• Journal of Microbiology and Biotechnology
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• 제18권2호
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• pp.248-254
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• 2008

Candida magnoliae, an osmotolerant and erythritol producing yeast, prefers D-fructose to D-glucose as carbon sources. For the investigation of the fructophilic characteristics with respect to sugar transportation, a sequential extraction method using various detergents and ultracentrifugation was developed to isolate cellular membrane proteins in C. magnoliae. Immunoblot analysis with the Pma1 antibody and two-dimensional electrophoresis analysis coupled with MS showed that the fraction II was enriched with membrane proteins. Eighteen proteins out of 36 spots were identified as membrane or membrane-associated proteins involved in sugar uptake, stress response, carbon metabolism, and so on. Among them, three proteins were significantly upregulated under the fructose supplying conditions. The hexose transporter was highly homologous to Ght6p in Schizosaccharomyces pombe, which was known as a predominant transporter for the fructose uptake of S. pombe because it exhibited higher affinity to D-fructose than D-glucose. The physicochemical properties of the ATP-binding cassette transporter and inorganic transporter explained their direct or indirect associations with the fructophilic behavior of C. magnoliae. The identification and characterization of membrane proteins involved in sugar uptake might contribute to the elucidation of the selective utilization of fructose to glucose by C. magnoliae at a molecular level.

• 한국통신학회논문지
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• 제41권7호
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• pp.809-815
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• 2016

본 논문은 줄기세포 재생 치료를 위해 사용되는 배지에 직류전압을 인가하여 배지에 반응되는 전기적인 저항 값, 전류 값 그리고 전압 값의 상태에 대해 분석하였다. 배지에 반응되는 전압은 줄기세포 분화 유도 과정에서 전기적인 자극에 연관되며 반응 전압 상태에 따라 분화의 상태에 대해 확인 할 수 있다. 배지의 반응 전압에서 전압변화 레벨이 적으면 줄기세포 자극 조건이 안정적이며 만일 전압 변화 레벨이 심하면 줄기세포 자극 조건이 불안정하여 줄기세포 분화 과정에서 상당한 손실을 따르게 될 수 있다. 본 연구는 줄기세포 재생 치료의 가능성을 위해 전기적인 자극 조건의 최적화 하는데 도움이 될 것으로 기대한다.

• 한국병원경영학회지
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• 제18권1호
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• pp.59-69
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• 2013

Purpose Patients should have a regular medical check-up, the cost direct and indirect medical expenses to check the results, and revisit the hospital. Some of patients often want to use the telephone for a medical consultation, Method The subjects participating in this study were a selection 485 patients who received outpatient service in Asan Medical Center and Gangbuk Samsung Hospital. The patients's favorite method for medical check-up, time, cost, fatigue, and inconvenience, in regard with the revisit to check the examination results, were researched and analyzed on identical questionnaires about them. Results The average transportation, food expenses and opportunity costs the subjects spent was 90,400 for those from the Seoul and Gyunggi area, and 269,800 from other area. And the average required time per visit was 4.13 hours for those from the Seoul and Gyunggi area, 11.84 hours from other areas, and averagely 7.72 hours for all the subjects. More than 75% of the subjects answered that they felt tired and their work was interrupted. The subjects from both areas preferred the check by phone. Conclusion If the scope of telephone medical consultation is defined and the doctor-patient certification is possible, it can be thought as an efficient alternative of the medical check-up by revisit. It is necessary to discuss an appropriate amount of the medical check-up by phone which the same cost and effort can be allocated in.

• 융합보안논문지
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• 제6권4호
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• pp.141-151
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• 2006

