• Earthquakes and Structures
• /
• v.5 no.1
• /
• pp.49-65
• /
• 2013

This article presents the statistical characteristics of elastic floor acceleration spectra that represent the peak response demand of non-structural components attached to a nonlinear supporting frame. For this purpose, a set of stiff and flexible general moment resisting frames with periods of 0.3-3.6 sec. are analyzed using forty-nine near-field strong ground motion records. Peak accelerations are derived for each single degree of freedom non-structural component, supported by the above mentioned frames, through a direct-integration time-history analysis. These accelerations are obtained by Floor Acceleration Response Spectrum (FARS) method. They are statistically analyzed in the next step to achieve a better understanding of their height-wise distributions. The factors that affect FARS values are found in the relevant state of the art. Here, they are summarized to evaluate the amplification and/or reduction of FARS values especially when the supporting structures undergo inelastic behavior. The properties of FARS values are studied in three regions: long-period, fundamental-period and short-period. Maximum elastic acceleration response of non-structural component, mounted on inelastic frames, depends on the following factors: inelasticity intensity and modal periods of supporting structure; natural period, damping ratio and location of non-structural component. The FARS values, corresponded to the modal periods of supporting structure, are strongly reduced beyond elastic domain. However, they could be amplified in the transferring period domain between the mentioned modal periods. In the next step, the amplification and/or reduction of FARS values, caused by inelastic behavior of supporting structure, are calculated. A parameter called the response acceleration reduction factor ($R_{acc}$), has been previously used for far-field earthquakes. The feasibility of extending this parameter for near-field motions is focused here, suggested repeatedly in the relevant sources. The nonlinearity of supporting structure is included in ($R_{acc}$) for better estimation of maximum non-structural component absolute acceleration demand, which is ordinarily neglected in the seismic design provisions.

• Korean Journal of Soil Science and Fertilizer
• /
• v.50 no.6
• /
• pp.509-519
• /
• 2017

Crop simulation models are valuable tools for estimating crop yield, environmental factors and management practices. The objective of this study was to evaluate the effect of soil types on barley productivity using CERES (Crop Environment REsource Synthesis)-barley, cropping system model. So the behavior of the model under various soil types and climatic conditions was evaluated. The results of the sensitivity analysis in temperature, $CO_2$, and precipitation showed that soil types had a direct impact on the simulated yield of CERES-barley model. We found that barley yield in clay soils would be more sensitive to precipitation and $CO_2$ in comparison with temperature. And the model showed limited accuracy in simulating water and nitrogen stress index for soil types. In general, the barley grown on clay soils were less sensitive to water stress than those grown on sandy soils. Especially it was found that the CERES model underestimated the effect of water stress in high precipitation which led to overprediction of crop yield in clay soils. In order to solve these problems and successfully forecast grain yield, further studies on the modification of the water stress response of crops should be considered prior to use of the CERES-barley model for yield forecasting.

• Journal of the Korean Recycled Construction Resources Institute
• /
• v.9 no.2
• /
• pp.143-151
• /
• 2021

Flexural test of reinforced concrete beam with corroded reinforcement were performed to measure the deflection, curvature and cracking moment for various bar diameter and amounts of corrosion. The amounts of corrosion are varied from 0% to 10% by weight and the bar diameters are chosen as 10mm, 13mm, and 19mm. The changes in reinforcement diameter do not affect the flexural behaviors significantly according to this experiment. If the amounts of corrosion is greater than 2%, the deflection and curvature of the beam increased and the cracking moment decreased. It means that the lower amounts of corrosion does not result structural damage in flexural member significantly as in direct tensile test. A modification factor considering an effect of amounts of corrosion is proposed based on the experiment, which can be used to determine the deflection of reinforced concrete beam with corroded reinforcement.

