• YAKHAK HOEJI
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• v.36 no.2
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• pp.129-136
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• 1992

The effects of ginseng saponins, gypsophila saponin, sodium dodecyl sulfate(SDS), and Triton X-100 on membrane $K^+-dependent$ phosphatase activity which is lipid dependent and represents dephosphorylation step of the complete Na+, $K^+-ATPase$ reaction were investigated in this study to elucidate whether the effects of ginseng saponins are due to the detergent action, using sarcolemma enriched preparation isolated from dog ventricle. $Na^+$, $K^+-ATPase$ and $K^+-dependent$ phosphatase activities of cardiac sarcolemma were about $143\;{\mu}mol$ Pi/mg protein/hr and $34\;{\mu}mol$ p-nitrophenol/mg protein/hr, respectively. While ginseng saponins (triol>total>diol) inhibited $K^+-dependent$ phosphatase activity, gypsophila saponin, and low dose of SDS($0.4\;{\mu}g/{\mu}g$ protein), and Triton X-100 ($0.6\;{\mu}g/{\mu}g$ protein) increased the enzyme activity, indicating disruptive effect of detergents on membrane barriers. The activating effect of low doses of Triton X-100 on membrane $K^+-dependent$ phosphatase appeared at concentration decreasing light scattering. However, the inhibitory effect of ginseng saponin appeared before a decrease in light scattering. These results suggest that low concentrations of ginseng saponins inhibit the membrane $K^+-dependent$ phosphatase by interacting directly with enzyme before membrane disruption.

• YAKHAK HOEJI
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• v.44 no.5
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• pp.416-423
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• 2000

There is a growing concern that a wide variety of chemicals released into the environment can disrupt the endocrine system of fish, wildlife and humans. Endocrine disrupting chemicals (EDCs) include pesticides such as DDT lindane and atrazine, the food packaging chemicals, phthalates and bisphenol A, alkylphenol ethoxylate detergents and the chemical industry by-products, dioxins. Xenoestrogens in the environment have been argued about health risk, because of estrogen mimetic chemicals are exposed only small amounts to human. A number of in vivo and in vitro assays are now in use to assess the activity of xenoestrogens in the environment. A human breast cancer cell line (MCF-7) was used to develop in vitro screening assay for the detection of xenoestrogenic environmental pollutants. The E-SCREEN (MCF7-BUS) assay is proposed as a reliable, easy and rapid-to-perform method. To optimize and validate this method before it can be used routinely, several phenol compounds and pesticides suspected to be estrogenic were tested using I-SCREEN assay. The results showed that this method is a valuable tool for screening potential estrogen-mimicking environmental pollutants and quantitative determination of estrogeniciy.

• Fashion & Textile Research Journal
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• v.21 no.2
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• pp.237-243
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• 2019

This study analyzed satisfaction with and washing of newborn clothing (especially the innerwear washing behaviors) which are considered to be the most basic items among newborn clothing.The survey was conducted on mothers with children under 24 months old. Using an average of 6.7 innerwear items that were changed average 2.3 times a day with baby formula and breast milk representing the main contaminants. When washing clothes for a newborn, respondents were mainly concerned with: rinsing residual detergent, removing stains and contaminants, and sterilization and disinfection. Items were washed by a laundry label to prevent clothes damage. A baby-friendly or environmentally-friendly detergent was used to wash before being worn after purchase was separated and washed in various washing courses of the washing machine every day, boiled, rinsed an average 3.6 times, and dried mainly in the sun. Environmentally friendly cotton (59.5%) and organic cotton (41.6%) products were mainly used; however, items were washed separately from regular laundry. Respondents were satisfied with methods of washing. Consumers are concerned about washing because they think that washing affects a newborn's health. Therefore, it is necessary to provide consumers with appropriate information on washing and establish regulations for the use of harmful substances in newborn clothing and detergents.

• Environmental Engineering Research
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• v.24 no.1
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• pp.137-143
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• 2019

In this study, extraction, clean-up and instrumental analytical conditions were optimized for identifying and quantifying methyl-, chloromethylbenzi-, octyl- isothiazolinone (MIT, CMIT, BIT, OIT) and 3-iodo-2-propynyl butyl carbamate (IPBC), widely used biocidal active ingredients in human hygiene consumer products. Accuracy of the developed ultrasonic assisted extraction method followed by HPLC/MS analysis for four isothiazolinones and IPBC ranged between 60.4% and 113% in various types of consumer product samples. Method detection limits ranged 0.011-0.034 mg/kg for wet wipes, 0.57-1.5 mg/kg for liquid detergent and 0.58-1.6 mg/kg for powder detergent. Wet wipes, powder and liquid detergents collected from local market in Korea were analyzed to demonstrate the applicability of the developed method. Even after the regulation of those compounds in wet wipes, CMIT, MIT and IPBC were still frequently detected from complementary wet wipes without brand labels which were distributed to customers at local retail shops and restaurants. The maximum observed concentrations of MIT and CMIT found in those complementary wet wipes were 70.2 mg/kg and 11.3 mg/kg, respectively.

