• Journal of Life Science
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• v.25 no.9
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• pp.1007-1013
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• 2015

A COG (Cluster of Orthologous Groups of proteins) algorithm was applied to detect conserved genes in 711 prokaryotes. Only COG0080 (ribosomal protein L11) was common among all the 711 prokaryotes analyzed and 58 COGs were common in more than 700 prokaryotes. Nine COGs among 58, including COG0197 (endonuclease III) and COG0088 (ribosomal protein L4), were conserved in a form of one gene per one organism. COG0008 represented 1356 genes in 709 of the prokaryotes and this was the highest number of genes among 58 COGs. Twenty-two COGs were conserved in more than 708 prokaryotes. Of these, two were transcription related, four were tRNA synthetases, eight were large ribosomal subunits, seven were small ribosomal subunits, and one was translation elongation factor. Among 58 conserved COGs in more than 700 prokaryotes, 50 (86.2%) were translation related, and four (6.9%) were transcription related, pointing to the importance of protein-synthesis in prokaryotes. Among these 58 COGs, the most conserved COG was COG0060 (isoleucyl tRNA synthetase), and the least conserved was COG0143 (methionyl tRNA synthetase). Archaea and eubacteria were discriminated in the genomic analysis by the average distance and variation in distance of common COGs. The identification of these conserved genes could be useful in basic and applied research, such as antibiotic development and cancer therapeutics.

• Journal of Life Science
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• v.28 no.6
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• pp.733-737
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• 2018

Cucurbitacin-I, a natural triterpenoid derived from Cucurbitaceae family plants, exhibits a number of potentially useful pharmacological and biological activities. Indeed, the previous study demonstrated that cucurbitacin-I reduced the proliferation of colon cancer cells by enhancing apoptosis and causing cell cycle arrest at the G2/M phase. CD44, a type I transmembrane protein with the function of adhering to cells, mediates between the extracellular matrix and other cells through hyaluronic acid. Recent studies have demonstrated that an overexpression of the CD44 membrane receptor results in tumor initiation and growth, specific behaviors of cancer stem cells, the development of drug resistance, and metastasis. The aim was to examine the effect of cucurbitacin-I on CD44 expression human ovarian cancer cells because the effect of cucurbitacin-I on CD44 expression has not been reported. The expressions of CD44 mRNA and protein were detected using a quantitative real-time reverse-transcription polymerase chain reaction and a Western blot analysis, respectively. Treatment with cucurbitacin-I inhibited the expression of CD44 mRNA and protein. A subsequent analysis revealed that cucurbitacin-I blocked the phosphorylation of activator protein-1 (AP-1) and nuclear factor kappa-B ($NF-{\kappa}B$), which are key regulators of CD44 expression. Taken together, the data demonstrate that cucurbitacin-I regulates the AP-1 and $NF-{\kappa}B$ signaling pathways, leading to decreased CD44 expression.

• Asia pacific journal of information systems
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• v.20 no.2
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• pp.157-180
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• 2010

