• Korean Journal of Veterinary Research
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• v.60 no.3
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• pp.109-116
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• 2020

This study aimed to develop a semi-nested polymerase chain reaction assay for the direct detection of Mycoplasma synoviae (M. synoviae) from clinical samples using three newly designed oligonucleotide primers specific to the variable lipoprotein haemagglutinin (vlhA) gene and differentiate M. synoviae field strains based on a nucleotide deletion or the insertion of the proline-rich repeat (PRR) region of the vlhA gene. The developed semi-nested polymerase chain reaction (PCR) assay revealed positive results in 12 out of 100 clinical samples collected from chickens showing lameness and joint swelling. Six positive samples were selected randomly for sequencing, and sequence analysis revealed 96.3-100% nucleotide identities compared to the reference sequences. Phylogenetic analysis showed that sequences of the strains in this study were closely related to WVU1853 (Spain), CK.MS.UDL.PK.2014.2 (Pakistan), and F10-2AS (USA) strains, but they were distinct from the M. synoviae-H vaccine strain sequence. M. synoviae obtained from these samples were identified as types A and C with a length of 38 and 32 amino acids, respectively. These results indicated that the specific and sensitive semi-nested PCR could be a useful diagnostic tool for the direct identification of clinical samples, and the sequence analysis of the partial vlhA gene can be useful for typing M. Synoviae.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.34 no.5C
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• pp.555-560
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• 2009

A vehicle radar has been studied to avoid the collision of vehicles. A vehicle radar using direct sequence-ultra wideband (DS-UWB) measures the distance between a vehicle and an objective with a sufficient time margin. Therefore, the receiver should estimate the distance by processing the short UWB pulse rapidly. There have been several investigations for distance measuring based on DS-UWB signals. In this paper, we propose a novel method for rapid measuring processing time. Simulation results show that the proposed method has a shorter processing time than the conventional method.

• Journal of Microbiology and Biotechnology
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• v.34 no.6
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• pp.1322-1327
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• 2024

The accurate and rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) holds significant clinical importance. This work presents a new method for detecting methicillin-resistant Staphylococcus aureus (S. aureus) in clinical samples. The method uses an aptamer-based colorimetric assay that combines a recognizing probe to identify the target and split DNAzyme to amplify the signal, resulting in a highly sensitive and direct analysis of methicillin-resistance. The identification of the PBP2a protein on the membrane of S. aureus in clinical samples leads to the allosterism of the recognizing probe, and thus provides a template for the proximity ligation of split DNAzyme. The proximity ligation of split DNAzyme forms an intact DNAzyme to identify the loop section in the L probe and generates a nicking site to release the loop sequence ("3" and "4" fragments). The "3" and "4" fragments forms an intact sequence to induce the catalytic hairpin assembly, exposing the G-rich section. The released the G-rich sequence of LR probe induces the formation of G-quadruplex-hemin DNAzyme as a colorimetric signal readout. The absorption intensity demonstrated a strong linear association with the logarithm of the S. aureus concentration across a wide range of 5 orders of magnitude dynamic range under the optimized experimental parameters. The limit of detection was calculated to be 23 CFU/ml and the method showed high selectivity for MRSA.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.29 no.8C
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• pp.1025-1033
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• 2004

This paper covers the detection of covert direct sequence spread spectrum signal without the PN(Pseudo Noise) code information. Due to its low probability of interception, the difficulty of spectrum surveillance increases. Detection parameters are the signal existence of given bandwidth, the length of spreading sequence used by transmitter, and the identification of spreading code for detected chip length. The triple correlation function(TCF) value which is one of the higher order statistical signal processing techniques can be used to detect spread spectrum signal without a prior knowledge, but, it has weakness that TCF results depend on the spread data sequence in actual application. This paper proposes the new scheme that not only overcomes the weakness but also presents better performance than the traditional TCF scheme. The performance comparison of conventional TCF with proposed technique shows that the triple correlation estimator(TCE) has better detection capability.

