• Biomolecules & Therapeutics
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• v.20 no.1
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• pp.27-35
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• 2012

Oroxylin A is a flavone isolated from a medicinal herb reported to be effective in reducing the inflammatory and oxidative stresses. It also modulates the production of brain derived neurotrophic factor (BDNF) in cortical neurons by the transactivation of cAMP response element-binding protein (CREB). As a neurotrophin, BDNF plays roles in neuronal development, differentiation, synaptogenesis, and neural protection from the harmful stimuli. Adenosine $A2_A$ receptor colocalized with BDNF in brain and the functional interaction between $A2_A$ receptor stimulation and BDNF action has been suggested. In this study, we investigated the possibility that oroxylin A modulates BDNF production in cortical neuron through the regulation of $A2_A$ receptor system. As expected, CGS21680 ($A2_A$ receptor agonist) induced BDNF expression and release, however, an antagonist, ZM241385, prevented oroxylin A-induced increase in BDNF production. Oroxylin A activated the PI3K-Akt-GSK-$3{\beta}$ signaling pathway, which is inhibited by ZM241385 and the blockade of the signaling pathway abolished the increase in BDNF production. The physiological roles of oroxylin A-induced BDNF production were demonstrated by the increased neurite extension as well as synapse formation from neurons. Overall, oroxylin A might regulate BDNF production in cortical neuron through $A2_A$ receptor stimulation, which promotes cellular survival, synapse formation and neurite extension.

• Journal of Plant Biotechnology
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• v.38 no.2
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• pp.162-168
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• 2011

High salinity is a common stress condition that adversely affects plant growth and crop production. In response to various environmental stresses, plants activate a number of defense genes that function to increase the tolerance. To isolate Arabidopsis genes that are involved in abiotic stress responses, we carried out genetic screening using various mutant lines. Among them, the blh8 ($\b{B}$EL1-$\b{L}$ike $\b{H}$omeodomain $\underline{8}$) mutant specifically shows chlorotic phenotypes to ionic (specifically, $Na^+$ and $K^+$) stresses, but no differences in root growth. In addition, BLH8 is related to plant development and abiotic stress as predicted by a Graphical Gaussian Model (GGM) network program. It implies that BLH8 functions as a putative transcription factor related to abiotic stress responses. Collectively, our results show that gene network analysis is a useful tool for isolating genes involved in stress adaptation in plants.

• Korean Journal of Veterinary Research
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• v.52 no.3
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• pp.183-191
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• 2012

The maintenance of peripheral immune tolerance and prevention of chronic inflammation and autoimmune disease require $CD4^{+}CD25^{+}$ T cells (regulatory T cells). The transcription factor Foxp3 is essential for the development of functional, regulatory T cells, which plays a prominent role in self-tolerance. Retroviral vectors can confer high level of gene transfer and transgene expression in a variety of cell types. Here we observed that following retroviral vector-mediated gene transfer of Foxp3, transductional Foxp3 expression was increased in the liver, lung, brain, heart, muscle, spinal cord, kidney and spleen. One day after vector administration, high levels of transgene and gene expression were observed in liver and lung. At 2 days after injection, transductional Foxp3 expression level was increased in brain, heart, muscle and spinal cord, but kidney and spleen exhibited a consistent low level. This finding was inconsistent with the increase in both $CD4^{+}CD25^{+}$ T cell and $CD4^{+}Foxp3^{+}$ T cell frequencies observed in peripheral immune cells by fluorescence-activated cell-sorting (FACS) analysis. Retroviral vector-mediated gene transfer of Foxp3 did not lead to increased numbers of $CD4^{+}CD25^{+}$ T cell and $CD4^{+}Foxp3^{+}$ T cell. These results demonstrate the level and duration of transductional Foxp3 gene expression in various tissues. A better understanding of Foxp3 regulation can be useful in dissecting the cause of regulatory T cells dysfunction in several autoimmune diseases and raise the possibility of enhancing suppressive functions of regulatory T cells for therapeutic purposes.

