• 한국축산식품학회지
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• 제36권4호
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• pp.458-462
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• 2016

The effect of ultra-sonication on quality characteristics and flavor of baked eggs was studied. One hundred and twenty eggs were cooked and assigned to six treatments groups (n=20 each) that were then soaked in saline solution at various concentrations (5, 10 and 18%) with/or without further ultra-sonication treatment at 35 kHz for 1 h. The pH values were lower in the ultra-sonicated samples in comparison with the non-ultra-sonicated samples (p<0.05). The values for texture traits were higher in the samples soaked in 10% saline solution with ultra-sonication in comparison with other remaining treatments or control (p<0.05). The sodium content in samples soaked in 10% saline solution with ultra-sonication was similar to that of the ones soaked in 18% saline solution without ultra-sonication. The higher flavor scores were also given for the ultra-sonicated samples in comparison with the control or non-ultra-sonicated ones. These results suggest that the application of ultra-sonication may produce a faster sodium penetration into baked eggs, simultaneously improves some textural traits (e.g., hardness) as well as flavor of the products.

• 한국식품위생안전성학회지
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• 제4권1호
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• pp.29-36
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• 1989

In order to investigate the Storage time of chicken eggs, several physico-chemical tests from chicken eggs store at 5, 13 and 27$^{\circ}C$ were examined. Egg samples were collected from six poultry farms. Egg stored at 5$^{\circ}C$ based on the depth of air cell and specific gravity, were all acceptable until 17 days ; on the egg yolk coefficient and pH of the egg white and egg yolk until about 10 days. Egg stored at 13$^{\circ}C$, based on the depth of air cell. were acceptable by about 10 days of storage, but on the other physico-chemical tests by about 7 days. Egg samples stored at room temperature(about 27$^{\circ}C$) base on the depth of air cell, were acceptable by about 5 days of storage ; on the specific gravity by 4 days ; and on the egg yolk coefficient and pH of the egg yolk and egg white by 3 days. The results of this study showed that egg stored at 5$^{\circ}C$ were considered acceptable by 10 days of storage ; at 13$^{\circ}C$ by 7 days ; at room temperature (27$^{\circ}C$) by 3 days.

• 한국수산과학회지
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• 제47권5호
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• pp.654-658
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• 2014

We report the first identification of a spawning ground of the blackfin flounder Glyptocephlaus stelleri near the Wangdol-cho sea mountains, located in the southern East Sea. Eggs and larval fish of G. stelleri were collected during April and June, 2014, when an abundance of eggs was found in the southern area of Wangdol-cho. Our findings suggest that G. stelleri prefers to spawn in the vicinity of the off-shore sea mountains, where the temperature is between 10 and $12^{\circ}C$ and the water depth is 100 m, rather than inshore.

• International Journal of Industrial Entomology and Biomaterials
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• 제15권2호
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• pp.165-169
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• 2007

A differential specific pattern of variation in the metabolism of protein and cholesterol was noticed in non -diapause and diapause eggs due to the significant differences in embryonic development. The rate of metabolism was different due to specific demands of such metabolites during active embryogenesis and maintenance of diapause respectively. In general, the metabolic rate was found to be accelerated in non- diapause eggs just after egg deposition, while it was very slow in diapause eggs. When the diapause eggs were treated with hydrochloric acid within 16-20 hrs, the rate of turnover was found to very similar to non- diapause eggs, though the base level of protein and cholesterol was recorded to be different.

• 대한수의학회지
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• 제32권4호
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• pp.663-669
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• 1992

