• International Journal of Oral Biology
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• 제46권3호
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• pp.140-145
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• 2021

This study aimed to develop Lautropia mirabilis-specific quantitative real-time polymerase chain reaction (qPCR) primers based on the sequence of DNA-directed RNA polymerase subunit beta gene. The PrimerSelect program was used in designing of the qPCR primers, RTLam-F4 and RTLam-R3. The specificity of the qPCR primers were performed by conventional PCR with 37 strains of 37 oral bacterial species, including L. mirabilis. The sensitivity of the primers was determined by qPCR with the serial dilution of purified genomic DNA of L. mirabilis KCOM 3484, ranged from 4 ng to 4 fg. The data showed that the qPCR primers could detect only L. mirabilis strains and as little as 40 fg of genome DNA of L. mirabilis KCOM 3484. These results indicate that this qPCR primer pair (RTLam-F4/RTLam-R3) may be useful for species-specific detection of L. mirabilis in epidemiological studies of oral bacterial infectious diseases such as periodontal disease.

• 한국항만학회지
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• 제11권1호
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• pp.65-73
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• 1997

The objective of this paper is to suggest a decision support system allowing automatic loading and discharging planning for tanker ships, and provide a working prototype. Particular attention is placed on developing a feasible system for improving the speed and accuracy of cargo planning by combining practice and theoretical solution methods. The system is consisted of a simulation model for tank allocation and an expert system for working sequence. Several program tests are undertaken with randomly generated input data. The results suggest that the system is quite meaningful even though there are still some problems to be solved. The paper concludes with a discussion of issues for future development of the decision support system.

• 한국정밀공학회지
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• 제12권8호
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• pp.106-113
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• 1995

Surface mounters are used to mount the parts of electronic products on PCB(Printed Circuit Board. Those machines rely on high precision, high productivity, and software for accomodation of use. In this paper, two algorithms and a software which can give the users the acco-modation and high productivity for rotary type surface mounters are presented. The algo- rithms for development of of the software consider all factors which affect the effeciency of the operation. And the interface between user and machine for the accomodation of use can be achieved through the developed software.

• Animal Bioscience
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• 제36권10호
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• pp.1517-1529
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• 2023

Objective: The objective of this study was to investigate the phylogenetic and expression analysis of the angiopoietin-like (ANGPTL) gene family and their role in lipid metabolism in pigs. Methods: In this study, the amino acid sequence analysis, phylogenetic analysis, and chromosome adjacent gene analysis were performed to identify the ANGPTL gene family in pigs. According to the body weight data from 60 Jinhua pigs, different tissues of 6 pigs with average body weight were used to determine the expression profile of ANGPTL1-8. The ileum, subcutaneous fat, and liver of 8 pigs with distinct fatness were selected to analyze the gene expression of ANGPTL3, ANGPTL4, and ANGPTL8. Results: The sequence length of ANGPTLs in pigs was between 1,186 and 1,991 bp, and the pig ANGPTL family members shared common features with human homologous genes, including the high similarity of the amino acid sequence and chromosome flanking genes. Amino acid sequence analysis showed that ANGPTL1-7 had a highly conserved domain except for ANGPTL8. Phylogenetic analysis showed that each ANGPTL homologous gene shared a common origin. Quantitative reverse-transcription polymerase chain reaction analysis showed that ANGPTL family members had different expression patterns in different tissues. ANGPTL3 and ANGPTL8 were mainly expressed in the liver, while ANGPTL4 was expressed in many other tissues, such as the intestine and subcutaneous fat. The expression levels of ANGPTL3 in the liver and ANGPTL4 in the liver, intestine and subcutaneous fat of Jinhua pigs with low propensity for adipogenesis were significantly higher than those of high propensity for adipogenesis. Conclusion: These results increase our knowledge about the biological role of the ANGPTL family in this important economic species, it will also help to better understand the role of ANGPTL3, ANGPTL4, and ANGPTL8 in lipid metabolism of pigs, and provide innovative ideas for developing strategies to improve meat quality of pigs.

