• Journal of Plant Biotechnology
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• v.35 no.3
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• pp.185-193
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• 2008

We evaluated the response to salt stress of two different ginseng lines, STG3134 and STG3159, which are sensitive and tolerant, respectively, to salt treatment. Plants were exposed to a 5 dS/m salt solution, and chlorophyll fluorescence was measured. STG3134 ginseng was more sensitive than STG3159 to salt stress. To characterize the cellular response to salt stress in the two different lines, changes in protein expression were investigated using a proteomic approach. Total protein was extracted from detached salt-treated leaves of STG3134 and STG3159 ginseng, and then separated by two-dimensional polyacrylamide gel electrophoresis(2-DE). Approximately 468 protein spots were detected by 2-DE and Coommassie brilliant blue staining. Twenty-two proteins were found to be reproducibly up- or down-regulated in response to salt stress. Among these proteins, twelve were identified using MALDI-TOF MS and ESI-Q-TOF and classified into several functional groups: photosynthesis-related proteins(oxygen-evolving enhancer proteins 1 and 2, rubisco and rubisco activase), detoxification proteins(polyphenol oxidase) and defense proteins($\beta$-1,3-glucanase, ribonuclease-like storage protein, and isoflavone reductase-like protein). The protein levels of ribonuclease-like storage protein, which was highly induced in STG3159 ginseng as compared to STG3134, correlated tightly with mRNA transcript levels, as assessed by reverse-transcription(RT)-PCR. Our results indicate that salinity induces changes in the expression levels of specific proteins in the leaves of ginseng plants. These changes may, in turn, playa role in plant adaptation to saline conditions.

• Journal of the korean academy of Pediatric Dentistry
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• v.30 no.3
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• pp.406-414
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• 2003

Chemomechanical approach to caries removal was introduced in order to preserve the maximum amount of sound tooth structure. The efficacy of chemomechanical caries removal was assessed using microcomputed tomography which offers 3 dimensional data without destroying the tooth, and the V works program. In group 1, the density values of the sound dentin, carious dentin, and remaining dentin after chemomechanical treatment were analyzed. In group 2, the density values of the sound dentin, cavity wall prepared using high speed bur, and the remaining dentin after additional $Carisolv^{TM}$ gel application on the same cavity were analyzed. The results were as follows; 1. The density value of the remaining dentin after the $Carisolv^{TM}$ treatment was 81.8% of the sound dentin(p < 0.001). 2. The density value of the remaining dentin after the conventional rotary instrument showed no statistically significant difference from that of the sound dentin(p = 0.234).

• Journal of the Korean Geotechnical Society
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• v.21 no.3
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• pp.159-168
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• 2005

In the previous studies, settlement behaviors of granular compaction piles have generally been analyzed with an evaluation of the settlement reduction factor based on tile load-sharing ratio and the replacement ratio. In this approach, however, since the reinforced ground with granular compaction piles is simplified as the composite ground, only the difference of a relative vertical strength between piles and soils is taken into account without reflecting lateral behaviors of granular compaction piles. In the companion research paper, the method of estimating the settlement of granular compaction piles was proposed by synthetically considering a vertical strength of the ground, lateral behaviors of granular compaction piles, the strength of pile materials, a pile diameter, and an installation distance of the pile. In the presented study, to validate a propriety of the previously proposed method, large scale field load tests and three dimensional numerical analyses are performed. The results are analyzed in detail and compared with the predicted settlements by the proposed method. Finally, a simple method to estimate the settlement of granular compaction piles is suggested for an easy application of the practical design.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.33 no.5
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• pp.1777-1783
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• 2013

