• Journal of Forest and Environmental Science
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• v.24 no.3
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• pp.155-158
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• 2008

Site index is the essential tool for forest management to estimate the productivity of forest land Generally, site index equation is developed and used by relationship between stand age and dominant tree heights. However, there is a limit to use the site index equation in the application of variable ages, environmental influence, and estimation of site index for unstocked land. Therefore, it was attempted to develop a new site index equations based on various environmental factors including site and topographical variables. This study was conducted to develop regional site index equations based on the relationship between site index and soil factors for Pinus densiflora. Environmental factors that obtained from GIS application, were selected by stepwise-regression. Site index Equation was estimated by multiple regression from selected factors. Four environmental factors were selected in the final site index equations by stepwise regression. It was observed that coefficients of determination for site index equations were ranged from 0.34 which seem to be relatively low but good enough for estimation of forest stand productivity. The site index equations developed in this study were also verified to be useful by three evaluation statistics such as model's estimation bias, model's precision and mean square error type of measure.

• Journal of Environmental Science International
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• v.22 no.5
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• pp.555-562
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• 2013

Jeju Island, the heaviest raining area in Korea, is a volcanic Island located at the southernmost of Korea, but most streams are of the dry due to its hydrological/geological characteristics different from those of inland areas. Therefore, there are limitations in applying the results from the mainland to the studies on stream run-off characteristics analysis and water resource analysis of Jeju Island. In this study, the SWAT(soil & water assessment tool) model is used for the Hwabuk stream watershed located east of the downtown to calculate the long-term stream run-off rate, and WMS(watershed modeling system) and HEC-HMS(hydrologic modeling system) models are used to figure out the stream run-off characteristics due to short-term heavy rainfall. As the result of SWAT modelling for the long-term rainfall-runoff model for Hwabuk stream watershed in 2008, 5.66% of the average precipitation of the entire basin was run off, with 3.47% in 2009, 8.12% in 2010, and root mean square error(RMSE) and determination coefficient($R^2$) was 496.9 and 0.87, respectively, with model efficient(ME) of 0.72. From the results of WMS and HEC-HMS models which are short-term rainfall-runoff models, unless there was a preceding rainfall, the runoff occurred only for rainfall of 40mm or greater, and the run-off duration averaged 10~14 hours.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.9
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• pp.1227-1231
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• 2002

A powerful tool for chicken transgenesis could be established by employing a germline chimera production through primordial germ cell transplantation. This study was conducted to examine whether foreign gene-transfected gonadal primordial germ cells (gPGCs) have a migration activity into the gonad after transfer to recipient embryos. In Experiment 1, gPGCs of Korean Ogol Chicken were retrieved from 5.5-day-old embryos and subsequently transferred to the dorsal aorta of 2.5-day-old White Leghorn embryos after being labeled with PKH26 fluorescent dye. To confirm migration activity after transplantation, recipient embryos were sacrificed and examined on 3 days after transfer. Sex determination was concomitantly undertaken to examine whether sex of recipient embryos could affect the migration activity of gPGCs. All of embryonic gonads examined showed positive signals with PKH26 fluorescence and W-chromosome specific band by polymerase chain reaction (PCR) was detected in male embryos when gPGCs with ZW chromosome were transferred to recipient embryos. In Experiment 2, retrieved gPGCs were transfected with LacZ gene-containing cytomegalovirus promoter ($pCMV{\beta}$) by electroporation and subsequently transferred to recipient embryos. LacZ gene expression was identified in the gonads of 6 or 10-day-old recipient embryos and hatched-chicks. A total of 20 embryos and 12 hatched-chicks were examined and 11 of them (10 embryos and one hatched chicken; 11/32=34.4%) expressed $\beta$-galactosidase, a marker substance of LacZ gene. The results of this study demonstrated that foreign gene-transfected gPGCs can migrate and settle down into the gonad after being transferred into the blood vessel of the recipient embryos. This established technique will contribute to developing a peer biotechnology for transgenic chicken.

• Korean Journal of Computational Design and Engineering
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• v.20 no.4
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• pp.348-356
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• 2015

It is important to devise methods for assisting dentists to consistently determine implant positions and directions and to accurately perform drilling tasks during dental implant surgery. In this paper, we propose a novel approach to tooth implant placement planning which deals with the determination of the positions and directions of multiple implant fixtures for a set of missing mandibular teeth and suggests the selection of the sizes and types of the implant fixtures. We combine Korean standard data in the sizes and positions of human teeth with the patient specific 3D models of mandibular jawbones, nerve curves, and neighboring teeth around the missing teeth in order to determine the positions and directions of the implant fixtures for the missing teeth. Using the geometric and spatial information of the jawbones, the teeth and the implant fixtures, we can construct the 3D models of surgical guide stents which are crucial to perform drilling tasks with ease and accuracy. Adopted in 3D simulation of dental implant placement, the approach can provide surgeon students with good educational contents. We also expect that, with further work, the approach can be used as a useful tool to plan for dental implant surgery.

