• Asian-Australasian Journal of Animal Sciences
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• v.24 no.9
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• pp.1256-1267
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• 2011

Two experiments were conducted to determine the effects of dietary lysine and energy level on performance and apparent total tract digestibility (ATTD) of nutrients in weanling pigs. In Exp. 1, a total of 288 weaned pigs (initial BW $5.77{\pm}0.85\;kg$) were randomly allotted to 4 treatments (4 replicates per treatment with 18 pigs in each replicate). Experimental diets were fed in the 3 phases: phase I (d 0 to 7), phase II (d 8 to 14) and phase III (d 15 to 28). Isocalorific diets (3,450 kcal/kg) with incremental lysine levels (Phase I: 1.51, 1.61, 1.71 and 1.81; Phase II: 1.35, 1.46, 1.56 and 1.66; Phase III: 1.18, 1.28, 1.39 and 1.49% lysine respectively for T1, T2, T3 and T4) were used as treatments. An increase in the dietary lysine levels linearly improved (p<0.05) the ADG and G:F during phases I, II and III as well as overall study period. The ATTD of DM (d 7 and 28) and CP (d 7, 14 and 28) were linearly improved (p<0.05) with increasing dietary lysine levels. ATTD of ash, Ca and P were not affected by dietary lysine level. In Exp. 2, 64 weanling pigs (initial BW $4.79{\pm}0.79\;kg$) were randomly allotted to 4 treatments (4 replicates per treatment with 4 pigs in each replicate) in a $2{\times}2$ factorial arrangement on the basis of BW. Effects of two levels of energy (high, 3,450 or low, 3,350 kcal/kg) and lysine (high or low; 1.70 or 1.50, 1.55 or 1.35 and 1.40 or 1.20% in phase I, II and III diets, respectively) on performance and ATTD of nutrients were investigated. High energy and lysine diets improved ADG (p<0.05) in pigs during phase I, II and III and overall period (p<0.001), while G:F increased (p<0.05) during phase I and overall period. Pigs fed high lysine diets consumed more (p<0.05) feed during phase III and overall period. Additionally, pigs fed high energy diets had greater (p<0.05) ATTD of GE (d 7 and 14), CP (d 7 and 28) and DM (d 28); whereas, pigs fed high lysine diets had greater (p<0.05) ATTD of GE and CP during d 7, 14 and 28. ATTD of ash, Ca and P remained unaffected (p>0.05) by dietary energy and lysine level. However, there was no energy${\times}$lysine interaction for any of the measured variables. Results obtained in present study suggested that high energy and lysine level improve the growth performance and ATTD of nutrients in weanling pigs.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.1
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• pp.77-81
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• 2001

The effects of dietary protein and lysine levels on lactating multiparous sows and litter performance were studied. Sixty-two crossbred multiparous sows ($Landrace{\times}Yorkshire$) were used. Thirty-three and twenty-nine sows were studied in their second parity and third parity respectively. The three dietary treatments were: (1) the control diet containing 15% CP and 0.75% lysine, (2) a diet containing 13% CP and 0.75% lysine (0.60% natural+0.15% synthetic), and (3) a diet containing 13% CP and 0.60% lysine. They were fed twice daily and allowed ad libitum access to food and water throughout a 28 day lactation from parturition until weaning. The results of this experiment showed that body weight and backfat losses of the sows from farrowing to weaning were significantly affected (p<0.01) by reducing dietary protein. Neither average daily feed intake nor weaning to estrus interval of sows were significantly different among treatments. Supplementing lower dietary protein with synthetic lysine could mitigate backfat losses, but could not prevent body weight losses in lactating multiparous sows. A corn-soybean meal diet containing 13% crude protein and 0.60% lysine did not significantly affect litter size and survival rate of weanling piglets compared with the 15% crude protein diet. There was a tendency towards decreased piglet weight at weaning (p<0.10) and reduced daily gain of piglets (p<0.11) when the multiparous sows were fed the 13% protein diet during lactation. We found a severe loss of body weight and backfat when reducing dietary protein for lactating multiparous sows.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.6
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• pp.923-931
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• 1999

