• Mycobiology
• /
• 제38권1호
• /
• pp.26-32
• /
• 2010

The cyclic AMP (cAMP) pathway plays a major role in growth, sexual differentiation, and virulence factor synthesis of pathogenic fungi. In Cryptococcus neoformans, perturbation of the cAMP pathway, such as a deletion in the gene encoding adenylyl cyclase (CAC1), causes defects in the production of virulence factors, including capsule and melanin production, as well as mating. Previously, we performed a comparative transcriptome analysis of the Ras- and cAMP- pathway mutants, which revealed 163 potential cAMP-regulated genes (38 genes at a 2-fold cutoff). The present study characterized the role of one of the cAMP pathway-dependent genes (serotype A identification number CNAG_ 06576.2). The expression patterns were confirmed by Northern blot analysis and the gene was designated cAMP-regulated gene 1 (CAR1). Interestingly, deletion of CAR1 did not affect biosynthesis of any virulence factors and the mating process, unlike the cAMP-signaling deficient cac1$\Delta$ mutant. Furthermore, the car1$\Delta$ mutant exhibited wild-type levels of the stress-response phenotype against diverse environmental cues, indicating that Car1, albeit regulated by the cAMP-pathway, is not essential to confer a cAMP-dependent phenotype in C. neoformans.

• 대한음성학회:학술대회논문집
• /
• 대한음성학회 2002년도 11월 학술대회지
• /
• pp.81-85
• /
• 2002

We present a statistical analysis of Korean phonological variations using automatic generation of phonetic transcription. We have constructed the automatic generation system of Korean pronunciation variants by applying rules modeling obligatory and optional phonemic changes and allophonic changes. These rules are derived from knowledge-based morphophonological analysis and government standard pronunciation rules. This system is optimized for continuous speech recognition by generating phonetic transcriptions for training and constructing a pronunciation dictionary for recognition. In this paper, we describe Korean phonological variations by analyzing the statistics of phonemic change rule applications for the 60,000 sentences in the Samsung PBS(Phonetic Balanced Sentence) Speech DB. Our results show that the most frequently happening obligatory phonemic variations are in the order of liaison, tensification, aspirationalization, and nasalization of obstruent, and that the most frequently happening optional phonemic variations are in the order of initial consonant h-deletion, insertion of final consonant with the same place of articulation as the next consonants, and deletion of final consonant with the same place of articulation as the next consonants. These statistics can be used for improving the performance of speech recognition systems.

• 음성과학
• /
• 제9권1호
• /
• pp.193-206
• /
• 2002

The aim of this study is to find out how the labiovelar onglide /w/ in English kwV sequences that have minimal pairs with kV sequences is pronounced differently among Korean EFL learners based on acoustic evidence. This study tries to identify /w/ sound in English kwV sequences through spectrograms and to examine the duration ratios of each segment in kwV words to compare the patterns of an English native speaker with those of Korean speakers of English. In spectrographic analyses, the complete deletion of /w/ and partial pronunciation of /w/ dubbed [$k^{w}$] were identified as well as the targetappropriate production of /w/. The general production patterns with respect to the duration ratios in English [kw] sequence words showed that the subjects who produced /w/ had similar ratio patterns that the native speaker had in that the vowel duration ratio in kwV sequences was shorter than that in kV sequences. By contrast, the subjects who deleted [w] had a long ratio of the onset [$k^{h}$] while the speaker with a partial pronunciation of /w/ had a long ratio of the following vowel.

• Applied Microscopy
• /
• 제24권1호
• /
• pp.77-85
• /
• 1994

In order to investigating the pulmonary toxicity of the O-chlorobenzyledene malononitrile (CS), lacrimating agent, $2.6g/m^3$ of CS was inhalated to Sprague-Dawley rats in the plastic chamber for 20 minutes. The ultrastructural changes of type II pneumocytes in the lung were observed with Hitachi 600 transmission electron microscope. The results obtained were as follows: 1. 3 hours after exposure to CS the fusion of surface microvilli, dilatation of cristernae of the rough endoplasmic reticulum, atrophy of Golgi complex and condensation, deletion of lamellated membranes in lamellar bodies were observed in type II pneumocytes. 2. One and 2 days after CS-exposure, disorganization of mitochondrial double membranes, fragmentations of rough endoplasmic reticulum were found in the great alveolar cells. In addition, decrease in amount of polyribosome granules and deletion or condensation of lamellated membranes in lamellar bodies were also observed. 3. 4 days after exposure to CS, the type II pneumocyte revealed new whorled lamellar membranes in lamellar bodies, a few intact rough endoplasmic reticulum and restoration of polyribosome granules. It is consequently suggested that CS induces degenerative changes of cytoplasmic organelles in the type II pneumocytes.

• Molecules and Cells
• /
• 제19권2호
• /
• pp.256-261
• /
• 2005

In silico analysis of genome of the cyanobacterium Synechocystis sp. PCC 6803 identified two genes, slr0329 and sll0593, that might participate in glucose (Glc) phosphorylation (www.kazusa.or.jp/cyano). In order to determine the functions of these two genes, we generated deletion mutants, and analyzed their phenotypes and enzymatic activities. In the presence of 10 mM Glc, wild-type (WT) and slr0329 defective strain (M1) grew fast with increased respiratory activity and NADPH production, whereas the sll0593 deletion mutant (M2) failed to show any of the Glc responses. WT and M1 were not significantly different in their glucokinase activity, but M2 had 90% less activity. Therefore, we propose that Sll0593 plays a major role in the phosphorylation of glucose in Synechocystis cells.