Parshall flume is more practical one of hydraulic structures for measuring flowrate in open channels and also has more advantages when the magnitude of flow velocity is relatively lower or much more sediments are brought from upstream. International Organization for Standardization (ISO) has suggested the empirical formulas standardized by the sizes and dimensions of Parshall flume. However, the related studies using the numerical simulations and experiments are relatively rare. Therefore, in this study, it was examined whether the numerical simulation was adequacy for reproducing the hydraulic characteristics of Parshall flume as much as laboratory experiments by comparing the results from numerical simulations and empirical equation. And for arbitrary Parshall flume, that is unlisted in the ISO standards due to environmental conditions, constructional difficulties etc, thus, the hydraulic experiments should be conducted to obtain the empirical formulas for it, the results from numerical simulations were compared with those of laboratory experiments. Consequently, it was convinced that the numerical simulation about Parshall flume was simulated appropriately instead of experimental approach. And the dimensionless discharge equation of arbitrary ones was suggested using the results of numerical simulations, and the equation was validated by comparing with laboratory experimental results showing the maximum relative error of 2.3%. If the actual topography, the shape of inlet and submerged flow, which is excluded in this study, were carefully considered, it would be possible to supply a simple empirical discharge equation based on numerical results. Also, it can replete hard works for hydraulic experiment being error-prone with complex procedures to a minimum of economic effort.

• Korean Journal of Environmental Agriculture
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• v.32 no.1
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• pp.48-54
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• 2013

BACKGROUND: Sorghum (Sorghum bicolor (L.) Moench) ranks as the 6th most planted crop in the world behind wheat, rice, maize, soybean, and barley. The objective of this study was to identify bio-marker among sorghum cultivars using proteomics approach such as two-dimensional polyacrylamide gel electrophoresis (2-DE) coupled with mass spectrometry (MS). METHODS AND RESULTS: Proteins were extracted from sorghum seed, and separated by 2-DE. Total 652 spots were detected from 4 different sorghum seed after staining of 2-DE with colloidal Coomassie brilliant blue (CBB). Among them, 8 spots were differentially expressed and were identified using MALDI-TOF/TOF mass spectrometry. They were involved in RNA metabolism (spot1, spot 4), heat shock proteins (HSPs, spot 2), storage proteins (spot 3, spot 5, and spot 6), and redox related proteins (spot 8). Eight of these proteins were highly up-regulated in Whinchalsusu (WCS). The HSPs, Cupin family protein, and Globulin were specifically accumulated in WCS. The DEAD-box helicase was expressed in 3 cultivars except for WCS. Ribonuclease T2 and aldo-keto reductase were only expressed in 3 cultivars except for Daepung-susu (DPS). CONCLUSION(S): Functions of identified proteins were mainly involved in RNA metabolism, heat shock protein (HSP), and redox related protein. Thus, they may provide new insight into a better understanding of the charactreization between the cultivars of sorghum.

• Journal of Internet Computing and Services
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• v.12 no.4
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• pp.157-172
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• 2011

The latest active studies on distributed OLAP to adopt a distributed environment are mainly focused on DHT P2P OLAP and Grid OLAP. However, these approaches have its weak points, the P2P OLAP has limitations to multidimensional range queries in the cloud computing environment due to the nature of structured P2P. On the other hand, the Grid OLAP has no regard for adjacency and time series. It focused on its own sub set lookup algorithm. To overcome the above limits, this paper proposes an efficient central managed P2P approach for a cloud computing environment. When a multi-level hybrid P2P method is combined with an index load distribution scheme, the performance of a multi-dimensional range query is enhanced. The proposed scheme makes the OLAP query results of a user to be able to reused by other users' volatile cube search. For this purpose, this paper examines the combination of an aggregation cube hierarchy tree, a quad-tree, and an interval-tree as an efficient index structure. As a result, the proposed cloud P2P OLAP scheme can manage the adjacency and time series factor of an OLAP query. The performance of the proposed scheme is analyzed by a series of experiments to identify its various characteristics.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.31 no.4
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• pp.326-339
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• 1995

In this paper, the validity of the numerical model of fishes' behavior presented in our earlier paper was examined by the whiteness test on the residual of numerical model and by the comparison between experiment and simulation on several indexes represented by fishes' swimming characteristics. The validity of the numerical model was proved statistically by means of the whiteness test of the residual. The similarity was confirmed by comparison between experiment and simulation for the swimming trajectory of fishes, the mean distance of individual from wall, the mean swimming speed and the mean distance between the nearest individuals. These results suggest that the behavior of fishes according to the flow speed in three-dimensional space can be estimated partially by the numerical model presented in our earlier paper. However, a long-term approach to improve the modeling technique on the behavior of fishes may be needed before applying the numerical model presented in our earlier paper to real fishing ground.