An experiment was conducted to determine the effect of lysine on performance and carcass composition of broilers under heat stress during the grower period (3-6 weeks). A factorial arrangement of three levels of dietary protein (18, 20, and 22%), three levels of dietary lysine (1.26, 1.39, and 1.52%), and two rearing temperature regimens were used in this study. Birds were kept under either moderate temperature ($24{\pm}1^{\circ}C/24h$) or hot cycling temperature ($26-34^{\circ}C/6h$, $34{\pm}1^{\circ}C/12h$, and $34-26^{\circ}C/6h$). Body weight (BW), weight gain (WG), feed intake (FI), feed conversion (FE), carcass weight (CW), carcass yield (CY), and percentages of breast meat (BM), abdominal fat (AF), drumsticks (DS), and thighs (TH) were determined at the end of experiment. Exposure to high ambient temperature significantly (p<0.05) decreased BW, WG, FI, FE, CW, BM, AF, and increased CY, DS, and TH. High dietary protein significantly (p<0.05) decreased AF and TH, and improved CW only under moderate temperature, resulting in significant (p<0.05) protein by temperature interaction. High dietary lysine significantly (p<0.05) decreased BW, WG, FI, CW, CY and AF, while BM was reduced only when high dietary protein was fed, resulting in significant (p<0.05) protein by lysine interaction. It is concluded that increasing dietary lysine adversely affected broilers' performance and carcass composition irrespective of rearing temperature.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.12
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• pp.1731-1737
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• 2000

This study was conducted to investigate the effects of threonine:lysine ratios on growth performance, apparent nutrient digestibility and blood urea nitrogen (BUN) concentration, and to estimate the optimal threonine:lysine ratios for growing barrows and gilts. A total of 150 pigs (Landrace${\times}$Yorkshire${\times}$Duroc, $16.75{\pm}0.42kg$ average body weight, 75 barrows and 75 gilts) was randomly allotted into six treatments in a $2{\times}3$ factorial design. Six diets were formulated to contain 1.12% lysine for barrows and 1.33% lysine for gilts with three threonine:lysine ratios (50, 60 and 70%) for both barrows and gilts. Throughout the whole experimental period (16 to 56 kg body weight), there was no interaction between sex and dietary threonine:lysine ratio in average daily gain (ADG), average daily feed intake (ADFI) and feed conversion rate (FCR). Between sexes, there was a clear sex-effect showing better growth performance of barrows. Barrows consumed more feed (p<0.01) and grew faster (p<0.01) than gilts. For barrows, there was a trend to improved ADG and FCR with increasing threonine:lysine ratio. For gilts, there was a trend to improved ADG and FCR up to threonine:lysine ratio of 60%, but not significant. There was no interaction between sex and threonine:lysine ratio in nutrient digestibilities of growing pigs except for crude ash (CA). Between sexes, there were differences in nutrient digestibilities, except for calcium for which gilts showed higher a digestibility (p<0.01). Among dietary threonine:lysine ratios, there were no differences in nutrient digestibilities. Mean values of essential amino acids (EAA), non-essential amino acids (NEAA) and total amino acids (TAA) digestibilities were not affected by sex and dietary threonine:lysine ratio. There was no evidence of an interaction between sexes and dietary threonine:lysine ratio. Between sexes, total BUN concentration was lower in gilts than barrows (p<0.05). It was concluded that a 70 and 60% dietary threonine:lysine ratio for barrows (1.12% lysine) and gilts (1.33% lysine) tended to result in better growth performances and nutrient utilization and lower BUN concentration than other threonine:lysine ratios.

• Journal of agriculture & life science
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• v.50 no.5
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• pp.129-137
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• 2016

This study investigated the effect of dietary lysine and gamma-linolenic acid(GLA) levels on growth performance, carcass traits, and meat quality in finishing pigs. Pigs were provided with feed containing two different levels of lysine(0.45% and 0.75%) with three different levels of gamma-linolenic acid(0.0, 0.3, and 0.6%). Average daily gain(ADG) was significantly lower (p<0.01) in pigs provided with the lower level of lysine. In contrast, feed/gain(p<0.01), diet cost/gain(p<0.05), and intramuscular fat(p<0.01) were all significantly higher in pigs fed the lower level of lysine. Similarly, meat color scores(CIE L*, a*, and b*) and cooking loss were significantly higher(p<0.01) in pigs fed the lower level of lysine, whereas shear force(kg/2.5 inch2)was not affected by dietary lysine. The addition of GLA had no significant effect on any of the parameters measured. The results indicate that providing pigs with 0.45% lysine in their diet may help to increase intramuscular fat content, allowing the industry to produce pork products that meet consumer needs in Korea.

