• Clinical and Experimental Reproductive Medicine
• /
• 제29권1호
• /
• pp.21-28
• /
• 2002

Objective: To evaluate whether co-culture of oocytes on vero cell monolayers from Day 0 (Day 0 group) after egg retrieval results in an increase in developmental capacity such as fertilization rate, embryo quality, blastulation and clinical pregnancy rate compared with co-culture of oocytes from Day 1 (Day 1 group). Methods: Sperms were treated with Hams F-10 supplemented with 10% human follicular fluid (hFF). Vero cells for co-culture were prepared in TCM-199 with 10% FBS. Oocytes were co-cultured from Day 0 and fertilized oocytes were co-cultured from Day 1 on vero cell monolayers in DMEM with 10% and 20% hFF, respectively after egg retrieval. On day 1, 2 and 5, fertilization rate and grade of embryos and blastocysts were evaluated. Results (fertilization rate, cleavage rate, grade of embryos and blastocysts and pregnancy rate) were considered statistically significant when p value was less than 0.05 using t-test and $x^2$. Results: In sibling oocytes of same cycles, no differences were found in fertilization rate (94.6 vs. 91.4%), cleavage rates (94.6 vs. 91.4%), embryo grade (on day 2 and 3) and blastulation (65.6 vs. 57.0%) and their grade. In different oocytes of different cycles (patients), no differences were found in fertilization (79.8 vs. 78.3%), cleavage rates (77.7 vs. 76.4%) and blastulation (56.0 vs. 45.3%), but pregnancy rate was higher in the Day 0 group than in the Day 1 group (60.0 vs. 42.9%). Conclusions: This study revealed that the embryonic development capacities were not affected by the different co-culture time in the sibling oocytes of same cycles. Although no statistical significance, because of small size of study, there was a trend for higher pregnancy rates in Day 0 group compared to Day 1 group in different oocytes of different cycles.

• 한국가축번식학회지
• /
• 제24권4호
• /
• pp.395-406
• /
• 2000

본 연구는 다양한 농도의 FSH 와 LH 에서 배양된 생쥐 preantral follicles 내 난자의 발생능력을 조사하고, 이러한 조건에서 배양된 난자 -난구세포 복합체에서 황체화의 지표인 cytochrome P450 cholesterol side-chain cleavage enzyme (P450scc)와 퇴행화의 지표인 cytochrome P450 17 $\alpha$ -hydroxylase (P450$_{17{\alpha}}$ ) mRNA의 발현정도를 조사하고, 또한 progesterone과 testosterone의 분비농도를 살펴보기 위하여 실시하였다. 체외성장된 난자의 배반포까지의 발달능력은 100 $m\ell$U/$m\ell$ FSH 단독첨가군 (30.2%)과 100 $m\ell$U/$m\ell$ FSH$\pm$l0$m\ell$U/$m\ell$ LH 첨가군 (28.0%)이 100$m\ell$U/$m\ell$ FSH＋100$m\ell$U/$m\ell$ LH 첨가군 (22.0%) 보다 높은 결과를 나타냈다. 그리고 배반포의 평균 세포수에 있어서도 FSH 단독첨가군 (50.9$\pm$26.1)과 100$m\ell$U/$m\ell$ FSH＋10 $m\ell$U/$m\ell$LH 첨가군 (51.0$\pm$26.1)이 100$m\ell$U/$m\ell$ FSH＋100$m\ell$U/$m\ell$ LH 첨가군 (45.2$\pm$15.1) 보다 많은 것으로 조사되었다. 난자 -난구세포 복합체에서 P450scc 와 P450$_{17{\alpha}}$의 발현은 LH의 첨가농도가 증가함에 따라서 증가하였으며, 그리고 progesterone과 testosterone의 분비도 증가를 하였다. 특히, P450scc 와 P450$_{17{\alpha}}$ 의 발현 그리고 progesterone과 testosterone의 분비는 100$m\ell$U/$m\ell$ FSH＋100$m\ell$U/$m\ell$ LH 첨가군에서 다른 첨가군들에 비하여 유의하게 증가하였다. 따라서, 이러한 결과들은 성선자극호르몬이 preantral follicles의 체외배양을 위해서는 필수적이지만, LH 첨가농도의 증가는 난자의 발생능력을 감소시킨다는 것을 보여주었다. 그리고 이러한 결과에 대한 원인의 하나는 황체화의 지표인 P450scc와 퇴행화의 지표인 P450$_{17{\alpha}}$ 발현의 증가에 의한 progesterone과 testosterone의 분비증가에 기인한 것으로 추정된다. 결론적으로, 본 연구는 배양액내에 100$m\ell$U/$m\ell$ FSH 혹은 100$m\ell$U/$m\ell$ FSH+10 $m\ell$U/$m\ell$ LH 의 첨가가 생쥐 preantral follicles의 체외배양을 위한 적정조건임을 제시하고 있다.

