• Environmental Analysis Health and Toxicology
• /
• v.13 no.3_4
• /
• pp.71-75
• /
• 1998

Carbendazim, which is widely used fungicide, was investigated for rat hepatocarcinogenesis using a medium-term carcinogenicity bioassay. All rats were initially given a single dose (200mg/kg) of diethylnitrosamine (DEN) i.p. and then, starting 2 weeks later, carbendazim treatment group and positive control group received carbendazim (7 mg/kg/ day) and 2-acetylaminofluorene (2-AAF, 1%), respectively, in the diet for 6 weeks. All rats were subjected to two-thirds partial hepatectomy (PH) at week 3 and sacrificed at week 8. Carcinogenic potential was scored by comparing the number and area per cm$^2$ of induced glutathione-S-transferase placental form (GST-P) positive foci in the liver. Carbendazim had no effect in the increase of body weight, hematological and biochemical values, and the number and area of GST-P positive loci. These results suggest that this bioassay using DEN-PH method can be useful for detection of hepatocarcinogenic potentials of pesticide.

• Proceedings of the Ginseng society Conference
• /
• 2002.10a
• /
• pp.47-54
• /
• 2002

Many cultures have traditions that encompass the use of herbs, spices, and other plants for medicinal purposes. From the ancient past to the present medicinal knowledge has been passed down to new generations in the form of shamans, medicine men, healers, and the like. In the past decade there has been a surge of scientific interest in complementary and alternative medicine, much of which has its origins in traditional medicine. It has been recognized for some time that dietary patterns and content affect cancer risk. Epidemiological studies have strongly suggested that the fruit and vegetable content of the diet associates with reduced risk for colon, lung, prostate, and other cancers. Many different types of cellular mechanisms have been postulated by which compounds in botanicals can prevent cancer. Mechanisms particular to the prevention of colon cancer will be addressed in this review.

• Journal of The Korean Society of Inherited Metabolic disease
• /
• v.23 no.2
• /
• pp.15-20
• /
• 2023

Galactosemia is an inborn error disorder of carbohydrate metabolism, caused by metabolic disturbances at various stages of the Leloir pathway. In patients with galactosemia, accurate diagnosis and appropriate care are essential to avoid complications and unnecessary treatments. And a careful differential diagnosis of the type of galactosemia is crucial. Even with an appropriate galactose-restricted diet, long-term complications may occur, especially in patients with classic galactosemia. So new treatment options are being developed. In this review, we will review the new symptoms of each subtype that have been reported recently and GALM (Galactose mutarotase) deficiency, a new form of galactosemia, and treatment policies according to recent guidelines.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.21 no.5
• /
• pp.266-273
• /
• 2020

This study was undertaken to determine the effects of diet supplementation with complex probiotics (CPB), on growth performance, nutrient digestibility, blood metabolites, noxious gas, and fecal microflora in weaning pigs. On the basis of body weight, a total of 234 weaned pigs (Landrace×Yorkshire×Duroc, 6.14±0.78kg) were randomly allotted to 3 treatments and 6 replicates (13 pigs per pen). The experimental diets were fed in a meal form for 28 days (days 0-14, PhaseI, and days 15-28, PhaseII). The dietary treatment groups were as follows: T1 (basal diet), T2 (T1+0.13% CPB) and T3 (T1+0.25% CPB). The CPB supplement contained Bacillus subtilis 1.0×10⁶ CFU/g, Enterococcus faecium 1.0×10⁶ CFU/g, Saccharomyces cerevisiae 1.0×10⁶ CFU/g, Bacillus licheniformis 3.0×10⁸ CFU/g, and Bacillus polyfermenticus 3.0×10⁸ CFU/g. Pigs fed the T3 diet showed an increase (p<0.05) in the overall average daily gain and average daily feed intake, increased (p<0.05) crude protein digestibility in PhaseI, and greater (p<0.05) dry matter and gross energy digestibility in PhaseII. Supplementation of CPB had no effect on the blood profile. Furthermore, pigs fed the T3 diet had lower (p<0.05) NH₃ emission and overall count of fecal Clostridium spp. In conclusion, we believe that CPB supplementation has a beneficial effect on growth performance, nutrient digestibility, noxious gas, and fecal microflora in weaning pigs.

