• Asian-Australasian Journal of Animal Sciences
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• v.23 no.11
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• pp.1490-1495
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• 2010

Two experiments were conducted to evaluate the effects of addition of sodium polyacrylate (SPA) to a phytasesupplemented diet on the performance and phosphorus (P) retention of chicks. In experiment 1, chicks were randomly allocated to four dietary treatments which were fed from 7 to 21 days of age: i) basal diet (low nonphytate phosphorus (0.23% NPP)); ii) basal with 250 U/kg diet of phytase; iii) as (ii) with 2.5 g/kg diet of SPA; and iv) as (ii) with 5.0 g/kg diet of SPA. In experiment 2, three replicates, each with three chicks, were fed from 7 to 28 days of age the basal diet (0.23% NPP) with supplementation of phytase (0, 300, 600, 900 U/kg diet) and SPA (0, 2.5 g/kg diet) in a $4{\times}2$ factorial arrangement. In Experiment 1, feed efficiency was improved and excreted P was 10% less with phytase supplementation. However, the addition of SPA did not affect performance or P excretion. Dietary SPA supplementation to the diets showed significantly higher amounts of P retention, and highest values were observed in chicks fed 2.5 g/kg of the SPA-supplemented diet. In Experiment 2, feed efficiency was improved with phytase supplementation, and the addition of SPA showed significant improvement in feed efficiency. Excreted P was significantly lower in chicks fed SPA-supplemented diets, and the retained P coefficient improved with SPA supplementation. In conclusion, the increased transit time of digesta with suitable supplementation levels of SPA may allow phytase activity to be more effective in the degradation of phytate, and improve P retention.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.7
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• pp.994-998
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• 2017

Objective: An experiment was conducted to determine the effect of superdosing phytase on productive performance and egg quality in laying hens. Methods: A total of 200 42-wk-old Hy-Line Brown laying hens were allotted into 1 of 5 dietary treatments with 5 replicates consisting of 8 hens per replicate. The positive control (PC) and negative control diets (NC) were prepared based on the recommended P levels in layer diets. Supplemental phytase was added to the negative control diet at 10,000 (SD10), 20,000 (SD20), or 30,000 (SD30) fytase units (FTU)/kg. Productive performance was summarized for 6 weeks from 42 weeks to 47 weeks of age. Egg quality was assessed from 4 eggs per replicate randomly collected at the conclusion of the experiment. Results: The SD20 treatment had greater (p<0.05) hen-day egg production than PC, NC, and SD10 treatment groups. There was no difference in hen-day egg production between SD20 and SD30 treatment groups. However, SD30 treatment had greater (p<0.05) hen-day egg production than PC treatment, but showed no difference in hen-day egg production as compared to NC and SD10 treatment groups. However, egg weight, egg mass, feed intake, and feed conversion ratio were not affected by dietary treatments. Egg quality including eggshell strength, eggshell color, egg yolk color, and haugh unit was not influenced by dietary treatments. Conclusion: Superdosing level of 20,000 FTU/kg phytase in diets has a positive effect on egg production rate, but no beneficial effect on egg quality in laying hens.

• Journal of Animal Science and Technology
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• v.44 no.1
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• pp.55-60
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• 2002

Two experiments were conducted to investigate the effect of dietary supplementation of plant phytase (Phytazyme$^{(R)}$) in corn-soybean meal based diets on utilization of phytase-bound phosphorus in laying hens and evaluate nitrogen(N) digestibility and phosphorus(P) availability in breeders. In the experiment one, three levels of the Phytazyme$^{(R)}$(0.05, 0.1, and 0.2% of diet) were added to diets containing a half of control TCP level(0.96%) for 4 wks. Feed consumption, egg production rate, egg weight and eggshell quality were recorded weekly. At the end of experiment, 8 birds per treatment were sacrificed, liver weight were weighed and right tibiae were removed for determination of P content. The second experiment was conducted to evaluate the P availability and nitrogen digestibility in breeders fed same diets for 2 wks. Feed and excreta were collected to determine the P and N contents for the last three days of experiment two. Addition of Phytazyme$^{(R)}$ resulted in no effects on feed intake, egg product rate, egg weight and egg shell quality. P excretion decreased and its availability enhanced as phytase supplementation increased in diets. Dietary supplementation of Phytazyme$^{(R)}$ above 0.1% level in corn-soybean meal based diets did not have an adverse effect on production and decreased level of phosphorus in excreta.