• 농촌지도와개발
• /
• 제16권1호
• /
• pp.125-152
• /
• 2009

The objective of this study, which applies the multiple-disciplinary approach to the developmental characteristics of children, is to study and develop a class model that can be applied to actual kindergarten classes in rural area. For this purpose, this study proposes teaching and learning methodologies for children based on English children's tales to help make the English education of children more effective and efficient. Based on the findings, the following suggestions should be considered for improving the English-education class model for kindergartners for rural areas: First, various activities based on the multiple-intelligence approach are important methods of children-oriented education advanced by the Sixth Children's Curriculum, helping children grow their independence and creativity. Second, various activities developed by this study on the basis of the multiple-intelligence approach to promote children's reading, listening, speaking, and writing abilities helped children improve their linguistic capacities, improve creativity, and remain motivated, which was reinforced by the differences found between the test group and the control group.

• 한국가축번식학회지
• /
• 제21권4호
• /
• pp.345-353
• /
• 1997

Antioxidants and antioxidants with somatic cell co-culture, bovine oviduct epithelial cells(BOEC) and buffalo rat liver cells(BRLC), were studied as a mean of increasing the development and intracellular glutathione(GSH) concnetrations of bovine embryos derived from in vitro matured(IVM) and in vitro fertilized(IVF) oocytes. Cell numbers and intracellular GSH concentrations of blastocysts were also counted. The developmental rate beyond morula stages in CRlaa containing taurine(2.5mM), superoxide dismutase(SOD, 600U) and catalase(250U) were 1%, 75.0%, 64.8% and 62.3%, respectively. The developmental rate in antioxidant groups was significantly higher than in control(P<0.05). The intracellular GSH concentrations of blastocysts cultured in 0, 2.5mM taurine, 600U SOD and 250U catalase were 33.8pM, 39.3pM, 42.3pM and 54.8pM, respectively. This result indicated that the developmental rates and intracellular GSH concentrations of catalase group was significantly higher than any other groups(P<0.05). The developmental capacity in CRlaa plus various antioxidants co-cultured with BOEC were 55.3%(control), 74.1%(2.5mM taurine), 66.7%(600U SOD) and 70.7%(250U catalase) and in CRlaa plus various antioxidants co-cultured with BRLC in control, 2.5mM taurine, 600U SOD and 250U catalase were 63.8%, 75.5%, 71.0% and 73.5%, respectveily, the intracellular GSH concentrations of blastocyst embryos co-cultured with BOEC and BRLC in CRlaa with 0.25mM taurine, 600U SOD and 250U catalase were 73.4pM and 64.4pM, 79.9pM and 67.5pM, 82.3pM and 71.7pM, and 83.0pM and 80.0pM, respectively. Cell numbers of blastocysts were not difference in all experimental groups. These studies indicate that andtioxidants and antioxidant with somatic cell co-culture can increase the proportion of embryo that developed into morula and blastocysts, and the intracellular GSH concentrations of blastocyst embryos.

• Asian-Australasian Journal of Animal Sciences
• /
• 제24권2호
• /
• pp.190-197
• /
• 2011