Rapid development of information technology has generated a new industry and market. In particular, network technology such as the Internet and other computer networks made transaction activities switched from traditional offline commerce to e-commerce. Among them, digital content is bit-based object which is created and distributed through electronic environment. In particular, many entertainment contents such as the music, the movies, and the computer game softwares are main products. Although digital content commerce has high potential demand, it lacks the consideration about the factor related to maintaining existing customer such as customer loyalty and switching costs. There has been a number of research on customer loyalty and other factors affecting it in the traditional electronic commerce environment, but there is a lack of research which examines the characteristics of digital content. The study about the effect of switching costs on customer loyalty in digital content commerce is necessary because the customers of digital content commerce market are from those of other e-commerce market or traditional offline commerce market. In addition, customer loyalty and switching costs are important factors because they may build up greater customer retention. For that reason, this study focused on examining the relationships among switching costs, antecedents for switching costs, and customer loyalty in online digital music service industry. The study has three major purposes: (1) to find antecedents of switching costs on digital content commerce and examine effect of antecedents for switching costs; (2) to identify effect of switching costs on customer loyalty in digital content commerce and examine moderating effects of alternative attractiveness; (3) to identify the differences of antecedents for switching costs by contents transmission type(streaming service and downloading service). And, the online digital music service industry is selected in this study since there are many users and transactions incurring. To accomplish these purposes, a survey questionnaire was developed and distributed to 256 informants. Survey instrument was developed based on previous research and pre-established survey items. Total of 206 surveys are collected and used in the data analysis. Among the respondents, 56.8% is male and 43.2% is female. Also, 86 responses were streaming service user group and 120 responses were download service user group. These data was analyzed using regression analysis. Major findings of empirical analysis can be summarized as follows. First, switching costs have positive effect on customer loyalty in digital content commerce environment. Second, the influence of switching costs on customer loyalty increases under conditions of high alternative attractiveness. Third, DRM convenience and breadth of use have positive effect on switching costs. The findings imply that the digital content provider should pay more attention to switching costs in addition to customer satisfaction in order to attract customers. Also, increasing the convenience of DRM use by securing the convenience of user interface and expanding the support device and increasing the service use scope by providing diverse value-added service helps to create a switching barrier. The result of the study can become a practical use in marketing strategy for maintaining existing customer. In particular, switching barrier is very important under conditions of high competition in the online music service market. This study can be used as a basis for further studies about customer retention in digital content commerce.

• Analytical Science and Technology
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• v.24 no.6
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• pp.503-509
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• 2011

Specific surface area is becoming a very important factor when newly developed advanced nano-materials are evaluated. But there have been many differences in results when measuring specific surface areas, depending on the measuring equipments and analysis method. To verify the reliability of the specific surface area measurement device supplied within the country, Round Robin Test (RRT) has been done at 21 affiliated research institutes. As a result, it was found that several institute had problems in measuring of gas adsorption amount in measuring equipment, and this proved the need for certified reference material (CRM). Furthermore, it was also found that the results from BET analysis is easily swayed by the analyst's subjectivism, and the calculated results may differ up to 16% in case of CRM I depending on the selection range of BET analysis. So this showed that a standard guideline for BET constant C value and fitting correlation coefficient R is needed, to properly select range in BET analysis. The experience in RRT, distribution of CRM, and standardized procedure would result in improved reliability in industrial processes, and thus, would contribute to the quality management, the productivity improvement, the safety evaluation, and the new material development.

• Journal of Embryo Transfer
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• v.33 no.3
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• pp.129-137
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• 2018

Acute vascular rejection has been known as a main barrier occurring in a xenograted tissue of alpha 1,3-galactosyltransferase knock-out (GalT KO) pig into a non-human primate (NHP). Adenosine which is a final metabolite following sequential hydrolysis of nucleotide by ecto-nucleotidases such as CD39 and CD73, act as a regulator of coagulation, and inflammation. Thus xenotransplantation of CD39 and CD73 expressing pig under the GalT KO background could lead to enhanced survival of recipient NHP. We constructed a human CD39 and CD73 expression cassette designed for endothelial cell-specific expression using porcine Icam2 promoter (pIcam2-hCD39/hCD73). We performed isolation of endothelial cells (pAEC) from aorta of 4 week-old GalT KO and membrane cofactor protein expressing pig ($GalT^{-MCP/-MCP}$). We were able to verify that isolated cells were endothelial-like cells using immunofluorescence staining analysis with von Willebrand factor antibody, which is well known as an endothelial maker, and tubal formation assay. To find optimal condition for efficient transfection into pAEC, we performed transfection with GFP expression vector using four programs of nucleofection, M-003, U-023, W-023 and Y-022. We were able find that the program W-023 was optimal for pAEC with regard to viability and transfection efficiency by flow cytometry and fluorescent microscopy analyses. Finally, we were able to obtain $GalT^{-MCP/-MCP}/CD39/CD73$ pAEC expressing CD39 and CD73 at levels of 33.3% and 26.8%, respectively. We suggested that pACE isolated from $GalT^{-MCP/-MCP}$ pig might be provided as a basic resource to understand biochemical and molecular mechanisms of the rejections and as an alternative donor cells to generate $GalT^{-MCP/-MCP}/CD39/CD73$ pig expressing CD39 and CD73 at endothelial cells.

