• Mycobiology
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• 제38권2호
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• pp.156-158
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• 2010

Stem rot disease was found in garlic (Allium sativum L.) cultivated from 2008 to 2010 in the vegetable gardens of some farmers in Geumsan-myon, Jinju City, Gyeongnam province in Korea. The initial symptoms of the disease were typical water-soaked spots, which progressed to rotting, wilting, blighting, and eventually death. White mycelial mats had spread over the lesions near the soil line, and sclerotia had formed over the mycelial mats on the stem. The sclerotia were globoid in shape, 1~3 mm in size, and tan to brown in color. The optimum temperature for growth and sclerotia formation on potato dextrose agar (PDA) medium was $30^{\circ}C$. The diameter of the hyphae ranged from approximately 4 to $8\;{\mu}m$. Typical clamp connection structures were observed in the hyphae of the fungus, which was grown on PDA medium for 4 days. On the basis of the mycological characteristics and pathogenicity of the fungus on the host plants, the causal agent was identified as Sclerotium rolfsii Saccardo. This is the first report of stem rot disease in garlic caused by S. rolfsii in Korea.

• Mycobiology
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• 제38권2호
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• pp.118-121
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• 2010

Single ascospore isolates of Cordyceps bassiana were observed for their colony pigmentation on Sabouraud Dextrose agar plus Yeast Extract (SDAY) plates and were inoculated in a brown rice medium for production of fruiting bodies. Colony pigmentation did not show any relationship with perithecial stromata formation. The isolates were also grown on opposite sides of SDAY agar plates and were observed for vegetative compatibility. Neither vegetative compatibility nor perithecial stromata could be found to be related to each other. It was concluded that fertile fruiting body production was independent of colony pigmentation and vegetative compatibility. Synnemata formation was found to be more common than perithecial stromata formation. This might be due to its highly conidiogenous anamorphic stage, i.e., Beauveria bassiana.

• 한국균학회지
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• 제46권1호
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• pp.28-33
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• 2018

A fungal isolate designated KNU16-033 was isolated from a soil sample in Daegu, Korea. White, short, and felt-like aerial mycelia appeared on the surface of colonies of the isolate. Colonies with a smooth texture developed slowly and reached a diameter of 78 mm after 21 days of incubation on potato dextrose agar. This isolate displayed globose, colorless or light yellow sporangiospores, which differed morphologically from the sporangiospores of Gongronella butleri, which were hyaline, oval to flattened on one side or almost reniform. Based on these morphological characteristics and phylogenetic analysis using internal transcribed spacer regions, the isolated fungus was identified as G. guangdongensis belonging to Cunninghamellaceae. To our knowledge, this is the first record of G. guangdongensis in Korea.

• 한방안이비인후피부과학회지
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• 제13권1호
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• pp.267-279
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• 2000

Minimum inhibitory concentration(MIC) of Sophorae Subprostratae Radix. Aconiti Radix, Hibisci Syriaci Cortex and those mixture. Phellodendri Cortex, Sophorae Radix, Torilis Fructuson on various species of the dermatophytes on Microsporium gypseum, Trichophytone rubrum and Epidermophytone floccosum were determined after 4 weeks of incubation at $25^{\circ}C$ in Sabouraud dextrose broth. The results were summarized as followes : 1. Antifungal effects of Phellodendri $Cortex\;{\cdot}Hibisci$ Syriaci Cortex and mixture of Sophorae Subprostratae Radix, Aconiti Radix, Hibisci Syriaci Cortex on T rubrum which is the most common organism of tinea pedis were more effective than that of the others. 2 These results suggested that herbal medicines such as Phellodendri $Cortex\;{\cdot}Hibisci$ Syriaci Cortex and mixture of Sophorae Subprostratae Radix, Aconiti Radix, Hibisci Syriaci Cortexse can be used to treat tinea pedis and to improve untoward effects associated with the administration of antifugual agents.

• 한국미생물·생명공학회지
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• 제25권3호
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• pp.305-310
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• 1997

For the continuous production of transglucosylated steviosides, cyclodextrin glucanotransferase from Bacillus macerans was immobilized onto Diaion HPA 75 (styrene-divinylbenzene resin) that was screened from ion exchange resins, synthetic adsorbents and chitosan derivatives. The parameters influencing enzyme immobilization were examined in order to maximize the activity of immobilized enzyme. The optimum conditions for immobilization turned out to be: contact time 2 hr at 30$circ$C, pH 6$sim$9, and enzyme loading 20mg protein/g resin at 4.4 Os/Kg as osmolarity. Competing with other molecules having low molecular weight, enzyme was immobilized reversibly. The activity of immobilized enzyme was as high as 180U/g resin when the diafiltrated solution of stock enzyme was used. The optimum conditions for transglucosylation were as follows: pH 6.0, temperature 50$circ$C, 30% substrate solution composed of 15% stevioside mixture and 15% dextrin of which value of dextrose equivalent was about 9.0.

