• The Plant Pathology Journal
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• 제32권4호
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• pp.347-356
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• 2016

Plants protect themselves from pathogen attacks via several mechanisms, including hypersensitive cell death. Recognition of pathogen attack by the plant resistance gene triggers expression of carboxylesterase genes associated with hypersensitive response. We identified six transcripts of carboxylesterase genes, Vitis flexuosa carboxylesterase 5585 (VfCXE5585), Vf-CXE12827, VfCXE13132, VfCXE17159, VfCXE18231, and VfCXE47674, which showed different expression patterns upon transcriptome analysis of V. flexuosa inoculated with Elsinoe ampelina. The lengths of genes ranged from 1,098 to 1,629 bp, and their encoded proteins consisted of 309 to 335 amino acids. The predicted amino acid sequences showed hydrolase like domains in all six transcripts and contained two conserved motifs, GXSXG of serine hydrolase characteristics and HGGGF related to the carboxylesterase family. The deduced amino acid sequence also contained a potential catalytic triad consisted of serine, aspartic acid and histidine. Of the six transcripts, Vf-CXE12827 showed upregulated expression against E. ampelina at all time points. Three genes (VfCXE5585, VfCXE12827, and VfCXE13132) showed upregulation, while others (VfCXE17159, VfCXE18231, and VfCXE47674) were down regulated in grapevines infected with Botrytis cinerea. All transcripts showed upregulated expression against Rhizobium vitis at early and later time points except VfCXE12827, and were downregulated for up to 48 hours post inoculation (hpi) after upregulation at 1 hpi in response to R. vitis infection. All tested genes showed high and differential expression in response to pathogens, indicating that they all may play a role in defense pathways during pathogen infection in grapevines.

• 한국축산식품학회지
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• 제36권6호
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• pp.791-798
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• 2016

Free radicals may attack cells or tissue, leading to chronic diseases, and antioxidant consumption is potentially useful for removing free radicals. Egg proteins may be used as potential sources of antioxidant considering their ability of scavenging free radicals to apply for food or cosmetics industry. In this study, we obtained a natural antioxidant protein from fertilized eggs, which was a dietary supplement in some Asian countries. Meanwhile, antioxidant activities of these proteins were evaluated using different oxidation systems. With increasing incubation time, the antioxidant activity of these proteins increased during 15 d of incubation. The samples on day 15 were performed for isolation of antioxidant protein. The protein, named P4-1 (MW, 45 kDa), was isolated and purified by consecutive chromatographic methods. P4-1 contained 17 amino acids, which was determined by liquid chromatography-mass spectrometry and Amino Acid Analyzer. Moreover, the amino acid sequence was highly similar to that of ovalbumin. Differential scanning calorimetry showed that the denaturation temperature of P4-1 was $57.16^{\circ}C$. Furthermore, P4-1 suggested high oxygen radical-absorbance activity in ${\cdot}OH$ assays, and its antioxidant activity was stable at $30-50^{\circ}C$ in acidic and neutral pH. Thus, these results revealed that P4-1 may be a potential resource as a natural antioxidant.

• 정보보호학회논문지
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• 제12권2호
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• pp.65-76
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• 2002

신상욱 등은 PKC'98에서 기존 RIPEMD-160, HAVAL, SHA-1와 같은 해쉬 함수의 장점을 이용하여 160비트의 출력 길이를 갖는 새로운 해쉬 함수를 제안하였다.$^{[1]}$ 최근 FSE 2002에서 한 대완 등은 PKC'98에 제안된 해쉬 함수의 부울 함수가 당초 설계자의 의도와는 달리 일부 부울 함수가 SAC(Strict Avalanche Criterian)을 만족하지 않음을 지적하고, 설계자의 의도에 맞게 모든 부울 함수가 SAC의 성질을 만족한다는 가정 하에, $2^{-30}$의 확률로 충돌 쌍을 찾는 공격방법을 제안하였다.$^{[2]}$ 본 논문에서는 위의 방법을 개선하여, PKC'98에서 제안된 해쉬 함수의 origin version의 전체라운드에 대해 2^{-37.13}$의 확률로 충돌 쌍을 찾을 수 있음을 보인다. 그리고 PKC'98에 제안된 해쉬 함수의 문제점이 메시지에 의존한 쉬프트 값의 사용에 있음을 지적한다.

