• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2021.05a
• /
• pp.141-143
• /
• 2021

The Activity patterns of animal species are difficult to access and the behavior of freely moving individuals can not be assessed by direct observation. As it has become large challenge to understand the activity pattern of animals such as dogs, and cats etc. One approach for monitoring these behaviors is the continuous collection of data by human observers. Therefore, in this study we assess the activity patterns of dog using the wearable sensors data such as accelerometer and gyroscope. A wearable, sensor -based system is suitable for such ends, and it will be able to monitor the dogs in real-time. The basic purpose of this study was to develop a system that can detect the activities based on the accelerometer and gyroscope signals. Therefore, we purpose a method which is based on the data collected from 10 dogs, including different nine breeds of different sizes and ages, and both genders. We applied six different state-of-the-art classifiers such as Random forests (RF), Support vector machine (SVM), Gradient boosting machine (GBM), XGBoost, k-nearest neighbors (KNN), and Decision tree classifier, respectively. The Random Forest showed a good classification result. We achieved an accuracy 86.73% while the detecting the activity.

• Journal of Sensor Science and Technology
• /
• v.32 no.4
• /
• pp.219-222
• /
• 2023

Partial discharges (PD) have long been recognized as a major contributing factor to catastrophic failures in high-power equipment. As the demand for high voltage direct current (HVDC) facilities continues to rise, the significance of on-line and real-time monitoring of PD becomes increasingly prominent. In this study, we have designed, fabricated, and characterized a highly sensitive and cost-effective PD sensor comprising a pair of copper electrodes with different arc lengths. The key advantage of our sensor is its non-invasive nature, as it can be installed at any location along the entire power cable without requiring structural modifications. In contrast, conventional PD sensors are typically limited to installation at cable terminals or insulation joint boxes, often necessitating invasive alterations. Our PD sensor demonstrates exceptional accuracy in estimating PD location, with a success rate exceeding 95% in the straight sections of the power cable and surpassing 89% in curved sections. These remarkable characteristics indicate its high potential for realtime and on-line detection of PD.

• Electronics and Telecommunications Trends
• /
• v.39 no.4
• /
• pp.54-62
• /
• 2024

The emergence and resurgence of novel respiratory infectious diseases since the turn of the millennium, including SARS, H1N1 flu, MERS, and COVID-19, have posed a significant global health threat. Efforts to combat these threats have involved various approaches, however, continued research and development are crucial to prepare for the possibility of emerging viruses and viral variants. Direct detection methods for viral pathogens include molecular diagnostic techniques and immunodiagnostic methods, while indirect diagnostic methods involve detecting changes in the condition of infected patients through imaging diagnostics, gas analysis, and biosignal measurement. Molecular diagnostic techniques, utilizing advanced technologies such as gene editing, are being developed to enable faster detection than traditional PCR methods, and research is underway to improve the efficiency of diagnostic devices. Diagnostic technologies for infectious diseases continue to evolve, and several key trends are expected to emerge in the future. Automation will facilitate widespread adoption of rapid and accurate diagnostics, portable diagnostic devices will enable immediate on-site diagnosis by healthcare professionals, and advancements in AI-based deep learning diagnostic models will enhance diagnostic accuracy.

• Journal of fish pathology
• /
• v.10 no.2
• /
• pp.75-86
• /
• 1997

Solid phase enzyme linked immunosorbent assay (ELISA) was developed to detect the whole cells of Edwardsiella tarda from infected tissues of flounder. Cross-reaction test was performed by ELISA against fish pathogens such as A. hydrophila ATCC7966. V. anguillarum HYUFP5001, Y, ruckeri 11-4, E. ictaluri and Streptococcus sp. NG8206. Rabbit anti-E, tarda Edk-2 sera highly cross-reacted with A. hydrophila ATCC7966 and V. anguillarum HUFP5001. However, the cross-reaction was removed by using the anti-serum pre-adsorbed with A, hydrophila ATCC7966 FKC. The intra-species cross-reaction among E. tarda isolates was very high. ELISA with the whole cell antigens present in tissue homogenate appeared with highly decreased sensitivity, presumably by the co-coating of lipid or proteins in tissues. Thus, it would be necessary to use the infected tissue homogenates diluted more than 100 times with PBS for diagnosis. Interestingly, compared with the using of FKC antigen, the direct detection of viable cells in tissue homogenate showed more sensitive results with detection limit of $1{\times}10^3$ cells/ml in buffer or diluted tissue homogenate. Consequently, the ELISA method developed in this study was specific, rapid and sensitive for diagnosing edwardsiellosis.