• Journal of Aquaculture
• /
• v.21 no.4
• /
• pp.239-243
• /
• 2008

A $3{\times}2$ factorial experiment was conducted to investigate the proper dietary protein and lipid levels for the growth of sub-adult flounder Paralichthys olivaceus reared during the summer season. Six experimental diets were formulated to contain three levels of protein (45%, 50% and 55%) and two levels of lipid (9% and 14%). Duplicate groups of fish (initial body weight of 298 g) were hand-fed to apparent satiation during the summer season ($21.8{\pm}1.7^{\circ}C$) for 60 days. Survival of each group was over 83% and there was not significant difference among all groups. Weight gain of fish fed the 45% protein diet with 14% lipid was not significantly different from that of fish fed the 50% and 55% protein diets with 9% and 14% lipids, but weight gain of fish fed the 45% protein diet with 9% lipid was significantly lower than that of fish fed the 55% protein diets with 14% lipid. Feed efficiency tended to increase with increasing dietary lipid level at each protein levels, although no significant differences were observed at 50% and 55% protein levels. Protein efficiency ratio, daily feed intake, condition factor and hepatosomatic index were not significantly affected by dietary protein and lipid levels. Crude lipid content of the liver tended to increase with increasing dietary lipid level at the same protein levels, but the opposite appearance was found for moisture content. The contents of moisture, crude protein and crude lipid of the dorsal muscle were not significantly affected by dietary protein and lipid levels. Based on data obtained form this study, inclusion of dietary protein at level of 45% appears sufficient to support optimal growth, and an increase of dietary lipid level from 9% to 14% has beneficial effects on feed utilization of sub-adult flounder during the summer season.

• Toxicological Research
• /
• v.13 no.1_2
• /
• pp.23-27
• /
• 1997

This study was performed to examine the anti-cancer effect of Red Ginseng in the DENGalN-PH-induced hepatic tumor model system in rats. One hundred of male SPF Sprague-Dawley rats(6weeks old) were randomly divided into five groups. Rats in groups 1, 2, 3, and 4 were administered to diethylnitrosamine intraperitoneally 200 mg/kg body weight for the caner initiation. Rats in group 5 were given to saline as a control. On two weeks after cancer initiation, rats in groups 1 and 3 were fed on diet containing 0.01% of acethylaminofiuorene(AAF) which is strong cancer-promotor for 6 weeks, while rats in groups 2 and 4 were fed on water containing 0.05% of phenobarbital which is weak cancer.promotor for 6 weeks. Rats in groups 1 and 2 were treated with diet containing 3% of Red Ginseng for six weeks(from 9th week till 15th week after cancer initiation). Rats in all groups were necropsied time-sequencially at 8, 15, and 36 weeks. The hepatic lesions of rat treated with carcinogens expressed glutathione S-transferase placental form(GST-P) at 8 week. The GST-P positive foci of rats treated with AAF were larger than that of any other rats, while the GST-P positive foci of rats treated with AAF and red ginseng were significantly decreased. This anti-cancer effect of Red ginseng might be involved in the enhacement of natural killer cell activity. To know whether there is direct relationship between Red Ginseng and natural killer cell activity, the activity of natural killer cell was examined after treatment AAF, AAF+Red ginseng and Red ginseng only, respectively. Comparing with natural killer cell activity in AAF-treated group, natural killer cell activity was significantly activated in AAF+ Red ginseng-treated group. This indicated that Red ginseng might enhance natural killer activity after treatment carcinogen in rats. These results suggested that Red ginseng might have a cancer prevention ability by promoting natural killer cell activity during hepatocarclnogenesis.

• Asian-Australasian Journal of Animal Sciences
• /
• v.6 no.3
• /
• pp.377-381
• /
• 1993

Twenty male crossbred ($Sahiwal{\times}HF$) calves of about 6-9 months of age were divided into four groups of five animals each. All the animals were offered wheat straw ad lib. As the basal feed. However, animals in group I were fed concentrate mixture while the animals in groups II, III and IV had free access to urea molasses mineral block (UMMB) lick (I), (II) and (III) respectively as a partial substitute of concentrate mixture. The average concentrate offered (kg/day) to the animals was significantly (p<0.01) higher in group I ($2.69{\pm}0.18$) compared to groups II ($1.76{\pm}0.15$), III ($1.70{\pm}0.06$) and IV ($1.65{\pm}0.12$). The UMMB lick consumed was non-significantly different amongst groups I ($535.40{\pm}38.14$), II ($525.60{\pm}31.82$), III ($551.00{\pm}38.49$) and IV ($548.80{\pm}45.46$). Except ether extract, the digestibility coefficients of CP, ADF and NDF were non-significantly different in different groups. Similarly, N balance (g/day) and percent N retention of intake was not affected in different groups on supplementation of UMMB lick. Body composition of animals was similar in different groups supplemented with either concentrate mixture or concentrate mixture and UMMB licks. It may be concluded from these studies that UMMB lick can partially replace the concentrate mixture in the diet of growing calves without affecting the growth rate, nutrient utilization and body composition. The UMMB lick, thus, can form a part of the ration economically in the diet of growing ruminants especially in developing countries.