• Journal of Applied Biological Chemistry
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• v.62 no.1
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• pp.51-56
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• 2019

Biofilms facilitate communication among microorganisms for nutrients and protect them from predators and harmful chemicals such as antibiotics and detergents. Biofilms can also act as cores for the development of clogs in many agricultural irrigation systems and in porous media. In this study, we deployed glass units at a depth of 20 m below the ground surface in the groundwater-surface water mixing zone, and retrieved them after 4 months to investigate the potential colonization of indigenous microbial community and possible mineral-microbe assemblages. We observed the periodic formation of microbial colonies by fluorescence dye staining and microscopy, and analyzed the composition of the microbial community in both the mineral-microbe aggregates and groundwater, by next generation sequencing of the 16S rRNA gene amplicons using MiSeq platform. During the course of incubation, we observed an increase in both the mineral-microbe aggregates and content of extracellular polymeric substances. Interestingly, the microbial community from the aggregates featured a high abundance of iron redox-related microorganisms such as Geobacter sp., Comamonadaceae sp., and Burkholderiales incertae sedis. Therefore, these microorganisms can potentially produce iron-minerals within the sediment-microbe-associated aggregates, and induce biofilm formation within the groundwater borehole and porous media.

• Journal of Soil and Groundwater Environment
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• v.25 no.4
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• pp.28-34
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• 2020

Nitrate released from chemical fertilizer, animal wastes, and synthetic detergents can cause methemoglobinemia to infants, thus the standard in drinking water is set to 10 mg/L as World Health Organization recommended. In this study, zero-valent iron-modified rice straw biochar was used to reduce and remove nitrate in the aqueous phase. The rice straw biochar was prepared by pyrolyzing the biomass at 700℃ for 3 hours, and the biochar was modified using 1 M Fe(III), and the Fe(III) on the biochar was reduced to zero-valent iron using sodium borohydride. The modified biochar removed nitrate effectively, which removed more than 91% of nitrate. For the synthetic groundwater, the nitrate removal was lowered to 82% due to the presence of other anions.

• Journal of Animal Reproduction and Biotechnology
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• v.36 no.4
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• pp.253-260
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• 2021

Organ transplantation is currently the most fundamental treatment for organ failure, but there is a shortage of organ supply compared to those in need. Regenerative medicine has recently developed a decellularization technique that overcomes the limitations of conventional organ transplantation and attempts to reconstruct damaged tissues or organs to their normal state. Several decellularization methods have been suggested. In this experiment, the decellularization methods were used to find effective decellularization methods for humanlike porcine placenta. The optimal conditions for decellular support are low DNA content and high glycos amino glycans (GAGs) and collagen content. In order to satisfy this condition, SDS and Triton X-100 and SDS + Triton X-100 were used as the detergent used for decellularization in this experiment. The contents were compared according to the decellularization time (0, 12, 24, 48 and 72 hours), and the concentrations of SDS (0.2, 0.5, 0.7 and 1.0%) were mixed in 1.0% Triton X-100 to analyze the contents. When decellularized using SDS and Triton X-100, respectively, it was confirmed that the contents of DNA and GAGs were opposite to each other. And decellularization treatment for 24 hours at 0.5% SDS was able to obtain an effective decellular support. If decellularization studies of various detergents can be obtained an effective decellular support, and furthermore, cell culture experiments can confirm the effect on the cells.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.28 no.2
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• pp.127-132
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• 2022

The growth of e-commerce market requires more delivery packagings, which protect goods from the damage factors on the delivery. In this study, we have analyzed the distribution environment data (vibration and impact) measured in previous study and compared with global standards (ISTA, ASTM), so that we developed the testing method for Field-to-Lab simulation suitable to the domestic delivery. In order to verify the efficacy of this method, we took the Field-to-Lab tests for 3 packaged products (detergents set, glass tableware set, small furniture), which has been frequent breakages or damages on the actual delivery by e-commerce order, so that we could find out the test results were similar to those of the actual delivery occasions. In addition, we could perform redesign and improvement of the parcel delivery packagings for safe transportation by taking this Field-to-Lab test repeatedly. This test methods was finally proposed to be Korean industrial standard (KS), and is expected to be useful as a designing tool for the packaging optimization between protecting goods and reducing packaging waste.

• Journal of Korean Public Health Nursing
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• v.23 no.2
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• pp.207-218
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• 2009

Purpose: In an attempt to develop a parental health improvement education program for day care children, survey questions were devised to measured the extent of parental health knowledge, health-guidance execution and needs. Method: Participants were 727 parents whose children attended daycare centers in J province informed consent was provided prior to participation. Result: Respondents were overwhelmingly (91.6%) mothers. Questions with high percentage of incorrect answers were "What should I do if my child swallows harmful drugs, bleach or detergents?" (98.1%) and "What should I do if insects or other bugs enter my child's ear?" (96.6%). In most cases, parental health-guidance execution exceeded 90% except in response to the survey question "Does your child have a dental check-up every 6 months" (51%). Taken together, parental education is necessary concerning growth and development (12.8%), nutrition (12.1%), healthy life styles (10.3%) and general health issues (10.0%). Conclusions: Parental health knowledge about preschooler's health varies with degree of income, education and type of employment. Consideration of these variables is important in a parental health education program.

• Korean Journal of Environmental Biology
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• v.33 no.4
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• pp.361-374
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• 2015

Endocirne disruptors (EDs) can cause fertility decrease, developmental disorder, and even cancer in animals. Until 90's, EDs were used in various synthetic products including paints, coatings, detergents, plastics, and plasticizers. Currently, in several countries, the production, trade and use of EDs or EDs-suspected chemicals have been regulated while activity to screen the alternatives for EDs including bisphenol-A, phthalate and nonylphenol is active. Although various toxicity test method was developed and applied for screening of alternatives, however, the safety of alternatives has been not fully demonstrated. Some alternatives have high structural similarity with existing EDs, raising the possible risk of endocrine disruption by alternatives. In an effort to develop the safe alternatives, we reviewed the effects of EDs such as bisphenol-A, phthalates, nonylphenol and their substituents. In addition, in-silico analysis for endocrine disrupting activities of some alternatives was presented.