• IMMUNE NETWORK
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• v.8 no.3
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• pp.82-89
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• 2008

Background: Although a skin test is the primary option for detecting allergen-specific IgE in clinics, the serum IgE immunoassay is also important because it allows for the diagnosis of allergy without any accompanying adverse effect on the patient. However, the low detection limit of IgE levels by immunoassay may restrict the use of the method in some occasions, and improving its sensitivity would thus have a significant implication in allergy-immunology clinics. Methods: In this study, we attempted to detect specific serum IgE by using immuno-polymerase chain reaction (IPCR) which combines the antigen-antibody specificity of enzyme-linked immunosorbent assays (ELISAs) with the amplification power of PCR. Results: Our results demonstrated that Blo t5-specific serum IgE can be detected by IPCR with a 100-fold higher sensitivity than ELISA, and cross-reactivity of serum IgE to other mite allergens is able to be analyzed by using only $0.3{\mu}l$ of serum sample. Use of real-time IPCR seemed to permit more convenient determination of specific serum IgE as well. Conclusion: We believe that IPCR can serve as a valuable tool in determining specific serum IgE, especially when the amount of serum sample is limited.

• Journal of Microbiology and Biotechnology
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• v.19 no.1
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• pp.83-92
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• 2009

Individual immunochromatographic assays (ICG) for ochratoxin A (OTA) and zearalenone (ZEA) were optimized and used in the development of a one-step simultaneous immunochromatographic assay (OS-ICG) for the rapid multianalysis of two mycotoxins in corn samples. The nitrocellulose membrane of the OS-ICG was treated with OTA-bovine serum albumin (BSA), ZEA-ovalbumin (OVA), and anti-mouse IgG in the OTA test, ZEA test, and control zones, respectively. Monoclonal antibody-gold conjugates (OTA3 MAb-gold and ZEA2C5 MAb-gold) were sprayed onto the conjugate pad. The visual detection limits were 2.5 and 5 ng/ml for OTA and ZEA, respectively, and the results were obtained within 15 min after starting the analysis. An efficient, simple, and rapid extraction method using 30% MeOH/PBS was established and validated by analyzing the corn samples spiked with OTA/ZEA mixtures (0/0, 5/10, 10/20, and $20/30\;{\mu}g/kg$). The cut-off values of the OS-ICG for the spiked corn were 5 and $10\;{\mu}g/kg$ for OTA and ZEA, respectively. Natural corn samples were analyzed by OS-ICG, direct competitive enzyme-linked immunosorbent assay (DC-ELISA), and HPLC. Results of the OS-ICG were in good agreement with those obtained by DC-ELISA and HPLC. The developed OS-ICG offers a rapid, easy-to-use, and portable analytical system and can be used as a convenient qualitative tool for the on-site simultaneous determination of OTA and ZEA in cereals, food, and agricultural products in one analytical cycle.

• Journal of Biosystems Engineering
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• v.39 no.3
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• pp.244-252
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• 2014

Purpose: The development of an efficient in vitro cell culture device to process various cells would represent a major milestone in biological science and engineering. However, the current conventional macro-scale in vitro cell culture platforms are limited in their capacity for detailed analysis and determination of cellular behavior in complex environments. This paper describes a microfluidic-based culture device that allows accurate control of parameters of physical cues such as pressure. Methods: A microfluidic device, as a model microbioreactor, was designed and fabricated to culture Saccharomyces cerevisiae and Chlamydomonas reinhardtii under various conditions of physical pressure stimulus. This device was compatible with live-cell imaging and allowed quantitative analysis of physical cue-induced behavior in yeast and microalgae. Results: A simple microfluidic-based in vitro cell culture device containing a cell culture channel and an air channel was developed to investigate physical pressure stress-induced behavior in yeasts and microalgae. The shapes of Saccharomyces cerevisiae and Chlamydomonas reinhardtii could be controlled under compressive stress. The lipid production by Chlamydomonas reinhardtii was significantly enhanced by compressive stress in the microfluidic device when compared to cells cultured without compressive stress. Conclusions: This microfluidic-based in vitro cell culture device can be used as a tool for quantitative analysis of cellular behavior under complex physical and chemical conditions.

