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Benthic Polychaetous Community in Northern Kyeonggi Bay in December 1995 (1995년 12월 경기만 북부해역에서와 저서다모류 군집)

  • Shin, H.C.;Koh, C.H.
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.3 no.4
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    • pp.261-270
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    • 1998
  • This study was carried out in order to investigate the distribution and species composition of benthic polychaetous community in northern Kyeonggi Bay in September 1995. Mean density of benthic animals was 557 indiv./$m^2$. Benthic polychaetes were the most dominant faunal group, comprising 60 species with a mean density of 488 indiv./$m^2$. Benthic polychaetes were most abundant in Yumha Channel centering on Incheon Harbor, whereas relatively poor in the intertidal zones fringing Yongjong Island and Songdo area, and in the offshore main channel. The most dominant polychaete was Heteromastus filiformis (47.3% of polychaetous community), followed by Nephtys palybranchia, Tharyx sp., Sternaspis scutata and so on. Kyeonggi Bay could be divided into 4 regions from cluster analysis based on the dominant species composition: offsea/channel region, Incheon Harbor, intertidal zone of Yongjong Island, and intertidal zone of Songdo area. Highest species number and diversity index (H') were recorded at offsea/channel region, whereas highest faunal density at Incheon Harbor. Incheon Harbor was characterized by high abundance of H. filiformis. Compared with the previous studies carried out in Kyeonggi Bay, faunal density and species number have little changed for the recent 10 years. Dominant species was different between each study, but has been among opportunistic species of small-sized polychaetes belonging to capitellids, cirratulids or spionids. These results indicate that Kyeonggi Bay has been organically polluted steadily; specifically, this study reveals that Incheon Harbor has been the center of pollution and that the benthic community in the outer sea off Sihwa dike has become unstable.

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Molecular Cloning and Analysis of Nucleotide Sequence of Xylanase Gene (xynk) from Bacillus pumilus TX703 (Bacillus pumilus TX703 유래 Xylanase 유전자(xynK)의 Cloning과 염기서열 분석)

  • 박영서
    • Journal of Life Science
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    • v.12 no.2
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    • pp.188-199
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    • 2002
  • A gene coding for xylanase from thermo-tolerant Bacillus pumilus TX703 was cloned into Escherichia coli DH5 $\alpha$ using pUC19. Among 7,400 transformants, four transformants showed clear zones on the detection agar plates containing oat-spells xylan. One of them which showed highest xylanase activity was selected and its recombinant plasmid, named pXES106, was found to carry 2.24 kb insert DNA fragment. When the nucleotide sequence of the cloned xylanase gene (xynK) was determined, xynK gene was found to consist of 1,227 base-pair open reading frame coding for a polypeptide of 409 amino acids with a deduced molecular weight of 48 kDa. The coding sequence was preceded by a putative ribosome binding site, the transcription initiation signals, and cia-acting catabolite responsive element. The deduced amino acids sequence of xylanase is similar to those of the xylanases from Hordeum vulgare (barley) and Clostridium thermocellum, with 39 and 31% identical residues, respectively. The amino acids sequence of this xylanase was quite different from those of the xylanases from other Bacillus species.

Reconfirmation of age and growth of the pointhead flounder, Hippoglossoides pinetorum in the coastal waters of the East Sea off Gyeongbuk (경북 동해안산 용가자미 (Hippoglossoides pinetorum) 연령과 성장의 재확인)

