• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2005년도 한국식물병리학회 임시총회 및 춘계학술발표회
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• pp.81.2-81
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• 2005

• 한국표면공학회:학술대회논문집
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• 한국표면공학회 2003년도 추계학술발표회초록집
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• pp.112-113
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• 2003

• 한국작물학회:학술대회논문집
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• 한국작물학회 2019년도 추계학술대회
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• pp.164-164
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• 2019

• Journal of Microbiology and Biotechnology
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• 제17권7호
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• pp.1090-1097
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• 2007

Genomic analysis of Thermococcus sp. NA1 revealed the presence of a 3,927-base-pair (bp) family B-type DNA polymerase gene, TNA1_pol. TNA1_pol, without its intein, was overexpressed in Escherichia coli, purified using metal affinity chromatography, and characterized. TNA1_pol activity was optimal at pH 7.5 and $75^{\circ}C$. TNA1_pol was highly thermostable, with a half-life of 3.5h at $100^{\circ}C$ and 12.5h at $95^{\circ}C$. Polymerase chain reaction parameters of TNA1_pol such as error-rate, processivity, and extension rate were measured in comparison with rTaq, Pfu, and KOD DNA polymerases. TNA1_pol averaged one incorrect bp every 4.45 kilobases (kb), and had a processivity of 150 nucleotides (nt) and an extension rate of 60 bases/s. Thus, TNA1_pol has a much faster elongation rate than Pfu DNA polymerase with 7-fold higher fidelity than that of rTaq.

• 한국진공학회지
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• 제14권3호
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• pp.126-131
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• 2005

소각중성자산란 (Small-angle neutron scattering, SANS)은 물질의 내부 나노구조 분석에 매우 중요한 실험방법론이다. 본 논문에서는 30MW HANARO 연구용 원자로의 냉중성자 연구시설에 설치하기 위하여 한국과학기술원과 한국원자력연구소가 공동으로 개발 중인 40m SANS장치의 개념설계 결과를 보고한다. 장치의 설계 최적화를 위한 전산모사를 수행하였으며, 이를 바탕으로 40m SANS 장치의 주요설계 인자를 결정하였다. 40m SANS 장치의 가능한 Q-range는 $0.0005\;\AA^{-1}-1.0\AA^{-1}$ 이며, 이는 세계 최고 수준의 SANS Q-range에 해당된다. 각 Q-range에 따른 시료위치에서의 중성자 속을 계산하였으며 전산모사에 사용된 냉중성자원이 구현될 시 세계적 수준의 값을 얻을 수 있을 것으로 예상된다.

• Journal of Microbiology and Biotechnology
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• 제24권9호
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• pp.1196-1206
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• 2014

A metagenomic fosmid library was constructed using genomic DNA isolated from the gut microflora of Hermetia illucens, a black soldier fly. A cellulase-positive clone, with the CS10 gene, was identified by extensive Congo-red overlay screenings for cellulase activity from the fosmid library of 92,000 clones. The CS10 gene was composed of a 996 bp DNA sequence encoding the mature protein of 331 amino acids. The deduced amino acids of CS10 showed 72% sequence identity with the glycosyl hydrolase family 5 gene of Dysgonomonas mossii, displaying no significant sequence homology to already known cellulases. The purified CS10 protein presented a single band of cellulase activity with a molecular mass of approximately 40 kDa on the SDS-PAGE gel and zymogram. The purified CS10 protein exhibited optimal activity at $50^{\circ}C$ and pH 7.0, and the thermostability and pH stability of CS10 were preserved at the ranges of $20{\sim}50^{\circ}C$ and pH 4.0~10.0. CS10 exhibited little loss of cellulase activity against various chemical reagents such as 10% polar organic solvents, 1% non-ionic detergents, and 0.5 M denaturing agents. Moreover, the substrate specificity and the product patterns by thin-layer chromatography suggested that CS10 is an endo-${\beta}$-1,4-glucanase. From these biochemical properties of CS10, it is expected that the enzyme has the potential for application in industrial processes.

