• 한국식품영양과학회지
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• 제43권4호
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• pp.612-617
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• 2014

본 연구에서는 강황 유래의 curcuminoids가 없는 다당체를 이용하여 급성 및 만성 염증 모델에서의 항염증 효과를 검증하고자 하였다. 급성염증에 대한 효과를 알아보기 위하여 carrageenan에 의한 족부종 유발과 xylene에 의한 귀부종 유발을 일으킨 모델을 이용하여 수행하였다. Carrageenan에 의한 족부종 유발 실험에서 강황추출물과 관절염 치료제로 알려진 diclofenac sodium을 처리하였을 때는 부종의 증가도가 낮은 것으로 나타났다($P{\leq}0.05$). 또한 다른 급성염증 모델인 xylene application 마우스 모델에서도 대조군에 비하여 diclofenac sodium과 강황추출물(31.5, 63, 126 mg/kg)을 농도별로 경구투여 하였을 때 염증 억제율이 각각 78.13%, 68.75%, 62.50% 및 59.38%로 나타나 염증 억제율이 높은 것으로 나타났다($P{\leq}0.05$). 만성염증에 대한 효과를 알아보기 위한 cotton pellet 육아종 실험에서는 대조군과 비교하여 삼출물과 염증 육아종이 적게 발생된 것으로 나타났다($P{\leq}0.05$). 따라서 강황 유래의 다당체를 경구투여 하였을 때 만성 및 급성 염증에 대한 저해활성이 있는 것으로 나타나 항염증 소재로서 개발 가능성이 있음을 제시하였다.

• Advances in Traditional Medicine
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• 제7권2호
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• pp.121-127
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• 2007

Curcumin, a dietary pigment responsible for the yellow color of curry, has been used for the treatment of inflammatory diseases and exhibits a variety of pharmacological effects such as anti- inflammatory, anti-tumor, anti-oxidant, and anti-viral activity. In order to examine the potency of the curcumin in inflammation we used carrageenan induced rat hind paw odema model. As curcumin is practically water insoluble, it is hypothesized that pharmacological activity of curcumin could be improved by enhancing its water solubility. Water soluble complexes of curcumin with cyclodextrins were prepared and screened for greater solubility. Pure curcumin 100 mg/kg body weight along with curcumin complexes equivalent to 100 mg/kg body weight of pure curcumin were tested for the anti-inflammatory activity in Wister rats male rats using carrageenan induced hind paw edema model and compared with that of the reference compound diclofenac sodium at a dose level of 10 mg/kg body weight. Results were statistically analyzed using ANOVA. All the treatment groups showed statistically significant anti-inflammatory activity compared with that of vehicle control and positive control.

• Advances in Traditional Medicine
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• 제7권2호
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• pp.191-196
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• 2007

The ethanol extract of leaves of the mangrove Aegiceras corniculatum Blanco (Myrsinaceae) was screened for its antinociceptive, antidiarrhoeal and cytotoxic activities. The extract produced significant writhing inhibition in acetic acid-induced writhing in mice at the oral dose of 250 and 500 mg/kg body weight (P < 0.001), which was comparable to the standard drug diclofenac sodium at the dose of 25 mg/kg of body weight. When tested for its antidiarrhoeal effects on castor oil induced diarrhoea in mice, it increased mean latent period and decreased the frequency of defecation significantly at the oral dose of 500 mg/kg body weight (P<0.05; P<0.01) comparable to the standard drug loperamide at the dose of 50 mg/kg of body weight. Moreover, when tested for toxicity using brine shrimp, the extract showed potent activity against the brine shrimp Artemia salina ($LC_{50}$ 10 mg/ml). The overall results tend to suggest the antinociceptive, antidiarrhoeal and cytotoxic activities of the extract.

• Advances in Traditional Medicine
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• 제6권3호
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• pp.202-207
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• 2006

The crude methanol extract of the roots of Achyranthes aspera Linn. was investigated for its possible antinociceptive, diuretic and neuropharmacological activities in animal models. At the dose of 250 and 500 mg/kg body weight, the extract showed a significant antinociceptive effect in acetic acid induced-writhing in mice comparable to that produced by diclofenac sodium, used as standard drug. The crude extract produced significant diuretic effect at the dose of 500 mg/kg of body weight comparable to that produced by furosemide, used as standard drug. The extract also potentiated significantly the pentobarbital induced sleeping time in mice; decreased the open field score in open field test, decreased the number of hole crossed from one chamber in the hole cross test and decreased the head dip responses. The obtained results provide a support for the use of this plant in traditional medicine and its further investigation.

