• Title/Summary/Keyword: Dichromate

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Protective Effect of Agrimonia pilosa var. Extract on Cultured NIH3T3 Fibroblasts Damaged by Potassium Dichromate (크롬염으로 손상된 배양 NIH3T3 섬유모세포에 대한 짚신나물 추출물의 보호 효과)

  • Lee, Jun-Hee;Seo, Young Mi
    • Korean Journal of Clinical Laboratory Science
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    • v.51 no.2
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    • pp.205-213
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    • 2019
  • The protective effect of Agrimonia pilosa var. (AP) extract on potassium dichromate ($K_2Cr_2O_7$)-induced cytotoxicity in cultured NIH3T3 fibroblasts, was examined by performing an XTT assay for the cell viability and antioxidative effects, such as lactate dehydrogenase (LDH) activity and superoxide anion-radical (SAR) scavenging activity. In this study, $K_2Cr_2O_7$ decreased the cell viability significantly in a dose-dependent manner, and the $XTT_{50}$ value was determined to be $37.5{\mu}M$, which was highly-toxic according to the Borenfreund and Puerner' toxic criteria. The antioxidant, butylated hydroxytoluene (BHT), increased remarkably the cell viability damaged by $K_2Cr_2O_7$-induced cytotoxicity in these cultures. With regard to the protective effect of the AP extract on $K_2Cr_2O_7$-induced cytotoxicity, AP extract produced a significant increase in cell viability and antioxidative effects as the inhibitory ability LDH and SAR scavenging ability. These findings suggest that oxidative stress is involved in the cytotoxicity of $K_2Cr_2O_7$, and the AP extract effectively protected the cells from $K_2Cr_2O_7$-induced cytotoxicity by antioxidative effects. These results suggest that natural resources, such as AP extract, may be a putative therapeutic agent for the diminution or treatment of cytotoxicity induced by heavy metallic bases, such as $K_2Cr_2O_7$ correlated with oxidative stress.

The Effects of Metal Compounds on the Biosynthesis of the Galactolipid and Composition of Fatty Acids in Escherichia coli and Bacillus subtilis (Escherichia coli와 Bacillus subtilis의 당지질 생합성과 지방산 조성에 미치는 금속산화물의 효과)

  • Lee, So Yeon;Yoon, Hyo Sook;Choi, Won Chang;Lee, Chong Sam
    • Journal of Environmental Health Sciences
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    • v.23 no.2
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    • pp.12-23
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    • 1997
  • The biosynthesis of galactolipid, galactose and the fatty acid composition in E. cdi and B. subtills treated with potassium dichromate(PD, 500 ppm, 500 ppm), potassium chromate(PC, 500 ppm, 500 ppm), cobalt chloride(CC, 100 ppm, 10 ppm) and methylmercuric chloride(MC, 100 ppm, 10 ppm) during the culture were analyzed to compare with the control. The growth rate of cells, the contents of monogalactosyldiglyceride(MGDG), digalactosyldiglyceride(DGDG) and total lipid in the metal compound treatments were lower as compared with the control. And too, the contents of galactose utilized for the biosynthesis of galactolipids in these strains in the various metal compounds treatments were inhibited. The fatty acids used for the MGDG and DGDG formation in E. coli and B. subtills treated with each metal compounds during the culture were showed to the variant compositional change.

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Phytoplankton as Standard Test Species for Marine Ecotoxicological Evaluation (해양생태독성평가를 위한 표준시험생물로서의 식물플랑크톤에 관한 연구)

