• Journal of Microbiology
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• v.44 no.2
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• pp.162-170
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• 2006

Oysters are known to be carriers of food-born diseases, but research on viruses in Korean oysters is scarce despite its importance for public health. We therefore tested oysters cultivated in Goheung, Seosan, Chungmu, and Tongyeong, for viral contamination using cell culture and integrated cell culture PCR (ICC-PCR) with Buffalo green monkey kidney (BGMK) and human lung epithelial (A549) cells. Additional screens via PCR, amplifying viral nucleic acids extracted from oysters supplemented our analysis. Our methods found 23.6 %, 50.9 %, and 89.1 % of all oysters to be positive for adenoviruses when cell culture, ICC-PCR, and direct PCR, respectively, was used to conduct the screen. The same methodology identified enteroviruses in 5.45%, 30.9%, and 10.9% of all cases. Most of the detected enteroviruses (81.3%) were similar to poliovirus type 1; the remainder resembled coxsackievirus type A1. A homology search with the adenoviral sequences revealed similarities to adenovirus subgenera C (type 2, 5, and 6), D (type 44), and F (enteric type 40 and 41). Adenovirus-positive samples were more abundant in A549 cells (47.3%) than in BGMK cells (18.2 %), while the reverse was true for enteroviruses (21.8 % vs. 14.5 %). Our data demonstrate that Korean oysters are heavily contaminated with enteric viruses, which is readily detectable via ICC-PCR using a combination of A549 and BGMK cells.

• Bulletin of the Korean Chemical Society
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• v.30 no.2
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• pp.454-458
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• 2009

Polypyrrole (PPy) nanotubes have been synthesized by chemical oxidative polymerization of pyrrole within the pores of polycarbonate membrane using the technology of diffusion of solutes. The nanotubes array prepared by the proposed method can be considered as nanoelectrode ensembles (NEEs). An amperometric uric acid sensor based on PPy NEEs has been developed and used for determination of uric acid in human serum samples. The electrode can direct response to uric acid at potential of 0.60V vs. SCE with wide linear range of $1.52{\times}10^{-6}\;to\;1.54{\times}10^{-3}\;M.\;The\;detection\;limit\;is \;3.02{\times}10^{-7}$ M. This sensor has been used to determine uric acid in real serum samples. PPy NEEs is thought of as a good application in the foreground.

• Proceedings of the KIEE Conference
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• 2006.10a
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• pp.93-94
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• 2006

In this research, the enhancement of electron-transfer activity of hemoglobin (Hb) in dodecanoic acid film was investigated for the first time. This type of composite film was made on glassy carbon electrode by casting method. Cyclic voltammetric result of the modified electrode displays a well defined redox peaks which was attributed to the direct electrochemical response of Rb. Our results illustrate that Rb exchange electrons directly with electrode and exhibits the characteristics of peroxidase. When we apply this modified electrode as a biosensor, it gives excellent performances in the electrocatalytic reduction of hydrogen peroxide ($H_{2}O_{2}$). Through the optimal conditions, the proposed biosensor shows the linear range for H2O2 determination was from $1{\times}10^{-5}$ to $1.25{\times}10^{-4}mol/L$ with a detection limit of $1{\times}10^{-7}mol/L$. The biosensor retained more than 90% of the initial response after 14 days.

• Journal of Aquaculture
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• v.10 no.4
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• pp.403-407
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• 1997

Intraspecific sequence variation was detected by polymerase chain reaction (PCR) and direct sequencing of a 350-nucleotide region of the mitochondrial 12S rRNA gene of two natural populations (Han River and Nakdong River) and one hatchery stock (Jinhae Inland Fisheries Institute) of local strain common carp, one Israeli strain of common carp stock from Pukyong National University (PKU), and one hybrid between Israeli strain of common carp female and local strain common carp male from PKU stock. There is little variation in 350 bases of the mitochondrial 12S rRNA gene sequences among 2 natural and 1 hatchery local strain common carp populatins, representing abut 7 to 20 nucleotide differences (less than 6%). The sequence of specimens from Han River was more similar to that from Nakdong River (identity=98.0%) than to that from Jinhae Inland Fisheries Institute (identity=96.3%). Sequence variation between Israeli strain and wild local strain common carp was higher than the variation within natural stocks. The level of variation was ranged from 15.7 to 17.7%. The hybrid showed very similar nucleotide4 sequence of 12S rRNA gene to the sequence of Israeli strain with the identity of 98.9%.