• 한국통신학회논문지
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• 제24권8A호
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• pp.1149-1155
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• 1999

저궤도위성 시스템은 지구 및 과학 관측분야(지도제작, 해양생태감시, 우주환경 관측, 기상관측, 대기관측 등)와 군사분야(군사통신, 첩보, 적군정찰 등)에서 사용되었으며, 최근에는 상업적 이용을 위해 GMPCS를 위한 이동위성통신 분야에서 개발이 진행되고 있다. 우리나라에서는 1999년 10월 발사를 목표로 다목적 실용위성 1호가 개발되고 있다. 본 논문에서는 저궤도위성 관제시스템을 위한 위성링크를 설계하고 위성의 움직임에 따른 위성의 통과시간과 지구국의 양각 관계를 계산한다. 그리고 수신데이터의 패킷오율을 확률이론에 의해 유도하고, RT모드와 PB모드 그리고 RT+RNG모드에 대한 패킷오율을 위성과 통과시간에 따라 예측한다. 패킷오율 계산결과는 RT모드, PB모드, RT+RNG모드의 순으로 좋으며 EFP의 평균도 각각 99.999999%, 99.995945%로 계산되었다. 따라서 원격측정 데이터의 전송순서는 위성의 통과시간에 따른 PER 순서로 즉, RT, PB, RT+RNG순으로 결정한다.

• 한국동물생명공학회지
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• 제37권4호
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• pp.292-297
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• 2022

Direct injection of CRISPR/Cas9 into zygotes enables the production of genetically modified nonhuman primates (NHPs) essential for modeling specific human diseases, such as Usher syndrome, and for developing novel therapeutic strategies. Usher syndrome is a rare genetic disease that causes loss of hearing, retinal degeneration, and problems with balance, and is attributed to a mutation in MYO7A, a gene that encodes an uncommon myosin motor protein expressed in the inner ear and retinal photoreceptors. To produce an Usher syndrome type 1B (USH1B) rhesus macaque model, we disrupted the MYO7A gene in developing zygotes. Identification of appropriately edited MYO7A embryos for knockout embryo transfer requires sequence analysis of material recovered from a trophectoderm (TE) cell biopsy. However, the TE biopsy procedure is labor intensive and could adversely impact embryo development. Recent studies have reported using cell-free DNA (cfDNA) from embryo culture media to detect aneuploid embryos in human in vitro fertilization (IVF) clinics. The cfDNA is released from the embryo during cell division or cell death, suggesting that cfDNA may be a viable resource for sequence analysis. Moreover, cfDNA collection is not invasive to the embryo and does not require special tools or expertise. We hypothesized that selection of appropriate edited embryos could be performed by analyzing cfDNA for MYO7A editing in embryo culture medium, and that this method would be advantageous for the subsequent generation of genetically modified NHPs. The purpose of this experiment is to determine whether cfDNA can be used to identify the target gene mutation of CRISPR/Cas9 injected embryos. In this study, we were able to obtain and utilize cfDNA to confirm the mutagenesis of MYO7A, but the method will require further optimization to obtain better accuracy before it can replace the TE biopsy approach.

• 한국발생생물학회지:발생과생식
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• 제16권4호
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• pp.301-307
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• 2012

Olive flounder Paralichthys olivaceus is one of the most widely cultured fish species in Korea. Although olive flounder receive attention from aquaculture and fisheries and extensive research has been conducted eye morphological change in metamorphosis, but little information was known to molecular mechanism and gene expression of eye development- related genes during the early part of eye formation period. For the reason of eyesight is the most important sense in flounder larvae to search prey, the screening and identification of expressed genes in the eye will provide useful insight into the molecular regulation mechanism of eye development in olive flounder. Through the search of an olive flounder DNA database of expressed sequence tags (EST), we found a partial sequence that was similar to crystallin beta A1 and gamma S. Microscopic observation of retinal formation correspond with the time of expression of the crystallin beta A1 and gamma S gene in the developmental stage, these result suggesting that beta A1 and gamma S play a vital role in the remodeling of the retina during eye development. The expression of crystallin beta A1 and gamma S were obviously strong in eye at all tested developing stage, it is also hypothesized that crystallin acts as a molecular chaperone to prevent protein aggregation during maturation and aging in the eye.