• 스마트미디어저널
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• 제12권11호
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• pp.36-47
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• 2023

주요 산업현장에서 설비를 제어하는 산업제어시스템(ICS, Industrial Control System)이 네트워크로 다른 시스템과 연결되는 사례가 증가하고 있다. 또한, 이러한 통합과 함께 한 번의 외부 침입이 전체 시스템 마비로 이루어질 수 있는 지능화된 공격의 발달로, 산업제어시스템에 대한 보안에 대한 위험성과 파급력이 증가하고 있어, 사이버 공격에 대한 보호 및 탐지 방안의 연구가 활발하게 진행되고 있으며, 비지도학습 형태의 딥러닝 모델이 많은 성과를 보여 딥러닝을 기반으로 한 이상(Anomaly) 탐지 기술이 많이 도입되고 있다. 어어, 본 연구에서는 딥러닝 모델에 전처리 방법론을 적용하여 시계열 데이터의 이상 탐지성능을 향상시키는 것에 중점을 두어, 그 결과 웨이블릿 변환(WT, Wavelet Transform) 기반 노이즈 제거 방법론이 딥러닝 기반 이상 탐지의 전처리 방법론으로 효과적임을 알 수 있었으며, 특히 센서에 대한 군집화(Clustering)를 통해 센서의 특성을 반영하여 Dual-Tree Complex 웨이블릿 변환을 차등적으로 적용하였을 때 사이버 공격의 탐지성능을 높이는 것에 가장 효과적임을 확인하였다.

• 수면정신생리
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• 제7권2호
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• pp.115-119
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• 2000

목 적 : 십년 이상 각성장애를 보인 20세 남자환자를 분석하였다. 환자는 두통, 만성피로, 경한 주간졸리움증을 호소하였으나 조절할 수 없는 수면발작, 탈력발작, 입면시 환각이나 수면마비의 병력은 없었다. 방 법 : 야간 수면다원검사 (PSG), 반복적 수면잠복기 검사 (MSLT) 및 조직적합성 유전자검사 (HLA-typing)를 시행 하였다. 결 과 : PSG상 수면잠복기가 짧고 (4분), 렘수면잠복기도 감소하였고 (2.5분), 각성지표 (arousal index)가 시간당 15.7로 약간 증가되었으며, 수면 중 주기적 사지운동지표 (PLMS index)가 시간당 8.1로 관찰되었으나 운동과 연관된 각성지표 (movement arousal index)는 시간당 2.1로 높지 않았다. 잠효율은 (sleep efficiency)는 97.5%로 정상이었다. MSLT상 수면잠복기는 15분 21초로 정상이었으나 sleep-onset REM (SOREM)은 5회의 낮잠 시도 중 4회에서 관찰되었다. HLA-typing에서 DQ6-양성이었는데, 이는 기면증 환자에서 대개 관찰되는 유전자 위치인 DQB1*0602, DQA1*0102와는 다른 DQB1*0601 부위에 상응하였다. 결 론 : 일차성 각성장애의 원인이 되는 여러 질환 특히 일주기리듬장애나 기면증, 원발성 다면증과의 감별진단이 필요하며, 수면검사와 유전자검사 상 기면증의 새로운 변종일 가능성을 배제할 수 없다.

• The Plant Pathology Journal
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• 제30권4호
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• pp.343-354
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• 2014

Rice blast disease caused by Magnaporthe oryzae is one of the most serious diseases of cultivated rice (Oryza sativa L.) in most rice-growing regions of the world. In order to investigate early response genes in rice, we utilized the transcriptome analysis approach using a 300 K tilling microarray to rice leaves infected with compatible and incompatible M. oryzae strains. Prior to the microarray experiment, total RNA was validated by measuring the differential expression of rice defense-related marker genes (chitinase 2, barwin, PBZ1, and PR-10) by RT-PCR, and phytoalexins (sakuranetin and momilactone A) with HPLC. Microarray analysis revealed that 231 genes were up-regulated (>2 fold change, p < 0.05) in the incompatible interaction compared to the compatible one. Highly expressed genes were functionally characterized into metabolic processes and oxidation-reduction categories. The oxidative stress response was induced in both early and later infection stages. Biotic stress overview from MapMan analysis revealed that the phytohormone ethylene as well as signaling molecules jasmonic acid and salicylic acid is important for defense gene regulation. WRKY and Myb transcription factors were also involved in signal transduction processes. Additionally, receptor-like kinases were more likely associated with the defense response, and their expression patterns were validated by RT-PCR. Our results suggest that candidate genes, including receptor-like protein kinases, may play a key role in disease resistance against M. oryzae attack.

• Journal of Chest Surgery
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• 제8권2호
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• pp.109-118
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• 1975

