• Reproductive and Developmental Biology
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• 제31권2호
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• pp.109-113
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• 2007

This study was conducted to evaluate the effect of cytochalasin B (CB) treatment in the activation medium on the development of somatic cell nuclear transfer (SCNT) rat embryos. Fetal fibroblast cells were isolated from a Day 14.5 fetus, and the oocytes for recipient cytoplasm were recovered from 4-week old Sprague Dawley rats. After enucleation and nuclear injection, the reconstructed oocytes were immediately exposed to activation medium consisting of 10 mM $SrCl_2$ with or without CB for 4 hr, and formation of pseudo-pronucleus (PPN) was checked at 18 hr after activation. Then, they were transferred into day 1 pseudopregnant recipients (Hooded Wistar) or cultured for 5 days to check their developmental competence in vivo or in vitro. The number of PPN was not affected by CB treatment during the activation. However, CB treatment supported pre-implantation development of rat SCNT embryos. Embryos generated by the procedures of SCNT were also capable of implanting, with 1 implantation scar found from a recipient following the transfer of 87 SCNT embryos to four foster mothers. The result of the present study shows that rat SCNT embryo can develop to post-implantation stage following treatment with CB.

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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• pp.69-69
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• 2003

Neuroprotective strategies have been appeared to be effective in a variety of stroke models. One of the major focuses has been related to the activities of estrogen. $17\beta$-estradiol valerate(EV) has been reported to exert neuroprotective effects when administered before an ischemic insult. The purpose of this study was to determine whether EV can protect against brain injury via estrogen receptor. Chronic and acute pretreatment can reduce the ischemic damage of focal cerebral ischemia in OVX rat, indicating that EV may be a new therapeutic class of drugs to prevent neuronal damage associated with cerebral ischemia. RNAs were extracted from the hippocampus of ovariectomized female rat with or without EV. Differential gene expression profiles were revealed(Bone morphogenetic protein type 1A receptor, Protein disulphide isomerase, cytochrome bc-1 complex core P, thiol-specific antioxidant protein). RT-PCR and in situ hybridization were used to validate the relative expression pattern obtained by the cDNA array. This Study was supported by the Korea Science and Engineering Foundation(KOSEF) through the Biohealth Products Research Center(BPRC), Inje University, Korea

• 대한가정학회지
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• 제36권11호
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• pp.119-128
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• 1998

This study was designed to find tendencies of housing value orientation of the model steel house visitors. Questionnaires were collected in June, 1997, 1,550 in total, 508 cases from Seoul, 456 cases from Pohang, and 486 cases from Kwangyang. Data were analyzed in terms of housing value orientation, using statistical methods including frequencies, mean, percentages, chi-square test and multiple regression. The major findings are as follows; 1. The subjects were highly educated(64% of them were college graduates) with the monthly income over 2,000,000won(approximately $1,540). The average monthy income of the subjects in Seoul, about 4,000,000won($3,070), was much higher than that of other areas. Most of the subjects were condominium owners(72%), who preferred single detatched houses or town houses located in suburban areas. 2. Differences in general tendencies among the three regional groups were statistically significant. Most significantly, subjects living in Pohang regarded the reputation of the building construction company most importnat. Subjects of all three regional areas, especially in Pohang, emphasized investment value. The price of the steel house was regarded as very important by all the groups tested. The housing value orientation was analyzed in 3 aspects; (1) Facility.Plumbing.Structure(FPS), (2) Interior space formation.Design(ID) and (3) Developmental complex.Near Environment(DN). Interior environment level, stability of house structure, and finighing state of interiors were identified as the important factors in the area of FPS(p<.05). The Interior plan organization was the most important factor among ID. As for DN, rated importantly were educational facilities, green areas, convenience of commercial facilities within the developmental complex were rated importantly.

• 한국정보교육학회:학술대회논문집
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• 한국정보교육학회 2010년도 하계학술대회
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• pp.151-160
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• 2010

학습순행 (學習順行: Learning Progressions)은 학습자 이해의 발달 단계를 나타내기 위해 유용한 방법이다. 본 고에서는 초임 교사가 익혀야하는 교수 지식을 체계화하고 교사교육 프로그램 개발을 발달적 관점에서 접근하기 위한 방법으로 학습순행을 소개한다. 학습순행의 개발과 타당화 과정을 제안하고, 이와 관련하여 한 과학 교사 연수 프로그램에서의 개발된 과학탐구 수업에서 ICT 통합을 위한 학습순행과 그 개발과정을 논의한다. 초임교사 전문성 향상을 위한 학습순행의 이용은 교사의 이해 정도를 평가하기 위한 근거를 제공하며, 체계적이고 발달적인 관점에서 교사 연수 프로그램을 개발할 수 있도록 지원하는 등의 다양한 장점이 있다.

• 한국정보통신학회논문지
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• 제3권4호
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• pp.695-704
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• 1999

세계 각국들이 수산자원 확보를 위해 경제수역을 선포하고 있으며, 우리나라의 경우 이미 선포된 한ㆍ일 어업 협정에서의 협상문제, 앞으로 진행될 한ㆍ중 어업 협상의 난항 등 여러 가지 문제로 수산정책의 변화가 전망된다. 연구는 새로운 수산정책을 모색하기 위하여 현재의 문제점을 체계적으로 분석하고 미래에 적합한 수산정책을 제시하였다. 수산정책의 발전방향은 해양수산부로 수산정책의 일원화, 수산관련 기관간의 상호 유대관계 강화, 수산관련제도 정책의 완화, 연근해 어업의 생산성 복원, 기르는 어업 생산 구조로 변화, 어촌 정주생활권 개발, 수산업 소비구조의 개선, 해외어장의 안정성 확보 등의 정책을 수립하여 미래 수산강국으로써 변모를 가질 수 있는 방안을 제시하였다.

