• Journal of The Korean Society of Inherited Metabolic disease
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• v.6 no.1
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• pp.40-51
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• 2006

Purpose: A personal computer-based system was developed for automated metabolic profiling of organic aciduria and aminoacidopathy by gas chromatography-mass spectrometry and data interpretation for the diagnosis of metabolic disorders Methods: For automatic data profiling and interpretation, we compiled retention time, two target ions and their intensity ratio for 77 organic acids and 13 amino acids metabolites. Metabolites above the cut-off values were flagged as abnormal compounds. The data interpretation was a based on combination of flagged metabolites. Diagnostic or index metabolites were categorized into three groups, "and", "or" and "NO" compiled for each disorder to improve the specificity of the diagnosis. Groups "and" and "or" comprised essential and optional compounds, respectively, to reach a specific diagnosis. Group "NO" comprised metabolites that must be absent to make a definite diagnosis. We tested this system by analyzing patients with confirmed Propionic aciduria and others. Results: In all cases, the diagnostic metabolites were identified and correct diagnosis was founded to be made among the possible disease suggested by the system. Conclusion: The study showed that the developed method could be the method of choices in rapid, sensitive and simultaneous screening for organic aciduria and amino acidopathy with this simplified automated system.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.19 no.4 s.74
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• pp.441-447
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• 2006

This paper deals with the application of the numerical differentiation in the structural analyses. Derivative values of the geometry of structure are definitely needed for analysing the structural behavior. In this study, free vibration problems of arches are chosen for verifying the numerical differential technique in the structural analyses. The curvature parameters composed with the derivatives of arch geometry obtained herein are quite agreed with those of analytical method. Also, natural frequencies with curvature parameters obtained by using the forward fifth polynomial method are quite agreed with those in the literature. The numerical differentiation technique can be practically utilized in the structural analyses.

• Bulletin of the Korean Chemical Society
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• v.29 no.2
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• pp.363-371
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• 2008

As a computational method for the discovery of the effective agonists for PPARd, we address the usefulness of molecular dynamics free energy (MDFE) simulation with explicit solvent in terms of the accuracy and the computing cost. For this purpose, we establish an efficient computational protocol of thermodynamic integration (TI) that is superior to free energy perturbation (FEP) method in parallel computing environment. Using this protocol, the relative binding affinities of GW501516 and its derivatives for PPARd are calculated. The accuracy of our protocol was evaluated in two steps. First, we devise a thermodynamic cycle to calculate the absolute and relative hydration free energies of test molecules. This allows a self-consistent check for the accuracy of the calculation protocol. Second, the calculated relative binding affinities of the selected ligands are compared with experimental IC50 values. The average deviation of the calculated binding free energies from the experimental results amounts at the most to 1 kcal/mol. The computational efficiency of current protocol is also assessed by comparing its execution times with those of the sequential version of the TI protocol. The results show that the calculation can be accelerated by 4 times when compared to the sequential run. Based on the calculations with the parallel computational protocol, a new potential agonist of GW501516 derivative is proposed.

• Archives of Pharmacal Research
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• v.29 no.2
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• pp.135-139
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• 2006

Activity-guided fractionation of the ethanol extract of the whole plant from Solanum lyratum resulted in the isolation of a new pregnane derivative glycoside, 16-dehydropregnenolone 3-O-${\alpha}$-L-rhamnopyranosyl-($1{\to}2$)-${\beta}$-D-glucopyranosid uronic acid (2), as well as other six known compounds: 16-dehydropregnenolone (1), allopregenolone (3), protocatechuic acid (4), vanillic acid (5), caffeic acid (6), and scopoletin (7). The structures of the isolated compounds were elucidated on the basis of their spectral data and chemical evidences. Compounds 1, 3, 4 were isolated for the first time from this plant. Cytotoxic activities of the isolated compounds were evaluated. Compound 1 exhibited significant cytotoxic activity against A375-S2, HeLa, SGC-7901, and Bel-7402 with $IC_{50}$ values of $13.1{\pm}0.9,\;21.5{\pm}1.0,\;40.2{\pm}0.7$, and $49.8{\pm}1.2\;{\mu}g/mL$, respectively.

• ETRI Journal
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• v.32 no.1
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• pp.102-111
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• 2010

Recently power attacks on RSA cryptosystems have been widely investigated, and various countermeasures have been proposed. One of the most efficient and secure countermeasures is the message blinding method, which includes the RSA derivative of the binary-with-random-initial-point algorithm on elliptical curve cryptosystems. It is known to be secure against first-order differential power analysis (DPA); however, it is susceptible to second-order DPA. Although second-order DPA gives some solutions for defeating message blinding methods, this kind of attack still has the practical difficulty of how to find the points of interest, that is, the exact moments when intermediate values are being manipulated. In this paper, we propose a practical second-order correlation power analysis (SOCPA). Our attack can easily find points of interest in a power trace and find the private key with a small number of power traces. We also propose an efficient countermeasure which is secure against the proposed SOCPA as well as existing power attacks.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.5 no.3
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• pp.204-211
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• 1993

Boundary element method is applied to simulate nonlinear water waves using Green's identity formula in a numerical wave flume. A system of linear equations is formulated from the governing equation and free surface boundary conditions in order to calculate velocity potential and water surface elevation at each nodal point. The velocity square terms are included in the dynamic free surface boundary condition. The free surface is treated as a moving boundary. the vertical variation of velocity potential being considered in calculating the time derivative of the velocity potential at the free surface. The present method is applied to simulate solitary wave and Stokes 2nd order wave, and shows excellent agreements with their theoretical values.