• Molecules and Cells
• /
• 제27권1호
• /
• pp.89-97
• /
• 2009

We investigate the molecular and cellular etiologies that underlie the deletion of the six amino acid residues (${\Delta}F323-Y328$; ${\Delta}FY$) in human torsin A (HtorA). The most common and severe mutation involved with early-onset torsion dystonia is a glutamic acid deletion (${\Delta}E$ 302/303; ${\Delta}E$) in HtorA which induces protein aggregates in neurons and cells. Even though ${\Delta}FY$ HtorA forms no protein clusters, flies expressing ${\Delta}FY$ HtorA in neurons or muscles manifested a similar but delayed onset of adult locomotor disability compared with flies expressing ${\Delta}E$ in HtorA. In addition, flies expressing ${\Delta}FY$ HtorA had fewer aberrant ultrastructures at synapses compared with flies expressing ${\Delta}E$ HtorA. Taken together, the ${\Delta}FY$ mutation in HtorA may be responsible for behavioral and anatomical aberrations in Drosophila.

• 방송공학회논문지
• /
• 제22권6호
• /
• pp.734-743
• /
• 2017

본 논문에서는 동영상의 압축 정보를 이용하여 동영상 조작 시 발생하는 특징 패턴을 분석하여 동영상의 삭제 여부를 검출하는 기법에 대해 소개한다. 제안 방식에서는 최근 표준 코덱으로 개발되어 향후 널리 사용될 것으로 예상되는 HEVC 코덱을 이용한다. 우선 조작된 동영상과 그렇지 않은 동영상의 HEVC 부호화 패턴 중 분류하기가 용이한 여러 패턴들을 분석하여 특징벡터로 선정하고, 선정된 특징벡터를 기계학습을 통해 학습하여 두 그룹 간의 분류 기준을 모델링하여 동영상에 대한 삭제 여부를 판단한다. 실험 결과, 제안한 방식이 이전의 연구 결과에 비해 HEVC 코덱 환경에서 더욱 효과적으로 삭제 여부를 판단함을 확인하였다.

• BMB Reports
• /
• 제43권10호
• /
• pp.693-697
• /
• 2010

Hereditary non-polyposis colorectal cancer (HNPCC) is an autosomal dominant syndrome characterized by predisposition to early-onset cancers. HNPCC is caused by heterozygous loss-of-function mutations within the mismatch repair genes MLH1, MSH2, MSH6, PMS1, and PMS2. We genotyped the MLH1 and MSH2 genes in patients suffering from Lynch syndrome and in 11 unrelated patients who were diagnosed with colorectal cancer and had subsequently undergone surgery. Five Lynch syndrome patients carried germline mutations in MLH1 or MSH2. Two of these were identified as known mutations in MLH1: deletion of exon 10 and a point mutation (V384D). The remaining three patients exhibited novel mutations: a duplication (937_942dupGAAGTT) in MLH1; deletion of exons 8, 9, and 10; and a point mutation in MLH1 (F396I) combined with multiple missense mutations in MSH2 (D295G, K808E, Q855P, and I884T). The findings underline the importance of efficient pre-screening of conspicuous cases.

• Journal of Microbiology and Biotechnology
• /
• 제20권4호
• /
• pp.678-682
• /
• 2010

The deletion of sti from the Streptomyces plasmid pIJ101 made its derivative pHZ1358 an efficient vector for gene disruption and replacement. Here, pHZ1358 was further optimized by the construction of a derivative plasmid pJTU1278, in which a cassette carrying multiple cloning sites and a lacZ selection marker were introduced for convenient plasmid construction in E. coli. In addition, the oriT region of pJTU1278 was also deleted, generating a vector (pJTU1289) that can be used specifically for PCR-targeting. The efficient usage of these vectors was demonstrated by the deletion of the gene involved in avermectin biosynthesis in S. avermitilis.

• 인지과학
• /
• 제11권1호
• /
• pp.9-24
• /
• 2000

본고는 한국어 대화체에서 자주 관찰되는 논항 명사구의 생략현상에 대한 분석을 제시한다. 약 한 시간 분량의 라디오 대담 프로그램을 녹취, 전사하여 획득한 자료를 중심으로, 한국어 대화체에서 논항 명사구의 생략 빈도 및 그 생략 논항의 선행사를 지시대상의 유형을 분류한 통계를 제시한다. 나아가 센터링 이론을 적용하여 이들 생략 논항의 선행사를 결정하는 노력에 있어 청/화자와 일반인을 지칭하는 영형 대명사를 센터링 이론의 적용대상에서 제외시킬 것을 제안하고, 이렇게 제외된 청/화자 지칭 영형 대명사가 보이는 언어적 단서를 추적하여 제시한다. 또한 센터의 순위 및 전이 유형을 결정하는 과정에서 반드시 고려해야할 한국어의 특성에 대해 주제와 주어의 차이. 구문분석의 영향, 그리고 세상지식과의 충돌 등을 중심으로 지적한다.