• International Journal of Human Ecology
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• v.4 no.1
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• pp.25-34
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• 2003

High dietary phytate is a known factor in reducing the bioavailability of minerals such as zinc and calcium which are already chronically low in the Korean diet. This study was conducted to develop methods for reducing dietary phytate through the addition of phytate and/or the substitution of high phytate foods with low phytate foods. Ten units of phytase per 100g of uncooked brown rice were added to brown rice gruel resulted in a 16.2% phytate reduction after a 3-hour incubation period; an 18.2% reduction was produced after a 6-hour incubation period. The addition of ten units of phytase per 100g of soybean curd residue at 45$^{\circ}C$, followed by refrigeration for 3 hours, resulted in a 19.1% phytate reduction. The addition of 20 units of phytase under the same conditions reduced phytate content by 24.6%. In this study, two typical Korean meals consisting of legumes and unrefined cereals were prepared as high phytate meals; these were then compared to low phytate meals that had been prepared by treating the foods with phytase and substituting unrefined with refined cereals (i.e., brown rice with white rice, whole wheat bread with white bread). The phytate content of the two high phytate meals was 1878.2mg and 1811.8mg. After the addition of phytase and the food substitution, the phytate content of the low phytate meals was reduced to 788.9mg and 606.0mg. The phytate to zinc molar ratio of high phytate diets was 22.4 and 21.3 and 9.4 and 7.9 for the low phytate meals. These results indicate that the nutritional status of Koreans in terms zinc and other minerals can be improved by phytate reduction. This can be accomplished through the change of milling process for some cereals and/or the enzyme treatment of some high phytate food items.

• Journal of Animal Science and Technology
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• v.66 no.1
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• pp.93-102
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• 2024

This study investigated the effect of dietary supplementation with phytase on growth performance, fecal excretion, and compost nutrition on broilers fed available phosphorus (avP)- and calcium (Ca)-deficient diets. A total of 750 one-day-old broiler chicks were randomly divided into five dietary groups having ten replications in a floor house. Diets of the groups were formulated with positive control (PC), negative control (NC; low avP and Ca), and NC supplemented with phytase levels; 500 (NC500), 1,000 (NC1000), and 1,500 FTU/kg (NC1500). A three-phase feeding program was used in the trial. Average daily gain (ADG) and average daily feed intake (ADFI) in the groups fed diets supplemented with phytase were significantly (p < 0.05) higher than those fed NC and the increase was equivalent to those fed PC. Serum levels of Ca and phosphorus (P) were higher (p < 0.05) in broilers fed NC1000 and NC1500 than in those fed NC. Interleukin (IL) level was the lowest in the group fed NC. Plasma myo-inositol (INS) concentrations in the NC1500 group were higher (p < 0.05) than PC, NC, and NC500 groups. Crude protein (CP) excretion was notably (p < 0.05) lower in the NC1500 group than in PC and NC groups. A lower (p < 0.05) concentration of P₂O₅ was observed in compost from the group fed NC1500 than the groups fed PC and NC. Accordingly, we suggest that phytase supplementation in lower avP and Ca levels of broiler diet can improve their productive performance and reduce environmental pollution.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.6
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• pp.824-829
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• 2000