The objective of the present study was to elucidate the fundamental mechanism of bovine oviduct epithelial cell (BOEC) co-culture on developmental capacity of bovine IVM/IVF embryos and to determine whether or not melatonin acts as an antioxidant in BOEC culture and subsequent embryo development. These studies examined the effects of melatonin against NO-induced oxidative stress on cell viability, lipid peroxidation (LPO) and the expression of antioxidant genes (CuZnSOD, MnSOD and Catalase) or apoptosis genes (Bcl-2, Caspase-3 and Bax) during BOECs culture. We also evaluated the developmental rates of bovine IVM/IVF embryos with BOEC co-culture, which were pre-treated with melatonin ($1,000\;{\mu}M$) in the presence or absence of sodium nitroprusside (SNP, $1,000\;{\mu}M$) for 24 h. Cell viability in BOECs treated with SNP (50-$2,000\;{\mu}M$) decreased while melatonin addition (1-$1,000\;{\mu}M$) increased viability in a dose-dependent manner. Cell viability in melatonin plus SNP ($1,000\;{\mu}M$) gradually recovered according to increasing melatonin addition (1-$1,000\;{\mu}M$). The LPO products were measured by thiobarbituric acid (TBA) reaction for malondialdehyde (MDA). Addition of melatonin in BOEC culture indicated a dose-dependent decrease of MDA, and in the SNP group among BOECs treated with SNP or melatonin plus SNP groups MDA was significantly increased compared with SNP plus melatonin groups (p<0.05). In expression of apoptosis or antioxidant genes detected by RT-PCR, Bcl-2 and antioxidant genes were detected in melatonin or melatonin plus SNP groups, while Caspase-3 and Bax genes were only found in the SNP group. When bovine IVM/IVF embryos were cultured for 6-7 days under the BOEC co-culture system pre-treated with melatonin in the presence or absence of SNP, the highest developmental ability to blastocysts was obtained in the $1,000\;{\mu}M$ melatonin group. These results suggest that melatonin has an anti-oxidative effect against NO-induced oxidative stress on cell viability of BOECs and on the developmental competence of bovine IVM/IVF embryo co-culture with BOEC.

• 한국수정란이식학회지
• /
• 제32권3호
• /
• pp.65-71
• /
• 2017

혹서기에 있어 고온 다습한 환경은 동물의 생산성과 생리적 반응에 영향을 주어 HS를 유도하는것으로 알려져 있다. 본 연구에서 HS처리 효과가 도축장 유래 난소에서 채취한 난자의 체외 성숙율, 난할율 및 수정란의 발생능력에 미치는 영향을 비교 검토하였다. 대조군으로서 COCs를 $38.5^{\circ}C$에서 22시간 배양하였으며 실험군은 전배양을 동일하게 21, 18 및 12시간 배양 후 $40.5^{\circ}C$에서 각각 1, 4 및 12시간 동안 후배양하여 HS를 유도하였다. 22 시간 숙성시킨 후, COCs를 체외수정하여 mSOF 배지에서 8 일 동안 배양하였을 때 난자의 성숙율과 수정란의 발생 능력을 조사 하였다. 대조군과 1 및 4 시간동안 HS처리된 난자에서 성숙율과 난할율에는 차이가 없었으나(p > 0.05), 4 시간 HS처리군에서 배반포 형성율이 유의적으로 감소하였다(p < 0.05). 또한, 4시간 이상의 지속적인 HS에 대한 노출은 배반포 형성율과 세포사멸도에 영향을 주는 것으로 관찰되었다(p < 0.05). 이러한 결과는 HS가 난자의 성숙 과정에서 유도되면 수정란의 발생 능력에 부정적인 영향을 줄 수 있음을 시사하며 HS에 의한 소 배반포에서 세포사멸현상이 나타나고 있음을 보여주고 있다.

• 한국수학교육학회지시리즈A:수학교육
• /
• 제56권1호
• /
• pp.119-130
• /
• 2017

The purpose of this study is to explain the long term growth and development of elementary teachers' Professional Learning Communities(PLC) about mathematics implemented on an institutional basis. Especially, it is meaningful to analyze and present the development process and characteristics of PLC, which was started by the basis on the collaboration of a National University of Education and its affiliated elementary school. In this study, PLC activities during three years were analyzed according to the capacities and dimensions of a professional learning community. The developmental capacity of the PLC analyzed in this study can be summarized as follows. In the first year, development of organizational competence in terms of capacity, resources, structure, and system of exchanges was the main factor in personal competence, and the development of individual competence began to share collective learning and practice. In the second year, personal exchanges were active in all the topics of activities, and personal level competence was activated such that more activities of critical knowledge formation were performed on an individual level. On the basis of the development of the individual level formed in the second, individual competence and organizational capacity developed. Factors that have influenced the development of capacities of PLC include: disclosure of activities outside the community, participation in outsiders, provision of procedures to share equal participation and leadership, voluntary and critical participation of teachers, improvement of mathematics teaching methods, sharing themes and visions.