• Advances in Traditional Medicine
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• 제8권1호
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• pp.24-31
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• 2008

In the present investigation, we studied the effect of alcoholic extract of Moringa oleifera (M. oleifera) seed kernels on various experimental models of bronchial asthma. Significant (P < 0. 05) increase in preconvulsion time was observed due to pretreatment with M. oleifera when the guinea pigs were exposed to either acetylcholine (Ach) or histamine aerosol. This bronchodilating effect of M. oleifera was comparable to ketotifen fumarate. Spasmolytic effect of M. oleifera was also observed by dose dependent inhibition of ideal contractions induced by Ach, 5HT, histamine and $BaCl_2$. Alcoholic extract of M. oleifera produced significant dose dependent protection by egg albumin and compound 48/80 induced mast cell degranulation. Pretreatment with alcoholic extract of M. oleifera also decreased carrageenan induced rat paw edema, which was comparable to that of standard diclofenac sodium. Minimum inhibitory concentration for alcoholic extract of M. oleifera was low as compared to cold-water extract and hot water extract when antimicrobial activity was tested against various respiratory pathogens like Escherichia coli (E. coli), Staphylococus aureus (S. aureus) and pseudomonas aeruginosa (P. aeruginosa). Our data suggest that antiasthmatic activity of M. oleifera seed kernels may be due to its bronchodilator, anti-inflammatory, mast cell stabilization and antimicrobial activity.

• Advances in Traditional Medicine
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• 제6권2호
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• pp.121-125
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• 2006

The crude ethanol extract of the stem bark of Trewia polycarpa (Family: Euphorbiaceae) was subjected to acetic acid induced writhing inhibition, Brine Shrimp lethality bioassay and 1, 1-diphenyl-2-picryl hydrazyl free radical scavenging assay for screening of analgesic, cytotoxic and antioxidant activity respectively. The extract produced significant (P < 0.001) writhing inhibition in acetic acid induced writhing in mice at the dose of 125, 250 and 500 mg/kg body weight respectively, which were comparable to the standard drug diclofenac sodium. The extract showed significant lethality to Brine Shrimp and the $LC_{50}$ value was $8\;{\mu}g/ml$. The extract showed prominent free radical scavenging activity ($IC_{50}$ about ${\sim}10\;{\mu}g/ml$) compare to standard drug ascorbic acid ($IC_{50}about\;{\sim}15\;{\mu}g/ml$). The results tend to suggest that the crude ethanol extract of the bark might possess analgesic, cytotoxic and antioxidant activities or active constituent(s) responsible for the activities.

• The Korean Journal of Pain
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• 제19권2호
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• pp.197-201
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• 2006

Background: There are cases in which shoulder pain persists long after shoulder joint surgery and this pain can not be reduced by intravenous patient controlled analgesia (IVPCA). Our purpose was to evaluate the effect of stellate ganglion block (SGB) on postoperative shoulder pain and also to investigate the effect of preventive SBG on complex regional pain syndrome (CRPS). Methods: Forty patients, who were evaluated to ASA class 1 and 2 and who were scheduled for shoulder joint surgery under general anesthesia, were randomly divided into 2 groups. The experimental group of patients (n = 20) received SGB with 0.5% mepivacaine 8 ml after induction of general anesthesia. The control group of patients (n = 20) received only general anesthesia. Their postoperative pain was assessed using the visual analog scale (VAS) at 30 min, 1, 2, 6, 12, 24 and 48 hours postoperatively. Whenever patients wanted supplemental analgesia, diclofenac sodium 75 mg was injected intramuscularly and the need for supplemental analgesia was recorded. Results: The experimental group of patients had significantly lower pain scores at 30 min, 1, 2 and 6 hours and also significantly lower analgesic requirement at 1, 2 and 6 hours. Conclusions: We found SGB was effective for controlling postoperative pain after shoulder joint surgery. Also, we could expect that SGB reduced the incidence of CRPS.

• 대한의용생체공학회:의공학회지
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• 제36권6호
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• pp.291-295
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• 2015

Various types of implantable drug delivery devices have attracted significant attention for several decades to improve drug bioavailability and reduce side effects, thus enhancing therapeutic efficacy and patients' compliance. However, when implanted into the body, the devices may be influenced by the changes in physiological condition, such as temperature, pH or ionic concentration. Thus, the drug release rates could be also altered concurrently. Therefore, in this work, we employed an implantable ALZET$^{(R)}$ Osmotic Pump, which has been widely used to locally deliver various therapeutic agents and examined the effect of pH, temperature and ionic concentration on its drug release rate. For this, we performed in vitro cell tests to simulate the condition of local tissues influenced by the altered drug release rates, where we used diclofenac sodium as a model drug.