• Journal of Fashion Business
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• v.2 no.2
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• pp.85-96
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• 1998

This is a study on the revitalization of the fashion design studios (planning and production companies of clothing) in compliance with the request from the Small and Medium Business Administration. It's purpose is to strengthen the competence of the petty company and eventually frourish the Korean Fashion Business. The research method to conduct this study consists of two parts. First part is the research on the domestic industry mainly by questionaire, interviews and literature reviews. After making out the directory of the design studios (total of 700), 85 studios were selected for the questionaire. And by interviewing them, data with more depth was obtained. The second part is the research of the foreign cases. In the case of Japan, survey was done on the spot. However, in the case of Italy, literature review and interview with specialists were made. Also in order to higher the efficiency of the study an advisory committee and final explanatory meeting was carried into effect. This study has started first by looking into the significance and function of the fashion design studios. Then, a full scale examination was made centering around the questionarie of the present conditions of the companies. General conditions were first observed. Then the survey was done by grouping the industry by the industrial classifications such as woven, knit, leather mustang fur and fashion accesaries. The problems these companies face are that they are petty in scale and in lack of speciality, professional training, government support. Also, they are without an association or an organization that speaks for them and they face problems while doing business with others. Cases of Japan and Italy were surveyed as the example of the advanced nations. In the case of Japan, development of planning and management company, Fashion Soft House, was analyzed. Italy for instance, the function of the studios was looked into centering around the silk complex, Como, knit product complex, Capri. Also, precedent cases of brands and developing factor of the fashion industry was examined. Finally, on the basis of the result of the study, a plan to revitalize the fashion design studios was presented centering around the strategic planning, management, production, developing of talent, improvement of relationship between business acqaintance, and the support policy of the government.

• Journal of Fashion Business
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• v.14 no.1
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• pp.57-75
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• 2010

This study is done in Shanghai area by sample survey of female college students in their lower 20s. Through direct contact survey, this study collected and analyzed information on figure to understand feature of breasts and measurements of body to provide base information to improve product of brassiere for adult female in China. Data was analyzed by using SPSSWIN 13.0 Program and SAS 9.0. 1. From a result of factor analysis on 40 items of measures to derive the components of the shape of the breast, 6 factors were derived such as the factors to show the obesity of the bust, to show the ratio of the upper and lower area of the lower bust, droop and volume, to show the internal shape and broadness of the bust, to show the location and vertical size of the bust, to show the protrusion of the bust and the eternal shape of the bust. 2. From a result of classification on the shape of the breast of the Chinese women in their early 20s, 4 types were selected. Type 1 is the protrusion type that a woman has a broad drooped breast due to the development in the upper and lower parts of the chest as well as the highest height, a high degree of obesity in the bust part, big volume and much protrusion. Type 2 is the hemisphere type that the degree of obesity in the breast is second to Type 1 and a breast is located at a region higher than Type 1 as the degree of obesity in the breast is secondly highest, the lower part of the bust is bigger than the Type 1 and the degree of being broad and drooped is second to Type 1. Type 3 is the cone type with a breast of being drooped and broad a little bit and a certain degree of a volume. Type 4 is the flat type with the smallest value in the item that shows the obesity of the bust area and with the smallest value in the R$\ddot{o}$hrer index to have the small and slender body type for the bust area, which is somewhat flat due to a low slope at the internal side of the bust.

