $\lambda$. Results and discussions are given.

• 한국결정학회지
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• 제12권2호
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• pp.81-87
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• 2001

We have studied the effect of sintering temperature and time on the cation ordering and second phase formation in Ba(Mg/sub 1/3/Nb/sub 2/3/)O₃(BMN) microwave ceramics by using transmission electron microscopy. The relationship between the structural-chemical behavior arid microwave dielectric properties has also been investigated. It is revealed that according to the sintering conditions the BMN ceramics show very diverse local ordering behavior, such as the development of domain twinning and "core-shell"-structured grains and the formation of local disordered domains, though having 1 : 2 cation ordering structure basically. The disordered structure is found in Mg-excess region. Such local chemical variation seems to be caused by the formation of BaNb₂O/sub 6/-like second phase in its neigh-boring grain boundary. The microwave dielectric quality factor of the ceramics decreases greatly with the increase of the structural-chemical inhomogeneity and diversity.

• 생명과학회지
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• 제9권6호
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• pp.646-652
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• 1999

The effect of temperature on the structure change of the SH of myosin head have been investigated with improved resolution by x-ray diffraction using synchrotron radiation. The movement of myosin head and conformational change of contractile molecules were occurred in the muscle contraction. IASL (iodo acetamide) and MSL (maleimide) disordered the orderly helix arrangement of myosin in the rest state of spin level. The temperature effect on the structure change was great at the UL in the equatorial reflection. But those of IASL and MSL were minor. Equatorial reflection (10, 11) change inferred that myosin head was moved to the vicinity of actin filament by temperature change (from $25^{\circ}C$ to $0^{\circ}C$) at UL, but spin level was not changed. The intensity change of 143 $\AA$ and 72 $\AA$ could offer information of the mass profection of population of myosin heads along the filament axis. The slope of intensity profile of the mass profection of 143$\AA$ and reflection of MSL is appeared sharply and those of UL and IASL were not changed. The decrease of MSL actin reflection at 51 $\AA$ and 59 $\AA$ in the actin reflection change refers that the shifted myosin head binds a certain actin or changes an actin structure. From these results, we could conclude that IASL and MSL were spin labeled on SH of myosin head and disordered the helix arrangement of actin.

• Journal of Pharmaceutical Investigation
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• 제26권1호
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• pp.55-59
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• 1996

The crystal structure of diflunisal, 2',4'-difluoro-4-hydroxy-3-biphenyl-carboxylic acid, was determined by single crystal X-ray diffraction technique. The compound was recrystallized from a mixture of acetone and water in monoclinic, space group C2/c, with $a\;=\;34.666(6),\;b\;=\;3.743(1),\;c\;=\;20.737(4)\;{\AA},\;{\beta}=\;110.57(2)^{\circ}$, and Z = 8. The calculated density is $1.324\;g/cm^3$. The structure was solved by the direct method and refined by full matrix least-squares procedure to the final R value of 0.045 for 1299 observed reflections. It was found that the molecules in the crystal are partially disordered, that is, the two equivalent conformers $(180^{\circ}$ rotated ones through C(1)-C(7)) are packed alternatively without regular symmetry or sequence. The two phenyl rings of the biphenyl group is tilted to each other by the dihedral angle of $43.3^{\circ}$. The carboxyl group at the salicylic moiety is just coplanar to the phenyl ring, and the planarity of this salicylic moiety is stabilized by an intramolecular hydrogen bond of O(3)-H(O3) O(2). The molecules are dimerized through the intermolecular hydrogen bonds at the carboxyl group in the crystal.

• 대한전기학회논문지:전기물성ㆍ응용부문C
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• 제49권6호
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• pp.328-334
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• 2000

A lithium ion secondary battery using carbon as a negative electrode has been developed. Further improvements to increase the cell capacity are expected by modifying the structure of the carbonaceous material. There are hopes for the development of large capacity lithium ion secondary batteries with long cycle, high energy density, high power density, and high energy efficiency. In the present paper, needle cokes from petroleum were examined as an anode of lithium ion secondary battery. Petroleum cokes, MCL(Molten Caustic Leaching) treated in Korea Institute Energy Research, were carbonized at various temperatures of 0, 500, 700, $19700^{\circ}C$ at heating rate of $2^{\circ}C$/min for lh. The electrolyte was used lM liPF6 EC/DEC (1:1). The voltage range of charge & discharge was 0.0V(0.05V) ~ 2.0V. The treated petroleum coke at $700^{\circ}C$ had an initial capacity over 560mAh.g which beyond the theoretical maximum capacity, 372mAh/g for LiC6. This phenomena suggests that carbon materials with disordered structure had higher cell capacity than that the graphitic carbon materials.

• Bulletin of the Korean Chemical Society
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• 제29권6호
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• pp.1229-1232
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• 2008

The surface structure, electrochemical behavior, and wetting property of self-assembled monolayers (SAMs) formed by dioctyl diselenide (DODSe) on Au(111) were investigated by scanning tunneling microscopy (STM), cyclic voltammetry (CV), and contact angle measurements. In contrast to the formation of well-ordered SAMs by octanethiol on Au(111), the SAMs formed by DODSe have a disordered phase and many unusual vacancy islands (VIs). In addition, the formation of DODSe SAMs is largely influenced by the solution concentration used. DODSe SAMs formed in 5 $\mu$ M and 50 $\mu$ M solutions have two mixed domains consisting of missing-row ordered phases and disordered phases, while DODSe SAMs formed in 1 mM and 5 mM solutions have only disordered phases with an abnormally high VI fraction of 22-24%. We also found that the wetting property and electrochemical behavior of DODSe SAMs on Au(111) are markedly influenced by the formation of ordered SAMs and the density of VIs.

• 한국진공학회지
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• 제6권S1호
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• pp.53-58
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• 1997

It is well known that the equiatomic FeAl alloy crystallizes in a paramagnetic CsCl structure and is very stable in a wide temperature range owing to a significant charge transfer from Al to Fe. A presence of structural defects normally enhances the magnetic and magneto-optical properties of this alloy. In this study spin-resolved photoemission and magnetic circular dichroism (MCD) were carried out on both ordered and disordered $Fe_{0.52}Al_{0.48}$ alloy films. The disordered state in the alloy films was obtained by a vapor quenching deposition on cooled substrates. It is shown that the order-disorder transition in the Fe0.52Al0.48 alloy films leads to a significant change in the spin polarization. Form the MCD results the orbital and spin magnetic moments of the constituent atoms are obtained. According to the sum rule the spin and orbital magnetic moments of Fe in the disordered FeAl film are $\mu\frac{SR}{spin}=0.8\mu_B$ and $\mu\frac{SR}{orb}=0.14\mu_B$ respectively. The spin magnetic moment is also evaluated to be $\mu\frac{BR}{spin}=0.77\mu_B$ by the branching ration method employing a photon polarization of 90%.