• The Journal of the Acoustical Society of Korea
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• v.9 no.2
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• pp.42-52
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• 1990

A method of measuring impulse response of acoustic system, two kinds of cross-correlation methods (the direct correlation method and the M-sequence modulation correlation method) were applied. According to the direct correlation method, by using stationary random noise source and by calculating the cross-correlation function between the sourece and the output signal, equivalent impulse response can be obtained not being influenced by the back ground noises. By applying this method, the measurement of echo-time patterns in rooms and oblique incident sound absorption characteristics of sound absorbing materials was carried out. In the case of the M-sequence modulation correlation method which was contrived by Aoshima, an intermittent random nosie modulated by M-sequence signal is used as the source signal, and the cross-correlation function between the M-sequence signal and the squared output signal is calculated. According to this method, equivalent energy impulse response (squared impulse response) of te propagation system can be obtained without being influenced by the back ground noises and the air fluctuation caused by wind. As the applcaition of this measuring method to the architectural acoustics, the meaurements of echo-time patterns, reverberation decays and sound pressure lev디 distributions in rooms and sound insulation efficiencies in buildings were carried out. From these experimental studies, it has been found that this M-sequence modulation correlation method is markedly useful especially for the field masurement of sound insulation under high back ground noise condition.

• Clinical and Experimental Pediatrics
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• v.52 no.5
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• pp.603-606
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• 2009

The Pierre Robin sequence (PRS) is the nonrandom association of micrognathia, cleft palate, and glossoptosis, leading to respiratory and feeding difficulties that appear neurogenic rather than mechanical in causation. Genetic determinants are thought to underlie this functional and morphological entity, based on the existence of Mendelian syndromes with PRS. Here, we demonstrate the association of PRS with trisomy 8p due to duplication of a segment as the karyotype 46,XX,dup(8)(p21.3p23.1) and confirm the additional materials as chromosome 8 via whole chromosome paint probes. Our observation supports the hypothesis regarding a genetic basis for nonsyndromic PRS, strengthens the possible genetic association with isolated cleft palate, and provides a candidate PRS locus in chromosomal region 8(p21.3p23.1).

• The Journal of Korean Institute of Communications and Information Sciences
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• v.38A no.5
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• pp.416-425
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• 2013

A hybrid PN code phase acquisition system based on a least-mean-square adaptive filter, interpreted as a channel estimator is proposed and analyzed for direct-sequence spread-spectrum systems under frequency selective Rayleigh fading channels. Closed form expressions are derived for the filter tap weights and detection/false alarm probabilities. Compared to previously proposed systems, the proposed system achieves smaller mean acquisition times, is more robust to the operating signal-to-noise ratio and allows for multiplication free tap weight updates.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.36 no.1A
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• pp.41-48
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• 2011

This paper proposes an efficient code acquisition scheme for direct-sequence spread spectrum (DSSS) based real-time locating systems (RTLS). The proposed scheme employs multiple bit integration to significantly decrease false alarms when no packet signal is transmitted and increase the detection probability when the RTLS tags are excited. The performance of the proposed code acquisition scheme is analyzed and numerically evaluated, and compared with those of single-dwell and traditional double-dwell code acquisition schemes. The simulation results show that the proposed multiple-bit integration scheme outperforms single-dwell and double-dwell schemes.

• International Journal of Fuzzy Logic and Intelligent Systems
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• v.15 no.1
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• pp.20-26
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• 2015

It is very difficult to design an underwater communication system because of multipath, Doppler effects, noise, and attenuation. These factors lead to errors in the communication performance and maximum propagation distance. In this study, we calculate the distance that can be realized using the direct-sequence code division multiple access (DS-CDMA) technique with direct-sequence spread spectrum (DSSS) in an underwater communication system considering only the attenuation and noise. We also compare the estimated and calculated propagation distances obtained for several different scenarios.

• Proceedings of the Acoustical Society of Korea Conference
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• 1998.06c
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• pp.357-360
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• 1998

DS/CDMA system rejected narrow-band interference and additional White Gaussian noise which are occured at multipath, intentional jammer and multiuser to share same bandwidth in mobile communication systems. Because of having not sufficiently obtained processing gain which is related to system performance, they were not effectively suppressed. In this paper, an matched filter channel model using backpropagation neural network based on complex multilayer perceptron is presented for suppressing interference of narrow-band of direct sequence spread spectrum receiver in DS/CDMA mobile communication systems. Recursive least square backpropagation algorithm with backpropagation error is used for fast convergence and better performance in matched filter receiver scheme. According to signal noise ratio and transmission power ratio, computer simulation results show that bit error ratio of matched filter using backpropagation neural network improved than that of RAKE receiver of direct sequence spread spectrum considering of con-channel and narrow-band interference.