• 수산해양기술연구
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• 제43권2호
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• pp.140-148
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• 2007

This paper describes on the real-time monitoring of dredging information for grab bucket dredger equipped with winch control sensors and differential global positioning system(DGPS) using electronic chart display and information system(ECDIS). The experiment was carried out at Gwangyang Hang and Gangwon-do Oho-ri on board M/V Kunwoong G-16. ECDIS system monitors consecutively the dredging's position, heading and shooting point of grab bucket in real-time through 3 DGPS attached to the top bridge of the dredger and crane frame. Dredging depth was measured by 2 up/down counter fitted with crane winch of the dredger. The depth and area of dredging in each shooting point of grab bucket are displayed in color band. The efficiency of its operation can be ensured by adjusting the tidal data in real-time and displaying the depth of dredging on the ECDIS monitor. The reliance for verification of dredging operation as well as supervision of dredging process was greatly enhanced by providing three-dimensional map with variation of dredging depth in real time. The results will contribute to establishing the system which can monitor and record the whole dredging operations in real-time as well as verify the result of dredging quantitatively.

• BMB Reports
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• 제33권5호
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• pp.396-401
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• 2000

A differential display method is used to identify novel genes whose expression is affected by treatment with ferric ammonium citrate (FAC) or desferrioxamine (DFO), an iron chelating agent in the human hepatoblastoma cell line (HepG2). These chemicals are known to deplete or increase the intracellular concentration of iron, respectively. Initially, we isolated seventeen genes whose expressions are down- or up regulated by the treatment of the chemicals, as well as their four differentially expressed genes that are designated as clone-1, -2, -3, and -4. These are further characterized by cDNA sequencing and Northern blot analysis. Through the cDNA sequencing, as well as comparing them to genes published using the NCBI BLAST program, we identified the sequence of the clone-1 that is up-regulated by the treatment of DFO. It is identical to the human insulin-like growth factor binding protein-1 (IGFBP-1). This suggests that the IGFBP-1 gene in the HepG2 cell is up-regulated by an iron depletion condition. Also, the expression of the clone-3 and -4 is up-regulated by FAC treatment and their eDNA sequences are identical to the human ferritin-fight chain and human NADH-dehydrogenase, respectively. However, the sequence of the clone-2 has no significant homology to any other known gene. Therefore, we suggest that changes of the cellular iron level in the HepG2 cell affects the transcription of cellular genes. This includes human IGFBP-1, ferritin-fight chain, and NADH-dehydrogenase. Regulation of these gene expressions may have an important role in cellular functions that are related to cellular iron metabolism.

• Molecules and Cells
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• 제25권1호
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• pp.78-85
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• 2008

In a search for new molecular pathways associated with asthma, we performed an mRNA differential display analysis using total RNA extracted from the tracheal tissues of ovalbumin (OVA)-challenged mice and sham controls. cDNAs corresponding to mRNAs for which expression levels were altered by OVA-challenge were isolate and sequenced. Twenty-eight genes differentially expressed in sham and OVA challenged mice were identified. A GenBank BLAST homology search revealed that they were related to cytoskeleton remodeling, transcription, protein synthesis and modification, energy production, and cell growth and differentiation. Two were selected for further characterization. Up-regulation of both the perinatal skeletal myosin heavy chain (skMHC) and fast skeletal muscle myosin light chain (skMLC) genes was confirmed by RT-PCR of trachea tissue from OVA challenged mice. Overexpression of skMLC protein was observed in the smooth muscle layers of OVA-challenged mice by immunohistochemistry, and the surface areas stained with skMLC antibody increased in the OVA-challenged mice. The overexpression of skMLC in murine asthma may be associated with the changes of bronchial smooth muscle.