• Journal of the Korean Regional Science Association
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• v.14 no.1
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• pp.91-107
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• 1998

Waste disposal problem is one of the most important social welfare indicators in urban area, because the volume of waste generated from urban area is remarkable. So far most waste of Seoul has been disposed at landfills. However, this landifill disposal method is confronted with several difficulties in recent. As public concern on environmental problem increases and autonomy system is settled down, local community people of the landfills refuse to receive the waste produced other places any more. It brought reginal confliction problem between waste sending and receiving by refusing to accept waste from certain region. Furthermore, it is difficult to find another place to fill up the waste, while the existing landfiis is reached at the limit in the near future. In terms of environmental aspects landfill method is not the best way to dispose waste. It contaminates the soil and ground/underground water by leaking water containing many serious pollutants as well as offensive oder. In terms of equity, this waste disposal method is not fair. Environmental pollution causes damage to residence near to the landfills, while the waste produced other places. In order to satisfy the equity aspect, the waste generated a region should be disposed within the region. Incineration of Waste has been provided as the alternative. Government plans to construct waste incinerator in every anatomy, so the waste produced by local community is disposed within their local autonomous area. However, the location decision is not easy, since waste incinerator is one of the facilities to the community people. We can not apply the existing location models for this problem, because they show strong NIMBY phenomenon for the location. The location of waste incinerator should be determined very carefully with consideration of various location factors and criteria. This study proposes a methodology for determining the location of the waste incinerators by utilizing GIS, which is a power research tool for location decision where various geographical factors related. We drive the location factors which should be considered in the determination of waste incinerators. They involve environmental, socio-economic, and institutional factors. In first, we eliminate the area which is located within the environmental location criteria such as slope, fault line, distance to river, and then eliminate ares which is conflict with the social and institutional criteria.

• Journal of Environmental Impact Assessment
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• v.20 no.5
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• pp.705-716
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• 2011

For the efficient discovery of knowledge and information from the observed systems, data mining techniques can be an useful tool for the prediction of water quality at intake station in rivers. Deterioration of water quality can be caused at intake station in dry season due to insufficient flow. This demands additional outflow from dam since some extent of deterioration can be attenuated by dam reservoir operation to control outflow considering predicted water quality. A seasonal occurrence of high ammonia nitrogen ($NH_3$-N) concentrations has hampered chemical treatment processes of a water plant in Geum river. Monthly flow allocation from upstream dam is important for downstream $NH_3$-N control. In this study, prediction models of water quality based on multiple regression (MR), artificial neural network and data mining methods were developed to understand water quality variation and to support dam operations through providing predicted $NH_3$-N concentrations at intake station. The models were calibrated with eight years of monthly data and verified with another two years of independent data. In those models, the $NH_3$-N concentration for next time step is dependent on dam outflow, river water quality such as alkalinity, temperature, and $NH_3$-N of previous time step. The model performances are compared and evaluated by error analysis and statistical characteristics like correlation and determination coefficients between the observed and the predicted water quality. It is expected that these data mining techniques can present more efficient data-driven tools in modelling stage and it is found that those models can be applied well to predict water quality in stream river systems.

• Journal of Trauma and Injury
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• v.27 no.4
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• pp.139-144
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• 2014

Purpose: Detection and determination of tendon injury in the finger or hand is not easy. Therefore, we aimed to study and evaluate the accuracy and the effectiveness of ultrasonography for the diagnosis of finger tendon injury. Methods: In this study, we enrolled patients, regardless of age and sex, with lacerations on their fingers. Patients with invisible wounds were excluded. We evaluated the accuracy and the effectiveness of ultrasonography and compared the results obtained from ultrasonography and with those obtained by visual observation of the injuries. Results: The sensitivity, the specificity and the accuracy of ultrasonography were found to be 66.7%, 100% and 91.3%, respectively (p<0.001) while those of physical examination were 71.4%, 98.3% and 91.3%, respectively. Small differences were observed between the sensitivities and specificities of the two examinations; however, the accuracies were the same (p<0.001). The area under the receiver operating characteristic (ROC) curve, which was used for diagnosis of tendon rupture using ultrasonography, was found to be 0.985 (95% confidence interval CI: 0.929-0.999),while that of physical examination was 0.938 (95% CI: 0.861-0.980). Conclusion: Ultrasonography can be used an effective diagnostic tool for patients with finger tendon injury.