• 제목/요약/키워드: Dextrose

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Morphological Variations in Conidia of Arthrobotrys oligospora on Different Media

  • Singh, R.K.;Kumar, Niranjan;Singh, K.P.
    • Mycobiology
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    • 제33권2호
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    • pp.118-120
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    • 2005
  • Most commonly occurring predacious fungus Arthrobotrys oligospora showed great variation in size and shape of conidia on some media. The formation of larger conidia was recorded on beef extract and nutrient agar media. The length of conidia in Richard's YPSS, Sabouraud's, PDA and com meal agar media was of medium size while smaller conidia were produced on Czapek's, Jensen's, Martin's medium. Maximum width of conidia was recorded on YPSS medium followed by Sabouraud's medium. The average size of spores on nematode infested com meal agar medium was slightly increased than those on com meal agar medium.

Molecular Cloning and Expression of Genes Related to Antifungal Activities from Enterobacter sp. B54 Antagonistic to Phytophthora capsici

  • YOON, SANG-HONG
    • Journal of Microbiology and Biotechnology
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    • 제9권3호
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    • pp.352-357
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    • 1999
  • Enterobacter sp. B54 inhibited growth of the fungus Phytophthora capsici on potato dextrose agar (PDA). Three mutants with antifungal activities (denoted M54-47, M54-113, and M54-329) which were lost or increased, through Pl::Tn5 lac mutagenesis, were used to isolate genes responsible for fungal inhibition on PDA. Two clones were selected from the partially EcoR1-digested genomic library of the wild-type strain by probing with genomic flanking sequences of each mutant. We have isolated a 20-kb EcoR1 genomic DNA fragment from this strain that contains genes involved in hyphal growth inhibition of P. capsici on PDA. Subcloning and expression analysis of the above DNA fragment identified a 8-kb region which was necessary for antifungal activities. A 8-kb HindⅢDNA fragment covers three genomic loci inserted by Tn5 lac in each mutant. This suggested that all genes which are related to antifungal activities might be clustered in simple forms of at least 5-8 kb sizes.

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콩, 팥 및 녹두에서 분리한 탄저병균류의 동정과 병원학적 특징 (Identification and Etiological characteristics of Anthracnose Fungi Isolated from Soybean, Small Red Bean and Green Bean)

  • 한경숙;이두형
    • 한국식물병리학회지
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    • 제11권1호
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    • pp.30-38
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    • 1995
  • Anthracnose fungi associated with soybean (Glycine max), small red bean (Vigna angularis), and green bean (Phaseolus radiatus var. aurea) were compared with respect to cultural characteristics, morphological aspects, and pathogenicity. From the legumes, four different species of Colletotrichum were identified based on their morphological characters : C. truncatum, C. destructivum, C. trifolii and C. gloeosporioides. Colonies of Colletotrichum spp.on culture media varied in colour and growth habits depending upon the media and isolates. In general, formation of conidia, setae, and perithecia on water agar leaf medium was better than that on potato dextrose agar medium under near ultraviolet light. In cross inoculation tests, isolates of C. truncatum, c. destructivum, C. trifolii and C. gloeosporioides could infect soybean, small red bean, and green bean at different growth stages. There were some differences in pathogenicity or susceptibility depending on crops tested. C. truncatum was the most virulent and major pathogenic species among the four species.

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Isolation, Cultivation, and Antifungal Activity of a Lichen-Forming Fungus

  • Hur, Jae-Seoun;Kim, Hye-Jin;Lim, Kwang-Mi;Koh, Young-Jin
    • The Plant Pathology Journal
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    • 제19권2호
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    • pp.75-78
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    • 2003
  • A lichen-forming fungus was successfully isolated by discharged spore method from Korean lichen (Hetero-dermia sp.) and cultivated in pure culture. The isolate JR0012 inhibited mycelial growth of several plant-pathogenic fungi. Mycelial growth of the four Pythium spp. tested was completely inhibited. Potato dextrose broth was found to be the medium favorable for large-scale production of antibiotics from the isolate. Anti-fungal substances produced in axenic culture were partially purified. This is the first report in Korea of lichen-forming fungus successfully isolated and which exhibited strong antifungal activity against plant-pathogenic fungi, especially the four Pythium spp..

Degenerative joint disease with systemic mycoses in a oriental white stork

  • Lee, Sook-Jin;Han, Je-Ik;Chang, Dong-Woo;Na, Ki-Jeong
    • 대한수의학회지
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    • 제47권1호
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    • pp.95-98
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    • 2007
  • Rehabilitation Research was presented to Veterinary Medical Center of Chungbuk National Universitywith anorexia and lameness for 5 days. Bilateral intertarsal joint swellings were observed in physicalexamination. The radiographic findings indicated degenerative changes of joint cartilage and suroundingbones. In cytologic examination of synovial fluids, mononuclear leukocytic inflammation was identified.on Sabroud dextrose agar. From all of examinations, this patient was diagnosed as a degenerative jointdisease with systemic mycoses.