• 생물정신의학
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• 제6권1호
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• pp.67-73
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• 1999

The cAMP-dependent protein kinase(PKA) is an intracellular enzyme with serine-threonine kinase activity that plays a key role in cell growth, differentiation, and apoptosis in eukaryotes. In order to understand the PKA signal transduction pathway regulating cell life cycle and identify its role, we focused on the characterization of up-/down-regulated genes by PKA using the differential display polymerase chain reaction. Seven differentially expressed sequence tags(DEST) have been obtained. Among these DESTs, 2 DESTs were homologous to the sequence of genes from BLAST search result. KC1-5 DEST that was up-regulated in A123.7 cells was highly corresponded to mouse apoptosis-related gene(MA-3) or mouse mRNA for topoisomerase inhibitor suppressed(TIS). MA-3 was induced in various types of apoptosis, specially in NGF-deprived apoptotic PC12 cells. TIS was down-regulated in the RVC lymphoma cells incubated with topoisomerase inhibitor that induces DNA strand breakages. PG1-1 DEST that was highly expressed in PC12 cells was corresponded to transposon Tn10 3'-end. Tnansposon Tn10 was up-regulated in differentiated myeloblastic ML-1 cells by 12-O-tetradecanoylphorbol-13-acetate. This study illuminates that MA-3/TIS was down-regulated by PKA activity, and transposon Tn10 was up-regulated by it.

• Journal of Plant Biotechnology
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• 제31권4호
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• pp.273-278
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• 2004

Differential display 방법으로 NaCl에 의하여 발현이 증가되는 cDNA들을 분리하였으며 그중 하나가 식물유래의 outer envelope membrane protein과 높은 유사성을 보였으므로 이를 ShOEP로 명명하였다. ShOEP는 1293 bp 길이에 359개의 아미노산으로 구성된 open reading frame을 포함하고 있으며, 이로부터 추정되는 분자량은 8.9 kDa이었다. ShOEP 단백질은 애기장대의 OEP와는 40.6%, 시금치와는 38%의 유사성을 나타내었다. Northern 분석결과, ShOEP 유전자는 NaCl의 농도가 증가함에 따라 발현량이 급격히 증가하는 것으로 나타났다. 염생식물인 퉁퉁마디의 OEP는 PEG에 의하여 발현이 증가하는 반면 비염생식물인 애기장대의 OEP는 큰 차이를 보이지 않았다. 효모 complementation 실험결과 ShOEP는 NaCl에 특이적이었으며 식물의 염분스트레스 내성 기작에 직접적으로 관여하고 있음을 알 수 있었다.

• 한국융합학회논문지
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• 제8권8호
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• pp.185-190
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• 2017

자연현상에서 발견되는 프랙털 (fractal) 구조는 자기 유사성 (self-similarity)의 반복된 형태로, 생명체의 발생과 기관 형성에서도 자주 관찰된다. 특히, 수많은 종류의 암모나이트에서 관찰되는 봉합선 (suture)의 복잡, 다양한 형태는 프랙털 생성에 대한 생물학적인 이해를 위한 좋은 연구소재이다. 본 연구에서는 희귀하게 초기 발생단계의 봉합선 구조가 매우 잘 보전된 Eogaudryceras sp.를 대상으로 봉합선 형성의 과정을 분석하고자 하였으며, 일반적으로 구하기 힘든 부위인 나선 (spiral) 중심부 1mm 이내의 현미경적인 관찰을 실시하였다. 아직 봉합선의 프랙털 구조 생성에 대한 생물학적인 기전은 아직 불분명한 상태이나, 본 연구를 통하여 암모나이트 발생초기에 프랙털 구조의 복잡성이 단계별로 분명한 차이가 있음을 발견하였다. 이러한 결과는 하나의 생명체 내에서 "Evo-Devo"의 여러 발생단계의 변화를 동시에 보여줄 수 있는 좋은 예시로서, 향후 프랙털 구조의 생물학적인 기전 연구 및 관련되는 다양한 융합학문에서의 접목과 활용이 기대된다.

• 한국발생생물학회지:발생과생식
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• 제9권1호
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• pp.33-41
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• 2005

배반포 단계의 난자에서 차이 나게 발현하는 유전자의 발굴을 통해 초기 동물 발생과 분화에 관한 기전을 알 수 있다. 본 연구에서는 새로운 차별발현 역전사효소중합법, 이름하여 에닐링 콘트롤 프라이머(ACP) 방법에 의해 생쥐 배반포에서 차이 나게 발현하는 유전자를 줄기세포와 비교하여 발굴하였다. 총 100개의 ACP를 사용하여 26개의 유전자 단편을 확인하였고, BLAST 탐색에 의해 유전자 정보 은행(GeneBank/EMBL)에 저장된 유전자와 동일하다는 것을 알았다. 이들 유전자 중에 15개의 유전자를 선별하여 역전사효소중합법에 의해 조사한 결과, 배반포에서 차이 나게 발현함을 재확인하였다. 이러한 결과는 ACP 방법이 특정 발달 단계에 있는 소량의 배아 샘플로부터 전사되는 유전자를 확인하는데 실용적으로 사용될 수 있다는 것을 보여주고 있다.