• 대한수학교육학회지:수학교육학연구
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• 제18권3호
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• pp.373-389
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• 2008

이 연구에서는 예비중등교사들의 수학화 학습을 위해 분할모델과 일반화된 피보나치 수열 사이의 관계를 탐구하는 교수단원을 설계한다. 이 교수단원에서는 먼저 예비중등교사들이 조직해야 할 현상을 탐구문제의 형태로 제공한다. 그들은 이 탐구문제를 해결하면서, 그것을 조직하는 본질 즉, 분할의 수에 대한 패턴을 찾게 된다. 이 과정에서 점차 커지는 분할될 수의 집합에 따라 분할모델의 유형도 다양해진다. 이러한 분할모델에 대한 분할의 수를 구하고, 이 수들 사이의 패턴을 찾아 공식을 만들고, 이 공식들이 일반화된 피보나치 수열과 관계가 있음을 찾는다. 분할모델과 피보나치 수열 사이의 이러한 관계는 이전에 알려지지 않은 소재인 만큼, 그것은 예비중등교사들로 하여금 수학화를 가상적으로 연습하게 하는 것이 아니라, 실제처럼 연습할 수 있게 된다.

• 한국미생물·생명공학회지
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• 제31권2호
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• pp.124-128
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• 2003

대전광역시 근교의 야산과 들판 등지에서 썩은 나뭇잎, 짚, 흙을 채취하여 각각을 배양한 다음 Congo red test에 의해 cellulase 활성을 보이는 균주를 선별하였다. Genomic DNA를 분리한 후 PCR을 수행하여 DNA sequence를 Gene Bank를 통해 분석한 결과 A. tubingensis로 밝혀졌다. 이것을 배양하여 상등액을 crude enzyme으로 사용하여 온도와 pH를 달리하면서 효소의 활성정도를 측정하였다. 대조균주로 A. oryzae KCTC 6291를 이용하였고, 본 연구를 통하여 분리한 균주인 A. tubingensis가 생산하는 cellulase는 A. oryzae의 cellulase에 비하여 각각 다른 온도와 pH에서 높은 안정성을 보여주었다. A. tubingensis는 각각의 온도에서 활성의 정도가 비슷했으며, 45$^{\circ}C$, 55$^{\circ}C$에서 높은 활성을 나타내고 있지만, 고르게 활성이 나타났다. 또한 pH 12.0에서 가장 높은 활성을 보여 주었고, pH 2.0, 3.0, 4.0에서는 양쪽 모두 거의 활성이 없었으며, 중성, 염기성에 대해서 활성에는 큰 변화가 없었다. 따라서, 분리 동정한 A. tubingensis는 온도와 pH에서 고르게 활성을 나타내므로 생균제로 활용할 수 있는 범위가 클 것으로 여겨진다.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2000년도 추계학술대회 논문집
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• pp.591-594
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• 2000

This paper describes a research work of developing a computer-aided design of product with bending and piercing for progressive working. An approach to the system for progressive working is based on the knowledge-based rules. Knowledge for the system is formulated from plasticity theories, experimental results and the empirical knowledge of field experts. The system has been written in AutoLISP on the AutoCAD with a personal computer and is composed of four main modules, which are input and shape treatment, flat pattern layout, strip layout and die layout module. Based on knowledge-based rules, the system is designed by considering several factors such as radius and angle of bend, material and thickness of product, complexities of blank geometry and punch profile, bending sequence, and availability of press. Strip layout drawing generated by the piercing processes with punch profiles divided into for external area is simulated in 3-D graphic forms, including bending sequences for the product with piercing and bending. Results obtained using the modules enable the manufacturer for progressive working of electronic products to be more efficient in this field.