• Journal of Microbiology and Biotechnology
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• 제24권11호
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• pp.1551-1558
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• 2014

The esterase gene Est8 from the thermophilic bacterium Bacillus sp. K91 was cloned and expressed in Escherichia coli. The monomeric enzyme exhibited a theoretical molecular mass of 24.5 kDa and an optimal activity around $50^{\circ}C$ at pH 9.0. A model of Est8 was constructed using a hypothetical YxiM precursor structure (2O14_A) from Bacillus subtilis as template. The structure showed an ${\alpha}/{\beta}$-hydrolase fold and indicated the presence of a typical catalytic triad consisting of Ser-11, Asp-182, and His-185, which were investigated by site-directed replacements coupled with kinetic characterization. Asp-182 and His-185 residues were more critical than the Ser-11 residue in the catalytic activity of Est8. A comparison of the amino acid sequence showed that Est8 could be grouped into the GDSL family and further classified as an SGNH hydrolase. Est8 is a new member of the SGNH hydrolase subfamily and may employ a different catalytic mechanism.

• 한국엔터테인먼트산업학회논문지
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• 제15권5호
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• pp.85-98
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• 2021

본 논문은 '스타 페르소나(star persona)' 개념 중에서 '캐릭터 배우의 인물창조'에 관한 연구다. 2020년에 이루어진 '퍼스낼리티 배우(personality actor)의 인물창조(characterization)'에 관한 후속 연구다. 국내 배우 중에서 연기 스펙트럼이 넓고 매번 각기 다른 캐릭터 구현력이 뛰어난 캐릭터 배우로서 이성민을 선정했으며, 그의 2018년도 출연작인 영화 '공작'에서의 북한 고위 관료 '리명운' 역할을 토대로 캐릭터 배우의 인물창조 방법에 관해서 논의했다. 스타니슬라브스키는 성격구축, 즉 인물창조를 배우 예술의 최고봉이라고 주장했다. 그러나 이와 같은 성격 구현은 배우들에게 각고의 노력과 헌신을 요하는 작업이다. 배우 이성민은 '리명운'이라는 역할을 연기하기 위해서 북한 사투리를 연습했고, 여기에 엘리트의 말투를 가미했다. 극적 긴장감과 상황의 사실감을 극대화하기 위해서 인물들의 감정과 대사가 중심이 되는 인물 단독 숏 안에서 시선을 고정시키며, 한 호흡으로 대사를 내뱉으면서 극의 긴장감을 고조시키는 연기 방식을 구현했다. 콘티뉴이티와 앵글 또한, 인물의 성격구축과 정서, 감정 등을 표현하는데 매우 중요한 역할을 수행한다. 본 논문에서는 영화 '공작'에서 캐릭터 배우의 '리명운'의 성격구축 방법을 논의해보았다.

• 한국분말야금학회:학술대회논문집
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• 한국분말야금학회 2006년도 Extended Abstracts of 2006 POWDER METALLURGY World Congress Part 1
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• pp.413-414
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• 2006

The effects of several experimental parameters on the formation of stable Ni nanoparticles dispersion were investigated. The suspensions of Ni nanoparticles were produced in organic solvents using Hypermer KD-2 as a dispersant. The transmission profiles, particle size distribution, zeta potential, and visual inspection results were used to discuss the stability of the dispersion. The optimal conditions for the formation of stable dispersion are evaluated.

• Reproductive and Developmental Biology
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• 제34권4호
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• pp.283-288
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• 2010

During normal early embryonic development in mammals, the global pattern of genomic DNA methylation undergoes marked. changes. The level of methylation is high in male and female gametes. Thus, we cloned the cDNA of the porcine DNA methyltransferase 1 (Dnmt1) gene to promote the efficiency of the generation of porcine clones. In this study, porcine Dnmt1 cDNA was sequenced, and Dnmt1 mRNA expression was detected by reverse transcription-polymerase reaction (RT-PCR) in porcine tissues during embryonic development. The porcine Dnmt1 cDNA sequence showed more homology with that of bovine than human, mouse, and rat. The complete sequence of porcine Dnmt1 cDNA was 4,774-bp long and consisted of an open reading frame encoding a protein of 1611 amino acids. The amino acid sequence of porcine DNMT1 showed significant homology with those of bovine (91%), human (88%), rat (76%), and mouse (75%) Dnmt1. The expression of porcine Dnmt1 mRNA was detected during porcine embryogenesis. The mRNA was detected at stages of porcine preimplantation development (1-cell, 2-cell, 4-cell, 8-cell, morula, and blastocyst stages). It was also abundantly expressed in tissues (lung, ovary, kidney and somatic cells). Further investigations are necessary to understand the complex links between methyltransferase 1 and the transcriptional activity in cloned porcine tissues.