  • JEONG, Yeon Kyu;KIM, Sung Tae;YOON, Sang Chul;YANG, Jae Hyeong;JUNG, Kyung Mi;OH, Taek Yun;CHOI, Kwang Ho
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.53 no.4
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    • pp.363-375
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    • 2017
  • The age and growth of pointhead flounder, Hippoglossoides pinetorum caught by gill nets was analyzed in this study from March 2015 to July 2017. New annuli were formed in H. pinetorum otoliths annually, and the boundary was set between the opaque and translucent zones from March and April. The relationships between total length (TL) and body weight (BW) were $BW=0.0025TL^{3.409}$ ($r^2=0.9551$) for females and $BW=0.0057TL^{3.138}$ ($r^2=0.9163$) for males. In this study, the ring of pointhead flounder, H. pinetorum was formed between 3 and 8 for females and between 3 and 6 for males. Total length (TL) and otolith radius (OR) were measured as follows: TL = 7.142 OR + 0.769 ($r^2=0.793$) for females and TL = 6.498 OR + 1.706 ($r^2=0.652$) for males. The mean distances of first ring ($r_1$) were 0.92 mm and 0.91 mm for females and males respectively. The TLs at the time of annulus formation, back-calculated from the otolith-length relationship by reference to the von Bertalanffy growth curves, were $L_t=43.59(1-e^{-0.15(t+0.007)})$ for females and $L_t=28.13(1-e^{-0.26(t+0.006)})$ for males while the growth between female and male was different.

Antimicrobial and antioxidant activity of some Indian medicinal plants for the protection against fish pathogenic bacteria

  • Harikrishnan, Ramasamy;Jawahar, Sundaram;Kim, Man-Chul;Kim, Ju-Sang;Jang, Ik-Soo;Balasundaram, Chellam;Heo, Moon-Soo
    • Journal of fish pathology
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    • v.22 no.3
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    • pp.317-326
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    • 2009
  • This study has shown the screening of anti-bacterial activity of three Indian medicinal plant choloroform : methanol (50:50) solvent leaf extracts (i.e. Azadirachta indica, Ocimum sanctum, and Curcuma longa) with different concentrations (10, 5, 2.5, 1.25, 0.625, 0.312, and 0.156 mg/ml) under in vitro conditions against fish pathogenic bacteria, Aeromonas hydrophila, Streptococcus iniae, Vibrio harveyi, V. anguillarum, and Edwardsiella tarda isolated from olive flounder farms, Jeju Island, South Korea. The anti-microbial activity of the A. indica and O. sanctum extracts yielded the zones of growth inhibition (ZI) was 3 and 1mm against A. hydrophila at concentration of 0.156 mg/ml when compared to that of tetracycline standard (3 mm). At highest concentration (10 mg/ml) of A. indica, O. sanctum, and C. longa, high inhibition was 9, 7, and 6 mm when compared to that of tetracycline (11 mm) against A. hydrophila. The minimum inhibitory concentration (MIC) of A. indica, O. sanctum, and C. longa at 0.156 mg/ml that yield 9, 10, and 13 CFU/ml for A. hydrophila, 16, 22, and 25 CFU/ml for S. iniae and 18, 22, and 23 CFU/ml for E. tarda compared to the tetracycline. At highest concentration (10 mg/ml) of the three extracts was better inhibiting the growth of A. hydrophila, S. iniae and E. tarda. A. indica, O. sanctum, and C. longa were determined to the potential antioxidant activityon the basis of their scavenging activity of the stable 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical. A. indica extract was 0.625 mg/ml which indicated that the strong anti-oxidant activity. However, O. sanctum and C. longa extracts showed weak anti-oxidant activity at this concentration. Hence, in vitro assay among the pathogens, A. hydropila is better inhibitory activity of the extracts. It is evident that the Indian medicinal plants extracts were subjected to its effectiveness against A. hydrophila, S. iniae, and E.tarda at low concentrations. The obtained results in the present study suggested that the Indian plant extracts is a prevention tools for Korean olive flounder aquaculture pathogens and its need further advance investigation.

Analysis on the Occurrence of Rice stripe virus (벼줄무늬잎마름병의 발생 분석)

