• Korean Journal of Plant Tissue Culture
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• v.21 no.6
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• pp.319-326
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• 1994

For the gene tagging of Armoracia rusticana, maize controlling element Ac and Ds were introduced into A.rusticana via Agrobacterium-mediated transformation method. We established an efficient in via regeneration and transformation system for gene transfer in A. rusticana. The optimum in via regeneration condition has been obtained from leaf, petiole and root organs on modified MS medium supplemented with NAA 0.1 mg/L plus BA 1.0 mg/L for direct shooting and with free growth regulators for root induction for transformation, the leaf, petiole and root explants of A. rusticana were concultivated with Agrobacterium tumefaciens, LBA4404 which carries a binary vector pEND4K containing maize controlling element Ac or Ds, respectively: Selections were performed in the shoot induction medium supplemented with 100 mg/L kanamycin, and 500 mg/L carbenicillin transformation frequency showed about 8 to 10% in case of leaf disks. PCR md Southern blot analyses showed that the Ac and the Ds elements were integrated into the chromosome of donor plants.

• Journal of the Korean Chemical Society
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• v.35 no.6
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• pp.673-679
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• 1991

The electrochemical reduction of N-tert-butylbenzothiazole-2-sulfenamide (TBBS; vulcanization accelerator) was investigated by direct current, differential pulse polarography, cyclic voltammetry and controlled potential coulometry. The electrode reduction of TBBS proceeded E-C-E-C reaction mechanism by four electrons transfer at irreversible one wave (-2.31 volts vs. Ag/0.1M AgN$O_3$ in AN). As the results of controlled potential electrolysis, mercaptobenzothiazole (MBT), benzothiazole disulfide (MBT dimer) and extricated sulfur were products which followed by cleavage of the sulfenamide (-S-N=) bond. Upon the basis of products analysis and polarogram interpretation with pH variable, electrochemical reaction mechanism was suggested.

• Journal of the Korean Chemical Society
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• v.35 no.6
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• pp.680-688
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• 1991

The electrochemical reduction of N-oxyldiethylenebenzothiazole-2-sulfenamide (ODBS; vulcanization accelerator) was investigated by direct current polarography, differential pulse polarography, cyclic voltammetry and controlled potential coulometry. The irreversible electrode reduction of ODBS proceeded E-C-E-C reaction mechanism by three electrons transfer with irreversible one wave (-1.86 volts vs. Ag/0.1 M AgN$O_3$ in AN). As the results of controlled potential electrolysis, mercaptobenzothiazole (MBT), benzothiazole disulfide (MBT dimer) and extricated sulfur were products which followed by cleavage of the sulfenamide (-S-N=) bond. Upo the basis of products analysis and polarogram interpretation witli pH variable, electrochemical reaction mechanism was suggested.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.12 no.5
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• pp.1-9
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• 2012

In this paper, we presents the modeling and implementation of the DDS(Direct Digital synthesizer) driven offset PLL(Pghase Locked Loop) with DAC(Digital Analog Converter) for coarse tune. The PLL synthesizer was designed for minimizing the size and offset frequency and DDS technique was used for ultra low noise and fast lock up time, also DAC was used for coarse tune. The output phase noise was analyzed by superposition theory with the phase noise transfer function and noise source modeling. the phase noise prediction was evaluated by comparing with the measured data. The designed synthesizer has ultra fast lock time within 6 usec and ultra low phase noise performance of -120 dBc/Hz at 10KHz offset frequency.

• Bulletin of the Korean Chemical Society
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• v.35 no.12
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• pp.3553-3558
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• 2014

The reduced graphene oxide(rGO)/aluminum phosphate($AlPO_4$)-coated $LiMn_{1.5}Ni_{0.5}O_4$ (LMNO) cathode material has been developed by hydroxide precursor method for LMNO and by a facile solution based process for the coating with GO/$AlPO_4$ on the surface of LMNO, followed by annealing process. The amount of $AlPO_4$ has been varied from 0.5 wt % to 1.0 wt %, while the amount of rGO is maintained at 1.0 wt %. The samples have been characterized by X-ray diffraction, scanning electron microscopy, and high-resolution transmission electron microscopy. The rGO/$AlPO_4$-coated LMNO electrodes exhibit better cyclic performance compared to that of pristine LMNO electrode. Specifically, rGO(1%)/$AlPO_4$(0.5%)- and rGO(1%)/$AlPO_4$(1%)-coated electrodes deliver a discharge capacity of, respectively, $123mAhg^{-1}$ and $122mAhg^{-1}$ at C/6 rate, with a capacity retention of, respectively, 96% and 98% at 100 cycles. Furthermore, the surface-modified LMNO electrodes demonstrate higher-rate capability. The rGO(1%)/$AlPO_4$(0.5%)-coated LMNO electrode shows the highest rate performance demonstrating a capacity retention of 91% at 10 C rate. The enhanced electrochemical performance can be attributed to (1) the suppression of the direct contact of electrode surface with the electrolyte, resulting in side reactions with the electrolyte due to the high cut-off voltage, and (2) smaller surface resistance and charge transfer resistance, which is confirmed by total polarization resistance and electrochemical impedance spectroscopy.

