• Structural Engineering and Mechanics
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• 제38권2호
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• pp.171-193
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• 2011

One of the possible alternatives of simulation-based time-dependent reliability assessment of pre-stressed biconcave and biconvex cable trusses, the Monte Carlo method, is applied in this paper. The influence of an excessive deflection of cable truss (caused by creep of cables and rheologic changes) on its time-dependent serviceability is investigated. Attention is given to the definition of the basic random variables and their statistical functions (basic, mutually dependent random variables such as the pre-stressing forces of the bottom and top cable, structural geometry, the Young's modulus of elasticity of the cables, and the independent variables, such as permanent load, wind, snow and thermal actions). Then, the determination of the response of the cable truss to the loading effects, and the definition of the limiting values considering serviceability of the structure are performed. The potential of the method, using direct Monte Carlo technique for simulation-based time-dependent reliability assessment as a powerful tool, is emphasized. Results obtained by the First order reliability method (FORM) are compared with those obtained by the Monte Carlo simulation technique.

• 생약학회지
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• 제15권1호
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• pp.24-29
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• 1984

It was previously reported from our laboratory that the rate of deterioration of contractile force was slower in the heart of the ginseng extract treated rats. It was also found that ginseng may have an ability to sustain the normal function of the heart by sustaining Ca accumulation by sarcoplasmic reticulum. $Ca^{++}-dependent$ ATPase plays the central role in movement of $Ca^{++}$ ion from sarcoplasm into sarcoplasmic reticulum. In this investigation, the fragment of sarcoplasmic reticulum was prepared from rat heart treated with ginseng water extract orally 100mg/kg/day for 7 to 10 days and from normal rat heart. $Ca^{++}-dependent$ APTase activity was estimated by a modified method of Fiske and Subbarow's procedure. Experimental groups were divided into 6 groups, depending on the preincubation time, 5, 30 and 60min. at ${25}^{\circ}C$ and ${37}^{\circ}C$ respectively. In both of the groups of ${25}^{\circ}C$ and ${37}^{\circ}C$, $Ca^{++}-dependent$ ATPase activities of the ginseng treated rat hearts were higher than that of normal hearts. Therefore, it can be concluded that $Ca^{++}-dependent$ ATPase activities in sarcoplasmic reticulum of rat hearts were increased by the treatment with ginseng extract.

• Computers and Concrete
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• 제23권2호
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• pp.133-143
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• 2019

The paper presents the experimental investigations into the effect of ground granulated blast furnace slag (GGBFS) on the time-dependent tensile strength of concrete. The splitting and flexural tensile strength of concrete was determined at the ages of 3, 7, 28, 56, 90, 150 and 180 days using the cylindrical and prism specimens respectively for plain and GGBFS concrete. The amount of cement replacement by GGBFS was 0%, 40% and 60% on the weight basis. The maximum curing age was kept as 28 days. The results showed that the splitting and flexural tensile strength of concrete containing GGBFS has been found lower than the plain concrete at all ages and for all mixes. The tensile strength of 40 percent replacement has been found higher than the 60 percent at all ages and for all mixes. The rate of gain of splitting and flexural tensile strength of 40 percent GGBFS concrete is found higher than the plain concrete and 60 percent GGBFS concrete at the ages varying from 28 to 180 days. The experimental results of time-dependent tensile strength of concrete are compared with the available models. New models for the prediction of time-dependent splitting and flexural tensile strength of concrete containing GGBFS are proposed. The present experimental and analytical study will be helpful for the designers to know the time-dependent tensile properties of GGBFS concrete to meet the design requirements of liquid retaining reinforced and pre-stressed concrete structures.