  • Lee, Bong-Choon;Yoon, Young-Nam;Hong, Sung-Jun;Hong, Yeon-Kyu;Kwak, Do-Yeon;Lee, Jong-Hee;Yae, Un-Sang;Kang, Hwang-Won;Hwang, Hung-Goo
    • Research in Plant Disease
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    • v.14 no.3
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    • pp.210-213
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    • 2008
  • The occurrence of Rice stripe virus (RSV) has been confirmed in some parts of South Korea. In the current year, this was observed to be limited in the double cropping zones of the southern region. Earlier, RSV occurred largely in the west coast regions of the country, particularly in Gangwha, Gyeonggi-do in 2001; and Buan, Jeollabuk-do and Seocheon, Chungcheongnam-do in 2007. This study was carried out to determine the nationwide extent of RSV occurrence by investigating RSV infestations in different latitudes and altitudes. Result revealed a recent reported occurrence of RSV in the southern region (Milyang, latitude $35^{\circ}$), a reported occurrence in the northern region (Cheolwon, latitude $38^{\circ}$) in 2005, and in the middle northern region of the east coast (Goseon, latitude $38^{\circ}$) as newly confirmed from this investigation. Results also confirmed the occurrence of RSV in a wide range of altitude from the plains (Milyang, 17 m altitude) to the alpine regions (Jinbu, 576 m) including the middle mountains (Sangju, altitude 285 m). The RSV occurrence in Jinbu in the alpine region and at Goseong and most of the northern end regions were confirmed only this year. The results of the present study confirmed that RSV is a fast-spreading disease in rice plant cultivation regions of the entire country.

Determination of Optimal Hourly Water Intake Amount for H Arisu Purification Center using Linear Programming (선형계획법을 이용한 H 아리수 정수 센터 최적 취수량 결정)

  • Lee, Chulsoo;Lee, Kangwon
    • Journal of Korea Water Resources Association
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    • v.48 no.12
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    • pp.1051-1064
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    • 2015
  • Currently, the H purification plant determines the hourly water intake amount based on operator experience and skill. Therefore, inevitably, there are deviations among operators. While meeting time-varying demand and maintaining the proper water level in the clean water reservoir, the methodology for minimizing electricity cost, when dealing with different electricity rate time zones, is a very complicated problem, which is beyond an operator's capability. To solve this problem, a linear programming (LP) model is proposed, which can determine the optimal hourly water intake amount for minimizing the daily electricity cost. It is shown that an inaccurate estimate for the hourly water usage in the demand areas causes the water level constraint to be violated, which is the weak point of the proposed LP method. However, several examples with real-field data show that we can practically and safely solve this problem with safety margins. It is also shown that the safety margin method still works effectively whether the estimate is accurate or not. The operators need not attend the site at all times under the proposed LP method, and we can additionally expect reductions in labor costs.

Spatial-temporal Variations of Nitrate Levels in Groundwater of Jeju Island, Korea: Evaluation of Long-term (1993-2015) Monitoring Data (제주도 지하수질산염 농도의 시·공간적변화 특성: 장기(1993-2015) 모니터링 자료의 평가)

  • Kim, Ho-Rim;Oh, Junseop;Do, Hyun-Kwon;Lee, Kyung-Jin;Hyun, Ik-Hyun;Oh, Sang-Sil;Kam, Sang-Kyu;Yun, Seong-Taek
    • Economic and Environmental Geology
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    • v.51 no.1
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    • pp.15-26
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    • 2018
  • The spatio-temporal variations of nitrate concentrations in groundwater of Jeju Island were evaluated by an analysis of time series groundwater quality data (N = 21,568) that were collected from regional groundwater monitoring (number of wells = 4,835) for up to 20 years between 1993 and 2015. The median concentration of $NO_3-N$ is 2.5 mg/L, which is slightly higher than those reported from regional surveys in other countries. Nitrate concentrations of groundwater in wells tend to significantly vary according to different water usage (of the well), administrative districts, and topographic elevations: nitrate level is higher in low-lying agricultural and residential areas than those in high mountainous areas. The Mann-Kendall trend test and Sen's slope analysis show that nitrate concentration in mid-mountainous areas tends to increase, possibly due to the expansion of agricultural areas toward highland. On the other hand, nitrate concentrations in the Specially Designated Groundwater Quality Protection Zones show the temporally decreasing trend, which implies the efficiency of groundwater management actions in Jeju. Proper measures for sustainable groundwater quality management are suggested in this study.