A five wk feeding experiment was conducted with day-old one thousand broiler chicks (Arbor Acres) to determine the effects of microbial phytase (Natuphos$^{(R)}$) supplemented to low nonphytate P (NPP) corn-soy diets. Five pens of 50 mixed sex birds each were randomly assigned to each of the four dietary treatments: T1, control diet containing normal NPP level; T2, T1-0.1% NPP+600 U of phytase/kg diet; T3, T1 - 0.2% NPP+600 U of phytase/kg diet; and T4, T1 - 0.3% NPP+600 U of phytase/kg diet. T1, T2, and T3 showed similar growth rate, feed intake, and feed efficiency, indicating that NPP level in broiler diets could be reduced by approximately 0.2% by the microbial phytase supplementation. But T4 showed significantly (p<0.05) lower weight gain than others. The phytase supplementation improved P availability resulting in low P excretion. Weight and girth of metatarsal bone were increased by phytase supplementation at low NPP diet treatments but ash contents were not significantly different. It can be concluded that NPP level of corn-soy broiler diets can be safely lowered by approximately 0.2 % by supplementing 600 U of microbial phytase/kg diet. With the adjusted level of NPP and phytase supplementation, P excretion could be reduced by 50%.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.2
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• pp.198-202
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• 2006

An experiment was conducted to investigate the effect of phytase, organic acids and their interaction on body weight gain, feed consumption, feed conversion ratio, carcass yield and tibia ash. A total of 680 three-day old Japanese quail chicks (Coturnix coturnix japonica) were assigned to 20 battery brooders, 34 chicks in each. The experimental period lasted 35 days. The treatment groups employed were: 1) a positive control which included 3.5 g available phosphorus (AP)/kg diet and 10 g Ca/kg diet; 2) a negative control which included 2 g AP/kg diet and 8 g Ca/kg diet, 3) negative control diet supplemented with either 300 FTU phytase/kg diet (phytase) or 4) 2.5 g organic acid (lactic acid+formic acid)/kg diet (organic acid); or 5) 300 FTU phytase/kg diet+2.5 g organic acid/kg diet (phytase+organic acid). All birds were fed with the positive control diet for a week and then transferred to the dietary treatments. At the end of the study, there were no differences (p>0.005) among the groups in body weight, weight gain, feed consumption, feed conversion ratio and carcass yield. Tibia ash, however, was reduced (p<0.001) for quails fed the negative control diet containing a low-level of AP compared to the positive control diet containing adequate AP. The addition of phytase, organic acid or phytase+organic acid to the diets containing the low-level of AP improved (p<0.001) tibia ash. On the other hand, an extra synergistic effect of phytase and organic acid on tibia ash was not determined. This study demonstrated that it may be possible to reduce supplemental level of inorganic P with phytase and/or organic acid supplementation for quail diets without adverse effect on performance and tibia ash.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.8
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• pp.1164-1172
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• 2011

Two experiments were conducted to evaluate the efficacy of Trichoderma reesei derived phytase for pigs fed diets with fixed calcium to total phosphorus ratios (1.5:1). In Exp. 1, 280 weaned pigs (initial BW of $10.32{\pm}1.94$ kg) were allocated to one of five dietary treatments on the basis of weight and gender in a randomized complete block design. Treatments were the low phosphorus (0.6% Ca, 0.4% total P and 0.23% available P) diets supplemented with 0, 250, 1,000, or 2,000 FTU phytase/kg of diet and a positive control diet (PC; 0.85% Ca, 0.58% total P and 0.37% available P). The treatments were applied to seven pens with eight pigs per pen, half male and half female. In Exp. 2, six barrows fitted with ileal T-cannula (initial BW = $35.1{\pm}1.6$ kg) were assigned to three dietary treatments with a double $3{\times}3$ Latin square design. The dietary treatments were the low-phosphorus diet (0.53% Ca, 0.34% total P and 0.14% available P), the low phosphorus diet plus 1,000 FTU phytase/kg and a positive control diet (0.77% Ca, 0.50% total P and 0.30% available P). In Exp. 1, there were linear increases (p<0.01) in weight gain, phosphorus absorption, bone strength, calcium and phosphorus content of fat-free dried bone and plasma phosphorus concentrations with increasing dose rate of phytase. The performance of pigs fed the diets with 250, 1,000, or 2,000 FTU of phytase/kg did not differ from pigs fed the PC diet. Pigs fed diets with 1,000 or 2,000 FTU of phytase/kg did not differ from pigs fed the PC diet in bone characteristics. The apparent digestibility of dry matter, crude protein, ash and energy was not affected by dietary treatment. However, pigs fed the PC diet excreted more fecal phosphorus (g/d, p<0.01) and fecal phosphorus per BW gain (g/kg) than pigs fed the diets with phytase. Phytase linearly decreased (p<0.01) fecal phosphorus excreted per BW gain (g/kg), plasma calcium concentration as well as plasma and bone alkaline phosphatase activity. In Exp. 2, phytase supplementation in the low-P diet increased (p<0.05) the apparent ileal digestibility (AID) of Ca, P, leucine, lysine, phenylalanine, alanine and cysteine, tended to AID of crude protein, isoleucine, threonine, asparagine and serine. In conclusion, the novel phytase originated from Trichoderma reesei is effective in releasing Ca, P, and amino acids from corn soy based diet for pigs.

