• Asian-Australasian Journal of Animal Sciences
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• v.7 no.4
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• pp.487-491
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• 1994

The experiment was carried out to study the digestibility of nutrients in the neutralized urea-treated rice straw when it was fed singly or in combination with concentrates. A total of 8 crossbred Holstein steers were randomly allocated in a $4{\times}4$ Latin square design consisted of 4 treatments, in which the neutralized straw/concentrates ratio on DM basis varied as 100/0, 90/10, 80/20 and 70/30. The results indicated that the digestibility of DM, ether extract and NFE, and TDN and DE of the diets tended to increase with an increase in the level of concentrates. Regression analysis showed that the values of intercepts should be used for estimating DM digestibility, TDN and DE of neutralized straw, when it was fed with concentrates. Digestibilities of crude fiber, NDF and ADF tended to be higher when it was fed singly than when fed with concentrates. Digestibilities of organic matter and CP were not so much changed with the increasing level of concentrates. Although the animals singly fed the neutralized straw showed positive body weight gain and N-balance, it should be necessary to supplement the concentrates to get more body weight gain and N-balance in the crossbred Holstein steers.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.1
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• pp.51-56
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• 2017

Objective: The objective of this study was to evaluate the intake and nutrient digestibility, nitrogen balance and ruminal ammonia nitrogen in lambs of diets containing different levels of residual frying oil. Methods: Levels of 0, 20, 40, 60, and 80 g/kg dry matter (DM) base of residual frying oil in the diets of lambs were evaluated. Five castrated lambs with initial body weights of $36.8{\pm}3.3kg$, distributed in a Latin square ($5{\times}5$) design, were used. Results: There was a decreasing linear effect on the intake of DM, organic matter (OM), crude protein (CP), neutral detergent fiber (NDF), total carbohydrates (TCH), and nonfibrous carbohydrates (NFC). There was an increased linear intake of ether extract (EE). The apparent digestibility of DM, OM, CP, NDF, TCH, and NFC, as well as urine nitrogen excretion, nitrogen balance and ruminal parameters, were not influenced by different levels of residual frying oil in the diet. EE digestibility presented a crescent linear effect. Conclusion: It can be concluded that the addition of residual frying oil to the diets of sheep can affect nutrient intake without affecting the digestibility of most nutrients (with the exception of EE), nitrogen balance and ruminal ammonia nitrogen concentration.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.10
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• pp.1483-1489
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• 2016

Most of amino acid (AA) digestibility values for feed ingredients are obtained using pigs cannulated in the distal ileum. The ileal-cannulated pig model uses pigs older than six weeks due to difficulties related to implanting the T-cannula in distal ileum of younger pigs and complications during the post-surgical recovery. However, to properly formulate the diet of weaned pigs, the nutritive value of feed ingredients should be determined with younger pigs. Thus, 25 weaned pigs were used to determine the apparent total tract digestibility (ATTD) of nutrients, energy, and apparent ileal digestibility (AID) and standardized ileal digestibility (SID) ileal AA digestibility of broken rice (BR), with or without multicarbohydrase (MC) and phytase (Phy) supplementation. Piglets were weaned at 23 d of age and individually housed in digestibility cages until 45 d of age. The trial consisted of 7 d of adaptation to the experimental diets and 3 d of excreta (feces and urine) collection. Ileal digesta was collected at slaughter (about 6 weeks of age). A completely randomized experimental design was used to determine the effects of MC and Phy. Reference diets (RD, 5% casein) was replaced by 30% of BR with or without MC, Phy, or MC+Phy. The RD was used to quantify endogenous AA losses. BR with Phy supplied had increased the ATTD of dry matter (p<0.05) and SID of histidine (p = 0.05), arginine, leucine, lysine, valine, alanine, and proline (p<0.05). BR with MC had been increased digestible energy and protein and SID for histidine (p<0.05). There was no interaction between Phy and MC on the BR nutrient digestibilities. Standardized amino acid digestibilities of BR, without enzymes, were lower than those values reported in the literature. The MC and Phy improved the digestibility of some nutrients and energy of BR in post-weaned piglet diets.

• Asian-Australasian Journal of Animal Sciences
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• v.5 no.3
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• pp.549-558
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• 1992

The study on the potential use of pigeon pea (PP) as a ruminant feed was carried out with sheep in 3 experiments. Digestibility of dry pigeon pea leaves (PPL) and pigeon pea seeds (PPS) determined by differential and regression methods respectively, with rice straw (RS) as a basal diet, revealed that PPS contained higher nutritive value and palatability than PPL. On dry matter (DM) basis, PPL and PPS contained 19.8 and 20.0% CP, 7.3 and 2.3% EE, 6.0 and 4.4% ash, 61.1 and 51.7% NDF, and 29.4 and 17.5% ADF, respectively. The trypsin inhibitor activity in the seed was 3 times of that in the leaves (19.5 vs 7.0 mg TIA/g DM). The digestibility of PPL and PPS were 50.2 and 72.2% in DM, 52.7 and 73.3% in OM, 51.0 and 65.1% in CP respectively. DM intake as well as the digestibility of most nutrients increased with the increasing level of PPS. Digestible energy (DE), Total digestible nutrient (TDN) and N-balance of sheep fed solely PPS, estimated by regression method, was 3.2 kcal/g, 71.1% and 6.3 g/d respectively. Pigeon pea seeds can be well used to substitute soybean meal in concentrate rations for ruminants or directly supplemented to low quality roughages.