• The Journal of Korean Physical Therapy
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• 제15권3호
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• pp.251-264
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• 2003

Alcohol exposure during development leads to significant long-term neurobehavior dysfunction and central nervous system alteration. The purpose of this study was to determine the effects of enriched enviroment in developmental period through motor behavior test and expression of BDNF. Neonatal rat exposed to alcohol on postnatal days 4 through 10 were studied. Female Sprague-Dawley pups were assigned to two groups. Experimental group(EG) via 4.5 g kg-1day-1 of ethanol was housed in enriched enviornment for 9 weeks. The main result of this study were as follows: 1. There was significant difference in the mean of weight change between control and experimental group. 2. In motor behavior test, there was significant difference in the mean of weight change between control and experimental group. 3. Regarding the immunoreactivity of BDNF were higher appeared experimental group than control group. In conclusion, the present results reveals that enriched enviroment in developmental period is to be extremely useful in neuronal reprganization and motor behavior improvement after alcohol exposure.

• 대한예방한의학회지
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• 제17권2호
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• pp.157-168
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• 2013

The objective of this study was to characterize the adverse effects of Ahnjon-Yichun-Tang during early pregnancy. Following successful mating, female Sprague-Dawley rats were given Ahnjon-Yichun-Tang(AYT) extract by oral administration daily with dose of 150mg (n=10), 300mg(n=10), 450mg(n=10) for 20 days of pregnancy. The rats in Control group(n=10) were orally administrated with Saline. All pregnant rats of Ahnjon-Yichun-Tang-treated and Control groups were sacrificed on day 20 of pregnancy. The pregnancy outcome was determined and the internal and reproductive organs of pregnant rat were observed. The fetuses were examined for the presence of various developmental toxic endpoints and stained with alcian blue and alizarin red S, and observed skeletal malformations. The results obtained in this study represent that there is no significant changes between Control and Ahnjon-Yichun-Tang-treated groups in body weight, organ weight, blood chemistry values, hematological values and pregnancy indexes of pregnant rat. The skeletal malformation of fetus was not observed as well. These results suggest that oral administration of Ahnjon-Yichun-Tang does not produce either maternal or developmental toxicity.

• Toxicological Research
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• 제17권2호
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• pp.97-106
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• 2001

Korean ginseng products have been fumigated with ethylene oxide (EO) for sterilization and prolongation of storage periods. However, there had been controversies indicating that the consumption of food treated with EO might cause harmful effects in human. Since, in Korea the use of EO gas for food treatment was banned in 1991. Since then, irradiation technique has been developed as an alternative. This study was carried out to investigate the effects of irradiated ginseng on fertility, and reproductive and developmental toxicity. Either EO gas fumigated or gamma-irradiated ginseng was administered to male rats by oral gavage for 63 days during the premating period. Female rats were administered from 14 days before mating to day 20 of gestation or to day 21 of lactation. The exposure amount of irradiation used was 5, 10 and 30 kGy, respectively. There were no treatment related changes of darns in clinical signs, and parturition. No treatment related changes in food consumption, body/organ weights, male/female reproductive and fertility performances were observed. F1 fetuses showed no external abnormality. Reflex/sensory junctions, physical/behavioral development, and reproductive performance of F1 rats were not adversary affected. The results of this study show that gamma-irradiated ginseng, up to 30 kGy, has no adverse effects on the fertility, reproduction and development in Wistar rats.

• 한국실내디자인학회논문집
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• 제13호
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• pp.117-123
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• 1997

The purpose of this study was to examine the current interior conditions of kindergarten and to suggest the interior planning guidelines. Data were collected through questionniare survey including observations and interviews. The sample consisted of 262 kindergarten teachers in Pusav. The data were analyzed by using SPSS-PC. The major findings were as follows: (1) Interior finishes should be chosen based on children's behavioral and developmental characteristics. (2) Considering children's safety and teachers' easy access and observation, classrooms and teachers' room should be located at the first floor, and the public spaces such as playroom and assembly room should be located at the top floor because of noise and circulation problem. (3) The size fo the classroom should be increased based on children's age. (4) The teachers were generally satisfied with location, color, and finishes of each room. There was a significant difference between satisfaction of overall characteristics of rooms and theclasses that teachers are in charge of. (5) Children's developmental characteristics can be an important factor when designing a space for childrev. Even though there were some limitations on this study, the findings can be utilized as a basis for generating interior environmental recommendations for planning a better quality of kindergartev.

• 대한상한금궤의학회지
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• 제5권1호
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• pp.61-73
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• 2013

Objective : The purpose of this report is to submit a case study on a patient of developmental disabilities with ADHD by taking "Sihogayonggolmoryeo-tang". Methods : We gave herbal medicine to a patient and observe the progress as the way of Cornners Abbreviated Rating Scale(CARS), ADHD Rating Scale (K-ARS), The ADD-H Comprehensive Teacher's Rating Scale(ACTeRS), Child Attention Problem(CAP), IOWA Conners Rating Scale, Conners Teacher Rating Scale(CTRS), Conners Parents Rating Scale (CPRS), Home Situation Questionnaire(HSQ), School Situation Questionnaire(SSQ). Results : The total score of CARS, ACTeRS and other scales were decreased by taking herbal medicine, and especially hyperactive scores were significantly decreased. Conclusions : Patient's symptom was significantly improved by using "diagnostic system based on Shanghanlun six meridian patterns and provisions" and analyzing patient's psychological and physical conditions.