• Radiation Oncology Journal
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• v.36 no.1
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• pp.17-24
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• 2018

Purpose: This study aimed to assess complications and outcomes of a new approach, that is, combining short course radiotherapy (SRT), concurrent and consolidative chemotherapies, and delayed surgery. Materials and Methods: In this single arm phase II prospective clinical trial, patients with T3-4 or N+ M0 rectal adenocarcinoma were enrolled. Patients who received induction chemotherapy or previous pelvic radiotherapy were excluded. Study protocol consisted of three-dimensional conformal SRT (25 Gy in 5 fractions in 1 week) with concurrent and consolidation chemotherapies including capecitabine and oxaliplatin. Total mesorectal excision was done at least 8 weeks after the last fraction of radiotherapy. Primary outcome was complete pathologic response and secondary outcomes were treatment related complications. Results: Thirty-three patients completed the planned preoperative chemoradiation and 26 of them underwent surgery (24 low anterior resection and 2 abdominoperineal resection). Acute proctitis grades 2 and 3 were seen in 11 (33.3%) and 7 (21.2%) patients, respectively. There were no grades 3 and 4 subacute hematologic and non-hematologic (genitourinary and peripheral neuropathy) toxicities and perioperative morbidities such as anastomose leakage. Grade 2 or higher late toxicities were observed among 29.6% of the patients. Complete pathologic response was achieved in 8 (30.8%) patients who underwent surgery. The 3-year overall survival and local control rates were 65% and 94%, respectively. Conclusion: This study showed that SRT combined with concurrent and consolidation chemotherapies followed by delayed surgery is not only feasible and tolerable without significant toxicity but also, associated with promising complete pathologic response rates.

• Journal of Internet Computing and Services
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• v.9 no.5
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• pp.117-130
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• 2008

This paper proposes a stable and robust method to distinct the color similarity for clothes using the LBG algorithm under various light sources, Since the conventional methods, such as the histogram intersection and the accumulated histogram, are profoundly sensitive to the changing of light environments, the distinction of color similarity for the same cloth can be different due to the complicated light sources. To reduce the effects of the light sources, the properties of hue and saturation which consistently sustain the characteristic of the color under the various changes of light sources are analyzed to define the characteristic of the color distribution. In a two-dimensional space determined by the properties of hue and saturation, the LBG algorithm, a non-parametric clustering approach, is applied to examine the color distribution of images for each clothes. The color similarity of images is defined by the average of Euclidean distance between the mapping clusters which are calculated from the result of clustering of both images. To prove the stability of the proposed method, the results of the color similarity between our method and the traditional histogram analysis based methods are compared using a dozen of cloth examples that obtained under different light environments. Our method successively provides the classification between the same cloth image pair and the different cloth image pair and this classification of color similarity for clothe images obtains the 91.6% of success rate.

• Tunnel and Underground Space
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• v.15 no.1 s.54
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• pp.28-38
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• 2005

According to the recent development of measurement system utilizing one or a set of boreholes, visualization of the explored underground became to be a major issue. It induced even the introduction of monitoring apparatuses on the borehole wall with multi-function tool, but the usage of these was often limited by where is unfavorable rock condition and a few of engineers can approach. And so, a portable type of borehole camera with only the essential function has been investigated and a few of commercial models about this is recently being applied into the field condition. This paper was based on the monitoring results obtained using a commercial model by Dr. Nakagawa. Discontinuities in rock mass were the topic for the visualization, and it was studied how can visualize their three dimensional distribution and what a numerical formulation is needed and how to understand the visualization result. The numerical formulation was based on the geometric correlation between the dip direction / dip of discontinuous plane and the trend / plunge of borehole, a set of the equation of a plane was induced. As field application of this into two places, it is found that the above visualization methodology will be especially an useful geotechlical tool for analyzing the local distribution of discontinuities.