• Microbiology and Biotechnology Letters
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• 제42권1호
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• pp.11-17
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• 2014

Selenium is an essential trace element for mammals, but it is very toxic. Therefore, the control of selenium concentrations should be precisely and effectively monitored. Selenium is naturally obtained through foods and seleno-L-methionine (LSeMet) is a major form of selenium. It has been reported that L-SeMet is only converted into Se-adenosyl-L-SeMet. However, a recent study suggested that L-SeMet was directly metabolized into methylselenol ($CH_3SeH$) in mouse liver extract by the reaction of cystathionine ${\gamma}$-lyase (CGL). The canonical reaction of CGL was known to catalyze the cleavage of L-cystathionine to L-cysteine, ${\alpha}$-ketobutyrate and $NH_3$. In the present study, we found that L-SeMet could be directly converted to $CH_3SeH$ using purified homogenous human CGL instead of mouse liver cytosol. Authentic $CH_3SeH$ was prepared by reduction of dimethyldiselenide with sodium tetrahydroborate. The gaseous product of the enzymatic reaction with L-SeMet was analyzed by GC/MS spectrometry. The GC/MS data was identical to that of authentic dinitrophenyl selenoether. We also analyzed the kinetic parameters for the formation of $CH_3SeH$ from L-SeMet by human and mouse CGL. These results suggest that human CGL is a critical enzyme which is responsible for L-SeMet metabolism.

• Journal of the Korean Geographical Society
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• 제41권4호
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• pp.418-434
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• 2006

The study was investigated to runoff characteristics of non-point pollutants according to rainfall in Kokseong river watershed. The result of which is as follows : First of all, major reason which affect the formation of water quality of Kokseong River is judged to be caused by non-point pollution source which flows out from farmland and residential area. Flow of rainfall effluent in the downstream in which direct flow components of urban district and combined sewer overflows of farmland was intervened faster than that in the upstream reacted more promptly. Generation of pollutants by non-point source shows increasing trend in general in accordance with the increase in the intensity of rainfall but it was affected by SS, BOD, COD and T-P in the upstream part whereas BOD, COD and T-N were significantly affected by beginning period of rainfall in the downstream. EMC in the downstream increased approximately 3-315 times as compared to upstream, particularly the discharge of SS5 and T-P were extremely increased. While surface flow out of rainfall effluent in the upstream was only 4.7%, the surface flow in the downstream took up as much as 29%, which was major reason for the increase of EMC. From the above contents, we can see that the change in water quality according to the increase and decrease of effluent at the time of rainfall showed very complex pattern depending on the type of land use, and it is judged that the most important thing for the administration of non-point pollution source is to come up with the solution for the reduction of effluent at the beginning.

• Korean Journal of Weed Science
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• 제18권2호
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• pp.106-115
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• 1998

This study was conducted to investigate the effect of oxadiazon, molinate, thiobencarb before seeding on control of red rice and barnyardgrass in water-seeded rice. High application rate plot among oxadiazon treatment plots could observe phytotoxicity symptoms depending on field conditions, but these injury recovered gradually with time. Molinate and thiobencarb application plots at the concentration of 225~400, 210~420g ai/l0a respectively were not observed phytotoxicity. Control of red rice was different according to kinds of herbicides and application rates. Oxadiazon showed higher control performance at the concentration of more than 60g ai/10a. Control effect of molinate and thiobencarb against red rice was enhanced with the increase of application rate, and both herbicides showed satisfactory effect at more than 300g ai/10a. Control of barnyardgrass showed up to 90~100% in all tested herbicides. There was no significant yield reduction by oxadiazon, molinate, and thiobencarb application before seeding in all tested field. In the pot experiment, crop injury, seedling stand, and early growth were more advantageous at time of drainge after one day after seeding than flooding until rooting.

• Korean Journal of Environmental Biology
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• 제35권4호
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• pp.476-491
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• 2017

The water spider (Argyroneta aquatica), a rare member of its species, is one of the few insects known to live under water all over the world. In Korea, the water spider can be found only in the Eundae-ri, Yeoncheon, Gyeonggi province, where they are designated as a natural monument. However, loss of habitat is obvious, as the Eundae-ri wetland undergoes hydrarch succession. Loss of habitat has a direct impact on the water spider population. Thus protective measures for the water spider are needed to preserve the water spider habitat. In this study, a sweeping and visual survey of insect species was conducted in the water spider habitat from May to September 2015. The characteristics of each one of the different insect species and the community features were analyzed in this study. A total of 3,929 insects representing 276 species from 13 orders collected for the analysis. The most frequently collected insects from the Order Hemiptera represented 24.2% of total species and 82.3% of the total number of insects. Community analyses showed dominance, diversity, evenness, and richness indices of 0.23, 2.27, 0.41, and 31.2, respectively. Compared with the previous survey data, these results showed that reduction of the spider's wetland habitat caused the most commonly collected insects to adapt and spread to the surrounding terrestrial environment.