• 한국식품영양과학회지
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• 제43권10호
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• pp.1510-1518
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• 2014

본 연구에서는 구연산칼슘 투여가 papain으로 유도된 골관절염 동물모델에 미치는 영향을 살펴보았다. 8주령의 C57 BL/6J 마우스의 우측 슬관절강에 papain $6{\mu}L$를 투여하여 골관절염을 유발하였다. 실험군은 각 군당 8마리씩 체중이 고르게 배정하여 총 5군으로 나누었다. 정상군은 우측 슬관절강에 생리식염수 $6{\mu}L$를 주사하였고, 대조군, 약물투여군, 구연산칼슘 투여군은 papain $6{\mu}L$로 골관절염을 유발하였다. 정상군과 대조군은 0.2 mL의 생리식염수를, 약물투여군은 diclofenac sodium 2 mg/kg/bw를, 구연산칼슘 투여군은 150, 300 mg/kg/bw를 28일간 1일 매일 정해진 시간에 1회 경구 투여하였다. 실험기간 동안 체중을 측정하였으며, 실험 종료일에는 부검하여 슬관절의 병리조직학적 관찰을 하였고 관절연골 내 proteoglycan 함유율, 골관절염 지수의 변화, 혈액 내 TNF-${\alpha}$, IL-$1{\beta}$, IL-6 함량을 측정하였다. 체중은 실험기간 동안 모든 개체 간의 유의적인 차이는 없었다. 혈액 내 TNF-${\alpha}$, IL-$1{\beta}$, IL-6 함량은 papain 투여 대조군이 정상군에 비해 유의적으로 증가하였으며 약물 및 구연산칼슘 투여군은 대조군보다 유의성 있게 낮았다. 관절연골 내 proteoglycan의 함유율은 약물 및 구연산칼슘 투여군이 papain 투여 대조군보다 유의성 있게 높았다. Hematoxylin과 eosin 염색 관찰 결과, 골관절염이 유발된 마우스에 구연산칼슘을 경구 투여하였더니 관절연골의 파괴와 골 침식 등 연골의 변성이 약물 투여군과 유사하게 나타났다. 이와 같은 결과는 구연산칼슘이 papain으로 유도된 골관절염에서 연골의 변성을 줄이고 염증을 억제함으로써 골관절염 치료에 효과가 있은 것으로 사료되며, 추후 더 자세한 연구가 필요하다.

• 한국식품영양과학회지
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• 제42권8호
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• pp.1183-1189
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• 2013

연구에서는 굴 패각 추출물이 papain으로 유도된 골관절염 동물 모델에 미치는 영향을 살펴보았다. 8주령의 C57BL/6J 마우스 우측 슬관절강에 papain $6{\mu}L$를 투여하여 골관절염을 유발하였다. 실험군은 각 군당 체중이 고르게 8마리씩 배정하여 총 다섯군으로 분류하였다. 정상군은 우측 슬관절강에 생리식염수 $6{\mu}L$ 주사하고 20일간 1일 1회 생리식염수 0.2 mL씩 경구투여 하였다. 대조군, 약물투여군, 굴 패각 추출물 투여군은 골관절염을 유발 후 각각 생리식염수, diclofenac sodium 2 mg/kg/bw, OSE 100, 200 mg/kg/bw를 20일 동안 매일 1회 정해진 시간에 경구투여 하였다. 실험기간 동안 체중을 측정하였으며, 실험 20일에 부검하여 슬관절의 병리조직학적 관찰, 관절연골 내 proteoglycan 함유율, 골관절염 지수의 변화, 혈액내 TNF-${\alpha}$, IL-$1{\beta}$, IL-6 함량을 측정하였다. 체중은 실험기간 동안 모든 개체 간의 유의적 차이가 없었다. 관절연골 내 proteoglycan 함유율은 DS 및 OSE 투여군이 papain 투여 대조군보다 유의성(P<0.05)있게 높았으며, 특히 OSE-200 투여군은 약물 투여군과 유사한 proteoglycan 함유율을 나타냈다. H&E 염색을 통하여 관찰한 결과, 골관절염이 유발된 마우스에 굴 패각추출물을 경구 투여하였을 때 관절연골의 파괴와 골 침식 등 연골의 변성이 약물 처리군과 유사한 것을 보아 굴 패각 추출물이 골관절염 치료에 효과가 있는 것을 확인할 수 있었다. 병리조직학적으로 골관절염 지수는 DS 및 OSE 투여군이 papain 투여 대조군보다 유의성(P<0.05) 있게 낮았으며, OSE-200 투여군이 OSE-100 투여군보다 낮았다. 혈액 내 TNF-${\alpha}$, IL-$1{\beta}$, IL-6 함량은 papain 투여 대조군이 정상군에 비해 유의적(P<0.05)으로 증가하였으며, DS 및 OSE 투여군은 대조군보다 유의성(P<0.05) 있게 낮았다. 특히 TNF-${\alpha}$ 및 IL-6 함량은 OSE-200 투여군이 약물 투여군과 유사한 감소 효과를 나타내었다. 이와 같은 결과는 굴 패각 추출물이 papain으로 유도된 골관절염에서 연골의 변성을 줄이고 염증을 억제함으로써 골관절염 치료에 효과가 있는 것으로 사료된다. 굴 패각은 탄산칼슘이 풍부하기 때문에 골관절염 개선에 효과가 있는 것으로 사료되나, 굴 패각 추출물 중 어떠한 기능성 물질이 골관절염 개선 효과를 나타내었는지에 대해 향후 심도 있는 연구가 필요하다.