• Korean Journal of Microbiology
• /
• v.42 no.1
• /
• pp.19-25
• /
• 2006

This study was undertaken to develop PCR primers for the identification and detection of Streptococcus mutans (by)using species-specific forward and universal reverse primers. These primers targeted the variable regions of the 16S ribosomal RNA coding gene (rDNA). The primer specificity was tested against 11S. mutans strains and 10 different species (22 strains) of oral bacteria. The primer sensitivity was determined by testing serial dilutions of the purified genomic DNA of S. mutans ATCC $25175^T$. The data showed that species-specific amplicons were obtained from all the S. mutans strains tested, which was not observed in the other species. The direct and nested PCR could detect as little as 2 pg and 2 fg of the chromosomal DNA from S. mutans ATCC $25175^T$, respectively. This shows that the PCR primers are highly sensitive and applicable to the detection and identification of S. mutans.

• Journal of KIISE:Software and Applications
• /
• v.29 no.1_2
• /
• pp.114-125
• /
• 2002

In this paper, we are proposing a hierarchical segmentation method that first segments the video data into units of shots by detecting cut and dissolve, and then decides types of camera operations or object movements in each shot. In our previous work[1], each picture group is divided into one of the three detailed categories, Shot(in case of scene change), Move(in case of camera operation or object movement) and Static(in case of almost no change between images), by analysing DC(Direct Current) component of I(Intra) frame. In this process, we have designed two-stage hierarchical neural network with inputs of various multiple features combined. Then, the system detects the accurate shot position, types of camera operations or object movements by searching P(Predicted), B(Bi-directional) frames of the current picture group selectively and hierarchically. Also, the statistical distributions of macro block types in P or B frames are used for the accurate detection of cut position, and another neural network with inputs of macro block types and motion vectors method can reduce the processing time by using only DC coefficients of I frames without decoding and by searching P, B frames selectively and hierarchically. The proposed method classified the picture groups in the accuracy of 93.9-100.0% and the cuts in the accuracy of 96.1-100.0% with three different together is used to detect dissolve, types of camera operations and object movements. The proposed types of video data. Also, it classified the types of camera movements or object movements in the accuracy of 90.13% and 89.28% with two different types of video data.

• Journal of Microbiology and Biotechnology
• /
• v.24 no.2
• /
• pp.144-151
• /
• 2014

Increasing the gene copy number has been commonly used to enhance the protein expression level in the yeast Pichia pastoris. However, this method has been shown to be effective up to a certain gene copy number, and a further increase of gene dosage can result in a decrease of expression level. Evidences indicate the gene dosage effect is product-dependent, which needs to be determined when expressing a new protein. Here, we describe a direct detection of the gene dosage effect on protein secretion through expressing the enhanced green fluorescent protein (EGFP) gene under the direction of the ${\alpha}$-factor preprosequence in a panel of yeast clones carrying increasing copies of the EGFP gene (from one to six copies). Directly examined under fluorescence microscopy, we found relatively lower levels of EGFP were secreted into the culture medium at one copy and two copies, substantial improvement of secretion appeared at three copies, plateau happened at four and five copies, and an apparent decrease of secretion happened at six copies. The secretion of EGFP being limiting at four and five copies was due to abundant intracellular accumulation of proteins, observed from the fluorescence image of yeast and confirmed by western blotting, which significantly activated the unfolded protein response indicated by the up-regulation of the BiP (the KAR2 gene product) and the protein disulfide isomerase. This study implies that tagging a reporter like GFP to a specific protein would facilitate a direct and rapid determination of the optimal gene copy number for high-yield expression.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.32 no.11A
• /
• pp.1086-1095
• /
• 2007