• Journal of the Korean Society for Precision Engineering
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• v.28 no.3
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• pp.285-291
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• 2011

The error motion of a machine tool spindle directly affects the surface errors of machined parts. The error motions of the spindle are not desired errors in the three linear direction motions and two rotating motions. Those are usually due to the imperfect of bearings, stiffness of spindle, assembly errors, external force or unbalance of rotors. The error motions of the spindle have been needed to be decreased to desired goal of spindle's performance. The level of error motion is needed to be estimated during the design and assembly process of the spindle. In this paper, the estimation method for the five degree of freedom (5 D.O.F) error motions of the spindle is suggested. To estimate the error motions of the spindle, waviness of shaft and bearings, external force model was used as input data. And, the estimation models are considering geometric relationship and force equilibrium of the five degree of the freedom. To calculate error motions of the spindle, not only imperfection of the shaft, bearings, such as rolling element bearing, hydrostatic bearing, and aerostatic bearing, but also driving elements such as worm, pulley, and direct driving motor systems, were considered.

• Journal of The Korean Astronomical Society
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• v.36 no.2
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• pp.55-60
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• 2003

In Lee, Kang & Byun (2001) the discovery of Raman scattered 6545 A feature was reported in symbiotic stars and the planetary nebula M2-9. The broad emission feature around 6545 A is formed as a result of Raman scattering of He II n = 6 $\to$ n = 2 photons by atomic hydrogen. In this paper, we introduce a method to compute the equivalent width of He II $\lambda$ 1025 line and present an optical spectrum of the symbiotic star RR Telescopii as an example for a detailed illustration. In this spectrum, we pay attention to the broad H$\alpha$ wings and the Raman scattered He II 6545 feature. The broad Ha wings are also proposed to be formed through Raman scattering of continuum around Ly$\beta$ by Lee (2000), and therefore we propose that the equivalent width of the He II $\lambda$ 1025 emission line is obtained by a simple comparison of the strengths of the 6545 feature and the broad H$\alpha$ wings. We prepare a template H$\alpha$ wing profile from continuum radiation around Ly$\beta$ with the neutral scattering region that is supposed to be responsible for the formation of Raman scattered He II 6545 feature. Isolation of the 6545 feature that is blended with [N II] $\lambda$ 6548 is made by using the fact that [N II] $\lambda$ 6584 is always 3 times stronger than [N II] $\lambda$ 6548. We also fit the 6545 feature by a Gaussian which has a width 6.4 times that of the He II $\lambda$ 6527 line. A direct comparison of these two features for RR Tel yields the equivalent width $EW_{Hel025} = 2.3{\AA}$ of He II $\lambda$ 1025 line. Even though this far UV emission line is not directly observable due to heavy interstellar extinction, nearby He II lines such as He II $\lambda$ 1085 line may be observed using far UV space instruments, which will verify this calculation and hence the origins of various features occurring in spectra around H$\alpha$.

• Journal of the Korea Concrete Institute
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• v.15 no.2
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• pp.183-196
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• 2003

This paper evaluates the effects of haunches and the characteristic differences of haunch design regulations through design of pier and box structures with/without haunches. The design of the pier and box structures was conducted by using the linear elastic plane stress finite element analysis, the DIN 1045 and ACI 318-99 codes, the suggested experimental design equations, and the strut-tie model approach. To prove the validity of design results obtained by the strut-tie model approach, the ultimate strength of two haunched reinforced concrete beams tested to failure was evaluated by using the approach. According to the comparison and evaluation of the design results, it is concluded that the design results of haunched reinforced concrete structures by using conventional and design codes need to be complemented with those by using the strut-tie model approach that reflected the effects of haunches in design comparatively well through the actions of arch and direct transfer of applied loads.

• Proceedings of the KIEE Conference
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• 2008.07a
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• pp.1295-1296
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• 2008

Hydrogen peroxide ($H_2O_2$) biosensor is one of the most developing sensors because this kind of sensors is highly selective and responds quickly to the specific substrate. Hemoglobin (Hb) has been used as ideal biomolecules to construct hydrogen peroxide biosensors because of their high selectivity to $H_2O_2$. The direct electron transfer of Hb has widely investigated for application in the determination of $H_2O_2$ because of its simplicity, high selectivity and intrinsic sensitivity. An electrochemical detection for hydrogen peroxide was investigated based on immobilization of hemoglobin on DNA/Fe(pyterpy)$^{2+}$ modified gold electrode. The pyterpy monolayers were firstly an electron deposition onto the gold electrode surface of the quartz crystal microbalance (QCM). It is offered a template to attach negatively charged DNA. The fabrication process of the electrode was verified by quartz crystal analyzer (QCA). The experimental parameters such as pH, applied potential and amperometric response were evaluated and optimized. Under the optimized conditions, this sensor shows the linear response within the range between $3.0{\times}10^{-6}$ to $9.0{|times}10^{-4}$ M concentrations of $H_2O_2$. The detection limit was determined to be $9{\times}10^{-7}$ M (based on the S/N=3).

• KSCE Journal of Civil and Environmental Engineering Research
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• v.8 no.3
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• pp.31-41
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• 1988

Recently, the offshore structures are increasingly constructed to explore the natural resources. These offshore structures are to be designed to resist the repetitive wave forces. A probabilistic method for the fatigue analysis of offshore concrete structures is presented in this study. The present spectral fatigue analysis calculates wave forces first and then the transfer functions for unit waves from which stress spectra are determined. The calculated fatigue stresses may then be used to evaluate the fatigue damage of concrete structures. A simplified model for the estimation of fatigue damage of the structures, which employs only the probabilistic moments of the peak stress distribution without direct integration, is also proposed. The present study allows more realistic fatigue analysis of offshore concrete structures.