• International Journal of Ocean System Engineering
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• v.1 no.3
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• pp.120-135
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• 2011

This paper proposes a model based trajectory tracking control scheme for under-actuated underwater robotic vehicles. The difficulty in stabilizing a non-linear system using smooth static state feedback law means that the design of a feedback controller for an under-actuated system is somewhat challenging. A necessary condition for the asymptotic stability of an under-actuated vehicle about a single equilibrium is that its gravitational field has nonzero elements corresponding to non-actuated dynamics. To overcome this condition, we propose a continuous time-varying control law based on the direct estimation of vehicle dynamic variables such as inertia, damping and Coriolis & centripetal terms. This can work satisfactorily under commonly encountered uncertainties such as an ocean current and parameter variations. The proposed control law cancels the non-linearities in the vehicle dynamics by introducing non-linear elements in the input side. Knowledge of the bounds on uncertain terms is not required and it is conceptually simple and easy to implement. The controller parameter values are designed using the Taguchi robust design approach and the control law is verified analytically to be robust under uncertainties, including external disturbances and current. A comparison of the controller performance with that of a linear proportional-integral-derivative (PID) controller and sliding mode controller are also provided.

• Bulletin of the Korean Chemical Society
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• v.25 no.12
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• pp.1917-1923
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• 2004

Although the proteolytic activity of the Cu(II) complex of cyclen (Cyc) is greatly enhanced upon attachment to a cross-linked polystyrene (PS), the Cu(II)Cyc-containing PS derivatives reported previously hydrolyzed only a very limited number of proteins. The PS-based artificial metalloproteases can overcome thermal, mechanical, and chemical instabilities of natural proteases, but the narrow substrate selectivity of the artificial metalloproteases limits their industrial application. In the present study, artificial metalloproteases exhibiting broad substrate selectivity were synthesized by attaching Cu(II)Cyc to a PS derivative using linkers with various structures in an attempt to facilitate the interaction of various protein substrates with the PS surface. The new artificial metalloproteases hydrolyzed all of the four protein substrates (albumin, myoglobin, ${\gamma}$-globulin, and lysozyme) examined, manifesting $k_{cat}/K_m$ values of 28-1500 $h_{-1}M_{-1}$ at 50 $^{\circ}C$. The improvement in substrate selectivity is attributed to steric and/or polar interaction between the bound protein and the PS surface as well as the hydrophobicity of the microenvironment of the catalytic centers.

• Journal of computational fluids engineering
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• v.14 no.4
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• pp.67-77
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• 2009

This paper is a continuation of a recent development on the Hermite-based divergence-free element method and deals with a non-isothermal fluid flow driven by the buoyancy force in a square cavity with temperature difference across the two sides. Two Hermite functions are considered for numerical computations in this paper. One is a cubic function and the other is a quartic function. The degrees-of-freedom of the cubic Hermite function are stream function and its first and second derivatives for the velocity field, and temperature and its first derivatives for the temperature field. The degrees-of-freedom of the quartic Hermite function include two second derivatives and one cross derivative of the stream function in addition to the degrees-of-freedom of the cubic stream function. This paper presents a brief review on the Hermite based divergence-free basis functions and its finite element formulations for the buoyancy driven flow. The present algorithm does not employ any upwinding or a stabilization term. However, numerical values and contour graphs for major flow variables showed good agreements with those by De Vahl Davis[6].

• Archives of Pharmacal Research
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• v.26 no.5
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• pp.421-425
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• 2003

The aim of the present study was to evaluate the effects of two phospholipid permeation enhancers, lysophosphatidylcholine (LPC) and didecanoylphosphatidylcholine (DDPC), along with a fusidic acid derivative, sodium taurodihydrofusidate (STDHF) and ethanol (EtOH) on the buccal transport of propranolol hydrochloride (PPL) using an ex vivo buccal diffusion model. The permeation rate of [$^3 H$]PPL as measured by steady-state fluxes increased with increasing EtOH concentration. A significant flux enhancement (P＜0.05) was achieved by EtOH at 20 and 30 %v/v concentrations. At a 0.5 %w/v permeation enhancer concentration, the buccal permeation of [$^3 H$]PPL was significantly enhanced by all the enhancers studied (i.e., LPC, DDPC and STDHF) compared to the control (phosphate-buffered saline pH 7.4, PBS). LPC and DDPC displayed a greater degree of permeation enhancement compared with STDHF and EtOH-PBS mixtures with an enhancement ratio of 3.2 and 2.9 for LPC and DDPC, respectively compared with 2.0 and 1.5 for STDHF and EtOH:PBS 30:70 %v/v mixture, respectively. There was no significant difference between LPC and DDPC for the flux values and apparent permeability coefficients of [$^3$H]PPL. These results suggest that phospholipids are suitable as permeation enhancers for the buccal delivery of drugs.