• Journal of Nutrition and Health
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• 제39권2호
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• pp.160-170
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• 2006

This study was conducted to provide foundation data for making health care policy for married women by assessing the dietary intake between highly educated married, employed and unemployed women. It is a direct interview, cross-sectional study with 24-hour recall method for one day. In selecting the subjects for this study, married, unemployed women were selected from a certain area (Daedeok Science Town) in Daejeon where there are high rates of highly educated women, and the married, employed women were selected from the teaching profession in order to avoid confounding due to including a variety of jobs. According to the Korean Standard Classification of Occupations, teaching is the representational occupation of highly educated, married women. Then, to prevent confounding due to age, we selected the subjects out of each age group at the same rate through random sampling. Women who had not graduated college, worked only part-time, or had no current spouse were excluded. As a result, 486 highly-educated, married, unemployed (250) and employed (236) women were used for analyzing data. The unemployed women consumed a higher amount of fat, cholesterol, sodium, vitamin C and folic acid while the employed women consumed a higher amount of iron, vitamin $B_l$ and vitamin $B_2$. P/M/S ratio being 1/1.18/1.05 and 1/1.05/0.87, for the unemployed women and the employed women, respectively, unemployed respondents had a higher saturated fat intake than those of employed. It is in excess of the standard ratio (1/1/1) of the Korean RDA. At the same time, in unemployed respondents the percent of energy intake from fat (24.8%, 23.2%) and animal fat (12.4%, 11.4%) were higher than those of employed respondents. The mean daily nutrient intake of calcium, zinc, and iron for both groups of respondents were lower than the Korean RDA. Both groups had phosphorus as the highest nutrient and calcium as the lowest nutrient of INQ (Index of Nutritional Quality) while nutrients with the INQ being less than 1 were calcium and iron. To sum up, the following conclusions can be made: Nutrition education and guidance for reduction of the intake of fat, especially animal fat, are necessary for unemployed women. In addition, highly educated, married, unemployed and employed women should increase the consumption of foods rich in iron and calcium to prevent anemia and osteoporosis, while decreasing the intake of phosphorus to balance proportions of calcium and phosphorus.

• Journal of the Korean Society of Food Science and Nutrition
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• 제26권4호
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• pp.743-750
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• 1997

Direct measurement of the kinetics of free fatty acid transfer between phospholipid model membrane is technically limited by the rapid nature of the transfer process. Separation of membrane-bound fatty acid by centrifugation has shown that although the equilibrium distribution of free fatty acid is determined by this method, fatty acid transfer occurs too rapidly for accurate kinetic measurements. Recently fluorescence resonance energy transfer(FRET) assay has been developed to examine transfer of fatty acids between membranes. Donor membranes which has fluorescent fatty acid, anthroyloxy fatty acid(AOFA), is mixed with acceptor membranes which has non-interchangeable fluorescent quencher, nitrobenzo-xadiazol(NBD), using stopped flow apparatus. As the fluorescent fatty acids transfer from donor membrane to acceptor membrane, fluorescence intensity would be decreased and the rate and degree of fatty acid transfer can be analyzed. Fatty acid transfer between micelles is more complicated because of bile salt. Therefore in experiments with micelles, fluorescence self quenching assay is used. At high concentrations, a fluorophore tends to quench its own fluorescence causing a reduction in fluorescence intensity. Donor micelles contained self quenching concentrations of fluorophore and acceptor micelles had no fluorophore. Upon mixing of donor and acceptor micelles, the rate of transfer of the fluorophore from the donor to the acceptor was measured by monitoring the release in self quenching when its concentration in donor decreased over time.

• Korean Journal of Remote Sensing
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• 제15권2호
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• pp.91-105
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• 1999

Spatial data integration using multiple geo-based data sets has been regarded as one of the primary GIS application issues. As for this issue, several integration schemes have been developed as the perspectives of mathematical geology or geo-mathematics. However, research-based approaches for statistical/quantitative assessments between integrated layer and input layers are not fully considered yet. Related to this niche point, in this study, spatial data integration using multiple geoscientific data sets by known integration algorithms was primarily performed. For spatial integration by using raster-based GIS functionality, geological, geochemical, geophysical data sets, DEM-driven data sets and remotely sensed imagery data sets from the Ogdong area were utilized for geological thematic mapping related by mineral potential mapping. In addition, statistical/quantitative information extraction with respective to relationships among used data sets and/or between each data set and integrated layer was carried out, with the scope of multiple data fusion and schematic statistical assessment methodology. As for the spatial integration scheme, certainty factor (CF) estimation and principal component analysis (PCA) were applied. However, this study was not aimed at direct comparison of both methodologies; whereas, for the statistical/quantitative assessment between integrated layer and input layers, some statistical methodologies based on contingency table were focused. Especially, for the bias reduction, jackknife technique was also applied in PCA-based spatial integration. Through the statistic analyses with respect to the integration information in this case study, new information for relationships of integrated layer and input layers was extracted. In addition, influence effects of input data sets with respect to integrated layer were assessed. This kind of approach provides a decision-making information in the viewpoint of GIS and is also exploratory data analysis in conjunction with GIS and geoscientific application, especially handing spatial integration or data fusion with complex variable data sets.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• 제29권3호
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• pp.298-309
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• 2019