• Proceedings of the Korea Water Resources Association Conference
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• 2007.05a
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• pp.1076-1080
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• 2007

최근 국내에서는 하천변 생태계를 대상으로 생태구조 및 서식처 평가 등 많은 연구가 진행되었음에도 불구하고 생태적 변화를 예측하고 예측된 결과에 따라 생태복원 대책을 수립할 수 있는 연구는 현재까지 미흡하다고 볼 수 있으며, 이는 생태복원 연구에 있어 주요과제라고 할 수 있다. 이에 반해 유럽과 미국에서는 수문학적 서식처 조건에 따른 식생 반응 예측 등 수문생태 모형에 대한 연구가 꾸준히 이루어져 왔으며, 최근에는 습지, 홍수터 등을 대상으로 생태보전 복원분야에 널리 응용되고 있다. 본 연구에서는 국외의 수문생태모형 적용사례를 분석하여 국내 적용가능성 및 문제점에 대한 타당성을 제시하고자 하였다. 그 대상으로는 유역 내에 홍수량 분담을 위한 천변저류지를 적용하였으며, 의미상으로 홍수터(범람원), 습지 역시 해당 범위에 포함할 수 있다. 천변저류지는 홍수기와 비홍수기에 따라 활용 측면을 구분할 수 있으며, 이런 맥락에서 천변저류지의 수위변화는 식생 변화의 중요한 요소(factor)가 될 수 있다. 그 외 범람시기, 범람일수, 건조기간 등을 포함한 수문요소 역시 수문생태 모델의 예측 변수인 식생 성장에 영향을 미치게 된다. 이러한 수문/생태시스템의 상호관계를 활용하고 모형의 형태를 국내 식생에 대한 생리학적 특성에 맞게 변화시킬 수 있다면 국내 천변저류지에도 충분히 적용 가능할 것으로 판단된다. 또한 잠재적으로 식생 성장에 영향을 줄 수 있는 다양한 환경조건 예를 들면 영양물질, 토양구조, 토사 퇴적 등을 적용대상지 환경에 맞게 포함하거나 고려할 수 있다면 모형의 재현성을 더욱 높일 수 있을 것이다.의 기대효과를 가져올 수 있으리라 생각되며 분석된 인자들은 수달 서식지를 위한 하천정비의 기초자료에 유용할 것으로 보인다.따른 유량측정망을 구축하는 것이다.의 의사결정 지원 도구가 될 것이다. 따라서, 본 연구에서는 도시유역의 물순환 해석을 위한 일련의 과정, 즉 자료의 조사 및 취득에서부터 물순환 해석 모형을 이용한 정량적 현황파악, 물순환 개선 기법 및 평가를 수행함에 있어 주요 착안점 및 실무에서의 기술적 가이드를 제공하고자 하였으며, 보다 세밀한 도시유역의 물순환 해석을 위하여 우리나라와 일본에서 적용이 활발한 물리적 기반의 분포형 모형(WEP, SHER, SWMM)의 적용사례를 통하여 국내 도시하천의 물순환 해석에 활용함에 있어서의 실질적인 적용절차 등을 제시하고자 하였다. 한다.호강유역의 급격한 수질개선을 알 수 있다.世宗實錄) $\ulcorner$지리지$\lrcorner$(地理志)와 동년대에 동일한 목적으로 찬술되었음을 알 수 있다. $\ulcorner$경상도실록지리지$\lrcorner$(慶尙道實錄地理志)에는 $\ulcorner$세종실록$\lrcorner$(世宗實錄) $\ulcorner$지리지$\lrcorner$(地理志)와의 비교를 해보면 상 중 하품의 통합 9개소가 삭제되어 있고, $\ulcorner$동국여지승람$\lrcorner$(東國與地勝覽) 에서는 자기소와 도기소의 위치가 완전히 삭제되어 있다.

