• The Korean Journal of Physiology and Pharmacology
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• 제5권5호
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• pp.413-421
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• 2001

An impaired smooth muscle cell (SMC) relaxation of coronary artery by alteration of $K^+$ channels would be the most potential explanation for reduced coronary reserve in left ventricular hypertrophy (LVH), however, this possibility has not been investigated. We performed morphometrical analysis of the coronary artery under electron microscopy and measured $Ca^{2+}-activated\;K\;(K_{Ca})$ currents and delayed rectifier K $(K_{dr})$ currents by whole-cell and inside-out patch-clamp technique in single coronary arterial SMCs from rabbits subjected to isoprenaline-induced cardiac hypertrophy. Coronary arterial SMCs underwent significant changes in ultrastructure. The unitary current amplitude and the open-state probability of $K_{Ca}$ channel were significantly reduced in hypertrophy without open-time and closed-time kinetic. The concentration-response curve of $K_{Ca}$ channel to $Ca^{2+}$ is shifted to the right in hypertrophy. The reduction in the mean single channel current and increase in the open channel noise of $K_{Ca}$ channel by TEA were more sensitive in hypertrophy. $K_{dr}$ current density is significantly reduced in hypertrophy without activation and inactivation kinetics. The sensitivity of $K_{dr}$ current on 4-AP is significantly increased in hypertrophy. This is the first study to report evidence for alterations of $K_{Ca}$ channels and $K_{dr}$ channels in coronary SMCs with LVH. The findings may provide some insight into mechanism of the reduced coronary reserve in LVH.

• Journal of Chest Surgery
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• 제35권2호
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• pp.161-165
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• 2002

하행 괴사성 종격동염은 대부분 경부 부위의 농양으로 시작하여 종격동으로 파급되는 매우 치명적인 질환이며 저자에 따라 25∼40%의 사망률을 보고하고 있다. 빠른 진단과 적절한 수술적 치료가 중요하며 수술적 치료의 방법에는 아직 여러 가지 방법들이 보고되고 있지만 농양의 완전한 배농이 특히 중요하다고 보고하고 있다. 배농술은 경부 절개를 통한 배농과 함께 흉부 내의 종격동 배농술이 필요하며 종격동 배농술은 대부분 개흉술을 통하여 시행되어 왔으나 개흉술에 따른 합병증과 수술부위의 감염 등이 술후 이환율을 증가시키는 원인이 될 수 있다. 반면에 흥강경을 이용한 배농은 경부 배농술 및 흉부 배농술을 동시에 시행할 수 있으며 좋은 수술시야를 보여주고 술후 환자의 회복이 빨라 하행 괴사성 종격동염의 좋은 치료 방법이라 생각된다.

• 대한미생물학회지
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• 제35권2호
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• pp.117-127
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• 2000

The non-ELR-containing CXC chemokines Mig and IP-10 have been shown to function as chemotactic cytokines for activated T lymphocytes. In this study, we examined the potential involvement of Mig and IP-10 in antimycobacterial response of mice immunized or infected with M. bovis BCG. The accumulation of Mig and IP-10 mRNA in resident peritoneal monocytes ($RPM{\Phi}$) was slightly reduced by stimulation with vBCG, and the degree was greater for 24 hr culture even though IFN-${\gamma}$ was added. Expression of Mig, IP-10, and IFN-${\gamma}$ in 24 hr delayed-type hypersensitivity (DTH) response was stronger in vBCG-immune mice than in the non-immune. The increase of DTH measured by foot-pad thickness appears to be clearly related to the levels of chemokines Mig and IP10 messages and those of IFN-${\gamma}$ and IL-12. Stimulation with vBCG for 2 days decreased or completely dropped the levels of Mig message in non-immune or immune splenocytes, respectively, whereas IP-10 message was slightly decreased in 2 days culture. Moreover, messages for IL-12 (p40) showed similar kinetics for Mig. The levels of Mig and IP-10 mRNA during the course of infection with BCG were not readily changed in lungs, livers, and spleens from BCG-infected mice. Although there was no obvious changes of Mig and IP-10 messages in the target organs during infection process, we found that the infection progressed over the first 3 wk before being contained by the emerging immune response suggested from detectable amount of IFN-${\gamma}$ mRNA around this time. In view of selectivity of chemokines Mig and IP-10 for activated T cells, these data suggest that chemokine Mig and IP-10, especially in collaboration with IL-12 and IFN-${\gamma}$, may playa role as T cell recruiters in immune response against mycobacterial infection.

