• Title/Summary/Keyword: Direct dye

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Endotoxins of Enteric Pathogens Modulate the Functions of Human Neutrophils and Lymphocytes

  • Islam, Laila N.;Nabi, A.H.M. Nurun
    • BMB Reports
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    • v.36 no.6
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    • pp.565-571
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    • 2003
  • The locomotor responses of human peripheral blood neutrophils and lymphocytes were measured by the change from spherical to polarized shapes in the presence of endotoxins (lipopolysaccharide, LPS) of enteric pathogens: S. dysenteriae type 1, V. cholerae Inaba 569B, S. typhimurium, and K. pneumoniae. We reported earlier that these endotoxins are chemotactic factors for the neutrophils since they stimulated cell polarization within a few minutes of incubation. Endotoxins had an inhibitory effect upon neutrophil phagocytosis of opsonized yeast and the cells engulfed fewer yeasts. Interestingly, endotoxins increased neutrophil adhesion to clean glass surfaces, but stimulated the cells to exhibit increased random locomotion (chemokinesis) through cellulose nitrate filters and show an enhanced ability to reduce nitroblue tetrazolium (NBT) dye. Unlike neutrophils, lymphocytes direct from blood do not show polarized morphology towards chemotactic factors but the cells acquire locomotor capacity during 24-72 h culture with mitogens such as phytohemagglutinin (PHA), phorbol myristate acetate or concanavalin A. Stimulation of blood lymphocytes with endotoxins did not induce cell polarization in short-term but long-term culture resulted in an increase in the proportion of polarized cells that acquired locomotor morphologies. The majority of these cells were identified as esterase negative B-lymphocytes that migrated through filters. Despite the optimum time of incubation for each of these cell types being different, we found that lymphocytes respond to much lower concentrations of endotoxins than the neutrophils. These findings suggest that endotoxins of enteric pathogens modulate the functions of human blood neutrophils and lymphocytes.

Effects of the pH Change of Ash Solution and the Addition of Chitosan on the Caesalpinia sappan Dyeing in the Simultaneous Mordanting with the Addition of Ash Solution (잿물 동시매염에 의한 소목의 염색에서 잿물의 pH 변화와 키토산 첨가가 염색성에 미치는 영향)

  • Seo, Hee-Sung;Jeon, Dong-Won;Kim, Jong-Jun
    • The Research Journal of the Costume Culture
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    • v.13 no.5 s.58
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    • pp.686-698
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    • 2005
  • In this study, simultaneous mordanting method of dyeing in the Caesalpinia sappan dye liquor mixed with the rice straw ash solution was employed. The dyeing effects were compared and reviewed according to the changes of dyeing mechanisms of pre-mordanting and simultaneous mordanting using ash solutions. By the direct addition of the ash solution, the pH of the liquor was abruptly changed. In the case of maintaining the pH value of the ash solution at about 7 or 8, compared to the pre-mordanting, $a^{*}$ values increased and $b^{*}$ values decreased. As a result, it is highly desirable in terms of selective emphasis on the reddish hue. The color development, differentiated from that obtainable by the dyeing of chitosan pre-treated fabric, was obtained due to the effect of the water soluble chitosan component on the dyeing mechanism.

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Direct Examination of the Dietary Preference of the Copepod Calanus helgolandicus Using the Colorimetric Approach

  • Kang, Hyung-Ku;Poulet, Serge;Ju, Se-Jong
    • Ocean Science Journal
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    • v.42 no.3
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    • pp.193-197
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    • 2007
  • The food selectivity of tethered females of the copepod Calanus helgolandicus was examined by using the colorimetric approach. First, feeding behavior of the copepod did not show any differences between the red-color stained with neutral red and non-stained diets using the diatom Coscinodiscus curvatulus. Then, the copepods were fed a mixtures of two diets, the diatom C. curvatulus, stained with neutral red, and the dinoflagellate Gymnodinium sanguineum for $14\sim60$ minutes of feeding duration. The foregut colors of females were examined using a stereo-microscope and a video monitor. The foreguts of animals fed with a high density of diatoms in mixed diets showed a dark red color, whereas those fed with a high density of dinoflagellate in mixed diets were a dark yellow. The results suggest that this species of copepod may not selectively feed either one of the diets used for this study. Their feeding activity may be more likely related to the density of available prey in their environment. Therefore, this quick and easy colorimetric approach could provide very useful information, like the pre-screening procedure before designing and conducting the time-consuming and complex feeding experiments to understand the feeding ecology of copepods.