This experiment was carried out to find out the best condition for the parthenogenetic activation of mouse eggs by treating ethanol and hyaluronidase. For the parthenogenetic activation of eggs with ethanol, cumulus cell enclosed or denuded eggs were treated with 7% ethanol in D-PBS for 5, 7 or 9 minutes. For the activation of eggs with hyaluronidase, the eggs with cumulus masses were released into D-PBS with 100 unit hyaluronidase and treated for 10, 12 or 13 minutes. All of the treated eggs were incubated in BMOC-3 solution for 5 hours at $37^{\circ}C$ at an atmosphere of 5% $CO_2$ in air. The types of parthenogenetic eggs were morphologically classified into haploid, diploid, immediate cleavage eggs under an inverted microscope. The results obtained in this experiment were summarized as follows ; 1. High activation rate(99%) had been achieved by treating the eggs with 7% ethanol for 7 minutes. 2. With 100 IU hyaluronidase, high activation rate (94%) had been achieved by treating for 12 minutes. 3. The most frequent type of parthenogenetic eggs activated with ethanol or hyaluronidase was haploid (p<0.05). 4. The eggs collected from 18 to 22 hours post HCG injection showed higher activation rate than the eggs collected at 16 hours post HCG injection. 5. No significant difference (p>0.05) in activation rate was shown in strain of mouse and in presence of cumulus cells.

• Parasites, Hosts and Diseases
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• 제50권3호
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• pp.239-242
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• 2012

The influence of temperature on the development and embryonation of Ascaris suum eggs was studied using coarse sand medium in an environmental chamber with 50% humidity. The time required for development and embryonation of eggs was examined under 3 different temperature conditions, $5^{\circ}C$, $25^{\circ}C$, and $35^{\circ}C$. A. suum eggs did not develop over 1 month at the temperature of $5^{\circ}C$. However, other temperature conditions, $25^{\circ}C$ and $35^{\circ}C$, induced egg development to the 8-cell-stage at days 5-6 after incubation. All eggs examined developed to the 8-cell stage at day 6 after incubation in the sand medium at $25^{\circ}C$. The higher temperature, $35^{\circ}C$, slightly accelerated the A. suum egg development compared to $25^{\circ}C$, and the development to the 8-cell stage occurred within day 5 after incubation. The formation of larvae in A. suum eggs at temperatures of $35^{\circ}C$ and $25^{\circ}C$ appeared at days 17 and 19 after incubation, respectively. These findings show that $35^{\circ}C$ condition shortens the time for the development of A. suum eggs to the 8-cell-stage in comparison to $25^{\circ}C$, and suggest the possibility of accelerated transmission of this parasite, resulting from global warming and ecosystem changes.

• 한국가축번식학회지
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• 제17권4호
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• pp.365-373
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• 1994

To investigate the effect of EDTA on the in vitro development of parthenogenetic eggs of ICR strain mice, those were cultured in 35mm culture dishes containing NaHCO3-BMOC-3 medium supplemented with 10, 50, 100, or 500$\mu$M of EDTA at 37$^{\circ}C$ for 96hrs. under the atmosphere of 5% CO2 and 95% air. EDTA supplementation of 10, 50, or 100$\mu$M to medium significantly(P<0.01) increase morula and blastocyst formation rate compared with controls in haploid(19.8, 25.9, 39.0% vs. 0.0%). And compared with 10, or 50$\mu$M of EDTA supplementation, significantly(P<0.01) higher morula and blastocyst formation rate resulted from EDTA supplementatin of 100$\mu$M. Both the nuclear number and diameter of blastocysts developed from parthenogenetic eggs were not affected by the morphological types when they were cultured, or the supplementary concentrations of EDTA. The nuclear number of blastocysts developed from haploid, diploid, and immediately cleavaged eggs was 44.8$\pm$1.2, 45.2$\pm$1.5, and 45.4$\pm$1.8, respectively. And the diameter of those eggs ranged 104.4$\pm$1.8, 104.3$\pm$1.2, and 103.8 1.3$\mu$m, respectively.

• 한국응용곤충학회지
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• 제30권1호
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• pp.37-41
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• 1991

토양수분함량과 큰검정풍뎅이의 밀도 변화와의 관계를 파악하는데 필요한 기초 자료를 얻기 위해 토양수분함량이 큰 검정풍뎅이의 난과 유충의 생존에 미치는 영향을 조사하였다. 사양토와 식양토의 수분함량 15%와 25%에서는 난과 1, 2, 3령 유충 모두 79% 이상의 생존율을 보였으나 5%와 35%에서는 현저히 떨어졌다. 이 극한 수분함량에서는 토성에 따라 난과 유충의 생존율에 차이가 있는 것으로 보였다. 큰검정풍뎅이의 난은 생존할 수 있는 낮은 수분함량의 한계에 가까워질수록 발육기간이 길어졌다. 식양토에서 33-36%의 높은 수분함량에 난을 일정 기간 처리했을 때 배자 발육을 진행된 난일수록 잘 견뎠고 처리기간이 길어짐에 따라서 전란기간이 길어졌다. 또한 높은 토양수분함량에서는 3령 유충의 섭식 활동이 뚜렷이 억제되었다.