• Korean Journal of Poultry Science
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• v.38 no.4
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• pp.255-263
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• 2011

This work was conducted to investigate the effects of phytase on the growth performance, excreta excretion, and N and P excretion of broilers for 7 weeks. One hundred ninety two Ross broiler (one-day-old) with average weight $44.7{\pm}1.5$ g were assigned randomly to 4 treatments groups (12 birds/pen, 4 pens/treatment) as $2{\times}2$ complex factors of sex of broiler male, female) and phytase 2 treatments (0, 300 FTU/kg), and fed experimental diets for 6 weeks (starter, 0~2 wk; earlier, 2~4 wk; finisher, 4~7 wk). The experimental diets included that phytase was added to the basal diet. Body weight was high at male treatment and at phytase treatment. Feed intake was high at male treatment and was no difference between phytase and non-phytase treatment. Feed efficiency was the highest at male and phytase treatment. Manure excretion was high at male treatment and reduced at phytase treatment. Excretion of N and P was high at male treatment and at non-phytase treatment. Finally, when phyase was added to basal diet, the growth performance of broilers was improved and excreta excretion and N and P excretion of broilers were decreased.

• Korean Journal of Organic Agriculture
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• v.9 no.1
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• pp.51-60
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• 2001

An experiment was carried out to evaluate the effects of adding phytase on nutrient digestibility in growing pigs. Three barrow pigs averaging 28.5$\pm$3.1kg of body weight were allotted to three treatments by 3$\times$3 latin square. Treatments included 1) com-soybean meal based-control diet and 2) and 3) control diet with phytase 500 and 1,000 unit. There were no effects of treatments on dry matter and nitrogen digestibility (P〉0.05). Ash, Ca and P digestibility in pigs fed diet with phytase were greater than those in pigs fed control diet(P〈0.05). An increased phosphorus intake was observed from pigs fed diet with treatments compared to that from pigs fed control diet. Pigs fed diet with adding phytase had improved P absorption compared to pigs fed control diet(P〈0.05). There was no significantly effect on fecal and urine excretion(P〈0.05). Ca intake was increased for pigs fed adding phytase compared to pigs fed control diet. No evident effect was observed on Ca fecal excretion among dietary treatments, however, urine excretion of pigs fed diet with treatments was decreased compared to pigs fed control diet. Cystine, isoleucine, tyrosine and phenylalanine digestibility of amino acid were greater for pigs fed diet with treatments than those for pigs fed control diet(P〈0.05). Asparagine, threonine, serine, methionine and leucine digestibility were improved for pigs fed with phytase 500 unit compared to pigs fed control diet. Lysine, histidine, arginine and other animo acids digestibility were higher for pigs fed diet with phytase than control diet, but there were no significant effects by the treatments(P〉0.05). In conclusion, the results of the experiments suggest that phytase supplementation in the diets could be a very beneficial strategy in environmental aspects.