• Journal of Animal Science and Technology
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• v.58 no.2
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• pp.9.1-9.5
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• 2016

Background: Literature is lacking on the effects of feeding by-product feed (BF)-based silage on rumen fermentation parameters, nutrient digestion and nitrogen (N) retention in sheep. Therefore, this study was conducted to determine the effect of replacing rye straw with BF-based silage as a roughage source on ruminal parameters, total-tract apparent nutrient digestibility, and N balance in sheep. Methods: The by-product feed silage was composed of spent mushroom substrate (SMS) (45 %), recycled poultry bedding (RPB) (21 %), rye straw (11 %), rice bran (10.8 %), corn taffy residue (10 %), protected fat (1.0 %), bentonite (0.6 %), and mixed microbial additive (0.6 %). Six sheep were assigned randomly to either the control (concentrate mix + rye straw) or a treatment diet (concentrate mix + BF-based silage). Results: Compared with the control diet, feeding a BF-based silage diet resulted in similar ruminal characteristics (pH, acetate, propionate, and butyrate concentrations, and acetate: propionate ratio), higher (p < 0.05) ruminal NH3-N, higher (p < 0.05) ether extract digestibility, similar crude protein digestibility, lower (p < 0.05) dry matter, fiber, and crude ash digestibilities, and higher (p < 0.05) N retention (g/d) Conclusion: The BF-based silage showed similar energy value, higher protein metabolism and utilization, and lower fiber digestion in sheep compared to the control diet containing rye straw.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.2
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• pp.215-222
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• 2015

The objective of this research was to evaluate the growth performance, the apparent ileal digestibility of nitrogen and energy, the retention of nutrients and the apparent metabolizable energy corrected to zero nitrogen retention (AMEn) in broiler chickens supplemented with increasing doses of a worm leachate (WL) as a source of humic substances (HS) in the drinking water. In Exp. 1, 140 male broilers were penned individually and assigned to four WL levels (0%, 10%, 20%, and 30%) mixed in the drinking water from 21 to 49 days of age. Water was offered in plastic bottles tied to the cage. In Exp. 2, 600 male broilers from 21 to 49 days of age housed in floor pens were assigned to three levels of WL (0%, 10%, and 20%) mixed in the drinking water. The WL was mixed with tap water in plastic containers connected by plastic tubing to bell drinkers. The results of both experiments were subjected to analysis of variance and polynomial contrasts. In Exp. 1, the daily water consumption was similar among treatments but the consumption of humic, fulvic, and total humic acids increased linearly (p<0.01) as the WL increased in the drinking water. The feed conversion (p<0.01) and the ileal digestibility of energy, the excretion of dry matter and energy, the retention of dry matter, ash and nitrogen and the AMEn showed quadratic responses (p<0.05) relative to the WL levels in drinking water. In Exp. 2, the increasing level of WL in the drinking water had quadratic effects on the final body weight, daily weight gain and feed conversion ratio (p<0.05). The addition of WL as a source of HS in the drinking water had beneficial effects on the growth performance, ileal digestibility of energy, the retention of nutrients as well on the AMEn in broiler chickens; the best results were observed when the WL was mixed at levels of 20% to 30% in the drinking water.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.3
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• pp.317-322
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• 2000

In situ and in vivo digestion trials were conducted to determine the degradation of dry matter (DM), crude protein (CP) and effective protein degtadability (EPD), and digestibility of nutrients of Hazelnut kernel oil meal (HKOM), and effects of HKOM on nitrogen (N) balance. In the in situ study, nylon bag were suspended in the rumen of 3 Karayaka rams to estimate protected protein. Protein sources were analyzed for pepsin soluble protein (PSP) using a Pepsin Digestion Method. In the digestion trials, 4 Karayaka rams (36 mo.) were used in a $4{\times}4$ Latin square to evaluate the digestibility of nutrients and N retention to measure effects of diets containing HKOM, soybean meal (SBM) corn gluten meal (CGM) and urea (U). The degradability of DM and CP, and PSP content of HKOM were lower (p>0.05) than that of SBM, but higher (p<0.001) than that of CGM. EPD of HKOM was higher (p<0.01) than that of SBM or CGM. The apparent digestion coefficients of organic matter and CP for HKOM were lower than for SBM, but higher than for CGM. N retention of HKOM was higher than that of SBM and lower than that of CGM (p>0.05). In conclusion, these data may indicate that the HKOM is a high digestible feed source with a value between SBM and CGM.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.7
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• pp.967-973
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• 2000