지난 2003년 1월 25일 인터넷 마비사고와 2004년 주요 국가기관 해킹사건을 겪으면서 사이버 위협의 파괴력이 국가안보에 직접적이고 심각하게 위협하는 단계에까지 도달하였다. 그래서 사이버테러나 사이버전(cyber warfare)은 더 이상 가상적 상황이 아닌 현실적이며, 실체적인 안보상황으로 상대국의 군사지휘체계는 물론 통신, 에너지, 금융, 수송체계 등 국가 주요기능 무력화의 전쟁 개념의 확대로 재인식되고 있다. 이러한 시점에서 정보전, 정보작전, 네트워크 중심전등 혼재된 유사 용어들 속에서 사이버전에 대한 명확한 개념 정립 필요하고 각 국의 사이버전 동향 분석 및 국내 사이버전 현황 분석을 통해 문제점을 식별, 보완책 마련이 필요하다. 그래서 본 논문에서는 국가적 위기를 효율적으로 관리하고, 효과적이며 능동적으로 사이버전을 수행할 수 있는 발전방향과 상대적으로 사이버전 관련 기술 및 전문 인력 운영적측면에서 열세에 놓여 있는 군의 사이버전 수행체계에 대한 혁신방향을 연구를 하였다.

• Journal of Preventive Medicine and Public Health
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• 제43권4호
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• pp.352-361
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• 2010

Objectives: This study estimated the annual socioeconomic costs of food-borne disease in 2008 from a societal perspective and using a cost-of-illness method. Methods: Our model employed a comprehensive set of diagnostic disease codes to define food-borne diseases with using the Korea National Health Insurance (KNHI) reimbursement data. This study classified the food borne illness as three types of symptoms according to the severity of the illness: mild, moderate, severe. In addition to the traditional method of assessing the cost-of-illness, the study included measures to account for the lost quality of life. We estimated the cost of the lost quality of life using quality-adjusted life years and a visual analog scale. The direct cost included medical and medication costs, and the non-medical costs included transportation costs, caregiver's cost and administration costs. The lost productivity costs included lost workdays due to illness and lost earnings due to premature death. Results: The study found the estimated annual socioeconomic costs of food-borne disease in 2008 were 954.9 billion won (735.3 billion won-996.9 billion won). The medical cost was 73.4 -76.8% of the cost, the lost productivity cost was 22.6% and the cost of the lost quality of life was 26.0%. Conclusions: Most of the cost-of-illness studies are known to have underestimated the actual socioeconomic costs of the subjects, and these studies excluded many important social costs, such as the value of pain, suffering and functional disability. The study addressed the uncertainty related to estimating the socioeconomic costs of food-borne disease as well as the updated cost estimates. Our estimates could contribute to develop and evaluate policies for food-borne disease.

• 대한기계학회논문집B
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• 제34권7호
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• pp.689-696
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• 2010

디젤엔진은 높은 열효율과 우수한 연비 성능 등의 장점을 가지고 있으나, 기화된 연료와 주변 공기가 혼합된 후 착화되는 과정을 거치기 때문에 이론 공연비 영역에서는 질소산화물(NOx) 배출이 증가되는 문제점을 가지고 있다. 최근 활발히 연구되고 있는 높은 정화 효율을 가진 LNT 촉매는 희박 분위기 조건에서는 NOx를 흡장하게 되고 과농한 분위기에서는 환원 분위기 형성을 통하여 NOx를 저감시키게 된다. 희박 공연비에서 동작하는 디젤 엔진에서는 이러한 환원 과정을 이루기 위해 주기적으로 과농한 분위기를 형성해주어야만 하는데, 이러한 연구는 NOx 저감을 위해 HC를 포함한 환원제의 농도를 제어하는 기술로서 본 연구에서는 디젤을 연료로 하는 수소농후가스 발생장치를 이용하여 LNT 촉매에 환원제로서 수소농후가스를 직접 공급하는 방식으로 LNT 촉매의 NOx 저감 특성을 파악하였다.

• 한국응급구조학회지
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• 제5권1호
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• pp.7-14
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• 2001