This paper proposes a novel non-coherent TOA estimation scheme using multiple correlation process on the existence of multiple simultaneously operating piconets (SOPs). Impulse radio-ultra wideband (IR-UWB) based on direct sequence spread spectrum (DSSS) using Gold sequence is employed in order to discriminate each piconet. In order to enhance the characteristic of correlation, this paper presents the method of multiple mask operation (MMO). The time of arrival (TOA) of direct line of sight (DLOS) path is estimated via two step coarse/fine timing detection. To verify the performance of proposed scheme, two distinct channel models approved by IEEE 802.15.4a Task Group (TG) are considered. According to the simulation results, it could conclude that the proposed scheme have performed better performance than the conventional method well even in densed indoor multi-path environment as well as in the existence of multiple SOPs.

• Journal of Microbiology and Biotechnology
• /
• v.14 no.2
• /
• pp.268-275
• /
• 2004

To investigate the potential application of the single-cell gel electrophoresis (SCGE) assay to carp as an aquatic pollution monitoring technique, gill, liver, and blood cells were isolated from carp exposed to a direct-acting mutagen, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), or indirect mutagen, $benzo[\alpha]pyrene$ $(B[\alpha]P)$, then the DNA strand breakage was analyzed using the assay. Based on testing 5 different cell isolation methods and 6 electrophoretic conditions, the optimized assay conditions were found to be cell isolation by filter pressing and electrophoresis at a lower voltage and longer running time (at 0.4 V/cm for 40 min). In preliminary experiments, gill and liver cells isolated from carp exposed to MNNG in vitro exhibited DNA damage signals even with 0.5 ppb exposure, which is a much higher dose than previously reported. In the gill cells isolated from carp exposed to 0.01-0.5 ppm MNNG in vivo, significant dose-and time-dependent increases were observed in the tail for 4 days. As such, the linear correlation between the relative damage index (RDI) values and time for each dose based on the initial 48-h exposure appeared to provide effective criteria for the genotoxicity monitoring of direct-acting mutagenic pollution. In contrast, the in vivo exposure of carp to 0.25-1.0 ppm of $B[\alpha]P$ for 7 days resulted in dose-and time-dependent responses in the liver cells, in which 24-h delayed responses for metabolizing activation and gradual repair after 48 h were also observed. Thus, the negative-sloped linear correlation between the RDI and time at each dose based on the initial 48 h appeared to provide more effective criteria for the genotoxicity monitoring of indirect mutagenic pollution.

• Progress in Superconductivity
• /
• v.13 no.1
• /
• pp.1-6
• /
• 2011

Measurement of magnetic signals generated from electric activity of myocardium provides useful information for the functional diagnosis of heart diseases. Key technical component of the magnetocardiography (MCG) technology is SQUID. To measure MCG signals with high signal-to-noise ratio, sensitive SQUID magnetic field sensors are needed. Present magnetic field sensors based on Nb SQUIDs have field sensitivity good enough to measure most of MCG signals. However, for accurate measurement of fine signal pattern or detection of local atrial fibrillation signals, we may need higher field sensitivity. In addition to field sensitivity, economic aspect of the SQUID system is also important. To simplify the SQUID readout electronics, the output voltage or flux-to-voltage transfer of SQUID should be large enough so that direct measurement of SQUID output can be done using room-temperature preamplifiers. Double relaxation oscillation SQUID (DROS), having about 10 times larger flux-to-voltage transfers than those of DC-SQUIDs, was shown to be a good choice to make the electronics compact. For effective cancellation of external noise inside a thin economic shielded room, first-order axial gradiometer with high balance, simple structure and long-baseline is needed. We developed a technology to make the axial gradiometer compact using direct bonding of superconductive wires between pickup coil and input coil. Conventional insert has mechanical support to hold the gradiometer array, and the dewar neck has equal diameter with the dewar bottom. Boiling of the liquid He can generate mechanical vibrations in the gradiometer array due to mechanical connection structure. Elimination of the mechanical support, and direct mounting of the gradiometer array into the dewar bottom can reduce the dewar neck diameter, resulting in the reduction of liquid He consumption.