• Research in Plant Disease
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• v.10 no.1
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• pp.25-29
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• 2004

Over 98％ of Dendranthema x grandiflorum plants growing under a glasshouse in Gumi, Korea were infested with Meloidogyne hapla. Dendranthema x grandiflorum cv, 'Ardilo' had fewer eggmass (3.3/g root), followed by cv. 'Euro' (6.5), and cv. 'Monarisa' had the most eggmasses(17). The coefficient correlation between plant growth and nematode reproduction(number of eggmass per plant) indicated that nematode reduced chlorophyll content in leaf (r = -0.418, p = 0.059; r = -0.463, p = 0.040). Number of flowers, number of leaves and stem height to flower were positively correlated with flower weight(p < 0.05).

• Journal of Plant Biotechnology
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• v.30 no.4
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• pp.335-339
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• 2003

Several experiments were carried out to transfer LEAFY gene to Dendranthema grandiflora 'Shuho-no-chikara' by Agrobacterium LBA4404 carrying pSK109 encoding LEAFY gene. Kanamycin 10mg/L was used in first selection medium, and 20mg/L in the second one. Co-culture for 3 days was more effective in increasing transformation efficiency than that for 7 days. The transformation efficiency by Agrobacterium LBA4404 carrying pSK109 encoding LEAFY gene was about 2.8％ until the second selection, but only 0.13％ of shoots (two plants) was confirmed as a transgenic plants in Southern analysis. The escape of putative transformants was occured seriously in the process of selections, PCR analysis for confirming of neomycin phosphotransferaseII (npt II), and Southern analysis for LEAFY gene. One transgenic plant appeared 7 days'early flowering in field.

• Journal of Plant Biotechnology
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• v.6 no.1
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• pp.25-37
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• 2004

Successful genetic transformation of plants requires non-chimeric selection of transformed tissues and their subsequent regeneration. With rare exceptions, most transformation protocols still rely heavily on antibiotics for selecting transgenic cells that contain an antibiotic-degrading selectable marker gene. Here, the morphogenic capacity of in-vitro explants of chrysanthemnum and tobacco stems and leaves (control and transgenic) changed with the addition of aminoglycoside antibiotics (AAs), In a test of 6 AAs, phytotoxicity occurred at concentrations of 10 to 25 and 50 to 100$\mu\textrm{g}$ $mL^{-1}$ in chrysanthemum and tobacco explants, respectively. Light conditions as well as explant source and size also had significant effects. The use of transverse thin cell layers (tTCLs), in conjunction with high initial AA selection levels, supported the greatest regeneration of transgenic material (adventitious shoots or callus) and the lowest number of escapes. Flow-cytometric analyses revealed no endodu-plication in chrysanthemum, even at high AA levels. However, this phenomenon was observed in tobacco calli(8C or more), even at low AA concentrations (i.e., 5 to 10 $\mu\textrm{g}$ mL$^{-1}$ ).