• Biomolecules & Therapeutics
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• 제23권4호
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• pp.350-356
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• 2015

Functional dyspepsia (FD) is a prevalent idiopathic upper gastrointestinal (GI) disorder characterized by diverse symptomatology including epigastric pain or discomfort, postprandial fullness, and early satiety. Although its pathophysiological mechanisms have not yet been fully established, the available studies suggest that the etiology of FD is invariably multifactorial. Benachio-F$^{(R)}$ (BF) is a proprietary liquid formulation of 7 herbal extracts that has been proposed to address this multifactorial etiology using multi-drug phytotherapy. The pharmacological effects of BF, in comparison with those of two other herbal products (Whalmyungsu$^{(R)}$; WM and Iberogast$^{(R)}$; IB) were evaluated in rats. In a laparotomy-induced rat model of delayed GI transit, BF significantly accelerated the delayed gastric emptying caused by morphine, apomorphine, and cisplatin, and also significantly increased mean gastric transit, as compared to the control animals. BF markedly increased gastric accommodation in rats and produced higher gastric volume values than did the control treatment. The effects of BF were generally comparable or superior to those of WM and IB in these models. Furthermore, BF significantly stimulated biliary flow, as compared to the control treatment. These results indicated that BF might have great potential as an effective phytotherapeutic agent capable of reducing GI symptoms and increasing quality of life in FD patients.

• 자원환경지질
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• 제55권6호
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• pp.563-570
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• 2022

이 조사는 지상에 존재하는 지하수의 잠재오염원(저장탱크)으로부터 오염물질 누출 시, 이를 조기 진단하기 위한 지하수 오염관측망의 설계 인자로서 부지 규모 지하수환경의 시공간적 변동성을 파악하고자 시행되었다. 부지 내 위치한 저장탱크 주변에 22~25 m 심도로 3개의 관측공을 설치하고, 이들로부터 약 22개월 동안 2분 간격으로 지하수위와 수온 변화를 관측하였으며, 이 자료는 주변 기상관측소의 강수 및 기온 자료와 비교 분석되었다. 조사기간 동안 지하수위의 연 변화와 강수 현상에 대한 반응, 지하수온의 변동과 기온에 대한 지연시간 등은 비교적 작은 규모의 부지에서도 지하수 흐름과 유동 경로의 복잡성을 지시한다. 따라서 오염누출 감시를 위한 지하수 관측망은 상세한 부지특성화 조사 결과에 근거하여 부지 지하수환경의 복잡성을 충분히 고려하여 설계되어야 한다.

• 수산해양기술연구
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• 제25권2호
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• pp.87-104
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• 1989

Recently, with the tendency of more lightening, high-strength and high-speed in the marine industries such as marine structures, ships and chemical plants, the use of the aluminium Alloy is rapidly enlarge and there occurs much interest in the study of corrosion fatigue crack characteristics. In this paper, the initiation of surface crack and the propagation characteristics on the base metal and weld zone of 5086-H116 Aluminium Alloy Plate which is one of the Al-Mg serious alloy(A5000serious) used most when building the special vessels, were investigated by the plane bending corrosion fatigue under the environments of marine, air and applying cathodic protection. The effects of various specific resistances on the initiation, propagation behavior of corrosion fatigue crack and corrosion fatigue life in the base metal and heat affected zone were examined and its corrosion sensitivity was quantitatively obtained. The effects of corrosion on the crack depth in relation to the uniform surface crack length were also investigated. Also, the structural, mechanical and electro-chemical characteristics of the metal at the weld zone were inspected to verify the reasons of crack propagation behavior in the corrosion fatigue fracture. In addition, the effect of cathodic protection in the fracture surface of weld zone was examined fractographically by Scanning Electron Microscope(S.E.M.). The main results obtained are as follows; (1) The initial corrosion fatigue crack sensitibity under specific resistance of 25Ω.cm% show 2.22 in the base metal and 19.6 in the HEZ, and the sensitivity decreases as specific resistance increases (2) By removing reinforcement of weldment, the initiation and propagation of corrosion crack in the HAZ are delayed, and corrosion fatigue life increases. (3) As specific resistance decreases, the sensitivity difference of corrosion fatigue life in the base metal and HAZ is more susceptible than that of intial corrosion fatigue crack. (4) Experimental constant, m(Paris' rule) in the marine environment is in the range of about 3.69 to 4.26, and as specific resistance increases, thje magnitude of experimental constant, also increases and the effect by corrosion decreases. (5) Comparing surface crack length with crack depth, the crack depth toward the thickness of specimen in air is more deeply propagated than that in corrosion environment. (6) The propagation particulars of corrosion fatigue crack for HAZ under initial stress intensity factor range of $\Delta$k sub(li) =27.2kgf.mm super(-3/2) and stress ratio of R=0 shows the retardative phenomenon of crack propagation by the plastic deformation at crack tip. (7) Number of stress cycles to corrosion fatigue crack initiation of the base metal and the welding heat affected zone are delayed by the cathodic protection under the natural sea water. The cathodic protection effect for corrosion fatigue crack initiation is eminent when the protection potential is -1100 mV(SCE). (8) When the protection potential E=-1100 mV(SCE), the corrosion fatigue crack propagation of welding heat affected zone is more rapid than that of the case without protection, because of the microfissure caused by welding heat cycle.