Measurement of Cross-sectional Temperature Distribution in Micro-scale Gap Fluid Using LIF Technique in Combination with CLSM (LIF 및 CLSM을 결합한 미소 간극 내 유체의 단면 온도 분포 측정 기법)

  • Jeong, Dong-Woon;Lee, Sang-Yong
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.30 no.9 s.252
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    • pp.834-841
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    • 2006
  • In the present wort the Laser-induced Fluorescence (LIF) technique and Confocal Laser Scanning Microscopy (CLSM) have been combined to measure the temperature distribution across a micro-scale liquid layer as a direct and non-invasive method. Only the fluorescent light emitted from a very thin volume around a focal plane can be selectively detected, and it enables us to measure the liquid temperatures even at the close vicinity of the walls. As an experimental verification, a test section consists of two flat plates (for heating and cooling, respectively) separated by about 240 microns was made, and the methanol mixed with a temperature-sensitive dye, Rhodamine B, was filled in the gap between them. The measured temperature distribution across the gap showed good linearity, which is a typical characteristic of conduction heat transfer through a thin liquid layer. In result, the CLSM-LIF technique proposed in the present study was found to be a promising method to measure the local temperatures in the liquid flow field in microfluidic devices.

Modification of Cotton Fiber by Enzymatic Treatment (효소처리에 의한 면직물의 개질)

  • 조민정;김태경;임용진;이상복
    • Textile Coloration and Finishing
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    • v.6 no.3
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    • pp.15-26
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    • 1994
  • Cotton fabrics were treated with the cellulase which is an enzyme to decompose cellulose and its actional mechanism is known. The optimum condition of the cellulase to the cotton fabrics and the weight losses, tensile strengths of the treated cotton fabrics were also obtained. The cellulase performs a specific catalytic action on the ${\beta}-1$, 4-glucosidic bonds of the cellulose molecules and hydrolyzes them. For that reason, the negative surface charges of the cotton fabrics were increased by additional generation. of hyrdoxyl groups. The increased surface charges cause the decrease of dye adsorption by inhibiting the approach of the anions of direct dyes. But, it was overcome by the use of enough amount of salt, it means that sodium ions of the salt neutralize the almost all of negative charges of the cotton fabrics. The improvement of the water absorbency is also due to the increased hydroxyl groups In addition, their handles including the mechanical properties were measured and caculated by KES system which is a measuring apparatus that numerizes and objectificates human's feeling, especially touch. As the results, we knew that KOSH(stiffness) and FUKURAMI(fulness & softness) were decreased and that NUMERI(smoothness) was increased.

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Synthesis and Electrochromism of Fluorane Dyes for Direct Thermal Printing and Pressure-Sensitive Printing (감압 감열 기록용 Fluorane계 색소의 합성과 전기변색성)

  • Kim, Sung Hoon;Bae, Jin Seok;Hwang, Seok Hwan;Song, Han Chul;Gwon, Tae Sun;Doh, Myung Ki
    • Journal of the Korean Chemical Society
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    • v.40 no.3
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    • pp.209-215
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    • 1996
  • Fluorane dyes has been widely used for pressure and heat sensitive recording system as color forming materials. A new method of synthesizing fluorane dye by the Bamberger reaction has been developed. Electorchromic properties of fluorane dyes was discussed. The fading reaction kinetics follow a first-order relationship and resolved in two kinetics of first-order reaction with rate constant $k_1$ and $k_2$.

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Treatment of Textile Wastewater by Membrane-Bioreactor Process (막-생물반응조 공정을 이용한 염색폐수의 처리)

  • 강민수;김성수;황규대;강종림
    • Membrane Journal
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    • v.7 no.4
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    • pp.175-182
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    • 1997
  • Membrane-bioreactor process which combines anaerobic-aerobic bioreactor and membrane process, was used to remove refractory organic susbstances and dye molecule in textile wastewater effectively. Direct feeding of raw feed water to membrane process caused serious fouling on membrane. On the other hand, pretreated feed by bioreactor before the membrane process remarkably reduced the fouling and prolonged the membrane life. Removal efficiency and fouling were more dependent on the material property of the membrane rather than the membrane pore size and structure. Operation mode of hollow fiber membrane module and linear velocity in the hollow fiber influenced the ramoval efficiency and the water flux of the membrane. The combined membrane- bioreactor process was more effective in treating the textile wastewater than each single process.