• 보건교육건강증진학회지
• /
• 제16권2호
• /
• pp.187-203
• /
• 1999

This paper will discuss about how we can foster educational support mechanisms to facilitate health promotion programs at the local level. Health promotion in Korea is in the early developmental stage; it has only been since the Health Promotion Act was legislated in 1995, the health promotion programs have been planned and implemented. In the context of the recent decentralization process, local health departments have a major responsibility for developing and implementing health promotion programs at the local level. The short history of health promotion in Korea has meant that local public health departments have limited experience and organizational capacity for health promotion planning ＆ practice. The results of one survey for investigating the progress of health promotion at the local level are instructive. The survey demonstrated that the public health workers recognized that the lack of personnels, insufficient budget, the lack of policy ＆ the organizational support, the lack of skill ＆ knowledge to be effective health promotors, the lack of guidance for health promotion practice were major barriers to implementing health promotion programs at the local level. The object of this paper is to suggest some ways of overcoming barriers to implementing health promotion programs at the local level This paper emphasizes on the importance of educational supports as well as environmental supports - legislative, policy, organizational, economical - in building the organizational capacity and infrastructure of local health department for health promotion. It suggests some ways of providing educational supports to the public health workers at the local level. and supports the positions that educational opportunities for training in health promotion can be better provided to the public health workers at the provincial level rather than at the national level. It argues that the educational ＆ training programs should be developed and based on the educational need assessment; that the application of the main educational principles ＆ theoretical models for health promotion be used to develop educational programs for the public health workers; and that professional health organizations should make plans to provide more educational programs at their annual conferences or at other convenient times. These kinds of educational supports facilitate the ability of public health workers to improve their capacity for health promotion practice at the local level and help to alleviate some of the pressure on state resources.

• 한국보건교육건강증진학회:학술대회논문집
• /
• 한국보건교육건강증진학회 1999년도 3rd International Symposium
• /
• pp.165-183
• /
• 1999

This paper will discuss about how we can foster educational support mechanisms to facilitate health promotion programs at the local level. Health promotion in Korea is in the early developmental stage; it has only been since the Health Promotion Act was legislated in 1995, the health promotion programs have been planned and implemented. In the context of the recent decentralization process, local health departments have a major responsibility for developing and implementing health promotion programs at the local level. The short history of health promotion in Korea has meant that local public health departments have limited experience and organizational capacity for health promotion planning ＆ practice. The results of one survey for investigating the progress of health promotion at the local level are instructive. The survey demonstrated that the public health workers recognized that the lack of personnels, insufficient budget, the lack of policy ＆ the organizational support, the lack of skill ＆ knowledge to be effective health promotors, the lack of guidance for health promotion practice were major barriers to implementing health promotion programs at the local level. The object of this paper is to suggest some ways of overcoming barriers to implementing health promotion programs at the local level This paper emphasizes on the importance of educational supports as well as environmental supports - legislative, policy, organizational, economical - in building the organizational capacity and infrastructure of local health department for health promotion. It suggests some ways of providing educational supports to the public health workers at the local level. and supports the positions that educational opportunities for training in health promotion can be better provided to the public health workers at the provincial level rather than at the national level. It argues that the educational ＆ training programs should be developed and based on the educational need assessment; that the application of the main educational principles ＆ theoretical models for health promotion be used to develop educational programs for the public health workers; and that professional health organizations should make plans to provide more educational programs at their annual conferences or at other convenient times. These kinds of educational supports facilitate the ability of public health workers to improve their capacity for health promotion practice at the local level and help to alleviate some of the pressure on state resources.

• 수산해양교육연구
• /
• 제29권3호
• /
• pp.899-908
• /
• 2017

The purpose of this study is to identify challenges related to social, economic and environmental change for fishing villages and suggest the developmental direction of fishing village to actively respond to new changes. This study mainly focuses on fishing village fraternities. Its current problems are summarized as follows: shortage of labor, population aging, entry barrier against newcomers, insufficient guidance and supervision functions, lack of funds of new projects and inadequate statistics and data. The fishing village community in the modern meaning of the term can be understood as an economic and flexible social-cultural community based on openness, cooperation instead of exclusiveness and closeness, which aligns fisheries with local industries. Thus, in order for the fishing village community to improve, the followings areas required. First, strengthening capacity of the fishing village community. Second, establishing the database system and the third, reorganizing the fishing village community into a sea village community.