• Journal of the Korean Society of Combustion
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• v.9 no.3
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• pp.27-35
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• 2004

Diode laser absorption sensors are advantageous because they may provide fast, sensitive, absolute, and selective measurements of species concentration. These systems are very attractive for practical applications owing to its compactness, resonable cost, robustness, and ease of use. In addition, diode lasers are fiber-optic compatible and thus enable simultaneous measurements of multiple species along a line-of-sight. Recent advances of room-temperature, near-IR and visible diode laser sources for telecommunication, optical data storage applications make it possible to be applied for combustion diagnostics based on diode laser absorption spectroscopy. Therefore, combined with fiber-optics and high sensitive detection strategies, compact and portable sensor systems are now appearing for variety of applications. The objectives of this research are to develope a new gas sensing system and to verify feasibility of this system. Wavelength and power characteristics as a function of injection current and temperature are experimentally found out. Direct absorption spectroscopy has been demonstrated in these experiments and has a bright prospect to this diode laser system.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.35 no.8C
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• pp.697-708
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• 2010

In this paper, we compare the timing synchronization performance of the adaptive filter to that of the correlator in direct sequence spread spectrum (DS/SS) systems. The test variables used in the code synchronization are statistically analyzed for both schemes, and then the obtained results are used in calculating the detection and false alarm probabilities. Based on the derived probabilities, the synchronization performance is compared and the simulation is followed. Analysis and simulation results show that the correlator outperforms the adaptive filter under most synchronization environments.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2014.05a
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• pp.152-155
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• 2014

This paper describes a design of digital PWM controller for automotive brake solenoid valve which is used in ABS/ESC SoC. The PWM controller which consists of 12 channels supports both direct control and PI control. Six channels in direct control mode make consistent PWM signals according to duty ratio setting, and the others in PI control mode make PWM signals with constant current depending on current setting. The PWM controller also has functions including solenoid valve open detection, Dither and PWM phase shift. The PWM controller having 44,779 gates is fabricated using a 0.18um CMOS process, and test results show that all the functions are correct.

• Journal of Sensor Science and Technology
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• v.32 no.3
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• pp.199-206
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• 2023

This study presents a novel monitoring technique for underwater high-voltage direct current (HVDC) cables based on the Distributed Acoustic Sensor (DAS). The proposed technique utilizes vibration and acoustic signals generated on HVDC cables to monitor their condition and detect events such as earthquakes, shipments, tidal currents, and construction activities. To implement the monitoring system, a DAS based on phase-sensitive optical time-domain reflectometry (Φ-OTDR) system was designed, fabricated, and validated for performance. For the HVDC cable monitoring experiments, a testbed was constructed on land, mimicking the cable burial method and protective equipment used underwater. Defined various scenarios that could cause cable damage and conducted experiments accordingly. The developed DAS system achieved a maximum measurement distance of 50 km, a distance measurement interval of 2 m, and a measurement repetition rate of 1 kHz. Extensive experiments conducted on HVDC cables and protective facilities demonstrated the practical potential of the DAS system for monitoring underwater and underground areas.

• Journal of the Korean Institute of Gas
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• v.20 no.5
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• pp.72-81
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• 2016

When the coatings of buried steel pipelines are damaged, corrosion could be occurred on the surface of the damaged areas. Moreover the pinhole occurred by corrosion of pipelines may cause accidents due to gas leakage. To prevent these accidents, foreign countries including UK and USA have carried out coating defect detection on the buried gas pipelines by using a DCVG or a ACVG and have conducted direct assessment of pipelines through digging the ground, and if necessary, have repaired the pipelines. That is called ECDA i.e External Corrosion Direct Assessment which is regulated by NACE standards(SP 0502) and etc. In Korea, the ECDA provisions were included in KGS FS551 in 2014 when the regulations of Safety Validation in Detail for the medium-pressure piping were introduced. We have developed the equipment which can be used to detect external corrosion of the buried gas pipelines. We have also constructed pipeline test bed for empirical test of the developed equipment. In addition, we have carried out the verification experiments of the developed equipment on the test bed to demonstrate the performance of the equipment. The experiments were conducted by comparison tests of the developed equipment and other equipments which have been introduced and used in Korea. As the result, we have found the developed equipment is easier to use and has far superior performance compared to other equipment being used in Korea.

• The Journal of Korean Society of Virology
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• v.29 no.4
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• pp.271-281
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• 1999

The nested polymerase chain reaction (PCR) assay was used to determine the sequences of reverse transcriptase (RT) codons 41, 67, 70, 210, 215 and 219 of human immunodeficiency virus-1 (HIV-1) pol gene. Template DNA was obtained from uncultured peripheral blood mononuclear cells from 27 Korean HIV-1 infected patients treated with ZDV and Korean red ginseng. The second PCRs were done for 2 separated regions (RT codons $13{\sim}98$ and $152{\sim}259$) with $5\;{\mu}l$ of the first PCR productNucleotide sequences were determined by direct sequencing. In the 27 patients, CD4+ cell count decreased from $230{\pm}117/{\mu}l$ to $152{\pm}162/{\mu}l$ for $46{\pm}26$ months (Mo), and actual duration of ZDV intake was $72{\pm}16$ Mo. In the 16 patients who had been treated with ZDV therapy ${\ge}25$ Mo, the incidences of 70R, 215F/Y, and 41L were 61%, 28% and 22%, respectively and those of 67N, 210W and 219Q were 17%. The incidences of 215F/Y were 6.7% for group ${\le}12$ Mo treatment, 22.7% for group with 13 to 24 Mo, and 27.8% for group ${\ge}25$ Mo. There was no mutation in 9 patients. It might be associated with the interruption of ZDV therapy for more than 6 months in 6 patients. This study shows that the detection of mutation could be useful prognostic marker with other clinical and virological data, and very low mutation rate is dectected compared to overseas reports.