This is a report on four cases of the lobar emphysema due to proximal bronchial obstruction in the Department of Thoracic Surgery, Hanyang University Hospital, during the period of three and half years from 1972 to 1975. First case, a two years old male child was referred to our Department with the lobar emphysema of the lower lobe of the right lung with pneumonia. This emphysema was developed after aspiration of a piece of peanut. Bronchoscopy revealed that the bronchus of the right lower lobe was obstructed with the foreign body, however removal of the peanut through bronchoscope was not attempted because of corruption and softening of the peanut. The removal of the peanut by bronchotomy was performed after subsiding of acute phase of pulmonary infection. Postoperative course was uneventful and the emphysema was disappeared. Second case, a twenty months old female baby was referred to our Department with lobar emphysema of the lower lobe of the left lung. The emphysema was suddenly developed with coughing and dyspneic symptoms and the diagnosis was made roentgenologically. She gave a history of reccurrent infections of the respiratory tract after birth. Bronchoscopy showed an obstruction of the left main bronchus with the growing of fibrinous tissue on the bronchial mucosa. The protruded tissue in the left main bronchus taken out about O.8ml with biopsy forceps for histological examination. After this procedure, the emphysema of the left lung was disappeared. Histological finding was reported to be a chronic inflammatory granulation tissue. Third case, a two and half years old male child was referred to our Department with roentgenological lobar emphysema. Two weeks prior to admission he had an episode of sudden onset of coughing attack with dyspnea. Bronchoscopy revealed that the bronchus of the left lower lobe was obstructed with a mass which was strongly suspected of a neoplastic tissue. At operation, there was found a perforation of enlarged tuberculous lymph node in the bronchus of the left lower lobe and protrusion of granulation tissue into the bronchus. Ruptured orifice on themembranous wall of the left lower lobe bronchus was closed with interrupted suture after the" removal of a perforated tuberculous lymph node. Postoperative course was uneventful and antituberculous chemotherapy was given. Fourth case, a 47 years old man was admitted to our Department with the complaint of severe dyspnea of few months duration. Twenty years ago, he had a history of lung tuberculosis and was treated for many years. X-ray examination including tomography and bronchography revealed that the upper lobe of the right lung was destroyed with cavities, the lower lobe was completely shrunk, and the right middle lobe was strongly overdistended with narrowing bronchial trees. Differential bronchospirometry and lung scanning confirmed that the respiratory function of the affected lung was impaired almost totally. The value of the right lung was calculated on 6% of oxygen uptake, 1% of Minute volume, and 32% of vital capacity. The right pneumonectomy was performed under the careful consideration of anesthetic and surgical procedures. Postoperative course was uneventful and the respiratory function was improved nearly to the normal level.evel.

• 정보보호학회논문지
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• 제18권5호
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• pp.93-101
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• 2008

스마트카드, USB token과 같은 저 전력 정보보호장치의 가장 큰 위협요소인 부채널 공격은 장치 내부에 구현된 암호 알고리즘의 이론적인 안전도와는 무관하게 적용될 수 있다. 특히, 부채널 공격들 중에서 차분 전력분석 공격은 적용이 쉽고 근본적인 방어가 어려워서 매우 위협적인 공격이지만 공격을 적용하기 위해서는 측정된 모든 신호가 시간축 상에서 매우 잘 정렬된 신호라는 전제조건이 필요하기 때문에, 트리거 지터링, 잡음, 차분 전력 분석 공격 방어책 등 여러 요인들에 의해 시간축 상에 정렬되지 않은 측정된 신호를 정렬하기 위한 여러 가지 방법들이 제안되어 왔다. 기존의 신호 정렬 방법들은 측정된 신호의 시간축 상의 위치만을 정렬하는 방법들이어서, 랜덤 클럭을 이용하여 알고리즘의 수행 시간(시간축 상의 신호 크기)을 변화시키는 차분 전력 분석 대응 방법에는 적용이 되지 않는다. 본 논문에서는 측정된 소비 전력 신호를 보간(interpolation)과 추출(decimation) 과정을 통해서 시간축 상에서 위치뿐만 아니라 크기도 동시에 정렬시키는 향상된 신호 정렬 방법을 제안하였다. 또한 랜덤 클럭 방식의 차분 전력 분석 공격 방어대책이 구현된 스마트카드 칩에 개선된 신호 정렬 방법을 적용하여 차분 전력 분석 공격이 효과적으로 적용됨을 실험적으로 확인하였다.

• IMMUNE NETWORK
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• 제24권2호
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• pp.17.1-17.16
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• 2024

We have reported that anterior cruciate ligament (ACL) injury leads to the differential dysregulation of the complement system in the synovium as compared to meniscus tear (MT) and proposed this as a mechanism for a greater post-injury prevalence of post traumatic osteoarthritis (PTOA). To explore additional roles of complement proteins and regulators, we determined the presence of decay-accelerating factor (DAF), C5b, and membrane attack complexes (MACs, C5b-9) in discarded surgical synovial tissue (DSST) collected during arthroscopic ACL reconstructive surgery, MT-related meniscectomy, osteoarthritis (OA)-related knee replacement surgery and normal controls. Multiplexed immunohistochemistry was used to detect and quantify complement proteins. To explore the involvement of body mass index (BMI), after these 2 injuries, we examined correlations among DAF, C5b, MAC and BMI. Using these approaches, we found that synovial cells after ACL injury expressed a significantly lower level of DAF as compared to MT (p<0.049). In contrast, C5b staining synovial cells were significantly higher after ACL injury (p<0.0009) and in OA DSST (p<0.039) compared to MT. Interestingly, there were significantly positive correlations between DAF & C5b (r=0.75, p<0.018) and DAF & C5b (r=0.64 p<0.022) after ACL injury and MT, respectively. The data support that DAF, which should normally dampen C5b deposition due to its regulatory activities on C3/C5 convertases, does not appear to exhibit that function in inflamed synovia following either ACL injury or MT. Ineffective DAF regulation may be an additional mechanism by which relatively uncontrolled complement activation damages tissue in these injury states.