• International Journal of Fluid Machinery and Systems
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• 제7권2호
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• pp.80-85
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• 2014

In this study, we visualized the internal flow of a regenerative turbomachinery using the direct injection tracer method. For visualization, the working fluid was water and the tracer was oil colors (marbling colors). Droplets were injected at the inlet of the machinery and the streak were recorded using a high-speed camera with high-power light sources. While circulating inside the groove, the droplets were translated by the rotational motion of the impeller. When the droplets flow out of the impeller groove, relative to the impeller, they moved more slowly. And the droplets repeatedly reentered into the groove and circulated again. Then the droplets either flowed to the outlet or to the stripper. As a result, this experiment has confirmed the internal circulating flow of a regenerative turbomachinery.

• 한국유체기계학회 논문집
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• 제16권5호
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• pp.24-28
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• 2013

Visualization of internal flow of a regenerative blower has been made by injecting a tracer directly into the flow. For the convenience of visualization, working fluid has been replaced by water and marbling color oil has been used as a tracer. Oil droplet has been injected near the inlet of the blower and the streak has been recorded using a high speed camera with the illumination of high power light sources. At first, droplets have irregular motion in the near inlet area and enter into a groove of the impeller. Then the droplets circulate inside the groove while translated by the rotational motion of the impeller. When the droplets get out of the impeller groove, their speed is lower than that of impeller. And the droplets repeatedly enter into the groove and circulate inside the grooves. Then the droplets either flow to the outlet or reenter into the inlet area through stripper. Through this experimental study, internally circulating motion of the flow inside a regenerative blower has been characterized.

• 대한약침학회지
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• 제18권3호
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• pp.75-79
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• 2015

Objectives: As the peripheral part of the primo vascular system (PVS) is difficult to visualize, we used a vascular casting material Mercox injected directly into the skin to take advantage of a simple procedure to visualize PVS structures as primo vessels (PVs) and primo nodes (PNs) in the skin. Methods: Two colors of the polymer Mercox were injected into mouse skin. After a partial maceration of the whole body with potassium hydroperoxide solution, we anatomized it under a stereomicroscope to trace the Mercox that had been injected into the PVS. Results: Injection of Mercox directly into the skin allowed the PVs and the PNs to be visualized. This approach can fill the PVS when the material is ejected out of the PVs or PNs. The shapes, sizes, and topographic positions of the nodes and the vessels are the hallmarks used to identify the PVS in skin when Mercox is used as a tracer. Conclusion: The direct injection of the casting material Mercox into skin, with modified partial maceration procedures, is a promising method for visualizing the PVs and the PNs in the peripheral part of the PVS in skin. The polymer Mercox can penetrate through the primo pores of the primo vascular wall and fill the PVs and the PNs. The data prove that PVs and PNs exist on the hypodermal layer of the skin.

• 생약학회지
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• 제11권3_4호통권43호
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• pp.133-140
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• 1980

In order to develop the radio-immunoassay procedure for the ginsenoside $Rg_1$ we prepared the $Rg_1-BSA$ conjugate and $Rg_1-tyramine$ conjugate by condensing the $Rg_1-azide$, which was prepared by a series of six step chemical modification of the $Rg_1-side$ chain, with bovine serum albumin(BSA) or with tyramine. Rabbits were immunized by repeated injection of $Rg_1-BSA$ conjugate with Freund's Complete Adjuvant for 5 month long to obtain very potent $anti-Rg_1$ serum. The radio-labelled haptene was prepared by direct radio-iodination $(125_J)$ of $Rg_1-tyramine$ according to the chloramine-T method. The radio-immunoassay procedure was successfully furnished by using DCC method (dextran coated charcoal) and the anti-body titer of the anti-serum was found as being $1600{\sim}3200$ by using 15000cpm tracer per test. Calibration test using non-labelled $Rg_1$ showed linear competetive binding response in the $(8-300){\times}34pg$. range of non-labelled $Rg_1$. The cross reaction test using 19 ginsenoside analogues enabled us a full structure-activity analysis on the antigen-antibody reaction that the anti-body in the serum would recognize the full structure of ginsenoside $Rg_1$ except the side chain moiety.