• Asian-Australasian Journal of Animal Sciences
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• v.5 no.1
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• pp.129-138
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• 1992

The effects of dietary levels of lysine and energy on growth performance, the content of DNA, RNA and protein in liver, thigh muscle composition and nutrient utilization in broiler chicks were investigated in an experiment involvies with 2 levels of dietary energy : 3,200 (2900) 2,900 (2700) kcal ME/kg) and 6 levels of lysine : 0.6(0.5), 0.8(0.7), 1.0(0.9), 1.2(1.1), 1.4(1.3), and 1.6(1.5)% was carried out. A total number of 384 male broiler chicks was used for a period of 7 weeks. Body weight gain of 1.0(0.9)% lysine level group was significantly (p < 0.01) higher than that of any other groups. Interaction between lysine and energy in the feed intake was observed (p < 0.05). Present data indicate that the content of DNA in liver tissues was significantly (p < 0.05) different by the levels of lysine, namely, 1.0(0.9)% or 1.2(1.1)% lysine level groups showed higher content than other groups (p < 0.01). Dietary levels of 1.2(1.1)% or 1.6(1.5)% lysine groups showed the highest protein content in thigh muscle tissues than that of any other groups (p < 0.05). Interaction between energy and lysine in the content of protein of thigh muscle tissues was shown (p < 0.01). The level of 0.6% lysine group showed the highest fat content in thigh muscle tissues than any other groups. Interaction between lysine and energy in the content of crude ash and crude fat of thigh muscle tissues was observed (p < 0.01). Apparent amino acid availability of arginine, glycine and threonine (p < 0.01), phenylalanine (p < 0.05) were significantly affected by the levels of lysine and interaction between lysine and energy was found only in arginine (p < 0.01).

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.7
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• pp.1003-1011
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• 2013

Two experiments were conducted to determine the effects of different dietary lysine levels on the apparent nutrient digestibility, the serum amino acid (AA) concentration, and the biochemical parameters of the precaval and portal vein blood in growing pigs. In Experiment 1, 15 noncannulated pigs received diets with different lysine densities (0.65%, 0.95%, and 1.25% lysine) for 13 d. A total collection digestion test was performed, and blood samples were collected from the precaval vein at the end of the experiment. In Experiment 2, four cannulated pigs were fed the same diets of Experiment 1. The experiment used a self-control experimental design and was divided into three periods. On d 5 of each period, at 0.5 h before feeding and hourly up to 8 h after feeding, single blood samples were collected from catheters placed in the portal vein. In Experiment 1, some serum AAs (including lysine), serum urinary nitrogen (SUN), and total protein (TP) concentrations were significantly affected by the dietary lysine levels (p<0.05). Moreover, the 0.65% lysine treatment showed a significant lower apparent digestibility of gross energy, dry matter, crude protein, and phosphorus than the other treatments (p<0.05). In Experiment 2, serum lysine, histidine, phenylalanine, threonine, valine, isoleucine (p = 0.0588), triglyceride, and SUN (p = 0.0572) concentrations were significantly affected by the dietary lysine levels (p<0.05). Additionally, almost all of the determined serum AA and total AA concentrations reached their lowest values at 0.5 h before feeding and their highest values at 2 h after feeding (p<0.05). These findings indicate that the greatest absorption of AA occurred at 2 h after feeding and that the dynamic profile of serum AA is affected by the dietary lysine levels. Moreover, when the dietary lysine content was 0.95%, the growing pigs achieved a better nutrient digestibility and serum metabolites levels.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.7
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• pp.1100-1107
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• 2007

