• Analytical Science and Technology
• /
• v.36 no.1
• /
• pp.44-52
• /
• 2023

This protocol clarifies a simple and precise method for measuring the activity of xanthine oxidase (XO) enzyme inhibitor. XO enzyme, which accelerates oxidative stress-related disorders through its capacity to generate hydrogen peroxide and superoxide anion radicals (O₂^•-), has been found to be inhibited by several plant extracts. Enzyme samples were incubated with a suitable buffer containing adequate amounts of xanthine as a substrate to determine XO activity. The method depends on direct measurements of uric acid and hydrogen peroxide production to test XO with and without interference. The CUPRAC reagent (Cu(Nc)₂²⁺) was used to inhibit enzyme reaction after incubation was complete. The generated urate and peroxide reduced the Cu(II)-neocuproine complex (Cu(Nc)₂²⁺) to a brightly colored Cu(I)-neocuproine complex (Cu(Nc)₂⁺), which was assessed with a spectrophotometer at 450 nm. XO activity was found to be directly related to the increased absorbance of the colored Cu(I)-neocuproine complex (Cu(Nc)₂⁺). To eliminate catalase enzyme interference, the proposed method used sodium azide and was validated against XO activity using the UV method in matched samples with t-test analysis. The proposed assay can determine XO activity with high precision, as indicated by the correlation coefficient (R² = 0.9935) from comparison with the reference protocol.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.8 no.5
• /
• pp.1096-1101
• /
• 2004

The 3-D shape use to effect of movie, animation, industrial design, medical treatment service, education, engineering etc.... But it's not easy to make 3-D shape from the information of 2-D image. There are two methods in restoring 3-D video image through 2-D image; First the method of using a laser; Secondly the method of acquiring 3-D image through stereo vision. Instead of doing two methods with many difficulties, I figure out the method of simple 3-D image in this research paper. We present here a simple and efficient method, called direct calibration, which doesn't require any equations at all. The direct calibration procedure builds a lookup table(LUT) linking image and 3-D coordinates by a real 3-D triangulation system. The LUT is built by measuring the image coordinates of a grid of known 3-D points, and recording both image and world coordinates for each point; the depth values of all other visible points are obtained by interpolation.

• The Journal of Asian Finance, Economics and Business
• /
• v.8 no.3
• /
• pp.119-126
• /
• 2021

This study is to examine the foreign direct investment (FDI) response to real effective exchange rate volatility in Vietnam by using the vector autoregression model. The research data are quarterly frequency data in the period from 2004:Q1 to 2019:Q2. The data on real effective exchange rate were collected from the statistics of Bruegel (Europe) and FDI data were collected from the International Financial Statistics. The quantitative study was conducted with two steps: (1) measuring exchange rate volatility by the GARCH(1,1) method; and (2) examining the impact of exchange rate volatility on FDI in the context of the global financial crisis. The estimation results show that FDI responded significantly to real exchange rate volatility with the lag of 3 periods at the 5% significance level. The FDI response increased after the exchange rate volatility with the lag of 3 periods, and the impact extended to the lag of 6 periods, and then gradually stabilized. The research findings indicate that FDI in Vietnam responds positively and significantly to exchange rate volatility with the lag of 3 periods. Simultaneously, the negative impact of the global financial crisis in 2008 with the lag of 2 periods leads to a slight decrease in FDI inflows into Vietnam.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.27 no.5
• /
• pp.120-129
• /
• 2023

Hanger cable tension is a major response that can determine the integrity and safety of suspension bridges. In general, the vibration method is used to estimate hanger cable tension on operational suspension bridges. It measures natural frequencies from hanger cables and indirectly estimates tension using the geometry conditions of the hanger cables. This study estimated the hanger cable tension of the Palyeong Bridge using a vision-based system. The vision-based system used digital camcorders and tripods considering the convenience and economic efficiency of measurement. Measuring the natural frequencies for high-order modes required for the vibration method is difficult because the hanger cable response measured using the vision-based system is displacement-based. Therefore, this study proposed a back analysis technique for estimating tension using the natural frequencies of low-order modes. Optimization for the back analysis technique was performed by defining the difference between the natural frequencies of hanger cables measured in the field and those calculated using finite element analysis as the objective function. The direct search method that does not require the partial derivatives of the objective function was applied as the optimization method. The reliability and accuracy of the back analysis technique were verified by comparing the tension calculated using the method with that estimated using the vibration method. Tension was accurately estimated using the natural frequencies of low-order modes by applying the back analysis technique.

