• Molecules and Cells
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• v.45 no.10
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• pp.718-728
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• 2022

Splicing factor B subunit 4 (SF3B4), a component of the U2-pre-mRNA spliceosomal complex, contributes to tumorigenesis in several types of tumors. However, the oncogenic potential of SF3B4 in lung cancer has not yet been determined. The in vivo expression profiles of SF3B4 in non-small cell lung cancer (NSCLC) from publicly available data revealed a significant increase in SF3B4 expression in tumor tissues compared to that in normal tissues. The impact of SF3B4 deletion on the growth of NSCLC cells was determined using a siRNA strategy in A549 lung adenocarcinoma cells. SF3B4 silencing resulted in marked retardation of the A549 cell proliferation, accompanied by the accumulation of cells at the G0/G1 phase and increased expression of p27, p21, and p53. Double knockdown of SF3B4 and p53 resulted in the restoration of p21 expression and partial recovery of cell proliferation, indicating that the p53/p21 axis is involved, at least in part, in the SF3B4-mediated regulation of A549 cell proliferation. We also provided ubiquitination factor E4B (UBE4B) is essential for p53 accumulation after SF3B4 depletion based on followings. First, co-immunoprecipitation showed that SF3B4 interacts with UBE4B. Furthermore, UBE4B levels were decreased by SF3B4 depletion. UBE4B depletion, in turn, reproduced the outcome of SF3B4 depletion, including reduction of polyubiquitinated p53 levels, subsequent induction of p53/p21 and p27, and proliferation retardation. Collectively, our findings indicate the important role of SF3B4 in the regulation of A549 cell proliferation through the UBE4B/p53/p21 axis and p27, implicating the therapeutic strategies for NSCLC targeting SF3B4 and UBE4B.

• Journal of the Korean institute of surface engineering
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• v.31 no.6
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• pp.407-416
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• 1998

• Proceedings of the Microbiological Society of Korea Conference
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• 2005.05a
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• pp.56-57
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• 2005

• Proceedings of the Korean Nuclear Society Conference
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• 2003.05a
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• pp.55.1-55.1
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• 2003

• The Journal of Korea Robotics Society
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• v.4 no.2
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• pp.97-104
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• 2009

Recently, simultaneous localization and mapping (SLAM) approaches employing Rao-Blackwellized particle filter (RBPF) have shown good results. However, due to the usage of the accurate sensors, distinct particles which compensate one another are attenuated as the RBPF-SLAM continues. To avoid this particle depletion, we propose the strategic games to assign the particle's payoff which replaces the importance weight in the current RBPF-SLAM framework. From simulation works, we show that RBPF-SLAM with the strategic games is inconsistent in the pessimistic way, which is different from the existing optimistic RBPF-SLAM. In addition, first, the estimation errors with applying the strategic games are much less than those of the standard RBPF-SLAM, and second, the particle depletion is alleviated.

• Korean Journal of Soil Science and Fertilizer
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• v.47 no.2
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• pp.80-84
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• 2014

Phosphorus (P) is an essential nutrient for all living organisms. P is mostly obtained from mined rock phosphate. However, existing rock phosphate reserves could be exhausted in the next 50-100 years. As Korea is totally dependent on imported rock phosphate, we should seek for solution to overcome the P depletion by efficient use and recycling. For this, this study suggested a P flow model to identify the location and flow route of P in urban area based on traditional material flow analysis. The type of P entering the urban areas are fertilizer, food and feed. Each type of P is used in agriculture, human consumption and animal husbandry. After going through each process, P is moved to waste management facilities within food waste, excreta and sewage. Some portion of P in waste are buried, incinerated and discharged, which can be reservoir of P in the future.

• Journal of the Institute of Electronics and Information Engineers
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• v.51 no.1
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• pp.225-232
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• 2014

Optical parametric amplification has been analyzed for the Cerenkov-idler configuration in planar waveguides. The coupled-mode theory is employed for the analysis. The coupled-mode equations are derived and the approximate analytic solution is obtained for no pump depletion. From the analytic solution, it is shown that the signal power gain can be enhanced as the Cerenkov radiation angle of the idler approaches to zero. The numerical example is also shown for the effect of the Cerenkov radiation angle approaching zero.

• Journal of Korean Institute of Industrial Engineers
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• v.11 no.1
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• pp.33-39
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• 1985

A stochastic model for an inventory system in which depletion of stock takes place due to random demand as well as random loss of items is studied under the assumption that the intervals between successive unit demands, as well as those between successive unit losses are independently and identically distributed random variables having negative exponential distribution with respective parameters. We have derived the steady state probability distribution of the stock level assuming instantaneous delivery of order under (s, S) inventory policy. Also we have derived total expected cost expression and the necessary conditions to be satisfied for an optimal solution.

• Journal of Microbiology and Biotechnology
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• v.1 no.1
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• pp.79-83
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• 1991

The effects of phosphate concentration in the medium, feeding of biosynthetic precursor, and the addition of exogenous berberine on cell growth and berberine production were studied in cell suspension cultures of Thalictrum rugosum. The depletion of phosphate in the medium enhanced the specific productivity up to twofold with significant release of berberine into the medium. Extracellular berberine was 19% of the total in the culture without phosphate while it was 2-5% of total berberine in the culture with even low amounts of phosphate. Precursor feeding was not effective in enhancing alkaloid formation. Initial presence of exogenous berberine did not have much effect on cell growth and alkaloid production. It was found that the cells have the capacity to take up large quantities of berberine. When $500{\;}mg{\cdot}l^{-1}$ of berberine was added exogenously at the beginning, 81% of total berberine was found in the cells.

• Proceedings of the KWS Conference
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• 2002.10a
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• pp.65-69
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• 2002

An Al film deposited on the Kovar alloy substrate was anodically-bonded to the borosilicate glass, and the bond interfaces was closely investigated by transmission electron microscopy. Al oxide was found to form a layer ~l0 nm thick at the bond interface, and fibrous structure of the same oxide was found to grow epitaxially in the glass from the oxide layer. The fibrous structure grew with the bonding time. The mechanism of the formation of this fibrous structure is proposed on the basis of the migration of Al ions under the electric field. Penetration of Al into glass beyond the interfacial Al oxide was not detected. The comparison of the amount of excess oxygen ions generated in the alkali depletion layer with that incorporated in the Al oxide suggests that the growth of the alkali-ion depletion layer is controlled by the consumption of excess oxygen to form the interfacial Al oxide.