• 한국축산식품학회지
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• 제31권4호
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• pp.557-562
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• 2011

본 연구는 국내에서 유통되고 있는 액란의 냉장저장 중 미생물 및 이화학적 성상을 구명하기 위해 수행하였다. 본 실험에 사용된 액란은 살균 및 비살균 처리된 전란액, 난황액 및 난백액으로 여름철과 겨울철 생산 제품에 대해 각각 조사하였다. 냉장저장 중 세균수의 경우 여름철에 생산된 비살균 액란(전란액, 난황액 및 난백액)은 1,270-83,300 CFU/g 수준으로 겨울철에 생산된 비살균 액란의 0-4,330 CFU/g 에 비해 증가하는 것으로 나타났다. 살균 전란액과 살균 난황액에서는 저장기간 동안 액란생산 계절과 관계 없이 대장균군이 검출되지 않는 것으로 나타났다. 반면, 비살균 액란은 냉장저장 중 겨울철에 생산된 난백액에서만 대장균군이 검출되지 않는 것으로 나타났다. Salmonella는 살균 및 비살균 액란 모두에서 냉장저장 중 액란 생산 계절과 관계없이 검출되지 않았다. 그러나, 여름철에 생산된 비살균 전란액으로부터 Salmonella 의심균을 동정한 결과, Pseudomonas sp, Pseudomonas geezenne, Pseudomonas otitidis, Pseudomonas aeruginosa인 것으로 판명되었다. 액란의 냉장저장 중 pH 및 점도변화는 살균과 비살균 처리구간 뚜렷한 차이가 없는 것으로 나타났다. 이상의 결과를 종합해 볼 때 여름철에 비살균 액란 생산시 교차오염에 각별한 주의가 필요할 것으로 사료된다.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2001년도 춘계학술발표대회
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• pp.6-6
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• 2001

Oocyte freezing has become a prevalent source for related reproductive technologies. This study was carried out to evaluate viability of post-thawed bovine oocyte injected DTT-treated sperm following by two different activation stimuli (Group 1, 5 M ionomycin, 5 min ＋ CR1aa, 3 h . 1.9 mM dimetylaminopurine (DMP), 3 h; group 2, ionomycin ＋ 10 $\mu\textrm{g}$/$m\ell$ cycloheximide(CHX), 5h). The techniques of ultra-rapid freezing used in this study were essentially similar to those of described by Vajta et al (Theriogenology 1999; 52:939-948), Denuded oocytes at 22 h of culture were exposed to cryoprotectant (3.2 M Ethylene glycol, 2.36 M DMSO, 0.6 M sucrose), and followed by freezing in electron microscopic grid. After thawing the oocytes were transferred back into the drop of maturation medium and cultured for additional 2 h before being subjected to ICSI. All eggs were then cultured in CRlaa medium, and transferred into M199+10% FCS on day 4. The culture was maintained until day 9. In Experiment 1, frozen-ICSI eggs were compared on development into blastocyst to those of unfrozen and IVF control. Those eggs were activated with the method of group 2. A higher proportion of unfrozen-ICSI and IVF eggs developed into cleavage and blastocysts than of frozen-ICSI eggs (65% and 13%; 71% and 23% vs. 39% and 8%; P<0.05). In Experiment 2, development and ploidy of embryos made from group 1 were compared to those from group 2. Between groups there did not differ on the rates of development, however, chromosomal abnormality in group 1 was significantly higher than in group 2 (49% vs. 30%; P<0.05). The present result suggests that frozen bovine oocytes can be used for ICSI.