• KIEE International Transactions on Power Engineering
• /
• 제3A권4호
• /
• pp.214-221
• /
• 2003

In view of the fact that gas insulated switchgear (GIS) is an important piece of equipment in a substation, it is highly desirable to continuously monitor the state of equipment by measuring the partial discharge (PD) activity in a GIS, as PD is a symptom of an insulation weakness/breakdown. However, since the PD signal is relatively weak and the external noise makes detection of the PD signal difficult, it therefore requires careful attention in its detection. In this paper, the algorithm for detection of PD in the GIS using the wavelet transform (WT) is proposed. The WT provides a direct quantitative measure of the spectral content and dynamic spectrum in the time-frequency domain. The most appropriate mother wavelet for this application is the Daubechies 4 (db4) wavelet. 'db4', the most commonly applied mother wavelet in the power quality analysis, is very well suited to detecting high frequency signals of very short duration, such as those associated with the PD phenomenon. The proposed algorithm is based on utilizing the absolute sum value of coefficients, which are a combination of D1 (Detail 1) and D2 (Detail 2) in multiresolution signal decomposition (MSD) based on WT after noise elimination and normalization.

• BMB Reports
• /
• 제37권5호
• /
• pp.546-551
• /
• 2004

The increasing pace of development in molecular biology during the last decade has had a direct effect on mass testing and diagnostic applications, including blood screening. We report the model Microarray that has been developed for Hepatitis B virus (HBV) and Hepatitis D virus (HDV) detection. The specific primer pairs of PCR were designed using the Primer Premier 5.00 program according to the conserved regions of HBV and HDV. PCR fragments were purified and cloned into pMD18-T vectors. The recombinant plasmids were extracted from positive clones and the target gene fragments were sequenced. The DNA microarray was prepared by robotically spotting PCR products onto the surface of glass slides. Sequences were aligned, and the results obtained showed that the products of PCR amplification were the required specific gene fragments of HBV, and HDV. Samples were labeled by Restriction Display PCR (RD-PCR). Gene chip hybridizing signals showed that the specificity and sensitivity required for HBV and HDV detection were satisfied. Using PCR amplified products to construct gene chips for the simultaneous clinical diagnosis of HBV and HDV resulted in a quick, simple, and effective method. We conclude that the DNA microarray assay system might be useful as a diagnostic technique in the clinical laboratory. Further applications of RD-PCR for the sample labeling could speed up microarray multi-virus detection.

• 한국멀티미디어학회논문지
• /
• 제20권11호
• /
• pp.1759-1767
• /
• 2017

Although Open API has been invigorated by advancements in the software industry, diverse types of malicious code have also increased. Thus, many studies have been carried out to discriminate the behaviors of malicious code based on API data, and to determine whether malicious code is included in a specific executable file. Existing methods detect malicious code by analyzing signature data, which requires a long time to detect mutated malicious code and has a high false detection rate. Accordingly, in this paper, we propose a method that analyzes and detects malicious code using association rule mining and an Naive Bayes classification. The proposed method reduces the false detection rate by mining the rules of malicious and normal code APIs in the PE file and grouping patterns using the DHP(Direct Hashing and Pruning) algorithm, and classifies malicious and normal files using the Naive Bayes.

• Journal of Microbiology and Biotechnology
• /
• 제20권3호
• /
• pp.569-573
• /
• 2010

A quantitative detection method for Salmonella in seafood was developed using a SYBR Green-based real-time PCR assay. The assay was developed using pure Salmonella DNA at different dilution levels [i.e., 1,000 to 2 genome equivalents (GE)]. The sensitivity of the real-time assay for Salmonella in seeded seafood samples was determined, and the minimum detection level was 20 CFU/g, whereas a detection level of 2 CFU/ml was obtained for pure culture in water with an efficiency of ${\geq}85%$. The real-time assay was evaluated in repeated experiments with seeded seafood samples and the regression coefficient ($R^2$) values were calculated. The performance of the real-time assay was further assessed with naturally contaminated seafood samples, where 4 out of 9 seafood samples tested positive for Salmonella and harbored cells <100 GE/g, which were not detected by direct plating on Salmonella Chromagar media. Thus, the method developed here will be useful for the rapid quantification of Salmonella in seafood, as the assay can be completed within 2-3 h. In addition, with the ability to detect a low number of Salmonella cells in seafood, this proposed method can be used to generate quantitative data on Salmonella in seafood, facilitating the implementation of control measures for Salmonella contamination in seafood at harvest and post-harvest levels.

• 한국통신학회논문지
• /
• 제29권10A호
• /
• pp.1159-1166
• /
• 2004

이 논문은 페이딩 채널에서 직접확산 부호분할다중접속(DS-CDMA) 시스템을 위한 선형제약 변형 최소평균자 승오차(MMSE) 검출의 이전 연구에 대한 후속 연구이다. 변형된 MMSE 검출과 파일로트 심볼의 도움을 받는 채널추정(PSACE)의 결합시 발생하는 시스템 동작불능을 방지하는 조건을 구하였다. 선형제약 변형 MMSE의 해가 레일레이 페이딩에서의 시간변화에 대해 이론적으로 강건함을 보였다. 이 사실은 시뮬레이션 결과와 일치한다. 아울러 어떤 조건에서 선형제약 변형 MMSE 검출이 출력 신호대간섭-잡음비(SINR)를 최대화하였다.