Objectives: This study aimed to investigate stages of safety and health culture change through a reflection on 40 years of hearing conservation history at a multinational company. Methods: The target workplaces were multinational companies with more than 1,000 employees. The research used the clinical case study and system analysis methods based on direct observation of the research from 1994 to 2009. The latter method performed an analysis of the equilibrium state of the cross-section in the given period and the longitudinal profile of the change during the given period. Results: The stages of cultural change are divided into five stages and summarized as follows. In the first stage, workplace noise was not widely recognized as a hazard, while in the second stage, the measurement of noise levels and audiometric testing were conducted under the Korean Occupational Safety and Health Act (KOSHA). The driving force for change in the second stage was the amendment of the KOSHA. In the third stage, noise came to be recognized as a hazard factor through awareness training. The driving force of change during the third stage was the strong executive power exerted by the audit of the industrial hygiene program from the US head office. In the fourth step, there was a change to actually reduce noise. The driving force in this stage was a change in company executives' risk perception resulting from lawsuits over noise-induced hearing loss and the task force team activities for culture change based on the action learning protocol. At the fifth stage, a 'buy quiet policy' was institutionalized. The management's experience that noise reduction was difficult was the motivation to manage noise from the time of purchase of equipment. Conclusions: The activities of a hearing conservation program are determined by the improvement of the legal system and by the way it is enforced. Noise control activities to reduce noise areas may be possible through the shared risk perception of noise-induced hearing loss and by a change agent role as a facilitator to implement noise control.

• Journal of the Korea Society of Computer and Information
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• 제25권6호
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• pp.155-164
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• 2020

In this paper, we proposed the dimensional system and pattern of middle-aged women's rashguard swimwear for breast cancer patients. First, a survey of 37 breast cancer patients was conducted to determine the preferred swimsuit design for breast cancer patients. According to a survey of breast cancer patients, a rashguard swimwear with a low-exposure chest was designed. It has a pocket shape in which the cap is easy to insert and the cap is fixed. Second, we developed a dimensional system for swimsuits for breast cancer patients. Using direct measurement data from Size Korea for 1,625 women aged 30 to 69, 17-sizes for breast cancer patient's swimsuits were set through cross-analysis of major dimensions, with a coverage rate of 74.3%. It also extracted a standard size of bust circ. 90cm, hip circ. 95cm, and trunk circ. 150cm. Third, we developed a pattern for the central size of the swimsuit for breast cancer patients. For this purpose, we first produced the first central size pattern, and then completed the pattern after modifying it through the second and third wearing experiments. Experimental suits were produced at each pattern-making stage to evaluate wearing fit and motion suitability. Finally, the reduction rate of each dimension item for optimal pattern production was obtained, and the lashguard swimwear pattern for breast cancer patients was developed.

• Journal of Korea Water Resources Association
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• 제52권3호
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• pp.187-194
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• 2019

Intake ratio from river constitutes about 31% (8/26) that beings to "water stress country" as "Medium ~ High" with China, India, Italy, South Africa, etc. Therefore, the present study on a dam that is the most effective and direct for securing water resources has been performed. First of all, climate change scenarios were investigated and analyzed. RCP 4.5 and 8.5 with 12.5 km grid resolution presented in the IPCC (Intergovernmental Panel on Climate Change) 5th Assessment Report (AR5) were applied to study watershed using SWAT (Soil and Water Assessment Tool) and HEC-ResSim models that carried out co-operation. Based on the results of dam simulation, the reduction effects of floods and droughts were quantitatively presented. The procedures of dam projects of the USA, Japan and Korea were investigated. As a result, there are no estimating quantitative criteria, calculating methods or formulas. In the present study, therefore, indexes for selecting suitable dam site through literature investigation and analyzing dam watersheds were determined, Expert questionnaire for various indexes were performed. Based on the above mentioned investigation and expert questionnaire, a methodology assigning weight using AHP method were proposed. The function of suitable dam (FSDS) site was calibrated and verified for four medium-sized watersheds. Finally, automated model for suitable dam site was developed using FSDS and 'Model builder' of GIS tool.