• 한국균학회지
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• 제42권4호
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• pp.349-352
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• 2014

The fungal endophyte EML-CM25 was isolated in Korea from surface sterilized Chinese maple leaf tissues. Internal transcribed spacer sequence analysis indicated that the isolate showed 99% sequence similarity with Paraconiothyrium brasiliense, a finding that was confirmed by morphological analyses. The fungal colonies did not express aerial hyphae. Conidiomata formation was observed in the fungus cultured on potato dextrose agar at $25^{\circ}C$ for 7 days. Visible scattered black dots consisting of pycnidia were present throughout the colony. This is the first record of P. brasiliense in Korea.

• 한국임상수의학회지
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• 제10권1호
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• pp.137-140
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• 1993

An English bulldog, three years old, hospitalzed as vomitting, severe abdominal pain and intermittent body tremor. In blood examination, WBC, amylase, alkaline phosphatase value showed higher than normal value, so we diagnosed as acute pancreatitis. The bulldog was treated with fluid therapy as Ringer's solution, saline and 5% dextrose, and antibiotics as mixed penicillin with streptomycin, and cephazolin. To prevent shock, dexamethasone was medicated in early time. Also the bulldog was medicated as banamine, vitamine K, atropine and cimetidine. When the English bulldog showed improvement we gave him hill's i/d continuously. Through these procedure, the English bulldog recovered completely.

• Mycobiology
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• 제36권3호
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• pp.152-156
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• 2008

The fungus Phellinus is a mushroom that is widely used medicinally. The optimal conditions for mycelial growth of 13 strains of the fungus were investigated. Mycelial growth was optimal at 25$^{\circ}C$ and was uniformly minimal at 15$^{\circ}C$ and 35$^{\circ}C$. Growth was optimal at pH 6$\sim$7. The mycelial phenotype was best promoted by growth using Potato Dextrose agar, Hamada, Glucose peptone, and Yeast-Malt media, whereas Czapek Dox, Hennerberg, and Lilly media were the most unfavorable for the mycelial growth of Phellinus spp. Glucose, sucrose, fructose, and dextrin were the most suitable carbon sources for mycelial growth, while lactose, maltose, and galactose were unsuitable. Among tested nitrogen sources, ammonium phosphate, potassium nitrate, and arginine best promoted mycelial growth, while alanine, urea, and histidine least promoted mycelial growth.

• The Plant Pathology Journal
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• 제15권5호
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• pp.308-310
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• 1999

A fungus associated with gray blight on tea plant (Camellia sinensis) was identifed as Pestalotiopsis theae based on the mycological characteristics. Mycelial growth on potato dextrose agar and size and shape of conidia of P. theae were similar to those of P. longiseta, but P. theae was different from P. longiseta in the color of three median cells and the number of apical appendages. Artificial inoculation of conidial suspension or mycelial mats on the wounded leaves and shoots of healthy plants induced the same disease, respectively. The Korean native variety was relatively. The Korean native variety was relatively more resistat to P. theae than a Japanese variety‘Yabukita’which has bee recently introduced and planted in large areas of southern parts of Korea. Here, we report the report the first record of gary blight caused by P. theae on tea plant in Korea.

• 한국임상약학회지
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• 제10권2호
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• pp.74-79
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• 2000

Y-Site 투여시 $5\%$포도당액과 생리식염수에서 탁솔과 은단세트론의 안정성에 관해 실온과 형광등 아래서 연구하였다. 온단세트론 0.03 mg/ml, 0.1 mg/ml, 0.3 mg/ml와 탁솔 0.3 mg/ml, 1.2mg/ml를 각각 1:1로 혼합한 후 0, 1, 2, 4, 12시간에서 즉시 약물농도를 HPLC로 분석하였다. 방해물질에 의한 분석오차를 줄이기 위해 분석법을 여러상태에서 확인하였으며, 각 농도에서 3차례씩 실험하였고 각 샘플은 2차례 연속 HPLC 분석하였다. 분석전에 각 시료의 투명도, 색의변화, 침전상태 및 pH를 검사하였다. 온단세트론 0.03, 0.1 및 0.3 mg/ml와 탁솔 0.3 및 1.2mg/ml를 각각 혼합하였을 때 12시간 동안 안정성이 있었다. 혼탁이나 색의 변화 및 침전은 나타나지 않았으며 12시간 동안 pH의 변화는 특별한 경향이 없었다.