A metabolism trial was conducted with 28 Najdi rams allocated into seven dietary groups to evaluate the effect of dietary inclusion of Salicornia bigelovii Torr biomass on nutrient digestibility, rumina I fluid metabolites and nitrogen and mineral balances. Either the stems (ST) or spikes (SP) of this seawater-irrigated halophyte were incorporated into complete diets at rates of 0, 10, 20 and 30% levels, replacing equal amounts of rhodesgrass hay in a ground mixed control diet. Digestibility of DM, OM, EE, NFE and fecal and urinary nitrogen were not affected by increased level of ST in the diet. As level of ST increased from 0 to 20% in the diets, CP digestibility and nitrogen retention approached their maximum (p<0.01), whereas CF digestibility reached its minimum (p<0.01). On the other hand, except for EE, digestion of all nutrients and nitrogen retention were linearly depressed (p<0.01) as SP increased in the diets from 10 to 30% level. Concentration of ammonia-N, total VFA and pH values in the rumen fluid were lower (p<0.01) with the ST- or SP-fed diets than with the control diet. Increasing level of ST or SP in the diet was associated with an increase (p<0.01) in the proportion of acetate and a decline (p<0.01) in molar percentage of propionate in the ruminal fluid. Sodium absorption increased (p<0.01) with increased ST and SP in the diets up to the 10 and 20% level, respectively, followed by constant absorption values up to the 30% level. When the level of ST in the diet gradually increased to 30%, a concomitant increased (p<0:01) in Ca and P absorption were obvious; whereas, increased level of SP in the diets from 0 to 30% resulted in noticeable (p<0.01) depression in Ca and P apparent absorption.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.2
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• pp.197-202
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• 1999

Three barrows weighing 45.0 kg, fitted with simple T-cannulas in both the duodenum and terminal ileum, were assigned to diets in a $3{\times}3$ Latin Square design experiment to determine the effect of two calcium levels (0.8% vs 0.4%) on phytase activity and nutrient balance in growing pigs. The control diet contained 0.8% calcium, with no added inorganic phosphorus (0.45% total phosphorus) and no added phytase. The two additional experimental diets contained microbial phytase (750 phytase units/kg) and supplied either 0.8% or 0.4% calcium. With added microbial phytase, ileal and total tract digestibility of rotal phosphorus were improved by 20.9 and 13.8 percentage units, respectively (p=0.01). The apparent duodenal and ileal digestibility of phytate phosphorus were increased by 51.8 and 49.7 percentage units (p=0.01). Lowering dietary calcium in the presence of microbial phytase increased the digestibility of phytate phosphorus by an additional 10.9 (p=0.001) and 5.7 percentage units for duodenal and ileal digestibility, respectively. Supplementation with microbial phytase significantly reduced fecal excretion of nitrogen and phosphorus and increased the percentage of these nutrients retained by the pig. Lowering dietary calcium further increased the percentage of dietary phosphorus retained. Overall, reducing dietary calcium appeared to increase the effectiveness of added microbial phytase in degrading phytate phosphorus. As a result, care should be taken to avoid high levels of dietary calcium when supplementing swine diets with microbial phytase.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.10
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• pp.1422-1428
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• 2006

In order to investigate the effect of different levels of niacin supplementation on growth, nutrient utilization, their balance and blood biochemical profile, 15 male buffalo calves (9-10 months of age, $88.4{\pm}4.37kg$ average body weight) were divided into 3 equal groups each of 5 calves, following a completely randomized design, and fed individually for 120 days with wheat straw and concentrate mixture to meet their nutrient requirements. In addition calves were supplemented with 0 ppm (control, group I), 100 ppm (group II) and 200 ppm (group III) niacin. After 90 days of experimental feeding a metabolism trial was conducted to estimate the digestibility of nutrients and their balance. Fortnightly body weights were recorded to assess their growth rate and blood was collected from the jugular vein at day 0 and subsequently at 30-day intervals from all the experimental buffalo calves to study blood biochemical parameters. Results showed that intake and digestibility of dry matter, organic matter, crude protein, ether extract, total carbohydrates, neutral detergent fibre, acid detergent fibre, cellulose and hemicelluloses were statistically similar in the 3 groups. Buffalo calves in all three groups were in positive nitrogen, calcium and phosphorus balance, without showing any significant effect of the treatments. Dry matter, crude protein, digestible crude protein and total digestible nutrient intake ($g/d/kgW^{0.75}$) were similar in the control and niacin supplemented groups. Digestible crude protein (%) and total digestible nutrients (%) in the ration of the 3 groups were 8.07, 7.99, 7.92 and 56.70, 56.63, 56.74, respectively, and were comparable among the groups. The average daily gain (g) in-group II (567.50) was not significantly (p>0.05) higher than group I (500.0) and group III (510.0). Blood biochemical constituents (glucose, total protein, albumin, globulin, urea-N, insulin) showed no significant effect of niacin supplementation. However, serum cholesterol (mg/100 ml) was significantly (p<0.01) lower in the 200 ppm niacin-supplemented group than in the control and 100 ppm niacin-supplemented groups. It can be concluded that supplementation of niacin at 100 and 200 ppm in the diet of buffalo calves had no significant beneficial effect on their growth and nutrient utilization.