• Title/Summary/Keyword: Denitrifcation

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Evaluation of Denitrification Efficiency and Functional Gene Change According to Carbon(Fumarate) Concentration and Addition of Nitrate Contaminated-soil in Batch System (회분식반응조 실험을 통한 탄소원(Fumarate) 주입조건에 따른 지하수 중 탈질율 및 탈질 관련 기능성 유전자 분석)

  • Park, Sunhwa;Kim, Hyun-Koo;Kim, Moon-su;Lee, Gyeong-Mi;Jeon, Sang-Ho;Song, Dahee;Kim, Deok-hyun;Kim, Young;Kim, Tae-seung
    • Journal of Soil and Groundwater Environment
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    • v.20 no.7
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    • pp.80-89
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    • 2015
  • Nitrate is on the most seriou pollutant encountered in shallow groundwater aquifer in agricultural area. There are various remediation technologies such as ion exchange, reverse osmosis, and biological denitrification to recover from nitrate contamination. Biological denitrification by indigenous microorganism of the technologies has been reviewed and applied on nitrate contaminated groundwater. In this work, we selected the site where the annual nitrate (NO3) concentration is over 105 mg/L and evaluated denitrification process with sampled soil and groundwater from 3 monitoring wells (MW4, 5, 6). In the results, the nitrate degradation rate in each well (MW 4, 5, and 6) was 25 NO3 mg/L/day, 6 NO3 mg/L/day, and 3.4 NO3 mg/L/day, respectively. Nitrate degradation rate was higher in batch system treated with 2 times higher fumarate as carbon source than control batch system (0.42M fumrate/1M NO3), comparing with batch system with soil sample. This result indicates that increase of carbon source is more efficient to enhance denitrification rate than addition of soil sample to increase microbial dynamics. In this work, we also confirmed that monitoring method of functional genes (nirK and nosZ) involved in denitrification process can be applied to evaluated denitrifcation process possibility before application of field process such as in-situ denitrification by push-pull test.

Effect of Feeding Pattern and Anaerobic Fill Time on the Denitrifcation and Sludge Settling Ability in the SBR Process (SBR 공정에서 유입수 주입방식과 비 포기 유입수 주입시간이 탈질효율과 슬러지 침강성에 미치는 영향)

  • Lee, Sang-Min;Nam, Se-Yong
    • Journal of Korean Society of Environmental Engineers
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    • v.27 no.7
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    • pp.719-725
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    • 2005
  • Anaerobic fill time and feeding pattern in SBR operation were investigated to find way of minimizing poor nitrogen removal efficiency in BNR process without external carbon addition. The three types of the modified SBR operations that were CO-SBR, IA-SBR, and SF-SBR were tested by lab-scale and pilot-scale SBR processes($2\;m^3/day$). In addition, practical equation for biological nitrogen removal was suggested and the equation considered the effect of ratio of fill volume over whole SBR volume and the ratio of step-feed in SBR. The denitrification efficiency of the SF-SBR was best among the three SBRs and followed by IA-SBR, and CO-SBR. The efficiency was 95%, 61%, and 19%, respectively. Looking at the change of sludge floc density by the length of anaerobic fill time, the density of sludge floc at 1 hour and 2 hours of anaerobic fill time were greater than 3 hours of one. The floc size distributions were $100{\sim}300\;{\mu}m$ and $200{\sim}400\;{\mu}m$ with respect to anaerobic fill time 2 hours and 3 hours, respectively.

Effect of Unexpected Foaming Incident on Nitrogen Removal in a Vertical Subsurface Wetland (수직지하 흐름형 습지에서 거품발생이 질소제거에 미치는 영향)

  • Cheng, Jing;Guerra, Heidi B.;Kim, Youngchul
    • Journal of Wetlands Research
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    • v.21 no.4
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    • pp.334-343
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    • 2019
  • A lab-scale vertical flow subsurface (VFS) wetland composed of three parallel columns with polypropylene synthetic fiber as main substrate was operated. Piggery stormwater diluted from swine excreta was fed to the wetland on the basis of three different hydraulic regimes or hydraulic retention time (HRT) of 2, 4, and 8 days with daily internal recirculation. Then, monitoring of common water quality parameters was carried out. Unexpectedly, an increase of effluent COD concentration accompanying the appearance of foams was observed during a distinct period in the wetland with HRT 2, 4, and 8 days, successively. Subsequently, a series of experiments was conducted to investigate the origin of the foams. Foams and the increase of COD concentration were found to be induced by the release of organic matter from the synthetic polypropylene fiber which was fed with piggery stormwater. Meanwhile, nitrogen removal was found to be enhanced during a period which overlapped the distinct foaming period signifying that foaming played two important functions in biological nitrogen removal. Foams which form rapidly and then burst easily could hold up and then release oxygen for nitrification. Foams which contain organic surfactants could serve as carbon sources for denitrification as well. Hence, nitrogen removal was enhanced during the foaming stage. After that, COD concentration decreased slowly to a level prior to the foaming stage, and nitrogen removal efficiency declined as well.