수종의 식물생장촉진 근권미생물의 분리 및 근권처리가 오이와 토마토 플러그묘의 초기생장에 미치는 영향 (Selection and Bacterialization into Rootzone of the Various Plant Growth Promoting Rhizobacteria in Peatmoss Compost on the Early Growth of Cucumber and Tomato Plug Seedlings)

  • 조자용;김광수;정순주
    • 한국유기농업학회지
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    • 제6권1호
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    • pp.151-160
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    • 1997
  • Azospirillum sp., photosynthetic bacteria(Rhodopseudomonas sp.) and Pseudomonas sp. were separated and screened from soil and soilless culture, and identificated. The antifungal activities against root-infected pathogens and plant growth promoting effects of the cultured solution of the starins(5.0$\times$105 cells/$m\ell$) in the peatmoss compost on the early growth of cucumber and tomato seedling were investigated. Azospirillum sp. and Pseudomonas sp. showed a antifungal activities against Fusarium sp., Pythium sp. and Rhizoctonia sp in thed ranges of 51.0% to 72.0% on potato dextrose agar medium, however photosynthetic bacteria had not antifungal activities. When cultured solution of Azospirillum sp., photosynthetic bacterial and Pseudomonas sp. were bacterialized by mixing with peatmoss compost, early growth of cucumber and tomato in terms of plant height, number of leaves, leaf area, root length, fresh anf dry weight of leaf, stem and root were promoted, especially photosynthetic bacteria had a the best plant growth promting activities.

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고추 역병균(Phytophthora capsici)의 발육과 감염에 미치는 산성전해수의 영향 (Effect of Acidic Electrolyte Water on Growth and Infection of Phytophthora capsici)

  • 이중환;권태룡;문재덕;이준탁
    • 한국식물병리학회지
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    • 제14권5호
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    • pp.440-444
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    • 1998
  • This experiment was carried out to elucidate the effect of electrolytic water on the growth and infection of Phytophthora capsici. Zoospores of P. capsici did not grow on potato dextrose agar when the pathogen was cultured after suspended in electrolytifc water (pH 2.5, 3.0, 3,5) with HCI solution. When the 100 ml of electrolytic water (pH 2.5, 3.0, 3.5) was irrigated on the red pepper plants that had been inoculated by P. capsici (103 zoospores/ml), the red pepper plants were not infected but irrigated with sterilized water (pH 6.5) the red pepper plants were infected. With this result, it could be concluded that the good sterilization effect on P. capsici might be obtained by applying electrolytic water.

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호흡기 질환 환자로부터 분리한 Scophulariopsis brevicaulis에 관한 연구 (Studies on the Scophulariopsis brevicaulis Isolated from the Respiratory Disease Patients)

  • 렴곤;유승조;인상환;이장훈
    • 환경위생공학
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    • 제3권1호
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    • pp.1-8
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    • 1988
  • It has been reported that the pulmonary mycosis generally results from saprophytic colonization of pre-existing lung cavities usually due to pulmonary tuberculosis etc. Culture of homogenized tissues yield pure colony of slightly brown on the Sabouraud dextrose medium. The fungus was identified as the Scophulariopsis brevieaulis which had been rarely reported in pulmonary fungal infection. Histopathological examination of excised tissues revealed a chronic granulomatous inflammation and many branched hyphal mass. Susceptibility tests were tested with ketoconazole, griseofulvin, nystatin, and amphotericin B. Of these antifungal agents, ketoconazole and griseofulvin proved to be most effective and minimum inhibitory concentration was $10{\mu}g/ml,\;50 {\mu}g/ml$, respectively.

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큰재갈매기에서 발생한 폐 아스퍼질러스증 1예 (Pulmonary aspergillosis in a slaty-backed gull ( Larus schistisagus ))

  • 윤정식;강상철;윤영민;이상;김재훈
    • 한국동물위생학회지
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    • 제35권1호
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    • pp.73-76
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    • 2012
  • A female slaty-backed gull (Larus schistisagus) died suddenly without apparent clinical signs. At necropsy, well demarcated 7 to 10 mm yellow to white nodules were presented at the lungs and thoracic cavity. Microscopically, multifocal necrotic granulomas were observed in the lung tissue and amorphous acidophilic fibrin were accumulated in the granuloma and normal alveolar space. Periodic acid-Schiff (PAS) staining demonstrated numerous pinkish red branched hyphae embedded in the center of these granulomas. According to fungal culture using Sabouraud's dextrose agar plate, Aspergillus fumigatus was isolated from lung lesions. This is the first report for pulmonary aspergillosis of wild slaty-backed gull in Korea.

Identification of Aspergillus nidulans from cooked eggs produced by permitted factory

  • Park, Hye-Won;Kim, Kook-Joo;Youk, Ji-Hea;Woo, Jong-Tae
    • 한국동물위생학회지
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    • 제30권2호
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    • pp.283-286
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    • 2007
  • Fungus generally doesn't produce toxic or harmful substances so it has low chances to cause food poisoning. However it leads to change appearance, odor and characteristics of the contaminated foods and result in sanitary risk problems. Therefore the contamination of fungi should be prevented since they are not proper for human consumption. Green fungi with white outline raised from the air cell of cooked eggs which were collected by Gyeongi Livestock Veterinary Service in August, 2006. The results came out after the cultivation using Sabouraud's Dextrose Agar(SDA). The conidium appeared white and monospore, the shape of colony was round and oval. Conidiophore was brown and granulated and wrinkles and formed. It was confirmed as Aspergillus nidulans based on the dying using Lactophenol cotton blue, the observation of septum and vesicle from the grown spores, and rDNA sequencing.