• Korean Journal of Medicinal Crop Science
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• v.11 no.2
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• pp.161-166
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• 2003

Korean ginseng(Panax gmseng C.A. Meyer) is very difficult to obtain stable production of qualified ginseng roots because of variable stresses in soil environments. In environment stresses, soil condition is the most important factor, among which nutrients, especially inorganic materials such as N, P, K, Ca, Mg, Fe, etc., influence greatly on the ginseng growth. However, present ginseng field soils in Korea contain so much amount of such inorganic materials that a variety of remarkable disorders were noted in many ginseng plantations, resulting in decrease of qualitative ginseng production. Therefore, it is required to search for genetic resources and genes tolerant to salt stress for the development of ginseng cultivars. Selection of stress-tolerant ginseng lines in fields is very difficult because it is almost impossible to control properly the environmental conditions of soil. On the contrary, it can be studied with ease to search for stress-tolerant ginseng lines through in vitro culture because of easy manipulation of stress conditions. Murashige & Skoog(MS) media with 2.5 folds of $KNO_3,\;NH_4NO_3,\;MgSO_4\;7H_2O,\;KH_2PO_4,\;and\;CaCl_2\;2H_2O$ was established for the selection of ginseng lines tolerant to salt stress under the embryo culture.

• Development and Reproduction
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• v.19 no.4
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• pp.243-252
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• 2015

The pregnancy and abortion process involves a complex mechanism with various immune cells present in the implantation sites and several hormones associated with pregnancy, such as leptin, ghrelin and nesfatin-1. However, the mechanism underlying spontaneous abortion by maternal T helper 17 (Th17) present in the implantation sites and nesfatin-1, which is of anorexigenic hormones, is not fully understood so far. Therefore, the purpose of this study was to examine the possible roles of Th17 cells present in the implantation sites and nesfatin-1 expressed in the uterus on spontaneous abortion using the $CBA/j{\times}DBA/2$ mouse model. Th17 transcription factor, ROR-${\gamma}t$ mRNA expression was significantly increased in the abortion sites compared with the implantation sites of abortion model mice on day 14.5 and 19.5 of pregnancy. In addition, the expression levels of IL-17A mRNA were significantly higher in abortion sites than in implantation sites on day 14.5 and 19.5. Moreover, the nesfatin-1/NUCB2 protein and mRNA levels were increased in abortion sites compared with levels in implantation sites of both normal pregnant and abortion model mice on day 14.5 of pregnancy. Interestingly, nesfatin-1/NUCB2 serum levels were not changed throughout the whole pregnancy in abortion model mice, but its serum level was dramatically increased on day 14.5, and then rapidly decreased on day 19.5 in normal pregnant mice. In this study, we showed for the first time the expression of nesfatin-1/NUCB2 mRNA and protein in implantation sites during pregnancy. The present results suggest that Th17 cells in the uterus may play an important role in the period of implantation and for maintenance of pregnancy. Furthermore, the present results suggest that Th17 cells in implantation sites may be a key regulator for maintenance of pregnancy and provides evidence that activation of these cells may be regulated by nesfatin-1/NUCB2. Further study is needed to elucidate the role of nesfatin-1 expressed in the uterus during pregnancy.

• Journal of Engineering Education Research
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• v.4 no.1
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• pp.57-78
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• 2001

Due to developments in information technology and ensuing changes in the concept of time and space, the significance of national boundaries has been weakened, bringing to attention the heightened competition among nations. In order to respond to these transformations, the Korean government is increasing its efforts to raise the authenticity of the National Technology Qualification System(NTQS) by closely linking NTQS testing method and procedure to practical knowledge and skills in the industry field. This initiative is based on the recognition that the human resources development is a crucial factor in determining a nation's competitiveness. This study aimed, first of all, to diagnose the problems directly and indirectly affecting the testing method in the current NTQS. And then, differentiated solutions according to grade and improvements in the testing infrastructure were suggested. The ultimate purpose was to provide a blueprint for enhancing national competitiveness through increased authenticity and efficiency of the NTQS. Research methods applied during this study include: the collection and review of qualification-related literature; a series of conferences with leading experts; interviews with vocational educators and researchers from qualification-related institutes; a survey of national qualification holders from a variety of professions and industries; and participatory observation at qualification exam centers. This research suggested policy recommendations as follows; First, measures to improve for testing method of professional engineer Second, measures to improve for testing method of Master craftsman Third, measures to improve for testing method of Engineer, Industrial Engineer, Craftsman Fourth, plans for the establishment of operation system for a computerized testing Fifth, institutional perspective on establishing qualification infrastructure Sixth, operational perspective on establishing qualification infrastructure