The purposes of this study which was conducted by inquiring into some curricula for Emergency Medical Dispatchers(EMDs) are to introduce a curriculum into Korea and develop a textbook for training of the EMDs in Korea. The conclusions from this study were summarized as follows; (1) There are some professional demands in our Emergency Medical Dispatch field therefore, now it is necessary to set up an education system for the EMDs in Korea and give them a suitable name to this change. (2) Some historical turning points which are condensed from the developmental process in the USA. including "Medical Self Help" with no formal dispatch protocols, Medical Priority Dispatch System, Practice Standard, Dispatch Protocols will serve as a good reference for establishing and education system for the EMDs. (3) To effectively train the EMDs in Korea, we needed to be introduced to Emergency Medical Dispatch : National Standard of Curriculum from the U.S. Department of Transportation & the National Highway Traffic Safety Administration which is made up Basic Emergency Medical Concepts, Information Gatherin & Dispatch, Introduction to the Emergency Medical Dispatch Protocol Reference System(EMDPRS) & 32 Chief Complaint Types. (4) The introduced curriculum closely related to the medical director, the direct indirect medical control, the scope of practice in the USA, should be revised for the Emergency Medical Service System of Korea. But to reduce the developmental process & time, it is necessary to postpone the consideration on these factors until publishing a new textbook. (5) This study which gives the cornerstone about some developmental methods on textbook for EMDs will be helpful to build up an education system for EMDs such as Curriculum, EMD Certification, National Academy of Emergency Medical Dispatch to fit the Emergency Medical Service System of Korea in the future.

• Structural Engineering and Mechanics
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• 제72권6호
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• pp.675-687
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• 2019

As the bridges are an integral part of the transportation network, their function as one of the most important vital arteries during an earthquake is fundamental. In a design point of view, the bridges piers, and in particular the wall piers, are considered as effective structural elements in the seismic response of bridge structures due to their cantilever performance. Owing to reduced seismic load during design procedure, the response of these structural components should be ductile. This ductile behavior has a direct and decisive correlation to the development of plastic hinge region at the base of the wall pier. Several international seismic design codes and guidelines have suggested special detailing to assure ductile response in this region. In this paper, the parameters which affect the length of plastic hinge region in the reinforced concrete bridge with wall piers were examined and the sensitivity of these parameters was evaluated on the length of the plastic hinge region. Sensitivity analysis was accomplished by independently variable parameters with one standard deviation away from their means. For this aim, the Monte Carlo simulation, tornado diagram analysis, and first order second moment method were used to determine the uncertainties associated with analysis parameters. The results showed that, among the considered design variables, the aspect ratio of the pier wall (length to width ratio) and axial load level were the most important design parameters in the plastic hinge region, while the yield strength of transverse reinforcements had the least effect on determining the length of this region.

• 한국산학기술학회논문지
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• 제11권1호
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• pp.67-72
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• 2010

본 논문에서는 기존의 궤도회로(Track Circuit)를 이용하는 FBS의 설비측면에서 단락감도 및 선로변 설비(지상설비)의 규모에 따른 유지보수의 증가 등의 문제점을 극복하고, 차량간 간격을 근접시키기 위한 열차제어 개념인 MBS의 구현에 따른 무인운전기술의 가능성을 검증하였다. MBS에 의한 열차 운전은 중앙국에서 열차의 위치를 실시간으로 알 수 있어 선행열차의 정보를 후속열차에 직접 전달할 수 있으므로 시격(Headway)단축이 가능해지고 그에 따라 수송수요 증대와 불필요한 가.감속을 방지할 수 있다. 이와 더불어 지상설비를 대폭적으로 감소시킬 수 있으며, 승객서비스 향상 및 승차감 향상 등의 이점을 제공한다.

• 한국응용과학기술학회지
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• 제29권4호
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• pp.561-569
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• 2012

본 연구는 강원도 소재 동해항만에서 발생하는 미세먼지 관리를 위한 환경비용편익을 산출하는 것이다. 항구 인근에 부유하는 미세먼지의 농도는 매우 높은 편이며, 지점에 따라 국가 기준인 $100{\mu}g/m^3$ 이상으로 관측되는 곳도 있다. 시험대상 항구는 주로 석회석과 석탄을 취급함으로써 미세입자상 물질이 하역시 다량 발생한다. 연구결과 PM10을 기준으로 년간 12톤의 미세먼지가 하역작업 시 발생하는 것으로 밝혀졌다. 덧붙여서 원료물질을 비롯한 다양한 화물을 운송하는 대형차량 및 중장비는 디젤 검댕이를 발생하고, 도로먼지의 비산을 유발한다. 지방정부는 해마다 20억원 이상의 비용을 투자하여 대기중 미세먼지를 제거하고 있다. 편익대비 비용을 산출한 결과 그 효과는 최소 240%에서 최대 720%까지 얻을 수 있는 것으로 나타났다.