• 동아시아식생활학회지
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• 제16권2호
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• pp.199-206
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• 2006

This study was designed to investigate the effect of medicinal herb extract on the antioxidant and antimicrobial activities against Helicobacter pylori, which is known as a ulcerogenic pathogen. The concentration of total phenolic compound of Scutellaria baicalensis(13.14%) was highest among water extracts and that of Ulmus parvifolia(15.12%) was highest among the ethanol extracts. The antioxidant activity of the water extract of Scutellaria baicalensis and of the ethanol extract of Ulmus parvifolia were 91.00% and 65.03%, respectively, in DPPH assay. The antioxidant activity of the water extract of Scutellaria baicalensis and of the ethanol extract of Ulmus parvifolia were 32.90% and 27.70%, respectively, in SOD assay. The antioxidant activity of the water extract of Ulmus parvifolia and of the ethanol extract of Glycyrrhiza uralensis Fischer was 2.15 and 2.17, respectively, in TBARS assay. In disc method, Scutellaria baicalensis showed the highest anti-microbial activity against H. pylori, followed by Glycyrrhiza uralensis Fischer among the water extracts and Glycyrrhiza uralensis Fischer showed the highest anti-microbial activity followed by Radix puerariae among ethanol extract.

• 한국환경성돌연변이발암원학회지
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• 제18권1호
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• pp.32-36
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• 1998

It is previously reported that 6-paradol can induce prolonged analgesia in experimental animals. In order to investigate the mutagenicity of 6-paradol, Ames Samonella/microsome plate assay was carried out with Salmonella typhimurium strains, TA 98, TA 100, TA 1535 and TA 1538, 6-Paradol was nonmutagenic in Salmonella typhimurium with and without rat liver microsomal activation. The rec assay with Bacillus substilis strains H 17 $rec^+$ and M 45 $rec^+$ was carried out ot test 6-paradol and other compounds (1-3 mg/disc) for DNA damaging activity, 6-Paradol was also nonmutagenic in DNA damaging activity. The relative size of the inhibition zone for 6-paradol was smaller than that of capsaicin. We have also determined the pathological effects of this compound on the various tissues of rats after administrating(i.p.) with increasing doses of 4, 8, 12, 16 mg/kg at 2 hour intervals and found no significant changes in terms of histology.

• 한방재활의학과학회지
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• 제23권3호
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• pp.15-26
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• 2013

Objectives The aim of this study was to investigate antimicrobial activity of Huanggeumjakyak- tang water extract against MRSA. Methods The antibacterial activities of Huanggeumjakyak-tang were evaluated against 3 strains of Methicillin-resistant Staphylococcus aureus (MRSA) and 1 standard Methicillinsusceptible S. aureus (MSSA) strain by using the disc diffusion method, minimal inhibitory concentrations (MICs) assay, colorimetric assay using MTT test, checkerboard dilution test and time-kill assay was performed under dark. Results The MIC (minimum inhibitory concentration) of Huanggeumjakyak-tang water extract against S. aureus strains ranged from 1,000 to $2,000{\mu}g/ml$. So we confirmed that it has a strong antibacterial effect. Also the combinations of Huanggeumjakyak-tang water extract and conventional antibiotics exhibited improved inhibition of MRSA with synergy effect. Conclusions The results obtained in this study suggest that Huanggeumjakyak-tang water extract showed antibacterial effect against MRSA, and it also showed reducing effect on the side-effect problems that are the major weak points of traditional antibiotics.

• 생약학회지
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• 제50권2호
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• pp.112-117
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• 2019

In this study, extracts of bark from Ulmus davidiana var. japonica and its fractions were investigated the antioxidative, antibacterial and tyrosinase inhibition activity for physiological activity towards functional applications. In the measurement of DPPH radical scavenging activity, the ethyl acetate fraction showed higher radical scavenging ability than others. Moreover, in the tyrosinase inhibition assay, showed that the ethyl acetate fraction has good inhibition effects. Results of the DPPH radical scavenging and tyrosinase inhibition activity are related with the total polyphenol concentrations of ethyl acetate fraction. In antibacterial activity used to find out by utilizing the disc diffusion assay, chloroform fraction showed strong effect against Staphylococcus aureus and S. epidermidis. These results are related with the flavonoid contents of chloroform fraction. On the other hand, in the L929 cell viability measurement by MTT assay, the hexane, butanol and aqueous fraction treated at high concentration were showed cytotoxicity. But the others samples were exhibited a moderate viabilities. As a result of investigated the antioxidant and tyrosinase inhibition activity, the ethyl acetate fraction could be applicable for cosmetics related fields. And the chloroform fraction showed significant antibacterial activity against S. aureus and S. epidermidis.

