• Journal of Food Hygiene and Safety
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• v.15 no.2
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• pp.137-143
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• 2000

The present study was performed to investigate the bioconcentration of phosphamidon and profenofos. The BCFs(bioconcentration factors), depuration rate constants and LC$_{50}$ for two pesticides in zebrafish(Brachydanio rerio) were measured by the flow-through system(OECD guideline 305). The results obtained are summarized as follows: The 24-hrs LC$_{50}$, 48-hrs LC$_{50}$, 72-hrs LC.n and 96-hrs LC$_{50}$ were more than 100 mg/l for phosphamidon. The concentration of phosphamidon in zebrafish reached an equilibrium in 12 hrs at low and high concentrations(0.2 mg/l and 1 mg/1). The average BCF values of phosphamidon were less than 1 at low(0.96, n=7) and high concentrations (0.89, n=7) after 12~168 hrs. Depuration rate constants of phosphamidon were 0.18 hr-1 and 0.21 hr-1, half-life of phosphamidon were 3.85 and 3.30 at low and high concentrations(0.2 mg/l and 1 mg/l), respectively, The concentrations of phosphamidon in zebrafish at low and high concentrations were rapidly decreased after 8(0.04 $\mu\textrm{g}$/g) and 12 hrs(0.07 $\mu\textrm{g}$/g). The 24-hrs LC$_{50}$, 48-hrs LC$_{50}$, 72-hrs LC$_{50}$ and 96-hrs LC$_{50}$ were 2.9, 2.6, 2.2 and 2.0 mg/1 for profenofos. The concentration of profenofos in zebrafish reached an equilibrium in 12 hrs at five-hundredth and one-hundredth concentration of 96-hrs LC$_{50}$(0.004 mgA and 0.02 mg/1). The average BCF values of profenofos were 141.9(n=7) and 111.3(n=7) at five-hundredth and one-hundredth concentration of 96-hrs LC$_{50}$(0.004 mg/l and 0.02 mg/1) after 12~168 hrs. Depuration rate constants of profenofos were 0.09 hr$^{-1}$ and 0.10 hr$^{-1}$, half-life of profenofos were 7.70 and 6.93 at five-hundredth and one-hundredth concentration of 96-hrs LC50(0.004 mg/l and 0.02 mg/1), respectively. The concentrations of profenofos in zebrafish at five-hundredth and one-hundredth concentration of 96-hrs LC$_{50}$ decreased agter 8(0.18 $\mu\textrm{g}$/g) and 12 hrs (0.19 $\mu\textrm{g}$/g). The LC$_{50}$ value in zebrafish showed that acute toxicity of profenofos was higher than that of phosphamidon. The BCF values of profenofos were 100 times higher than those of phosphamidon, and depuration rate of phosphamidon was two times faster than that of profenofos.

• Journal of Environmental Health Sciences
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• v.27 no.2
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• pp.37-42
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• 2001

Zebrafish(Brachdanio rerio)를 실험어류로 하여 methidathion 과 phosalone의 생물농축계수(bioconcentration factor : BCF)와 배설속도상수 (depuration rate constant) 및 LC$_{50}$를 측정하였다. Methidathion의 24, 48, 72, 96시간 LC$_{50}$는 각각 28.34, 35.98, 24.43, 22.03 mg/$\ell$로 측정되었다. Methidathion 0.22 mg/$\ell$(고농도)와 0.022 mg/$\ell$(저농도)에서 어류 체내에서의 농축정도는 두 농도군에서 각각 12시간 이후에 정류상태에 도달하여 72시간동안 거의 일정하였고, BCF값도 12시간에서 72시간 사이에 고농도와 저농도에서 8.72(n=4)와 11.25(n=4)로 조사되었다. 배설속도상수는 고농도와 저농도에서 6시간 이내에 모두 배설되어 배설속도상수를 구할 수 없었다. Phosalone의 24, 48, 72, 96시간 LC$_{50}$는 각각 3.76, 2.43, 1.86, 1.05 mg/$\ell$로 측정되었다. Zebrafish 체내에서의 농축정도와 BCF값은 고농도(0.01 mg/$\ell$)에서 12시간 이후에 정류상태에 도달하여 72시간동안 거의 일정하였고, BCF값은 12시간에서 72시간 사이에 48.88(n=4)로 측정되었다. 저농도(0.001 mg/$\ell$)에서는 실험 전기간동안 zebrafish 체내에서 phosalone이 검출되지 않아 BCF값을 산출할 수 없었다. Zebrafish 체내에서 phosalone(고농도)의 배설속도상수와 반감기를 구하기 위하여 6,12시간의 배설실험 결과 각각 0.17$hr^{-1}$과 4.01 시간이었다. Methidathion과 phosalone의 BCF값은 phosalone이 methidathion 보다 약 5배 정도 높게 나타났으며, 농약의 배설속도는 phosalone이 methidathion보다 빨랐다.