• Molecules and Cells
• /
• v.42 no.7
• /
• pp.503-511
• /
• 2019

As sessile organisms, plants have developed sophisticated system to defend themselves against microbial attack. Since plants do not have specialized immune cells, all plant cells appear to have the innate ability to recognize pathogens and turn on an appropriate defense response. The plant innate immune system has two major branches: PAMPs (pathogen associated molecular patterns)-triggered immunity (PTI) and effector-triggered immunity (ETI). The ability to discriminate between self and non-self is a fundamental feature of living organisms, and it is a prerequisite for the activation of plant defenses specific to microbial infection. Arabidopsis cells express receptors that detect extracellular molecules or structures of the microbes, which are called collectively PAMPs and activate PTI. However, nucleotidebinding site leucine-rich repeats (NB-LRR) proteins mediated ETI is induced by direct or indirect recognition of effector molecules encoded by avr genes. In Arabidopsis, plasmamembrane localized multifunctional protein RIN4 (RPM1-interacting protein 4) plays important role in both PTI and ETI. Previous studies have suggested that RIN4 functions as a negative regulator of PTI. In addition, many different bacterial effector proteins modify RIN4 to destabilize plant immunity and several NB-LRR proteins, including RPM1 (resistance to Pseudomonas syringae pv. maculicola 1), RPS2 (resistance to P. syringae 2) guard RIN4. This review summarizes the current studies that have described signaling mechanism of RIN4 function, modification of RIN4 by bacterial effectors and different interacting partner of RIN4 in defense related pathway. In addition, the emerging role of the RIN4 in plant physiology and intercellular signaling as it presents in exosomes will be discussed.

• Mass Spectrometry Letters
• /
• v.10 no.2
• /
• pp.66-70
• /
• 2019

Rivaroxaban (RRN) is the first available active direct factor Xa inhibitor (anticoagulant) with oral administration. Due to its success in market, there have been efforts to develop various RRN formulations, and the development of good analytical methods for its in vivo evaluation is an essential prerequisite. Thus, here, a simple and efficient method to determine RRN in rat plasma using liquid-liquid extraction (LLE) and liquid chromatography and multiple reaction monitoring (LC-MRM) was presented. The use of ethyl acetate as the LLE solvent results appropriate extraction and purification of RRN and it also helps the significant reduction of rat plasma volume required for RRN quantitation. The developed method showed good analytical performance including specificity, linearity ($r^2{\geq}0.999$ within 0.5 - 500 ng/mL), sensitivity (the lower limit of quantitation at 0.5 ng/mL), accuracy (89.3 - 107.0%), precision (${\geq}12.7%$), and recovery (89.2 - 105.7%). Additionally, RRN in sample extracts showed good stability. Finally, the applicability of the validated method to the PK evaluation of RRN was confirmed after its oral administration to normal rats. The present method is the first analytical method employing LLE for the simple and efficient extraction and purification of RRN in rat plasma. Therefore, the present method can contribute to the development of new RRN formulations as well as to the monitoring of RRN in special clinical situations through its efficient determination in various samples with or without minor modification.

• Korean Journal of Chemical Engineering
• /
• v.35 no.11
• /
• pp.2241-2255
• /
• 2018

RSM methodology was applied to present mathematical models for the fabrication of polyvinylidene fluoride (PVDF) dual-layer hollow fibers in membrane distillation process. The design of experiments was used to investigate three main parameters in terms of polymer concentration in both outer and inner layers and the flow rate of dope solutions by the Box-Behnken method. According to obtained results, the optimization was done to present the proper membrane with desirable properties. The characteristics of the optimized membrane (named HF-O) suggested by the Box-Behnken (at the predicted point) showed that the proposed models are strongly valid. Then, a morphology study was done to modify the fiber by a combination of three types of a structure such as macro-void, sponge-like and sharp finger-like. It also improved the hydrophobicity of outer surface from 87 to $113^{\circ}$ and the mean pore size of the inner surface from 108.12 to 560.14 nm. The DCMD flux of modified fiber (named HF-M) enhanced 62% more than HF-O when it was fabricated by considering both of RSM and morphology study results. Finally, HF-M was conducted for long-term desalination process up to 100 hr and showed stable flux and wetting resistance during the test. These stepwise approaches are proposed to easily predict the main properties of PVDF dual-layer hollow fibers by valid models and to effectively modify its structure.

• Journal of Korean Society on Water Environment
• /
• v.38 no.3
• /
• pp.143-149
• /
• 2022

Membrane distillation (MD) has emerged as a sustainable desalination technology to solve the water and energy problems faced by the modern society. In particular, the surface wetting properties of the membrane have been recognized as a key parameter to determine the performance of the MD system. In this study, a novel surface modification technique was developed to induce a Janus-type hydrophilic/hydrophobic layer on the membrane surface. The hydrophilic layer was created on a porous PVDF membrane by vapor phase polymerization of the pyrrole monomer, forming a thin coating of polypyrrole on the membrane walls. A rigid polymeric coating layer was created without compromising the membrane porosity. The hydrophilic coating was then followed by the in-situ growth of siloxane nanoparticles, where the condensation of organosilane provided quick loading of hydrophobic layers on the membrane surface. The composite layers of dual coatings allowed systematic control of the surface wettability of porous membranes. By the virtue of the photothermal property of the hydrophilic polypyrrole layer, the desalination performance of the coated membrane was tested in a solar MD system. The wetting properties of the dual-layer were further evaluated in a direct-contact MD module, exploring the potential of the Janus membrane structure for effective and low-energy desalination.