• Asian-Australasian Journal of Animal Sciences
• /
• v.16 no.2
• /
• pp.227-233
• /
• 2003

Two experiments were conducted to evaluate the effects of chromium (Cr) on the growth performance, bone trait, serum traits, and immune responses in broilers. The broilers were fed corn-soybean meal basal diet supplemented with Cr at level of 0(control), 200, 400, or 800 ppb in the form of chromium picolinate (CrPic). The broilers were fed treated diets for 6 weeks in Exp. 1, but the Cr supplement was removed for the last 3 weeks in Exp. 2. Exp. 1 showed that dietary supplement of Cr did not affect growth performance of the broiler, though improved feed efficiency (p<0.05) was observed during 0 to 3 weeks. Moreover, serum total (p<0.05) and HDL cholesterols (p<0.06) were significantly higher in pooled Cr added group at 6 weeks of age, however, the difference was not significant in Exp. 2. The pooled Cr added group in Exp.1 had significantly lower (p<0.05) alkaline phosphatase activity and higher (p<0.09) calcium at 3 weeks. Significantly lower phosphorus was also observed in Exp. 2. With continued supplement of Cr as in Exp. 1, the alkaline phosphatase activity maintained higher at 6 weeks, as opposed to significantly lower in Exp. 2, which had no further Cr supplement. Higher bone breaking strength was observed in 400 ppb Cr supplemented in Exp. 1, though not significantly different. Serum glucose and triglyceride were not affected by Cr supplement. Antibody against Infectious Bronchitis (IB) was significantly (p<0.05) higher with 400 ppb Cr supplemented, and anti-Newcastle disease (ND) antibody also tended to be higher (p<0.06) in pooled Cr added group at 6 weeks of age in Exp. 1. Peripheral blood blastogenesis activity was not different among the treatments. The results suggest that diet supplemented with 400 ppb CrPic may be beneficial to the broiler.

• Asian-Australasian Journal of Animal Sciences
• /
• v.30 no.6
• /
• pp.834-842
• /
• 2017

Objective: An experiment was to evaluate the interplay of dietary lipid sources and feeding regime in the transition from sow milk to solid food of abruptly weaned piglets. Methods: Soon after weaning, 144 piglets were selected and were trained over a 15 day period to experience gradually reducing dietary fat content from 12% to 6% for lard (L), soybean oil (S), and coconut oil (C) and their feeding behavior and diet preference then tested in a behavior observation experiment. Another 324 weaned piglets were used in three consecutive feeding experiments to measure the effect of different dietary fats on performance and feed choice in the four weeks after abrupt weaning. The lipid sources were used as supplements in a 3% crude fat corn/soya basal diet, with 6% of each being included to form diets 9C, 9S, and 9L respectively, and their effects on performance measured. Combinations of these diets were then further compared in fixed blends or free choice selection experiments. Results: Piglets pre-trained to experience reducing lipid inclusion showed different subsequent preferences according to lipid source, with a preference for lard at 9%, soybean oil at 3%, and coconut oil at 6% inclusion rate (p<0.001). Following abrupt weaning, whilst after 4 weeks those fed 9C had the heaviest body weights (18.13 kg, p = 0.006). Piglets fed a fixed 1:1 blend of 9C+9S had a poorer feed conversion ratio (FCR = 1.80) than those fed a blend of 9C+9L (FCR = 1.4). The 9C and 9L combination groups showed better performance in both fixed blend and free choice feeding regimes. Conclusion: After abrupt weaning, they still have dependence on high oleic acid lipids as found in sow milk. A feeding regime offering free choice combination of lipids might give the possibility for piglets to cope better with the transition at weaning, but further research is needed.

• Asian-Australasian Journal of Animal Sciences
• /
• v.12 no.1
• /
• pp.42-48
• /
• 1999

Three experiments were conducted to develop and test equations for predicting carcass composition. In the first study using 52 d-old Cobb ${\times}$ Cobb male broilers, twenty four carcasses were selected from 325 processed birds based upon visual appraisal for abdominal fat (low, medium, high) and assayed for specific gravity (SG), dry matter (DM), fat, protein, and ash. In experiment 2, 120 birds were fed rations containing 2 caloric densities (2,880 and $3,200kcal\;ME_n/kg$ diet) and assayed as described above on weeks 2,3,4,5, and 6. Carcass fat was elevated (p < 0.05) with increased caloric density. In both studies predictive variables were significantly correlated with chemically determined carcass fat, protein, and ash contents. Pooled across the 2 studies, data were used to form SG, DM, and or age based equations for predicting carcass composition. Results were tested in experiment 3, where 576 birds reared to 49-d consumed either 2,880, 3,200, or $3,574kcal\;ME_n/kg$ diet while exposed to constant $24^{\circ}C$ or cycling 24 to $35^{\circ}C$ ambient temperatures. Both dietary and environmental effects impacted (p < 0.05) carcass composition. The fat content analyzed chemically was enhanced from 12.4 to 15.7%, and predicted fat was also elevated from 13.4 to 14.8% with increasing caloric density. Heat distress reduced (p < 0.05) analyzed carcass protein (18.9 vs 18.3%) and predicted protein (18.2 vs 17.5%). Predicted equation values for carcass fat, protein, ash, and energy were correlated with the chemically analyzed values at r=0.96, 0.77, 0.86, and 0.79, respectively. Results suggest that prediction equations based on DM and SG may be used to estimate carcass fat, protein, ash, and energy contents of broilers consuming diets that differ in caloric density (2,800 to $3,574kcal\;ME_n/kg$) and for broilers exposed to either constant ($24^{\circ}C$) or cycling high (24 to $35^{\circ}C$) ambient temperatures during 49-d rearing period tested in the present study.