• 수산해양기술연구
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• 제45권3호
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• pp.165-176
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• 2009

The process of berthing/deberthing operations for entering/leaving vessels in Busan northern harbor was analyzed and evaluated by using an integrated VTS(vessel traffic service) system installed in the ship training center of Pukyong National University, Busan, Korea. The integrated VTS system used in this study was consisted of ARPA radar, ECDIS(electronic chart display and information system), backup(recording) system, CCTV(closed-circuit television) camera system, gyro-compass, differential GPS receiver, anemometer, AIS(automatic identification system), VHF(very high frequency) communication system, etc. The network of these systems was designed to communicate with each other automatically and to exchange the critical information about the course, speed, position and intended routes of other traffic vessels in the navigational channel and Busan northern harbor. To evaluate quantitatively the overall dynamic situation such as maneuvering motions for target vessel and its tugboats while in transit to and from the berth structure inside a harbor, all traffic information in Busan northern harbor was automatically acquired, displayed, evaluated and recorded. The results obtained in this study suggest that the real-time tracking information of traffic vessels acquired by using an integrated VTS system can be used as a useful reference data in evaluating and analyzing exactly the dynamic situation such as the collision between ship and berth structure, in the process of berthing/deberthing operations for entering/leaving vessels in the confined waters and harbor.

• 방송공학회논문지
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• 제19권4호
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• pp.478-490
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• 2014

3-D 디스플레이를 이용하여 실감나는 입체 경험을 시청자에게 제공하는데 필요한 요인을 파악하는 다양한 연구가 여러 분야에서 이루어지고 있다. 본 연구는 3차원 지각에 필요한 정보를 어느 쪽 눈에 제시하느냐에 따라 관찰자의 입체 지각 과정에 차이가 있는지를 알아보고자 하였다. 실험은 관찰자의 우세안과 비우세안에 3-D 자극 패턴의 변화를 배타적으로 제공하고, 이 자극 정보를 통합해서 3-D 입체로 지각하는 시간을 측정하는 과정이 포함되었다. 다양한 조건으로 구성된 실험에서 얻은 결과는 관찰자의 우세안 여부에 상관없이 3-D 정보가 왼쪽 눈에 제시된 경우, 오른쪽 눈에 비해 일관성 있게 지각이 수행된다는 것이다. 본 결과는 3-D 영상물 제작 시 깊이 정보가 왼쪽 눈에 담길 수 있도록 입체 영상 카메라 시점을 조정한다면, 입체 영상물에 담겨있는 깊이를 영상 관람자가 더 잘 지각할 수 있음을 제안하고 있다.

• The Korean Journal of Physiology and Pharmacology
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• 제24권2호
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• pp.165-171
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• 2020

Ischemic and traumatic brain injuries are the major acute central nervous system disorders that need to be adequately diagnosed and treated. To find biomarkers for these acute brain injuries, plasma levels of some specialized pro-resolving mediators (SPMs, i.e., lipoxin A4 [LXA4], resolvin [Rv] E1, RvE2, RvD1 and RvD2), CD59 and interleukin (IL)-6 were measured at 0, 6, 24, 72, and 168 h after global cerebral ischemic (GCI) and traumatic brain injuries (TBI) in rats. Plasma LXA4 levels tended to increase at 24 and 72 h after GCI. Plasma RvE1, RvE2, RvD1, and RvD2 levels showed a biphasic response to GCI; a significant decrease at 6 h with a return to the levels of the sham group at 24 h, and again a decrease at 72 h. Plasma CD59 levels increased at 6 and 24 h post-GCI, and returned to basal levels at 72 h post-GCI. For TBI, plasma LXA4 levels tended to decrease, while RvE1, RvE2, RvD1, and RvD2 showed barely significant changes. Plasma IL-6 levels were significantly increased after GCI and TBI, but with different time courses. These results show that plasma LXA4, RvE1, RvE2, RvD1, RvD2, and CD59 levels display differential responses to GCI and TBI, and need to be evaluated for their usefulness as biomarkers.