  • Park Gyung-Soo;Lee Sang-Hee;Lee Seung-Min
    • Journal of Environmental Science International
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    • v.14 no.12
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    • pp.1129-1139
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    • 2005
  • A series of experiments was conducted to identify the potential of five phytoplankton species as standard test species for marine ecotoxicological tests. The candidate phytoplankton species are Skeletonema costatum, Heterosigma akashiwo, Prorocentrum micans, Isochrysis galbana, and Tetraselmis suecica. Salinity tolerance and sensitivity on potassium dichromate as a reference material were identified. Toxicity of eleven ocean dumped sewage sludges and four red tide expellent extracts were estimated by the inhibition of population growth rates (PGR) of marine diatom S. costatum, While most species revealed relatively weak tolerance on salinity, T. suecica demonstrated the highest salinity tolerance ranged from $5\~35$ psu and the others $15\~35$ psu. H. akashiwo revealed the highest sensitivity as 72h $IC_{50}$=0.76mg/L and T. suecica the lowest as 72h $IC_{50}$=8.89mg/L on potassium dichromate. Sludge extracts from industrial waste, domestic sewage and livestock farm waste sludge showed high toxicity as 72h $IC_{50}$<$2\%$ and lowest toxicity from filtration bed sludge as 72h $IC_{50}$=$30.50\%$ NOEC (No Observed Effective Concentration) of sludge extract ranged from <$0.4\%$ to $1.6\%$ and this indicated high phytotoxicity of ocean dumped sewage sludge. The test sensitivity of phytoplankton PGR inhibition was much higher than those of marine rotifer Brachionus plicatilis mortality test and bioluminescent inhibition test by marine bacteria Vibrio fischeri, and comparable with the sea urchin (Strongylocentrotus intermedius) fertilization test. As a result the phytotoxicity test using phytoplankton PGR inhibition ($IC_{50}$) must be a useful tool for marine phyto-toxicological evaluation of ocean dumped materials.

Analysis of Inorganic Acids by Capillary Zone Electrophoresis (Capillary Zone Electrophoresis를 이용한 음이온 동시분석에 관한 연구)

  • Park, Sung-Woo;Jin, Kwang-Ho;You, Jae-Hoon;Kim, Dong-Hwan;Seo, Baeseuk;Kim, Young-Sang
    • Analytical Science and Technology
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    • v.11 no.3
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    • pp.213-221
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    • 1998
  • The determination of inorganic anions by capillary zone electrophoresis is reported. A ten component synthetic mixture of anions of bromide, chloride, fluoride, nitrite, nitrate, sulfite, sulfate, perchlorate, chlorate and chlorite was separated by the capillary column and detected by indirect UV method. The running buffer contained 5 mM ammonium dichromate, 10 mM ammonium acetate, 20 mM diethylenetriamine, 10% methanol solution at pH 9.3. A potential of 15 kV at the cathode (reversed polarity) was utilized for the separation of inorganic anions. A complete separation of anions was achieved in less then 10 min and the applicabilities of the method for the analysis of real samples was demonstrated. We compare the concentration of anions in toluene inhaled humen's urine and in postmortem bloods obtained by capillary zone electrophoresis and ion chromatograph.

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Surface Modification of Low Density Polyethylene and Adhesion Characteristics of Low Density Polyethylene/Aluminum Laminate (저밀도 폴리에틸렌의 표면개질과 이를 이용한 저밀도 폴리에틸렌/알루미늄 라미네이트의 접착특성)

  • Jung, B.Y.;Ryu, S.H.
    • Elastomers and Composites
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    • v.36 no.3
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    • pp.195-200
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    • 2001
  • Ultraviolet photografting of acrylic acid onto low density polyethylene was characterized using XPS and contact angle measurement. Effects of surface modification at LDPE and aluminum on LDPE/Al laminate were also investigated. Contact angle decreased significantly at initial state arid tends to level off with increasing UV irradiation time. The improvement of hydrophilicity was due to the presence of acrylic acid on LDPE surface. Graft of acrylic acid onto LDPE was also identified from O1s/C1s ratios in XPS spectrum. Adhesion strength of LDPE-g-AAc/Al laminate showed about 30 times higher than LDPE/A1 system and it could be attributed to the increase of polarity of LDPE surface. Chemical treatment of Al surface using sulfuric acid/sodium dichromate also increased the adhesion strength of LDPE/Al laminate. Adhesion strength of LDPE/Al laminate decreased significantly under acetic acid.