• Korean journal of food and cookery science
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• v.29 no.4
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• pp.367-376
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• 2013

The objectives of this study are to provide the food market of internet shopping malls with effective marketing data, to provide basic data for the development of related fields of the study, and ultimately to increase the satisfaction of food consumers of internet shopping malls. To achieve the object of this research, a cluster analysis of the research subjects was carried out based on the following 5 factors of food purchasing attribute that had been deduced by a factor analysis by the types of food purchasers: quality characteristics, informativity, convenience, price and diversity. According to the result of the cluster analysis, the research subjects were classified into the 2 clusters of diversity and informativity. The deduced 2 clusters, together with age and occupation among general characteristics, were used as independent variables to find out food purchasing behaviors and satisfaction at internet shopping malls. The results are as follows: Regarding the frequency of food purchasing experiences at internet shopping malls according to occupation, the highest frequency was shown by those involved in service, sales and self-employed businesses; whereas regarding the frequency according to age, those in their 30s and 40s showed the highest frequency. The total amount of money spent on food purchasing for 1 year at internet shopping malls was shown to increase as age increased. The frequency of the purchasing experiences of agricultural products and fish products was shown to be higher as age increased. However, overall purchase satisfaction was highest among those in their 30s, while lowest among those in their 40s. Regarding satisfaction by the types of food purchased via internet shopping malls, satisfaction was relatively higher with common foods and functional foods, while lower with fish products. Taken together, it was concluded that purchasing behaviors at internet food shopping malls, such as the frequency of purchasing experiences and purchase amount, varied depending on age rather than purchasing attribute. Accordingly, in order to vitalize internet food shopping malls, it would be necessary to provide customized food shopping information for individual age groups.

• Food Science of Animal Resources
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• v.40 no.4
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• pp.659-667
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• 2020

Muscle stem cells isolated from domestic animals, including cows and pigs, were recently spotlighted as candidates for the production of alternative protein resources, so-called cultured meat or lab-grown meat. In the present study, we aimed to optimize the in vitro culture conditions for the long-term expansion of pig muscle stem cells via the screening of various signaling molecules. Pig muscle stem cells were collected from the biceps femoris muscles of 3-d-old crossbred pigs (Landrace×Yorkshire×Duroc, LYD) and cultured in minimum essential medium-based growth media. However, the pig muscle stem cells gradually lost their proliferation ability and featured morphologies during the long-term culture over two weeks. To find suitable in vitro culture conditions for an extended period, skeletal muscle growth medium-2, including epidermal growth factor (EGF), dexamethasone, and a p38 inhibitor (SB203580), was used to support the stemness of the pig muscle stem cells. Interestingly, pig muscle stem cells were stably maintained in a long-term culture without loss of the expression of myogenic marker genes as determined by PCR analysis. Immunostaining analysis showed that the stem cells were capable of myogenic differentiation after multiple passaging. Therefore, we found that basal culture conditions containing EGF, dexamethasone, and a p38 inhibitor were suitable for maintaining pig muscle stem cells during expanded culture in vitro. This culture method may be applied for the production of cultured meat and further basic research on muscle development in the pig.

• Journal of the Korean Society for Marine Environment & Energy
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• v.11 no.2
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• pp.98-104
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• 2008

In April, July, October and December, 2003, we investigated the effects of water temperature, salinity and extinction coefficient on the distribution of phytoplankton communities at 22 stations in Jinju Bay of Korea. Water temperature and salinity showed a wide range of $10.4^{\circ}C-21.8^{\circ}C$ and 4.34-33.21 psu. Extinction coefficients showed a range of 0.09-3.08, above 1 from almost all the areas except in some central areas, especially, showed highest value (>2) in the estuary area. In phytoplankton, a total of 95 species belonging to 51 genera were identified. The predominant species were mainly diatoms throughout the year. Dominant species was Thalassionema nitzschioides, Skeletonema costatum, Thalassiosira sp. in April, S. costatum, Leptocylindrus danicus in July, C. debilis, S. costatum, C. curvisetus, Pseudonitzshia pungens in October, S. costatum, Asterionellopsis glacialis and C. debilis in December. S. costatum was a major dominant species for all the seasons. Considering the results of literature which is about physiological study, S. costatum seems to be euryhaline and eurythermal, and high affinity on the irradiance. Thus, the species might have been spread population in Jinju Bay where is characteristic of wide range of water temperature, salinity and high extinction coefficients.