• 한국환경보건학회지
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• 제44권3호
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• pp.293-300
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• 2018

Objectives: We investigated the antioxidant and antibacterial activities of Pinus densiflora ethanol extracts (PDEE) treated with trypsine as a protease. Methods: Various antioxidant activities were evaluated by measuring total contents of polyphenol and flavonoid, DPPH electron-donating ability and $ABTS^+$ radical scavenging activity of test material. To compare the antibacterial activity, paper disc diffusion assay was performed against two resident bacteria in human skin (Staphylococcus aureus and Staphylococcus epidermidis). Results: As for the total contents of polyphenol and flavonoid, and the electron-donating ability and ABTS+ radical scavenging activity, both PDEE and trypsin-treated Pinus densiflora ethanol extract (T-PDEE) showed high antioxidant activity in dose-dependent manner. And the T-PDEE showed slightly higher activity than PDEE, which indicated protease treatment seemed to affect in antioxidant activity. In the result of paper disc diffusion assay, antibacterial activity was confirmed in all two types of skin resident bacteria. T-PDEE was more active than PDEE and it seems that treatment of protease may increase the antibacterial activity of PDEE. Conclusion: All of these results, we confirmed that treatment of protease to PDEE can increase the antioxidant and antibacterial activities, and it can be explained thought that this would be applicable as a cosmeceutical material in the future.

• Journal of Animal Science and Technology
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• 제44권4호
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• pp.491-498
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• 2002

본 실험에서는 lactobacilli와 bifidobacteria에 의한 E. coli와 S. typhimurium에 대한 생육 억제능을 조사 하였다. 생육억제활성은 well disc assay와 turbidimetry method로 측정하여 비교하였다. 시험균주 10종 중에서 두 가지 방법 모두 평균적으로 높은 항균 활성을 나타낸 균주는 L. acidophilus La-5, L. acidophilus NCFM와 L. casei Lc-01인 것으로 나타났다. 선택된 3가지 유산균과 대표적 enteropathogen인 E. coli와 S. typhimurium을 각각의 혼합 배양하여 생균수를 측정한 결과 병원성균 생육억제는 생균수가 급격히 감소하는 배양 9시간 이후부터 나타났으며 30시간 혼합배양 후에는 병원성균의 생균수는 거의 존재하지 않았다. 실험결과 유산균의 항균활성은 pH 저하 만에 의한 것이 아니었고 유산균의 종류에 따라 생육억제활성에 차이가 있었다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.707-710
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• 2005

항균력이 있는 것으로 예측되는 몇몇 천연물로부터의 추출물과 유산균인 L. mesenteroides, L. citreum, P. pentosaceus의 대사산물인 박테리오신의 항균특성을 알아보기 위하여 최소억제농도 (MIC) 측정법, disc diffusion assay와 challenge법으로 항균력을 조사하였다. L. mesenteroides, L. citreum, P. pentosaceus 유산균에 의해 생산된 박테리오신은 P. aeruginosa, S. aureus, E. coli, B. subtillis 균에 대하여 뛰어난 항균특성을 보인 반면 C. albicans에 대해서는 상대적으로 약한 항균력을 보였다. 반면에 phenonip과 sandal wood 추출물은 C. albicans에 대해서는 강한 항균력을 나타냈으나 다른 대표균들에 대해서는 매우약하거나 거의 항균력을 나타내지 않았다.

• 한국치위생학회지
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• 제17권1호
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• pp.111-122
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• 2017