• The Bulletin of The Korean Astronomical Society
• /
• v.38 no.1
• /
• pp.31.1-31.1
• /
• 2013

Barred-spiral galaxies possess double patterns: a bar and spiral arms. While their angular speeds play an important role in governing gas dynamical evolution of barred spiral galaxies, there is no direct way to observe them. The Tremaine-Weinberg (TW) method has been one of the most reliable indirect methods to estimate pattern speeds, although it requires a few strict assumptions, notably one that the gas tracer is in a quasi-steady state. In barred-spiral galaxies, however, non-steady gas flows are significant especially when the double patterns have different angular speeds. Using numerical models, we explore the effect of non-steady gas motions on the determination of double pattern speeds based on the TW method. We find that the TW method is accurate within 15% when there is only a single pattern or when double patterns have the same angular speed. When double patterns have different speeds, on the other hand, neglecting the non-steady flows leads to quite large errors (> 30%) in the derived pattern speeds, and severely underestimate the real values for the viewing angle parallel to the bar minor axis. This suggests that one should be cautious when applying the TW method to galaxies with double patterns with different speeds.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.47 no.1
• /
• pp.9-21
• /
• 2021

In this study, the difference between the preservative efficacy test (PET) used for water dispersion formulations and PCPC (personal care products council) guidelines alternative test method, direct contact membrane method, and surface mold test should be studied to determine what should be considered during preservative efficacy test of water non-dispersible formulations. We conducted improved PETs when using the alternative test method compared with the test methods used for stick and pressed powder. There was no significant improvement between water-in-silicon emulsions and loose powder using alternative test methods. When we checked the results of the presence and absence of the preservative system for each product, we could see that there were differences in testing methods. As a result, improved levels of results could be obtained using both existing and alternative test methods when measuring preservatives for water non-dispersible formulations. In addition, in the case of stick and pressed powder, the results were more effective when the preservative test method applied to the consumer's method of use was applied.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.25 no.7
• /
• pp.1147-1154
• /
• 2001

One of major problems in analyzing failure mechanism of real components is the accurate measurement of crack size and shape. The DCPD(Direct Current Potential Drop) method has been widely used for the crack measurement of a structure and finite element analysis has been used for the derivation of calibration equations, which relates the potential drop with the crack depth. In this paper, finite element analyses were performed for semi-elliptical surface cracks with various crack shapes(a/c) and crack depths(a/t). As a result, a calibration equation has been derived for the measurement of a semi-elliptical surface crack in wide plates. Analytical results are compared with experimental results to evaluate the validity and the applicability of the derived equation. The proposed method is expected to provide efficient and accurate measurement of a surface crack during crack growth.

• Journal of Multimedia Information System
• /
• v.8 no.1
• /
• pp.1-10
• /
• 2021

Heart Rate is a crucial physiological parameter that provides basic information about the state of the human body in the cardiovascular system, as well as in medical diagnostics and fitness assessments. At present day, it has been demonstrated that facial video-based photoplethysmographic signal captured using a low-cost RGB camera is possible to retrieve remote heart rate. Traditional heart rate measurement is mostly obtained by direct contact with the human body, therefore, it can result inconvenient for long-term measurement due to the discomfort that it causes to the subject. In this paper, we propose a non-contact-based remote heart rate measuring approach of the subject which depends on the color intensity variation of the subject's facial skin. The proposed method is applied in two regions of the subject's face, forehead and cheeks. For this, three different algorithms are used to measure the heart rate. i.e., Fast Fourier Transform (FFT), Independent Component Analysis (ICA) and Principal Component Analysis (PCA). The average accuracy for the three algorithms utilizing the proposed method was 89.25% in both regions. It is also noteworthy that the FastICA algorithm showed a higher average accuracy of more than 92% in both regions. The proposed method obtained 1.94% higher average accuracy than the traditional method based on average color value.

• Journal of the Korean Society for Precision Engineering
• /
• v.16 no.6
• /
• pp.52-57
• /
• 1999

Funtional metal prototypes are often required in numerous industrial applications. These components are typically needed in the early stage of a project to determine form, fit and function. Recent R/P(Rapid Prototyping) part are made of soft materials such as plastics, wax, paper, these master models cannot be employed durable test in real harsh working environment. Parts by direct metal rapid tooling method, such as laser sintering, by now are hard to get net shape, pores of the green parts of powder casting method must be infiltrated to get proper strength as tool, and new type of 3D direct tooling system combining fabrication welding arc and cutting process is reported by song etc. But a system which can build directly 3D parts of high performance functional material as metal part would need long period of system development, massive investment and other serious obstacles, such as patent. In this paper, through the rapid tooling process as silicon rubber molding using R/P master model, and fabricate wax pattern in that silicon rubber mold using vacuum casting method, then we tranlsated the wax patterns to numerous metal prototypes by new investment casting process combined conventional investment casting with rapid pototyping & rapid tooling process. with this wax-injection-mold-free investment casting, we developed new investment casting process of fabricating numerous functional metal prototypes from one master model, combined 3-D CAD, R/P and conventional investment casting and tried to expect net shape measuring total dimension shrinkage from R/P part to metal part.

• Journal of Dental Rehabilitation and Applied Science
• /
• v.32 no.3
• /
• pp.158-168
• /
• 2016

This article is to review various methods used to investigate internal and marginal adaptation of fixed dental prostheses, and to summarize a merit, worth, and limitation of each method, using some results of previous studies. The methods of measuring internal and marginal gap are divided into two categories in this study; in vivo and in vitro. In vivo methods are clinical evaluations, including exploration, radiography, and impression technique. In vitro methods are laboratory evaluations such as direct view, cross-sectioning, and silicone replica technique using microscope. Measuring by micro computed tomography (CT) or profilometer is also in vitro methods. In recent years, the development of scanning systems is able to analyze 3-dimensional internal and marginal space in detail. As measuring and analyzing technology become more advanced, the ability to thoroughly examine crown adaptation is becoming both simpler and more efficient.