• The Korean Journal of Physiology and Pharmacology
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• 제21권2호
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• pp.259-265
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• 2017

Excessive influx and the subsequent rapid cytosolic elevation of $Ca^{2+}$ in neurons is the major cause to induce hyperexcitability and irreversible cell damage although it is an essential ion for cellular signalings. Therefore, most neurons exhibit several cellular mechanisms to homeostatically regulate cytosolic $Ca^{2+}$ level in normal as well as pathological conditions. Delayed rectifier $K^+$ channels ($I_{DR}$ channels) play a role to suppress membrane excitability by inducing $K^+$ outflow in various conditions, indicating their potential role in preventing pathogenic conditions and cell damage under $Ca^{2+}$-mediated excitotoxic conditions. In the present study, we electrophysiologically evaluated the response of $I_{DR}$ channels to hyperexcitable conditions induced by high $Ca^{2+}$ pretreatment (3.6 mM, for 24 hours) in cultured hippocampal neurons. In results, high $Ca^{2+}$-treatment significantly increased the amplitude of $I_{DR}$ without changes of gating kinetics. Nimodipine but not APV blocked $Ca^{2+}$-induced $I_{DR}$ enhancement, confirming that the change of $I_{DR}$ might be targeted by $Ca^{2+}$ influx through voltage-dependent $Ca^{2+}$ channels (VDCCs) rather than NMDA receptors (NMDARs). The VDCC-mediated $I_{DR}$ enhancement was not affected by either $Ca^{2+}$-induced $Ca^{2+}$ release (CICR) or small conductance $Ca^{2+}$-activated $K^+$ channels (SK channels). Furthermore, PP2 but not H89 completely abolished $I_{DR}$ enhancement under high $Ca^{2+}$ condition, indicating that the activation of Src family tyrosine kinases (SFKs) is required for $Ca^{2+}$-mediated $I_{DR}$ enhancement. Thus, SFKs may be sensitive to excessive $Ca^{2+}$ influx through VDCCs and enhance $I_{DR}$ to activate a neuroprotective mechanism against $Ca^{2+}$-mediated hyperexcitability in neurons.

• 한국농공학회지
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• 제28권3호
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• pp.79-88
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• 1986

Drought occurs most frequently and severely around transplanting season of the rice plants in Korea. Shortage of water due to drought for the paddy fields often delays transplanting, and less often the rice plants are subjected to water stress after delayed transplanting. The present study aimed at quantification of the rice crop loss due to delayed transplanting, different inten3ity of water stress, and the combined effect of delay in transplanting followed by water stress for better use of limited water for irrigation under drought. The rice variety Chucheong, a japonica, and Nampung, an indica x japonica, were grown, transplanted to 1/200 a plastic pots, and subjected to different timing of transplanting and degree of water stress under a rainfall autosersing, sliding clear plastic roof facility with completely randomized arrangement of 5 replications. The results obtained are summarized as follows: 1.Twelve days or 22 days delay in transplanting without water stress reduced rice yield by 25% and 43% in the japonica variety, and by 15% and 60% in the indica x japonica variety. 2.The 10 days or 20 days water stress developed without irrigation after drainage in the rice plants transplanted at proper time lowered the water potential at the paddy soil 10cm deep to -4 bar, and -12 bar and caused rice yield reduction by 14%, and 45% in the japonica variety and by 8%, and 50% in the indica X japonica variety. 3.The 12 days delay in transplanting and 10 days or 20 days water stress reduced rice yield by 39% and 59% in the japonica variety, and by 38% and 52% in the indica x japonica variety. The 22 days delay in transplanting plus 10 days water stress caused yield reduction by 76%, i.e. meaningless yield, in both varieties. 4.The intermittent irrigation just to wet the soil body for 10 days after 10 days water stress without irrigation increased rece yield by 12 to 16% compared to the rice plants water stessed without irrigation continuously for 20 days in both varieties respectively. 5.The above results suggest strongly 1) to transplant the rice plants at proper .time even with some water stress rather than delay for sufficient water from later rainfall, and 2) to distribute insufficient irrigation water to broader area of transplanted rice with limited irrigation for better use of limited irrigation water. A greater sensitivity of japonica variety to a moderate water stress than the indica X japonica variety during initial rooting and tillering stage was noticed. To cope with frequent drought in rice culture, firstly the lasting time of transplanting without yield reduction should be clarified by region and variety, and secondly a scheme of rational distribution of limited water should be developed by region with better knowledge on the varietal distribution of limited water should be developed by region with better knowledge on the varietal responses to varying intensity of water stress.