Objectives: Coriolus versicolor is an edible mushroom with physiological activities that has been used in traditional medicine. The aim of this study was to investigate the antimicrobial activity of extracts obtained from Coriolus versicolor against oral pathogens. Methods: The antimicrobial activities of various extracts of Coriolus versicolor were examined by disc diffusion assay, and minimum inhibitory concentration (MIC) of these extracts were also was determined by broth dilution method. The growth inhibition effect of extracts was measured at 600 nm for 12 hrs against Streptococcus ratti, Streptococcus criceti, Aggregati--bacter actinomycetemcomitans, Actinomyces viscosus, and Actinomyces israelii. Results: Coriolus versicolor extracts showed antimicrobial activities against all nine oral pathogens through disc diffusion assay. The ethanol extract and ethyl acetate extract differed significantly compared with acetone extract against Streptococcus ratti, Streptococcus criceti, Actinomyces viscosus, Actinomyces israelii and Aggregatibacter actinomycetemcomitans (p<0.05). These extracts exhibited MIC ranges of 2.63 to >10.50 mg/ml against the tested bacteria. The ethanol extract from Coriolus versicolor showed lower MIC values of 2.63 to 5.25 mg/ml. According to the obtained growth curve, the extracts of Coriolus versicolor were more effective against Actinomyces viscosus. Conclusions: The acetone, ethanol, and ethyl acetate extracts from Coriolus versicolor showed antimicrobial activities against Streptococcus mutans, Staphylococcus aureus, Streptococcus sanguinis, Streptococcus sobrinus, Streptococcus ratti, Streptococcus criceti, Aggregatibacter actinomycetemcomitans, Actinomyces viscosus, and Actinomyces israeli.i Therefore, they could be considered as natural oral antimicrobial agents against oral pathogens.

• 한방안이비인후피부과학회지
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• 제30권2호
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• pp.100-111
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• 2017

Objectives : The purpose of this study was to research the antibiotic, antioxidant and whitening effects of Jwa Kum-Whan(JKW) and Soo Ryeon-Whan(SRW). Both Jwa Kum-Whan and Soo Ryeon-Whan are composed of Coptidis rhizoma and Evodiae fructus, but the ratios of the two species are different. JKW is composed of Coptidis rhizoma and Evodiae fructus by ratio of 6:1 and SRW's ratio is 1:1. Methods : Antibiotic activities of JKW and SRW's water extracts were studied by paper disc diffusion method. Four kinds of bacteria were applied in paper disc, and each extract was dropped, individually. The diameter of inhibition zone was measured. DPPH assay was used for studying free radical scavenging activity. DPPH is quantitatively decolorized from purple color by antioxidant material. Change of color was measured by spectrophotometer. Whitening activity was analyzed by tyrosinase inhibition assay. Tyrosinase is major enzyme for control process of making eumelanin. Optical Density was measured by spectrophotometer. Results : Candida albicans and Staphylococcus aureus's inhibition zone were made large available by Both prescriptions. Inhibition zone's diameter of JKW was preferable to SRW's. Radical scavenging activity was better at SRW, but JKW's activity was available, too. Tyrosinase inhibition activity was found out available only for JKW, not for SRW. Conclusions : JKW had antibiotic effects for Candida albicans, Staphylococcus aureus. And had antioxidant, whitening effects. SRW had antibiotic, antioxidant activities but not whitening effect.

• 생명과학회지
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• 제15권6호
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• pp.948-954
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• 2005

민간에서 만성간염, 수종, 옹종, 습진, 류마치스성 관절통, 소변분리, 말라리아, 황달을 치료하는데 사용하며, 해열 진통제로 약용하고 있는 배풍등을 metanol (SLM)로 먼저 추출하고 이를 hexane (SLMH), ethylether (SLMEE), ethylacetate(SLMEh), butanol (SLMB) 및 수층 (SLMA) 등 다섯가지의 각 용매별로 분획하여 배풍등의 항균 및 항발암 효과를 연구하였다. 먼저 paper disc method를 이용하여 배풍등의 항균효과를 알아보았다. P. mirabilis, S. aureus, S. marcescens 및 B. substilis의 4가지 사용균주에 배풍등의 각 분획물을 처리한 결과, 모든 균주에서 SLMEA층에서 가장 높은 항균 활성 효과를 나타내었고, 그 다음으로는 SLMEE에서 항균 활성 효과를 나타내었다. 배풍등의 암세포 증식억제 효과(cytotoxicity)를 MTT assay로 실험한 결과, 4종의 암세포주 HeLa, MCF-7, HT-29 및 HepG2 모두 배풍등의 ethylether 분획층인 SLMEE총에서 가장 높은 암세포 증식억제 효과를 보였으며, 암예방 QR유도 활성을 HepG2 세포주를 이용하여 실험한 결과에서도, 다른 분획층에 비해 비극성 용매층인 SLMEE층에서 유의적으로 QR유도 활성을 증가시키는 것으로 나타났다. 이상의 실험 결과를 미루어 볼 때, 극성과 비극성을 둘 다 가지는 SLMEA층에서의 항균활성 물질과 비극성 용매층인 SLMEE 층에서의 암 예방 물질의 단계적인 분리 동정을 통한 생리활성 물질의 개발이 기대되어진다.