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A Study on High Efficiency of Phase Grating (은염에 형성된 위상형 회절격자의 고효율에 관한 연구)

  • Kim, Nam;Gil, Sang-Keun;Park, Han-Kyu
    • Journal of the Korean Institute of Telematics and Electronics
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    • v.26 no.3
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    • pp.145-152
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    • 1989
  • Volume phase diffraction grating is formed in 5${mu}m$ Agfa 8E75 photographic emulsion film using He-Ne laser. It is dependent on the chemical processing rather than materials themselves that high diffraction efficiency can obtained in silver halide emulsions. With potassium dichromate bleach and alcohol drying, overall efficiency of 71% (81% after allowing for reflection at the two surfaces) has been achieved at an exposure of 180${mu}J/cm^2.$ Dichromate cross-linkage and air void by rapid dehydration increase refractive index modulation depth. The grating is swollen, which is newly identified by Scanning Electronic Microscope(SEM) photography, and it causes on-Bragg at larger angle than the recording angle. It is pointed out that controlling the emulsion thickness has an important role as a potential source for high diffraction efficiency.

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An Efficient Method for Selective Deprotection of Trimethylsilyl Ethers and Tetrahydropyranyl Ethers under Solvent-free Conditions

  • Hajipour, A.R.;Bagheri, Hamid R.;Ruoho, Arnold E.
    • Bulletin of the Korean Chemical Society
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    • v.26 no.11
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    • pp.1689-1691
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    • 2005
  • 1-Butyl-4-aza-1-azoniabicyclo[2.2.2]octane dichromate (BAABOD) 3 is a useful reagent for the selective cleavage of trimethylsilyl ethers and tetrahydropyranyl ethers to their corresponding aldehydes and ketones in the presence of $AlCl_3$ under solvent-free conditions. This method is very simple and efficient and the reaction has been carried out under solvent-free conditions in the presence of a catalytically amount of aluminum chloride.

A Study on the Current Efficiencies in the Electrolytic Preparation of Sodium Chlorate by Lead Dioxide Anode (二酸化鉛 陽極에 의한 鹽素酸나트륨 電解製造에 있어서 電解條件 및 電流效率에 관한 硏究)

  • Nam Chong Woo
    • Journal of the Korean Chemical Society
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    • v.13 no.2
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    • pp.165-169
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    • 1969
  • On the electrolytic preparation of sodium chlorate, lead dioxide anode, instead of graphite was tested to find out the characteristics for current efficiency and life in various conditions. The results obtained are summerized as follows; 1. The current efficiency is slightly increased with the anode current density, until 25A/$dm^2$ 2. The higher the current concentrations. the lower current efficiencies are observed, particularly in case of both not-adding the potassium dichromate and large current concentration of more than 50A/l 3. The current efficiency may be improved linearly as the both temperature is raised.

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Studies on the Dyeing of Hanji by Natural Dye-stuffs(II) -With a focus on the Onion-peelings- (천연염료를 이용한 한지염색에 관한 연구(II) -양파껍질을 중심으로-)

  • Jeon, Chul
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.35 no.1
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    • pp.48-53
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    • 2003
  • The objective of this study was to find in what color Korean handmade paper(Hanji) is dyed when it is dyed with a pigment extracted from waste onion peelings using different kinds of mordant, and how the paper is discolored and variety of strength under the condition of accelerated aging test. The results of this experiment are as follows. 1. Korean handmade paper was dyed in different colors according to the kinds of mordant. Mainly it was dyed in orange-brown, and sometimes in gold or khaki. 2. Korean handmade paper dyed was not discolored much, which suggests that the pigment is strong under the condition of aging. 3. As for durability, Korean handmade paper dyed in gold was strongest. Korean handmade paper, to which aluminum sulfate, iron sulfate or sodium dichromate was applied as mordant, had poor durability. Thus, these were not suitable as mordant.