• 전기학회논문지
• /
• 제67권12호
• /
• pp.1671-1677
• /
• 2018

In this research, the collision detection system based on insect's visual interneuron has been designed. The lobula giant movement detector (LGMD) corresponds to the movement value that increases in direct collision process. If the collision is detected by the LGMD only, it could generate a crash warning even in a non-collision situation, resulting in a lot of false alarms. Directionally sensitive movement detectors (DSMD) are directionally sensitive algorithm based on the elementary movement detectors (EMD) in four directions (up, down, left, and right). In this paper, we propose an efficient VLSI architecture for a realtime collision detection system that is robust to the surrounding environment while improving accuracy. The proposed architecture is synthesized with Dongbu Hightech 110nm standard cell library and shows 333MHz of maximum operating frequency and requires 8400 gates with about 16.5KB of internal memories.

• Journal of Microbiology and Biotechnology
• /
• 제34권3호
• /
• pp.681-688
• /
• 2024

The accurate and rapid detection of methicillin-resistance of Staphylococcus aureus (SA) holds significant clinical importance. However, the methicillin-resistance detection strategies commonly require complicated cell lysis and gene extraction. Herein, we devised a novel colorimetric approach for the sensitive and accurate identification of methicillin-resistance of SA by combining allosteric probe-based target recognition with self-primer elongation-based target recycling. The PBP2a aptamer in the allosteric probe successfully identified the target MRSA, leading to the initiation of self-primer elongation based-cascade signal amplification. The peroxidase-like hemin/G-quadruplex undergo an isothermal autonomous process that effectively catalyzes the oxidation of ABTS2- and produces a distinct blue color, enabling the visual identification of MRSA at low concentrations. The method offers a shorter duration for bacteria cultivation compared to traditional susceptibility testing methods, as well as simplified manual procedures for gene analysis. The overall amplification time for this test is 60 min, and it has a detection limit of 3 CFU/ml. In addition, the approach has exceptional selectivity and reproducibility, demonstrating commendable performance when tested with real samples. Due to its advantages, this colorimetric assay exhibits considerable potential for integration into a sensor kit, thereby offering a viable and convenient alternative for the prompt and on-site detection of MRSA in patients with skin and soft tissue infections.

• 한국염색가공학회지
• /
• 제21권5호
• /
• pp.51-57
• /
• 2009

Since heavy metal pollution is a significant global environmental problem and very dangerous to human health, the improved methods for detecting heavy metals are required recently. Colorimetric chemosensors are now considered as one of the most effective analytical method used in the environment monitoring. New direct dyes having the function of colorimetric chemosensors were synthesized. When metal ions such as $Al^{3+}$, $Ca^{2+}$, $Cd^{2+}$, $Cr^{3+}$, $Cu^{2+}$, $Fe^{2+}$, $Fe^{3+}$, $Hg^{2+}$, $Li^+$, $Mg^{2+}$, $Na^+$, $Ni^{2+}$, $Pb^{2+}$ and $Zn^{2+}$ were added each solution of new direct dyes, the color of solution was changed and can be easily detected with naked eyes without expensive experimental equipment such as atomic absorption spectrometer (AAS) or inductively coupled plasma?mass spectrometer (ICP-MS). The new benzidine analogues were diazotized and reacted with couplers such as H-acid, J-acid, Chromotropic acid, Nevill-winther acid and gamma acid to synthesize new direct dyes. The structures of the new direct dyes were confirmed by high resolution mass spectrometer (FAB ionization) and evaluated with UV-Vis spectroscopy. The UV-VIS spectroscopy was measured for the dye solutions by adding various concentrations of metal ions. It was observed that the absorbance in UV-Vis spectra was changed as the heavy metal ions were added.

• 한국전산구조공학회논문집
• /
• 제25권1호
• /
• pp.91-99
• /
• 2012

본 연구에서는 PZT 소자의 정압전 효과에 의한 동적 응답신호를 이용하는 보 구조물 손상 모니터링 기법을 제안하였다. 특히, 모드 변형에너지기반 보 구조물 손상 모니터링에 PZT 정압전 응답신호를 입력자료로 활용하는 방안에 대한 연구에 주안점이 있다. 먼저, PZT 소자의 정압전 효과 및 동적 변형률 응답의 이론적 배경을 요약하였다. 다음으로, 모드 변형에너지기반 보 구조물 손상위치 모니터링 기법을 제시하였다. 제시된 기법의 적합성을 검증하기 위해, 캔틸레버 보 모형을 대상으로 강제진동 실험을 수행하였으며, 세 종류의 센서(가속도계, PZT 센서, 변형률계)를 통해 동적 응답신호가 계측되었다. 손상 전후에 계측된 이들 진동신호들을 사용하여 모드 변형에너지기반의 손상위치 모니터링이 수행되었다.

• 한국가스학회지
• /
• 제18권5호
• /
• pp.12-19
• /
• 2014

매설배관의 부식위험성을 최소화하고 음극방식의 효율극대화를 위해 다양한 비굴착 간접검사기법들이 수십년 전부터 이용되고 있으며, 미국에서는 굴착을 통한 매설배관 외면부식 직접평가법을 CFR 코드에서 규정하고 있다. 국내의 경우에는 도시가스사업법 관련 4개의 기준(KGS Code)에서 배관의 손상여부를 측정할 수 있는 장비를 이용하여 배관의 상태를 점검, 측정하고 이상부위에 대하여 누출검사를 한 경우 매설배관의 기밀시험을 한 것으로 보고 있을 뿐 배관외면부식 직접평가에 대한 규정은 없다. 본 논문에서는 미국, 영국 등 국외의 매설배관 건전성 관리 기준 및 방법을 조사하고, 국내의 매설배관 방식관리 실태 및 피복손상탐지 장치 이용실태 등을 조사한 후 국내 실정에 맞는 매설배관 외면부식 직접평가법을 제시하였다. 본 논문에서 제시한 매설배관 외면부식 직접 평가법은 국내에서 중압배관의 정밀안전진단 제도를 도입하는데 기초 자료로 활용되었다.