• Journal of Periodontal and Implant Science
• /
• v.52 no.6
• /
• pp.437-454
• /
• 2022

Embryonic stem cells have been a popular research topic in regenerative medicine owing to their pluripotency and applicability. However, due to the difficulty in harvesting them and their low yield efficiency, advanced cell reprogramming technology has been introduced as an alternative. Dental stem cells have entered the spotlight due to their regenerative potential and their ability to be obtained from biological waste generated after dental treatment. Cell reprogramming, a process of reverting mature somatic cells into stem cells, and transdifferentiation, a direct conversion between different cell types without induction of a pluripotent state, have helped overcome the shortcomings of stem cells and raised interest in their regenerative potential. Furthermore, the potential of these cells to return to their original cell types due to their epigenetic memory has reinforced the need to control the epigenetic background for successful management of cellular differentiation. Herein, we discuss all available sources of dental stem cells, the procedures used to obtain these cells, and their ability to differentiate into the desired cells. We also introduce the concepts of cell reprogramming and transdifferentiation in terms of genetics and epigenetics, including DNA methylation, histone modification, and non-coding RNA. Finally, we discuss a novel therapeutic avenue for using dental-derived cells as stem cells, and explain cell reprogramming and transdifferentiation, which are used in regenerative medicine and tissue engineering.

• Molecules and Cells
• /
• v.46 no.6
• /
• pp.351-359
• /
• 2023

Deamination of adenine or cytosine in RNA, called RNA editing, is a constitutively active and common modification. The primary role of RNA editing is tagging RNA right after its synthesis so that the endogenous RNA is recognized as self and distinguished from exogenous RNA, such as viral RNA. In addition to this primary function, the direct or indirect effects on gene expression can be utilized in cancer where a high level of RNA editing activity persists. This report identified actin-related protein 2/3 complex inhibitor (ARPIN) as a target of ADAR1 in breast cancer cells. Our comparative RNA sequencing analysis in MCF7 cells revealed that the expression of ARPIN was decreased upon ADAR1 depletion with altered editing on its 3'UTR. However, the expression changes of ARPIN were not dependent on 3'UTR editing but relied on three microRNAs acting on ARPIN. As a result, we found that the migration and invasion of cancer cells were profoundly increased by ADAR1 depletion, and this cellular phenotype was reversed by the exogenous ARPIN expression. Altogether, our data suggest that ADAR1 suppresses breast cancer cell mobility via the upregulation of ARPIN.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2021.04a
• /
• pp.36-36
• /
• 2021

Cryopreservation has been broadly used as an efficient method for a long-term conservation for many types of plants especially vegetatively propagated plants. Among several cryopreservation methods, a droplet-vitrification was the most widely applicable and efficient method. Studies have developed protocols for strawberry using droplet-vitrification method and suggested the practical use of the protocol for large number of germplasm with a little modification. In this study, the droplet vitrification method of shoot tip has been tested on 31 accessions provided around the world. Shoot tips were precultured on Murashige and Skoog (MS) liquid medium supplemented with 0.3~0.5M sucrose. Precultured explants were osmoprotected with loading solution, 35% of PVS3 (C4, 17.5% glycerol and 17.5% sucrose) for 40 min and exposed to dehydration solution, PVS3 (B1, 50% glycerol and 50% sucrose) for 60 min. Then, the explants were transferred onto droplets containing 2.5 uL PVS3 on sterilized aluminum foils prior to direct immersion in liquid nitrogen (LN) for 1hr. The cryopreserved shoot tips were rapidly warmed in a water bath at 40C and then unloaded in MS with 0.8M sucrose for 40 min. The shoot tips were cultured in NH₄NO₃-free MS post culture medium for 2 weeks. Subsequently, the explants were moved to the MS medium for 6 weeks and evaluated the regrowth rate. By this droplet-vitrification protocol, twenty-four accessions showed at least 40% regrowth rate. Out of 24 accessions, 'Nonsan1ho' had the highest regeneration rate of 85.8% and 'Jumbo pureberry' had the lowest with 42.1%.