• 한국농공학회지
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• 제30권2호
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• pp.31-43
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• 1988

The Purpose of this study is to develop the rainfall-delayed response model (RDR Model) which influences the baseflow proportion of rivers as a result of the antecedent precipitation of the previous several months. The assesment of accurate baseflows in the rivers is one of the most important elements for the planning of seasonal water supply for agriculture, water resources development, hydrological studies for the availability of water and design criteria for various irrigation facilities. The Palukan river gauging site which is located in the Pulukan catchment on Bali Island, Indonesia was selected to develop this model. The basic data which has been used comprises the available historic flow records at 19 hydrologic gauging stations and 77 rainfall stations on Bali Island in the study. The methology adopted for the derivation of the RDR model was the water balance equation which is commonly used for any natural catcbment ie.P=R+(catchment losses) -R+(ET+DP+DSM+DGW). The catchment losses consist of evapotranspiration, deep percolation. change in soil moisture, and change in groundwater storage. The catchment areal rainfall has been generated by applying the combination method of Thiessen polygon and Isohyetal lines in the studies. The results obtained from the studies may be summarized as follows ; 1. The rainfall-runoff relationship derived from the water balance equation is as shown below, assuming a relationship of the form Y=AX+B. Finally these two equations for the annual runoff were derived ; ARO$_1$=0.855 ARF-821, ARF>=l,400mm ARO$_2$=0.290ARF- 33, ARF<1,400mm 2. It was found that the correction of observed precipitation by a combination of Thiessen polygons and Isohyetal lines gave good correlation. 3. Analysis of historic flow data and rainfall, shows that surface runoff and base flow are 52 % and 48% (equivalent to 59.4 mm) of the annual runoff, respectively. 4. Among the eight trial RDR models run, Model C provided the correlation with historic flow data. The number of months over which baseflow is distributed and the relative proportions of rainfall contributing in each month, were estimated by performing several trial runs using data for the Pulukan catchment These resulted in a value for N of 4 months with contributing proportions of 0.45, 0.50, 0.03 and 0.02. Thus the baseflow in any month is given by : P$_1$(n) =0.45 P(n) +0.50 P(n-I ) +0.03 P(n-$_2$) +0.02 P(n-$_3$) 5. The RDR model test gave estimated flows within +3.4 % and -1.0 % of the observed flows. 6. In the case of 3 consecutive no rain months, it was verified that 2.8 % of the dependable annual flow will be carried over the following year and 5.8 % of the potential annual baseflow will be transfered to the next year as a result of the rainfall-delayed response. The results of evaluating the pefformance of the RDR Model was generally satisfactory.

• 생명과학회지
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• 제19권10호
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• pp.1484-1488
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• 2009

가족성 저칼륨성 주기성 마비란 상염색체 우성 유전 질환으로 저칼륨혈증을 동반한 간헐적인 가역적 이완성 근육 마비를 특징으로 한다. 세포내 저류된 칼륨으로 인해 저칼륨혈증이 지속되고 근세포 활성이상으로 인해 마비가 발생하는 것으로 알려져 있다. 이러한 증상발현의 분자생물학적 기전을 확인하기 위해 세포 내 칼륨이온을 세포 밖으로 이동시키는 지연성 정류형 채널 단백질의 일종인 KCNQ3와 KCNQ5를 대상으로, 정상인과 환자에서 채취한 골격근 세포를 생리적 세포외 정상 칼륨농도인 4 mM과 탈분극 유도를 위한 고칼륨농도인 50mM에 노출시켜 단백질의 양적 변화 유무를 확인하였다. 유전자 발현양상을 확인하기 위해 mRNA의 양적 변화를 확인한 결과 모든 조건에서 유의한 변화가 관찰되지 않아 정상 칼륨조건과 고칼륨조건이 두 유전자발현의 변화를 야기하지 않음을 확인하였다. 그러나 단백질 양을 관찰한 결과 환자의 골격근 세포가 50 mM의 칼륨농도에 노출되는 경우 KCNQ3 단백질은 세포질 내에서 증가하고 세포막 내에서 감소하였다. 이는 환자의 골격근 세포가 고농도의 세포외 칼륨에 의해 탈분극 되는 경우 재분극에 중요한 기능을 담당하는 KCNQ3 채널 단백질이 세포질 내로 이동하여 재분극 형성의 장애를 초래하고 이로 인해 근세포 활성이 일어나지 않게 되어 마비를 유발할 수 있음을 시사하는 결과로 본 질환의 새로운 발병 기전을 설명할 수 있는 근거로 생각된다.