The effects of offering broilers phosphorus-adequate diets containing 10.0 and 11.8 g/kg lysine, without and with 500 FTU/kg exogenous phytase, on growth performance and nutrient utilisation were determined. Each of the four experimental diets was offered to 6 replicates of 10 birds from 7 to 28 days of age. Effects of treatment on performance, apparent metabolisable energy, apparent ileal digestibility of amino acids and bone mineralisation were examined. Both additional lysine and phytase supplementation improved (p<0.05) weight gain and feed efficiency, with interactions (p<0.05), as phytase responses were more pronounced in lysine-deficient diets. Phytase improved (p<0.05) apparent metabolisable energy, which was independent of the dietary lysine status. Bone mineralisation, as determined by percentage toe ash, was not affected by treatment, which confirms the phosphorus-adequate status of the diets. Phytase increased (p<0.05) the apparent ileal digestibility of the sixteen amino acids assessed. Unexpectedly, however, the dietary addition of 1.8 g/kg lysine, as lysine monohydrochloride, increased (p<0.05) the ileal digestibility of lysine per se and also that of isoleucine, methionine, phenylalanine, valine, aspartic acid, glutamic acid and tyrosine. In addition, there were significant interactions (p<0.05) between additional lysine and phytase supplementation for arginine, lysine, phenylalanine, aspartic acid, glutamic acid, glycine and serine digestibilities, with the effects of phytase being more pronounced in lysine-deficient diets. The possible mechanisms underlying the increases in amino acid digestibility in response to additional lysine and the interactions between lysine and microbial phytase in this regard are discussed. Also, consideration is given to the way in which phytate and phytase may influence ileal digestibility of amino acids.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.4
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• pp.568-572
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• 2006

The effect of dietary lysine supplementation on the performance of crossbred lactating sows (n = 49, total 92 litters) and suckling piglets during the warm and cool seasons was studied. Sows were randomly allocated to one of two experimental diets, which were fed throughout a 28-day lactation period. Two diets were formulated based on corn-soybean meal with 6% wheat bran, containing 15% crude protein, and with or without 0.25% lysine-HCl supplementation. No interaction was observed between season and dietary lysine supplementation. Dietary lysine supplementation significantly (p<0.05) increased weaning weight (7.11 vs. 6.46 kg) and daily gain (0.20 vs. 0.18 kg) of piglets. During the warm season lactating sows had significantly lower feed intake (3.78 vs. 6.11 kg, p<0.01), higher weight loss (19.81 vs. 9.73 kg, p<0.01) and backfat loss (0.23 vs. 0.06 cm, p<0.01), longer interval from weaning to estrus (9.32 vs. 6.21 days, p<0.05), lower litter weight gain (42.13 vs. 52.90 kg, p<0.01) and lower daily gain (0.17 vs. 0.21 kg, p<0.01) than lactating sows during the cool season. The results indicate that the influences of dietary treatment and season were independent. The 15.0% CP lactation diet with 0.25% lysine-HCl supplementation did not improve the performance of lactating sows and litter piglets, which was impaired by the warm season.

• Fisheries and Aquatic Sciences
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• v.19 no.1
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• pp.4.1-4.7
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• 2016

This study evaluated the dietary lysine requirement by measuring the plasma free lysine concentrations in rainbow trout, Oncorhynchus mykiss after dorsal aorta cannulation. A basal diet containing 36.6 % crude protein (29.6 % crystalline amino acids mixture, 5 % casein and 2 % gelatin) was formulated to one of the seven L-amino acid based diets containing graded levels of lysine (0.72, 1.12, 1.52, 1.92, 2.32, 2.72 or 3.52 % dry diet). A total of 35 fish averaging $512{\pm}6.8g$ ($mean{\pm}SD$) were randomly distributed into seven groups with five fish in each group. After 48 h of feed deprivation, each group of fish was fed one of the experimental diets by intubation at 1 % body weight. Blood samples were taken at 0, 5 and 24 h after intubation. Post-prandial plasma free lysine concentrations (PPlys, 5 h after intubation) of fish fed diets containing ${\geq}2.32%$ lysine were higher than those of fish fed diets containing ${\leq}1.92%$ lysine. Post-absorptive free lysine concentrations (PAlys, 24 h after intubation) of fish fed diets containing 2.32 and 3.52 % lysine were higher than those of fish fed diets containing ${\leq}1.52%$ lysine. The brokenline regression analysis on the basis of PPlys and PAlys indicated that the lysine requirement of rainbow trout could be 2.34 and 2.20 % in diet. Therefore, these results strongly suggested that the dietary lysine requirement based on the broken-line model analyses of PPlys and PAlys could be greater than 2.20 % but less than 2.34 % (corresponding to be $6.01%{\leq},but{\leq}6.39%$ in dietary protein basis, respectively) in rainbow trout. Also, these results shown that the quantitative estimation of lysine requirement by using PPlys and PAlys could be an acceptable method in fish.