Modeling of Hemodynamics in Stenosed Artery (협착 동맥혈관의 혈류유동 모델링)

  • Kim, Seong-Jong;Park, Young-Ran;Kim, Shagn-Jin;Kang, Hyung-Sub;Kim, Jin-Shang;Oh, Sung-Hoon;Kang, Sung-Jun;Kim, Gi-Beum
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.13 no.5
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    • pp.2285-2290
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    • 2012
  • This study is about atherosclerosis which occupies the highest rate in many diseases people have and we have studied about atherosclerosis for abdominal aorta. Atherosclerosis is the phenomenon which blood vessel gets narrower, harder and thicker due to the stenosis of colesterol in blood vessel. If it becomes worse, arteries will be hard and blood can't flow smoothly, and even it can reach to death. In this study, the geometric models of the considered stenotic blood flow are two different types of constriction of cross-sectional area of blood vessel; 20 and 45% of constriction in each elastic wall and rigid wall. We have modeled by using finite element method to observe the changes of velocity and pressure. In case of the diameter of blood vessel decreased 45% in elastic wall model, the values of velocity and pressure were higher than the case of 20% and in case of the diameter of blood vessel decreased 45% in rigid wall model, the values of velocity and pressure were higher than the case of 20%. In cases of elastic wall models of the diameters of blood vessels decreased each of 20% and 45%, recirculation zones appeared. This results show understanding of hemodynamic properties depending on stenosed blood vessels.

An Experimental Study on the Fire Monitoring System for Tunnel Using SMA and Fiber Optic Cable (형상기억합금과 광케이블을 이용한 터널의 화재감지 시스템 개발에 관한 실험적 연구)

  • Hwang, Ji-Hyun;Park, Ki-Tae;Lee, Kyu-Wan
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.18 no.5
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    • pp.128-134
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    • 2014
  • Recently, design and construction of street tunnels tend to focus on cost reduction and preservation of nature. Accordingly, research is actively being carried out to quickly detect fires when they occur in tunnels, which have partially closed structures. Among such research, fire detection methods using optical fiber sensors have a wide bandwidth and fast transmission speed, while using light as a medium. Therefore, it does not receive electrical interference and there is almost no loss of information during transmission, while also having little noise as well. In relation to this, a fire monitoring system that can accurately detect the location of fires in real time using shape memory alloy and optical cables was developed in this study. In order to verify the developed method, light loss measurement test was conducted according to indoor temperature changes, while also conducting fire simulation tests by installing test beds in common underground zones with different external environments of temperature and distance. Upon carrying out experiments, the fire monitoring system developed in this study was found to be able to detect fires in long distance sections in real time.

Phylogenic Study of Genus Asarum (Aristolochiaceae) in Korea by trnL-trnT Region (trnL-trnT 부위에 의한 한국 족도리풀속 식물종의 계통분류학적 연구)

  • Lee, Byeong-Ryong;Kim, Seon-Hoan;Huh, Man-Kyu
    • Journal of Life Science
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    • v.20 no.11
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    • pp.1697-1703
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    • 2010
  • Asarum consists of low-growing herbs and is a genus in the Aristolochiaceae family with species found in the north temperate zones with most species in Asia. We evaluated the nine taxa with the trnL - trnT region of the chloroplast genome to estimate phenotypic relationships within genus Asarum in Korea. Alignment of the DNA sequences required the addition of numerous gaps. Sequence variation within the Asarum was mostly due to nucleotide inserts/deletions, although several indels and inserts were found. Another source of sequence divergence was length variation due to stretches of short repeats that occur at the trnL - trnT region in all the Asarum. A + T content for nine Korean species of genus Asarum ranged between 74.7% and 78.3%. These values were higher than those for the angiosperm alignments of the total trnL and trnT region (64.5~67.1%). Within genus Asarum, A. patens was strikingly different from the others in the three phylogenetic analyses (MP, ML, and NJ). However, some internal nodes were poorly supported. Within Korean Asarum, four species were unsolved portions. Possible reasons for the striking non-congruence between the previous morphological traits and the trnL - trnT based on phylogeny were discussed.