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MARGINAL LEAKAGE OF CLASS 2 GLASS-IONOMER CEMENT RESTORATIONS NOT INVOLVING OCCLUSAL SURFACE (교합면을 포함하지 않는 2급 글래스 아이오노머 시멘트 수복물의 변연누출)

  • Lee, Young-Ran;Hur, Bock;Lee, Hee-Joo
    • Restorative Dentistry and Endodontics
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    • v.23 no.1
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    • pp.288-295
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    • 1998
  • The microleakage of direct-access Class 2 restorations was evaluated. Cavities were prepared at mesial and distal proximal enamel surfaces of 20 extracted human molars through buccal window. Prepared cavities were filled with Ketac-Fil, Ketac-Silver, and Fuji II LC, following manufacturer's instructions. 4 specimens of each restorations were made through sectioning 1/4 of bucco-lingual length mesio-distally after thermocycling between $5^{\circ}C$ and $60^{\circ}C$ and imerging 0.5% basic-fuchsin dye solution. Leakage at both occlusal and gingival margin of each specimen was scored 0 to 3 with stereomicroscope. The results were as follows: 1. At occlusal margin, leakage in Ketac-Fil group was more than Ketac-Silver and Fuji II LC group (ANOVA p<0.05) and there was no significant difference between Ketac-Silver and Fuji II LC group(p>0.05), and at gingival margin, there was no significant difference amang materials (p>0.05). 2. Occlusal margin leaked more than gingival margin in Ketac-Silver and Fuji II LC group (t-test p<0.05). 3. Leakage was different according to bucco-lingual location in Ketac-Fil and Fuji II LC group, and lingaul specimen exhibited more leakage(ANOVA p<0.05).

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Indium doping induced defect structure evolution and photocatalytic activity of hydrothermally grown small SnO2 nanoparticles

  • Zeferino, Raul Sanchez;Pal, Umapada;Reues, Ma Eunice De Anda;Rosas, Efrain Rubio
    • Advances in nano research
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    • v.7 no.1
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    • pp.13-24
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    • 2019
  • Well-crystalline $SnO_2$ nanoparticles of 4-5 nm size with different In contents were synthesized by hydrothermal process at relatively low temperature and characterized by transmission electron microscopy (TEM), microRaman spectroscopy and photoluminescence (PL) spectroscopy. Indium incorporation in $SnO_2$ lattice is seen to cause a lattice expansion, increasing the average size of the nanoparticles. The fundamental phonon vibration modes of $SnO_2$ lattice suffer a broadening, and surface modes associated to particle size shift gradually with the increase of In content. Incorporation of In drastically enhances the PL emission of $SnO_2$ nanoparticles associated to deep electronic defect levels. Although In incorporation reduces the band gap energy of $SnO_2$ crystallites only marginally, it affects drastically their dye degradation behaviors under UV illumination. While the UV degradation of methylene blue (MB) by undoped $SnO_2$ nanoparticles occurs through the production of intermediate byproducts such as azure A, azure B, and azure C, direct mineralization of MB takes place for In-doped $SnO_2$ nanoparticles.

Direct Synthesis of Au/TiO2/graphene Composites and Their Application for Degradation of Various Organic Dyes (그래파이트로부터 직접 제조한 Au/TiO2/그래핀 복합체와 이를 이용한 염료의 광분해에 관한 연구)

  • Jeong, Gyoung Hwa;Kim, Sang-Wook
    • Applied Chemistry for Engineering
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    • v.31 no.6
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    • pp.607-611
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    • 2020
  • In this research, we synthesized Au/TiO2/graphene composites using ionic surfactants for the exfoliation of graphite layers, directly. In the graphene composite, TiO2 with thin nanosheet shapes was distributed on the graphene surface and Au nanoparticles with less than 10 nm sizes were evenly distributed on the surface of the TiO2 nanosheets. The Au/TiO2/graphene composite was then applied to the photodegradation of various dyes such as methylene blue, methylene orange and rhodamine 6G, and B. Among them, the methylene blue showed the most excellent photodegradation activity (91.6%) while the rhodamine B exhibited 31.0%.