• Laboraroty Animal Research
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• 제34권4호
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• pp.264-269
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• 2018

Cell cycle dysfunction can cause severe diseases, including neurodegenerative disease and cancer. Mutations in cyclin-dependent kinase inhibitors controlling the G1 phase of the cell cycle are prevalent in various cancers. Mice lacking the tumor suppressors $p16^{Ink4a}$ (Cdkn2a, cyclin-dependent kinase inhibitor 2a), $p19^{Arf}$ (an alternative reading frame product of Cdkn2a,), and $p27^{Kip1}$ (Cdkn1b, cyclin-dependent kinase inhibitor 1b) result in malignant progression of epithelial cancers, sarcomas, and melanomas, respectively. Here, we generated knockout mouse models for each of these three cyclin-dependent kinase inhibitors using engineered nucleases. The $p16^{Ink4a}$ and $p19^{Arf}$ knockout mice were generated via transcription activator-like effector nucleases (TALENs), and $p27^{Kip1}$ knockout mice via clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9 (CRISPR/Cas9). These gene editing technologies were targeted to the first exon of each gene, to induce frameshifts producing premature termination codons. Unlike preexisting embryonic stem cell-based knockout mice, our mouse models are free from selectable markers or other external gene insertions, permitting more precise study of cell cycle-related diseases without confounding influences of foreign DNA.

• Advances in materials Research
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• 제12권1호
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• pp.15-29
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• 2023

In this paper, fracture analysis of a continuously inhomogeneous arch structure with two longitudinal cracks is developed in terms of the time-dependent strain energy release rate. The arch under consideration exhibits non-linear creep behavior. The cross-section of the arch is a rectangle. The material is continuously inhomogeneous along the thickness of the cross-section. The arch is loaded by two bending moments applied at its end sections. The mechanical behavior of the material is described by using a non-linear stress-strain-time relationship. The two longitudinal cracks are located symmetrically with respect to the mid-span of the arch. Due to the symmetry, only half of the arch is considered. Time-dependent solutions to strain energy release rate are obtained by analyzing the balance of the energy. For verification, time-dependent solutions to the strain energy release rate are derived also by considering the time-dependent complementary strain energy. The evolution of the strain energy release rate with the time is analyzed. The effects of material inhomogeneity, locations of the two cracks along the thickness of the arch and the magnitude of the external loading on the time-dependent strain energy release rate are evaluated.

• Geomechanics and Engineering
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• 제28권2호
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• pp.171-180
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• 2022

Time-dependent stress-strain behaviour significantly influences the compressibility characteristics of the clayey soil. In this paper, a series of oedometer tests were conducted in two loading patterns and investigated the time-dependent compressibility characteristics of Indian Montmorillonite Clay, also known as black cotton soil (BC) soil, during loading-unloading stages. The experimental data are analyzed using a new non-linear function of the Elasto-Visco-Plastic Model considering Swelling behaviour (EVPS model). From the experimental result, it is found that BC soil exhibits significant time-dependent behaviour during creep compared to the swelling stage. Pore water entrance restriction due to consolidated overburden pressure and decrease in cation hydrations are responsible factors. Apart from it, particle sliding is also evident during creep. The time-dependent parameters like strain limit, creep coefficient and C_αe/C_c are observed to be significant during the loading stage than the swelling stage. The relationship between creep coefficients and applied stresses is found to be nonlinear. The creep coefficient increases significantly up to 630 kPa-760 kPa (during reloading), and beyond it, the creep coefficient decreases continuously. Several parameters like loading duration, the magnitude of applied stress, loading history, and loading path have also influenced secondary compressibility characteristics. The time-dependent compressibility characteristics of BC soil are presented and discussed in detail.

• Geomechanics and Engineering
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• 제34권2호
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• pp.197-208
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• 2023

Rate-dependent mechanical response of sand, subjected to loading of medium to high strain rate range, is of interest for several civilian and military applications. Such rate-dependent response can vary significantly based on the initial density state of the sand, applied confining pressure, considered strain rate range, drainage condition and sand morphology. A numerical study has been carried out employing a recently proposed visco-plastic constitutive model to explore the rate-dependent mechanical behaviour of Toyoura sand under drained triaxial loading condition. The model parameters have been calibrated using the experimental data on Toyoura sand available in published literature. Under strain rates higher than a reference strain rate, the simulation results are found to be in good agreement with the experimentally observed characteristic shearing behaviour of sand, which includes increased shear strength, pronounced post-peak softening and suppressed compression. The rate-dependent response, subjected to intermediate strain rate range, has further been assessed in terms of enhancement of peak shear strength and peak friction angle over varying initial density and confining pressure. The simulation results indicate that the rate-induced strength increase is highest for the dense state and such strength enhancements remain nearly independent of the applied confinement level.