• Science of Emotion and Sensibility
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• v.9 no.2
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• pp.133-140
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• 2006

In order to investigate the sensation of the fabric sound simulating the real wear-condition, the fabric sound simulator using reciprocating friction was developed. Fabric sounds from 5 specimen were generated by the fabric sound simulator and recorded using high performance microphone. Physical sound parameters of fabrics including level pressure of total sound (LPT), level range (${\Delta}L$), and frequency differences (${\Delta}f$) were calculated. For psychological evaluation, seven adjectives for sound (softness, loudness, sharpness, clearness, roughness, highness, and pleasantness) were used as the semantic differential scale. Fabric sounds by reciprocating friction of nylon taffeta and polyester leno had the highest value of LPT and evaluated as loud, sharp, rough, and unpleasant while polyester ultra suede and silk crepe de chine haying the lower LPT and ${\Delta}f$ were perceived as soft and quite. Comparing with fabric sound by one-way friction, fabric sound by reciprocation friction was perceived as more sharp, loud, and rough. LPT was also the most important factor affecting the sensation of the fabric sound by reciprocating friction.

• Journal of Internet Computing and Services
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• v.14 no.5
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• pp.27-38
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• 2013

Currently a number of managers exist to manage heterogeneous networks, in this situation, the NETCONF protocol for efficient network management has been proposed as a new protocol. However, the standard NETCONF protocol stack continuous improvement since the establishment but in four layers still have some problems. Especially in situations where there are multiple administrators, problems are more highlighted in operation layer. In this paper, we focus on these issues and the Operation layer has improved the efficiency and flexibility of operations among NETCONF four layers. Additionally, for the inefficiency of updates improved the device settings based on improved operation techniques. In addition, standard protocol NETCONF did not proposed content layer data structure and we propose standard technique of content layer that can generate configuration structure of devices. Improved the three techniques are applied appropriately to the NETCONF, the proposed method and the existing NETCONF was performed experiment to compare with experimental four factors. Compare key factor are four kind as maintaining the probability of network function, the reaction performance about command, the number of control packets, performance of data creation in content layer. Such factors after performing the experiment, the proposed method in this paper is superior to the existing NETCONF and there was confirmed by analysis Experimental results.

• Korean Journal of Ecology and Environment
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• v.50 no.3
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• pp.286-294
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• 2017

Development of transportation is rapidly reducing the barriers between countries, but this is causing the easier migration of species than the past. Typically, exotic species are imported for the purpose of food, leather, and pets. However, it has been introduced into the wild through artificially or naturally paths, and recently they are become a main cause of ecosystem disturbance. In this study, we investigated exotic turtle species introduced into the wild and analyzed their distribution characteristics. As a result of filed surveys, totally 4 genus 8 species 62 individuals of exotic turtles were found from 126 reservoirs in Gangwon-do and Gyeongsangnam-do, South Korea. In particular, Trachemys scripta elegans showed relatively high frequency than other turtle species and the natural reproduction of T. s. elegans was confirmed in some areas. On the other hand, the frequency of discovery of exotic turtles except T. s. elegans was relatively low, and the range of the areas was limited. Especially, exotic turtles except T. s. elegans were mainly found in public places such as reservoirs in the park. As a result of analyzing the distribution characteristics of exotic turtles using geographic information system, the turtles' distribution showed a high correlation with the artificial factor such as "urban". In this study, we identified the distribution characteristics of exotic turtles in the Korean wild, and these results will be important data for understanding status the and establishing effective management methods for exotic species.