• The Korean Journal of Physiology
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• 제27권2호
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• pp.115-122
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• 1993

There is evidence that noradrenaline enhances spontaneous contractions dose-dependently in guinea-pig antral circular muscle. To investigate the mechanism of this excitatory action, slow waves and membrane currents were recorded using conventional microelectrode techniques in muscle strips and the whole cell patch clamp technique in isolated gastric myocytes. On recording slow waves, noradrenaline $(10^{-5}\;M)$ induced the hyperpolarization of the membrane potential, although the shape of the slow waves became tall and steep. Also, spike potentiaIs occurred at the peaks of slow waves. These changes were completely reversed by administration of phentolamine $(10^{-5}\;M),\;an\;{\alpha}-adrenoceptor$ blocker. Noradrenaline-induced hyperpolarization was blocked by apamin $(10^{-7}\;M)$, a blocker of a class of $Ca^{2+}\;-dependent\;K^+$ channels. To investigate the mechanisms for these effects, we performed whole cell patch clamp experiments. Norndrenaline increased voltage-dependent $Ca^{2+}$ currents in the whole range of test potentials. Noradrenaline also increased $Ca^{2+}\;-dependent\;K^+$\;currents, and this effects was abolished by apamin. These results suggest that the increase in amplitude and the generation of spike potentials on slow waves was caused by the activation of voltage-dependent $Ca^{2+}$ channel via adrenoceptors, and hyperpolarization of the membrane potential was mediated by activation of apamin-sensitive $Ca^{2+}\;-dependent\;K^+\;channels$.

• The Korean Journal of Physiology
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• 제27권2호
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• pp.139-150
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• 1993

We have reported that dopamine potentiates spontaneous contractions dose-dependently in guinea-pig antral circular muscle strips (Hwang et al, 1991). To clarify the underlying excitatory mechanism of dopamine on the gastric smooth muscle, the effects of dopamine on voltage-dependent $Ca^{2+}\;currents\;and\;Ca^{2+}\;-dependent\;K^+\;currents$ were observed in enzymatically dispersed guinea-pig gastric myocytes using the whole-cell voltage-clamp technique. Experiments were also done using isometric tension recording and conventional intracellular microelectrode techniques. 1) The effect of dopamine on the spontaneous contraction of antral circular muscle strips of the guinea-pig was excitatory in a dose-dependent manner, and was blocked by phentolamine, an ${\alpha}-adrenoceptor$ blocker. 2) The slow waves were not changed by dopamine. 3) The voltage-operated inward $Ca^{2+}$ current was not influenced by dopamine. 4) The $Ca^{2+}\;-dependent\;K^+$ outward current, which might reflect the changes of intracellular calcium concentration, was enhanced by dopamine. This effect was abolished by phentolamine. 5) The enhancing effect of dopamine on the $Ca^{2+}\;-dependent\;K^+$ current disappeared with heparin which is known to block the action of $InsP_3$. From these results, it is suggested that dopamine acts via $InsP_3-mediated\;Ca^{2+}$ mobilization from intracellular stores and such action potentiates the spontaneous contraction of guinea-pig gastric smooth muscle.

• 대한기계학회논문집
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• 제18권3호
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• pp.548-554
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• 1994

The high temperature fatigue crack growth behavior of SUS 304 stainless steel at $550^{\circ}C$ and $650^{\circ}C$ was investigated under various kinds of stress ratio and frequency in sinusoidal waveform on the basis of the non-linear fracture mechanics. The result arranging crack growth rate by modified J-integral J' showed influence of stress ratio and frequency. All the data obtained under the test at $550^{\circ}C$ were plotted within data band of da/dN-${\triangle}J_f$ relationship for cycle-dependent crack growth. On the basis of static creep and cycle-dependent data band; both time- and cycle-dependent crack growth behavior was observed under loading conditions at $650^{\circ}C$, but cycle-dependent crack growth behavior predominantly appeared and time-dependent crack growth behaviour was little observed under loading conditions at $550^{\circ}C$. Fractographic examinations for fracture surface indicated that the fracture mode was generally transgranular. The stripes were found on fracture surface and each stripe was accompanied by a crack tip blunting and an abrupt increase in the load-point displacement. The $J'_{an}$ had a validity in case of $650^{\circ}C, but scarcely had it in case of $550^{\circ}C$.